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O88778 (BSN_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Protein bassoon
Gene names
Name:Bsn
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length3938 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses By similarity. Ref.5

Subunit structure

Interacts with ERC2/CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Ref.4 Ref.5

Subcellular location

Cytoplasm. Cell junction › synapse › synaptosome. Cytoplasm › cytoskeleton. Note: Localized to the active zone of presynaptic density. In retina, is localized in the outer plexiform layer at ribbon synapses formed by rods and cones but was absent from basal synaptic contacts formed by cones. In the retinal inner plexiform layer localized to conventional inhibitory GABAergic synapses, made by amacrine cells, but absent from the bipolar cell ribbon synapses. Ref.1 Ref.2 Ref.3

Developmental stage

Detected at embryonic day E18 and at later stages. The expression does not significantly change during the developmental stages tested. Ref.4

Post-translational modification

Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 39383937Protein bassoon
PRO_0000065004

Regions

Repeat568 – 57471
Repeat575 – 58172
Repeat582 – 58873
Zinc finger167 – 19024C4-type Potential
Zinc finger195 – 21723C4-type Potential
Zinc finger462 – 48524C4-type Potential
Zinc finger490 – 51223C4-type Potential
Region62 – 7094 X 2 AA tandem repeats of P-G
Region568 – 588213 X 7 AA tandem repeats of K-A-S-P-Q-A-[AK]
Region2934 – 299663Sufficient for binding to ERC2
Coiled coil1032 – 108756 Potential
Coiled coil1176 – 120328 Potential
Coiled coil2345 – 2470126 Potential
Coiled coil2933 – 297543 Potential
Coiled coil3772 – 380332 Potential
Compositional bias2594 – 26007Poly-Arg
Compositional bias2621 – 26266Poly-Arg
Compositional bias2647 – 26548Poly-Ala
Compositional bias3770 – 379728Poly-Gln

Amino acid modifications

Modified residue61Phosphoserine By similarity
Modified residue1051Phosphoserine By similarity
Modified residue1351Phosphoserine By similarity
Modified residue1401Phosphoserine By similarity
Modified residue1421Phosphoserine By similarity
Modified residue9651Phosphoserine By similarity
Modified residue10041Phosphoserine By similarity
Modified residue10871Phosphothreonine By similarity
Modified residue10901Phosphoserine By similarity
Modified residue10931Phosphoserine By similarity
Modified residue10991Phosphoserine By similarity
Modified residue11011Phosphothreonine By similarity
Modified residue11211Phosphotyrosine By similarity
Modified residue12211Phosphoserine By similarity
Modified residue14691Phosphoserine By similarity
Modified residue14701Phosphoserine By similarity
Modified residue14741Phosphoserine By similarity
Modified residue14811Phosphoserine By similarity
Modified residue14831Phosphothreonine By similarity
Modified residue14851Phosphoserine By similarity
Modified residue14981Phosphoserine By similarity
Modified residue15411Phosphoserine By similarity
Modified residue15421Phosphoserine By similarity
Modified residue20171Phosphoserine By similarity
Modified residue20321Phosphotyrosine By similarity
Modified residue20611Phosphotyrosine By similarity
Modified residue21121Phosphoserine By similarity
Modified residue25641Phosphoserine By similarity
Modified residue25811Phosphothreonine By similarity
Modified residue26081Phosphothreonine By similarity
Modified residue26791Phosphoserine By similarity
Modified residue26881Phosphothreonine By similarity
Modified residue27931Phosphoserine By similarity
Modified residue27961Phosphoserine By similarity
Modified residue28071Phosphoserine By similarity
Modified residue28431Phosphoserine By similarity
Modified residue28451Phosphoserine By similarity
Modified residue28511Phosphoserine By similarity
Modified residue28931Phosphoserine By similarity
Modified residue30071Phosphoserine By similarity
Modified residue32861Phosphoserine By similarity
Modified residue33681Phosphoserine By similarity
Modified residue34171Phosphotyrosine By similarity
Modified residue34181Phosphotyrosine By similarity
Modified residue34451Phosphotyrosine By similarity
Lipidation21N-myristoyl glycine Ref.3
Glycosylation13391O-linked (GlcNAc) Ref.6
Glycosylation13801O-linked (GlcNAc) By similarity
Glycosylation19221O-linked (GlcNAc) By similarity
Glycosylation23071O-linked (GlcNAc) By similarity
Glycosylation25101O-linked (GlcNAc) By similarity
Glycosylation26851O-linked (GlcNAc) By similarity
Glycosylation29301O-linked (GlcNAc) By similarity

Experimental info

Mutagenesis21G → A: Loss of myristoylation. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O88778 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5BF3C230E2C71AE2

FASTA3,938418,424
        10         20         30         40         50         60 
MGNEASLEGG AGEGPLPPGG SGLGPGPGAG KPPSALAGGG QLPVAGAARA AGPPTPGLGL 

        70         80         90        100        110        120 
VPGPGPGPGP GSVSRRLDPK EPLGSQRATS PTPKQASATA PGRESPRETR AQGLSGQEAE 

       130        140        150        160        170        180 
GPRRTLQVDS RTQRSGRSPS VSPDRGSTPT SPYSVPQIAP LPSSTLCPIC KTSDLTSTSS 

       190        200        210        220        230        240 
QPNFNTCTQC HNKVCNQCGF NPNPHLTQVK EWLCLNCQMQ RALGMDMTTA PRSKSQQQLH 

       250        260        270        280        290        300 
SPALSPAHSP AKQPLGKPEQ ERSRSPGATQ SGPRQAEAAR ATSVPGPTQA TAPPEVGRVS 

       310        320        330        340        350        360 
PQPPLSTKPS TAEPRPPAGE AQGKSATTVP SGLGAAEQTQ GGLTGKLFGL GASLLTQAST 

       370        380        390        400        410        420 
LMSVQPEADT QGQPSPSKGP PKIVFSDASK EAGPRPPGSG PGPGPTPGAK TEPGPRTGPG 

       430        440        450        460        470        480 
SGPGALAKTG GTPSPKHGRA DHQAASKAAA KPKTMPKERA ACPLCQAELN VGSRGPANYN 

       490        500        510        520        530        540 
TCTACKLRVC TLCGFNPTPH LVEKTEWLCL NCQTKRLLEG SLGEPAPLPL PTPQEPPAGV 

       550        560        570        580        590        600 
PQRAAGASPL KQKGPQGPGQ PSGSLPPKAS PQAAKASPQA AKASPQAKPL RASEPSKTSS 

       610        620        630        640        650        660 
SAPEKKTGIP VKAEPVPKPP PETAVPPGTP KAKSGVKRTD PATPVVKPVP EAPKSGEAEE 

       670        680        690        700        710        720 
PVPKPYSQDL SRSPQSLSDT GYSSDGVSSS QSEITGVVQQ EVEQLDSAGV TGPRPPSPSE 

       730        740        750        760        770        780 
LHKVGSSMRP SLEAQAVAPS GEWSKPPSGS AVEDQKRRPH SLSIMPEAFD SDEELGDILE 

       790        800        810        820        830        840 
EDDSLAMGRQ REQQDTAESS DDFGSQLRHD YVEDSSEGGL SPLPPQPPAR ADMTDEEFMR 

       850        860        870        880        890        900 
RQILEMSAEE DNLEEDDTAV SGRGLAKHGA QKASARPRPE SSQESVALPK RRLPHNATTG 

       910        920        930        940        950        960 
YEELLSEEGP AEPTDGALQG GLRRFKTIGL NSTGRLWSTS LDLGQGSDPN LDREPELEME 

       970        980        990       1000       1010       1020 
SLTGSPEDRS RGEHSSTLPA STPSYTSGTS PTSLSSLEED SDSSPSRRQR LEEAKQQRKA 

      1030       1040       1050       1060       1070       1080 
RHRSHGPLLP TIEDSSEEEE LREEEELLRE QEKMREVEQQ RIRSTARKTR RDKEELRAQR 

      1090       1100       1110       1120       1130       1140 
RRERSKTPPS NLSPIEDASP TEELRQAAEM EELHRSSCSE YSPSPSLDSE AETLDGGPTR 

      1150       1160       1170       1180       1190       1200 
LYKSGSEYNL PAFMSLCSPT ETPSGSSTTP SSGRPLKSAE EAYEDMMRKA ELLQRQQGQA 

      1210       1220       1230       1240       1250       1260 
AGARGPHGGP SQPTGPRSQG SFEYQDTLDH DYGGRASQPA ADGTPAGLGA TVYEEILQTS 

      1270       1280       1290       1300       1310       1320 
QSIARMRQAS SRDLAFTEDK KKEKQFLNAE SAYMDPMKQN GGPLTPGTSP TQLAAPVSFP 

      1330       1340       1350       1360       1370       1380 
TSTSSDSSGG RVIPDVRVTQ HFAKEPQEPL KLHSSPASPS LASKEVGMTF SQGPGTPATT 

      1390       1400       1410       1420       1430       1440 
AMAPCPASLP RGYMTPAGPE RSPSTSSTIH SYGQPPTTAN YGSQTEELPH APSGPAGSGR 

      1450       1460       1470       1480       1490       1500 
ASREKPLSGG DGEVGPPQPS RGYSYFTGSS PPLSPSTPSE SPTFSPSKLG PRATAEFSTQ 

      1510       1520       1530       1540       1550       1560 
TPSLTPSSDI PRSVGTPSPM VAQGTQTPHR PSTPRLVWQQ SSQEAPVMVI TLASDASSQT 

      1570       1580       1590       1600       1610       1620 
RMVHASASTS PLCSPTDSQP ASHSYSQTTP PSASQMPSEP AGPPGFPRAP SAGVDGPLAL 

      1630       1640       1650       1660       1670       1680 
YGWGALPAEN ISLCRISSVP GTSRVEPGPR PPGTAVVDLR TAVKPTPIIL TDQGMDLTSL 

      1690       1700       1710       1720       1730       1740 
AVEARKYGLA LDPVPGRQST AVQPLVINLN AQEQTHTFLA TATTVSITMA SSVLMAQQKQ 

      1750       1760       1770       1780       1790       1800 
PVVYGDPFQS RLDFGQGSGS PVCLAQVKQV EQAVQTAPYR GGPRGRPREA KFARYNLPNQ 

      1810       1820       1830       1840       1850       1860 
VTPLARRDIL ITQMGTAQSV SLKPGPVPEP GAEPHRATPA ELRAHALPGT RKPHTVVVQM 

      1870       1880       1890       1900       1910       1920 
GEGAAGTVTT LLPEEPAGAL DLTGMRPESR LACCDMAYKF PFGSSCTGTF HPAPSAPDKS 

      1930       1940       1950       1960       1970       1980 
VTDAALPGQS SGPFYSPRDP EPPEPLTFRA QGVVGPGPHE EQRPYPQGLP GRLYSSMSDT 

      1990       2000       2010       2020       2030       2040 
NLAEAGLNYH AQRIGQLFQG PGRDSAVDLS SLKHSYSLGF ADGRYLGQGL QYGSFTDLRH 

      2050       2060       2070       2080       2090       2100 
PTDLLSHPLP MRPYSSVSNI YSDHRYGPRG DAVGFQEASL AQYSATTARE ISRMCAALNS 

      2110       2120       2130       2140       2150       2160 
MDQYGGRHGG GSGGPDLVPY QPQHGPGLNA PQGLASLRSG LLGNPTYPEG QPSPGNLAQY 

      2170       2180       2190       2200       2210       2220 
GPAASQGTAV RQLLPSTATV RAADGMIYST INTPIAATLP ITTQPASVLR PMVRGGMYRP 

      2230       2240       2250       2260       2270       2280 
YGSGGVTAVP LTSLTRVPMI APRVPLGPAG LYRYPAPSRF PIASTIPPAE GPVYLGKPAA 

      2290       2300       2310       2320       2330       2340 
AKASGAGGPP RPELPAGGAR EEPLSTTAPP AVIKEAPVAQ APAPPPGQKP AGDAAAGSGS 

      2350       2360       2370       2380       2390       2400 
GVLGRPVMEK EEASQEDRQR KQQEQLLQLE RERVELEKLR QLRLQEELER ERVELQRHRE 

      2410       2420       2430       2440       2450       2460 
EEQLLVQREL QELQTIKHHV LQQQQEERQA QFALQREQLA QQRLQLEQIQ QLQQQLQQQL 

      2470       2480       2490       2500       2510       2520 
EEQKQRQKAP FPATCEAPSR GPPPAATELA QNGQYWPPLT HTAFIAVAGT EGPGQAREPV 

      2530       2540       2550       2560       2570       2580 
LHRGLPSSAS DMSLQTEEQW EAGRSGIKKR HSMPRLRDAC EPESGPDPST VRRIADSSVQ 

      2590       2600       2610       2620       2630       2640 
TDDEEGEGRY LLTRRRRTRR SADCSVQTDD EDNAEWEQPV RRRRSRLSRH SDSGSDSKHE 

      2650       2660       2670       2680       2690       2700 
ASASSSAAAA AARAMSSVGI QTISDCSVQT EPEQLPRVSP AIHITAATDP KVEIVRYISA 

      2710       2720       2730       2740       2750       2760 
PEKTGRGESL ACQTEPDGQA QGVAGPQLIG PTAISPYLPG IQIVTPGALG RFEKKKPDPL 

      2770       2780       2790       2800       2810       2820 
EIGYQAHLPP ESLSQLVSRQ PPKSPQVLYS PVSPLSPHRL LDTSFASSER LNKAHVSPQK 

      2830       2840       2850       2860       2870       2880 
QFIADSTLRQ QTLPRPMKTL QRSLSDPKPL SPTAEESAKE RFSLYQHQGG LGSQVSALPP 

      2890       2900       2910       2920       2930       2940 
NGLVRKVKRT LPSPPPEEAH LPLAGQVPSQ LYAASLLQRG LAGPTTVPAT KASLLRELDR 

      2950       2960       2970       2980       2990       3000 
DLRLVEHEST KLRKKQAELD EEEKEIDAKL KYLELGITQR KESLAKDRVG RDYPPLRGLG 

      3010       3020       3030       3040       3050       3060 
EHRDYLSDSE LNQLRLQGCT TPAGQYVDYP ASAAVPATPS GPTAFQQPRF PPAATQYTAG 

      3070       3080       3090       3100       3110       3120 
SSGPTQNGFL AHQAPTYTGP STYPAPTYPP GTSYPAEPGL PSQPAFHPTG HYAAPTPMPT 

      3130       3140       3150       3160       3170       3180 
TQSAPFPVQA DSHAAHQKPR QTSLADLEQK VPTNYEVISS PAVTVSSTPS ETGYSGPAVS 

      3190       3200       3210       3220       3230       3240 
SSYEHGKAPE HPRGGDRSSV SQSPAPTYPS DSHYTSLEQN VPRNYVMIDD ISELTKDSTP 

      3250       3260       3270       3280       3290       3300 
TASDSQRPEP LGPGGVSGRP GKDPGEPAVL EGPTLPCCYG RGEEESEEDS YDPRGKSGHH 

      3310       3320       3330       3340       3350       3360 
RSMESNGRPA STHYYSDSDY RHGARADKYG PGPMGPKHPS KNLAPAAISS KRSKHRKQGM 

      3370       3380       3390       3400       3410       3420 
EQKISKFSPI EEAKDVESDL ASYPPPTVSS SLTSRSRKFQ DEITYGLKKN VYEQQRYYGV 

      3430       3440       3450       3460       3470       3480 
SSRDTAEEDD RMYGGSSRSR VASAYSGEKL SSHDFSSRSK GYERERETAQ RLQKAGPKPS 

      3490       3500       3510       3520       3530       3540 
SLSMAHGRAR PPMRSQASEE ESPVSPLGRP RPAGGALPPG DTCPQFCSSH SMPDVQEHVK 

      3550       3560       3570       3580       3590       3600 
DGPRAHAYKR EEGYILDDSH CVVSDSEAYH LGQEETDWFD KPRDARSDRF RHHGGHTVSS 

      3610       3620       3630       3640       3650       3660 
SQKRGPARHS YHDYDEPPEE GLWPHDEGGP GRHTSAKEHR HHGDHGRHSG RHAGEEPGRR 

      3670       3680       3690       3700       3710       3720 
AARPHARDMG RHETRPHPQA SPAPAMQKKG QPGYPSSADY SQPSRAPSAY HHASDSKKGS 

      3730       3740       3750       3760       3770       3780 
RQAHSGPTVL QPKPEAQAQP QMQGRQAVPG PQQSQPPSSR QTPSGTASRQ PQTQQQQQQQ 

      3790       3800       3810       3820       3830       3840 
QQQQQQQQQQ QQQQQQQGLG QQAPQQAPSQ ARLQQQSQPT TRSTAPAASH PAGKPQPGPT 

      3850       3860       3870       3880       3890       3900 
TAPGPQPAGL PRAEQAGSSK PAAKAPQQGR APQAQSAPGP AGAKTGARPG GTPGAPAGQP 

      3910       3920       3930 
AAEGESVFSK ILPGGAAEQA GKLTEAVSAF GKKFSSFW

« Hide

References

[1]"Bassoon, a novel zinc-finger CAG/Glutamine-repeat protein selectively localized at the active zone of presynaptic nerve terminals."
tom Dieck S., Sanmarti-Vila L., Langnaese K., Richter K., Kindler S., Soyke A., Wex H., Smalla K.-H., Kaempf U., Fraenzer J.-T., Stumm M., Garner C.C., Gundelfinger E.D.
J. Cell Biol. 142:499-509(1998) [PubMed: 9679147] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Differential expression of the presynaptic cytomatrix protein bassoon among ribbon synapses in the mammalian retina."
Brandstatter J.H., Fletcher E.L., Garner C.C., Gundelfinger E.D., Wassle H.
Eur. J. Neurosci. 11:3683-3693(1999) [PubMed: 10564375] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"Functional regions of the presynaptic cytomatrix protein bassoon: significance for synaptic targeting and cytomatrix anchoring."
Dresbach T., Hempelmann A., Spilker C., tom Dieck S., Altrock W.D., Zuschratter W., Garner C.C., Gundelfinger E.D.
Mol. Cell. Neurosci. 23:279-291(2003) [PubMed: 12812759] [Abstract]
Cited for: SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2.
[4]"Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and Munc13-1."
Ohtsuka T., Takao-Rikitsu E., Inoue E., Inoue M., Takeuchi M., Matsubara K., Deguchi-Tawarada M., Satoh K., Morimoto K., Nakanishi H., Takai Y.
J. Cell Biol. 158:577-590(2002) [PubMed: 12163476] [Abstract]
Cited for: INTERACTION WITH ERC2; RIMS1 AND UNC13A, DEVELOPMENTAL STAGE.
[5]"Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release."
Takao-Rikitsu E., Mochida S., Inoue E., Deguchi-Tawarada M., Inoue M., Ohtsuka T., Takai Y.
J. Cell Biol. 164:301-311(2004) [PubMed: 14734538] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ERC2; RIMS1 AND UNC13A, IDENTIFICATION IN A COMPLEX WITH RIMS1 AND ERC2.
[6]"Exploring the O-GlcNAc proteome: direct identification of O-GlcNAc-modified proteins from the brain."
Khidekel N., Ficarro S.B., Peters E.C., Hsieh-Wilson L.C.
Proc. Natl. Acad. Sci. U.S.A. 101:13132-13137(2004) [PubMed: 15340146] [Abstract]
Cited for: GLYCOSYLATION AT THR-1339.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y16563 mRNA. Translation: CAA76287.1.
IPIIPI00212553.
PIRT42761.
UniGeneRn.29999.

3D structure databases

ProteinModelPortalO88778.
ModBaseSearch...

Protein-protein interaction databases

IntActO88778. 1 interaction.
MINTMINT-4508415.
STRINGO88778.

PTM databases

PhosphoSiteO88778.

Proteomic databases

PRIDEO88778.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD2223. Bsn.

Phylogenomic databases

eggNOGroNOG04262.
GeneTreeENSGT00600000084529.
HOVERGENHBG080934.
InParanoidO88778.
OrthoDBEOG4G4GPH.

Gene expression databases

ArrayExpressO88778.
GenevestigatorO88778.
GermOnlineENSRNOG00000030714. Rattus norvegicus.

Family and domain databases

InterProIPR011011. Znf_FYVE_PHD.
IPR008899. Znf_piccolo.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
Gene3DG3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.
PfamPF05715. zf-piccolo. 2 hits.
[Graphical view]
SUPFAMSSF57903. FYVE_PHD_ZnF. 2 hits.
ProtoNetSearch...

Entry information

Entry nameBSN_RAT
AccessionPrimary (citable) accession number: O88778
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: September 21, 2011
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info