Kallikrein-8 precursor - Rattus norvegicus (Rat)

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O88780 (KLK8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kallikrein-8
EC=3.4.21.118

Alternative name(s):
Brain serine protease 1
Neuropsin
Short name=NP
Serine protease 19

Gene names

Name:	Klk8
Synonyms:	Bsp1, Nrpn, Prss19

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury By similarity.
Catalytic activity	Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys.
Subunit structure	Interacts with SPINK9 By similarity.
Subcellular location	Secreted By similarity. Cytoplasm By similarity. Note: Shows a cytoplasmic distribution in the keratinocytes By similarity.
Tissue specificity	Restricted to hippocampus.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell death Inferred from sequence or structural similarity. Source: UniProtKB keratinocyte proliferation Inferred from sequence or structural similarity. Source: UniProtKB memory Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of axon regeneration Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of myelination Inferred from sequence or structural similarity. Source: UniProtKB neuron projection morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of synapse organization Inferred from sequence or structural similarity. Source: UniProtKB response to wounding Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	serine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Propeptide

29 – 32

By similarity

PRO_0000027950

Chain

33 – 260

228

Kallikrein-8

PRO_0000027951

Regions

Domain

33 – 257

225

Peptidase S1

Sites

Active site

Charge relay system By similarity

Active site

120

Charge relay system By similarity

Active site

212

Charge relay system By similarity

Amino acid modifications

Glycosylation

110

N-linked (GlcNAc...) Potential

Disulfide bond

39 ↔ 173

By similarity

Disulfide bond

58 ↔ 74

By similarity

Disulfide bond

145 ↔ 246

By similarity

Disulfide bond

152 ↔ 218

By similarity

Disulfide bond

184 ↔ 198

By similarity

Disulfide bond

208 ↔ 233

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O88780 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 58DF4F0602A0B7F5
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FASTA

260

28,510

        10         20         30         40         50         60 
MGRPPPCAIQ TWILLFLLMG AWAGLTRAQG SKILEGQECK PHSQPWQTAL FQGERLVCGG 

        70         80         90        100        110        120 
VLVGDRWVLT AAHCKKDKYS VRLGDHSLQK RDEPEQEIQV ARSIQHPCFN SSNPEDHSHD 

       130        140        150        160        170        180 
IMLIRLQNSA NLGDKVKPIE LANLCPKVGQ KCIISGWGTV TSPQENFPNT LNCAEVKIYS 

       190        200        210        220        230        240 
QNKCERAYPG KITEGMVCAG SSNGADTCQG DSGGPLVCNG VLQGITTWGS DPCGKPEKPG 

       250        260 
VYTKICRYTN WIKKTMGKRD

« Hide

References

[1]	"Serine proteases in rodent hippocampus." Davies B.J., Pickard B.S., Steel M., Morris R.G.M., Lathe R. J. Biol. Chem. 273:23004-23011(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005641 mRNA. Translation: CAA06643.1.
IPI	IPI00212573.
UniGene	Rn.77746.
3D structure databases
ProteinModelPortal	O88780.
SMR	O88780. Positions 33-256.
ModBase	Search...
Protein family/group databases
MEROPS	S01.244.
Proteomic databases
PRIDE	O88780.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:1305998. rat.
Organism-specific databases
RGD	1305998. Klk8.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	O88780.
OrthoDB	EOG4XKV7F.
Enzyme and pathway databases
BRENDA	3.4.21.118. 5301.
Gene expression databases
Genevestigator	O88780.
GermOnline	ENSRNOG00000018580. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK8_RAT

Accession

Primary (citable) accession number: O88780

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents