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O88780 (KLK8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-8

EC=3.4.21.118
Alternative name(s):
Brain serine protease 1
Neuropsin
Short name=NP
Serine protease 19
Gene names
Name:Klk8
Synonyms:Bsp1, Nrpn, Prss19
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury By similarity.

Catalytic activity

Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys.

Subunit structure

Interacts with SPINK9 By similarity.

Subcellular location

Secreted By similarity. Cytoplasm By similarity. Note: Shows a cytoplasmic distribution in the keratinocytes By similarity.

Tissue specificity

Restricted to hippocampus.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Propeptide29 – 324 By similarity
PRO_0000027950
Chain33 – 260228Kallikrein-8
PRO_0000027951

Regions

Domain33 – 257225Peptidase S1

Sites

Active site731Charge relay system By similarity
Active site1201Charge relay system By similarity
Active site2121Charge relay system By similarity

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Disulfide bond39 ↔ 173 By similarity
Disulfide bond58 ↔ 74 By similarity
Disulfide bond145 ↔ 246 By similarity
Disulfide bond152 ↔ 218 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond208 ↔ 233 By similarity

Sequences

Sequence LengthMass (Da)Tools
O88780 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 58DF4F0602A0B7F5

FASTA26028,510
        10         20         30         40         50         60 
MGRPPPCAIQ TWILLFLLMG AWAGLTRAQG SKILEGQECK PHSQPWQTAL FQGERLVCGG 

        70         80         90        100        110        120 
VLVGDRWVLT AAHCKKDKYS VRLGDHSLQK RDEPEQEIQV ARSIQHPCFN SSNPEDHSHD 

       130        140        150        160        170        180 
IMLIRLQNSA NLGDKVKPIE LANLCPKVGQ KCIISGWGTV TSPQENFPNT LNCAEVKIYS 

       190        200        210        220        230        240 
QNKCERAYPG KITEGMVCAG SSNGADTCQG DSGGPLVCNG VLQGITTWGS DPCGKPEKPG 

       250        260 
VYTKICRYTN WIKKTMGKRD 

« Hide

References

[1]"Serine proteases in rodent hippocampus."
Davies B.J., Pickard B.S., Steel M., Morris R.G.M., Lathe R.
J. Biol. Chem. 273:23004-23011(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Fischer.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005641 mRNA. Translation: CAA06643.1.
UniGeneRn.77746.

3D structure databases

ProteinModelPortalO88780.
SMRO88780. Positions 33-256.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.244.

Proteomic databases

PRIDEO88780.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:1305998. rat.

Organism-specific databases

RGD1305998. Klk8.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidO88780.
PhylomeDBO88780.

Enzyme and pathway databases

BRENDA3.4.21.118. 5301.

Gene expression databases

GenevestigatorO88780.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO88780.

Entry information

Entry nameKLK8_RAT
AccessionPrimary (citable) accession number: O88780
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries