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O88780

- KLK8_RAT

UniProt

O88780 - KLK8_RAT

Protein

Kallikrein-8

Gene

Klk8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury By similarity.By similarity

    Catalytic activityi

    Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Charge relay systemBy similarity
    Active sitei120 – 1201Charge relay systemBy similarity
    Active sitei212 – 2121Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. cell death Source: UniProtKB
    2. keratinocyte proliferation Source: UniProtKB
    3. memory Source: UniProtKB
    4. negative regulation of axon regeneration Source: UniProtKB
    5. negative regulation of myelination Source: UniProtKB
    6. neuron projection morphogenesis Source: UniProtKB
    7. regulation of synapse organization Source: UniProtKB
    8. response to wounding Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.118. 5301.

    Protein family/group databases

    MEROPSiS01.244.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein-8 (EC:3.4.21.118)
    Alternative name(s):
    Brain serine protease 1
    Neuropsin
    Short name:
    NP
    Serine protease 19
    Gene namesi
    Name:Klk8
    Synonyms:Bsp1, Nrpn, Prss19
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1305998. Klk8.

    Subcellular locationi

    Secreted By similarity. Cytoplasm By similarity
    Note: Shows a cytoplasmic distribution in the keratinocytes.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Propeptidei29 – 324By similarityPRO_0000027950
    Chaini33 – 260228Kallikrein-8PRO_0000027951Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi39 ↔ 173PROSITE-ProRule annotation
    Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi145 ↔ 246PROSITE-ProRule annotation
    Disulfide bondi152 ↔ 218PROSITE-ProRule annotation
    Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
    Disulfide bondi208 ↔ 233PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiO88780.

    Expressioni

    Tissue specificityi

    Restricted to hippocampus.

    Gene expression databases

    GenevestigatoriO88780.

    Interactioni

    Subunit structurei

    Interacts with SPINK9.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO88780.
    SMRiO88780. Positions 33-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 257225Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiO88780.
    PhylomeDBiO88780.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88780-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRPPPCAIQ TWILLFLLMG AWAGLTRAQG SKILEGQECK PHSQPWQTAL    50
    FQGERLVCGG VLVGDRWVLT AAHCKKDKYS VRLGDHSLQK RDEPEQEIQV 100
    ARSIQHPCFN SSNPEDHSHD IMLIRLQNSA NLGDKVKPIE LANLCPKVGQ 150
    KCIISGWGTV TSPQENFPNT LNCAEVKIYS QNKCERAYPG KITEGMVCAG 200
    SSNGADTCQG DSGGPLVCNG VLQGITTWGS DPCGKPEKPG VYTKICRYTN 250
    WIKKTMGKRD 260
    Length:260
    Mass (Da):28,510
    Last modified:November 1, 1998 - v1
    Checksum:i58DF4F0602A0B7F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005641 mRNA. Translation: CAA06643.1.
    UniGeneiRn.77746.

    Genome annotation databases

    UCSCiRGD:1305998. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005641 mRNA. Translation: CAA06643.1 .
    UniGenei Rn.77746.

    3D structure databases

    ProteinModelPortali O88780.
    SMRi O88780. Positions 33-256.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.244.

    Proteomic databases

    PRIDEi O88780.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:1305998. rat.

    Organism-specific databases

    RGDi 1305998. Klk8.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi O88780.
    PhylomeDBi O88780.

    Enzyme and pathway databases

    BRENDAi 3.4.21.118. 5301.

    Miscellaneous databases

    PROi O88780.

    Gene expression databases

    Genevestigatori O88780.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer.
      Tissue: Brain.

    Entry informationi

    Entry nameiKLK8_RAT
    AccessioniPrimary (citable) accession number: O88780
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3