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O88766

- MMP8_RAT

UniProt

O88766 - MMP8_RAT

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Protein

Neutrophil collagenase

Gene

Mmp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Can degrade fibrillar type I, II, and III collagens.

Catalytic activityi

Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 3 Ca(2+) ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit.By similarity

Enzyme regulationi

Cannot be activated without removal of the activation peptide.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Zinc 2; in inhibited formBy similarity
Metal bindingi158 – 1581Calcium 1By similarity
Metal bindingi168 – 1681Zinc 1By similarity
Metal bindingi170 – 1701Zinc 1By similarity
Metal bindingi175 – 1751Calcium 2By similarity
Metal bindingi176 – 1761Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi178 – 1781Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi180 – 1801Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi183 – 1831Zinc 1By similarity
Metal bindingi190 – 1901Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi192 – 1921Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi194 – 1941Calcium 1By similarity
Metal bindingi196 – 1961Zinc 1By similarity
Metal bindingi198 – 1981Calcium 2By similarity
Metal bindingi201 – 2011Calcium 2By similarity
Metal bindingi218 – 2181Zinc 2; catalyticBy similarity
Active sitei219 – 2191PROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc 2; catalyticBy similarity
Metal bindingi228 – 2281Zinc 2; catalyticBy similarity
Metal bindingi287 – 2871Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi379 – 3791Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi426 – 4261Calcium 3; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: RGD
  2. metalloendopeptidase activity Source: InterPro
  3. metallopeptidase activity Source: RGD
  4. serine-type endopeptidase activity Source: UniProtKB
  5. zinc ion binding Source: RGD

GO - Biological processi

  1. collagen catabolic process Source: UniProtKB-KW
  2. ossification Source: RGD
  3. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil collagenase (EC:3.4.24.34)
Alternative name(s):
Matrix metalloproteinase-8
Short name:
MMP-8
Gene namesi
Name:Mmp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi631408. Mmp8.

Subcellular locationi

Cytoplasmic granule. Secretedextracellular spaceextracellular matrix By similarity
Note: Stored in intracellular granules.

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Propeptidei21 – 10181Activation peptideBy similarityPRO_0000028748Add
BLAST
Chaini102 – 466365Neutrophil collagenasePRO_0000028749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi280 ↔ 465Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO88766.
PRIDEiO88766.

Expressioni

Gene expression databases

GenevestigatoriO88766.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013936.

Structurei

3D structure databases

ProteinModelPortaliO88766.
SMRiO88766. Positions 100-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati277 – 32650Hemopexin 1Add
BLAST
Repeati327 – 37347Hemopexin 2Add
BLAST
Repeati375 – 42147Hemopexin 3Add
BLAST
Repeati422 – 46544Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 978Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG258253.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiO88766.
KOiK01402.
PhylomeDBiO88766.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028709. MMP8.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF137. PTHR10201:SF137. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88766-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLHLKTLPFL FFFHTQLATA LPVPPEHLEE KNMKTAENYL RKFYHLPSNQ
60 70 80 90 100
FRSARNATMI AEKLKEMQRF FGLPETGKPD AATIEIMEKP RCGVPDSGDF
110 120 130 140 150
LLTPGSPKWT HTNLTYRIIN HTPQMSKAEV KTEIEKAFKI WSVPSTLTFT
160 170 180 190 200
ETLEGEADIN IAFVSRDHGD NSPFDGPNGI LAHAFQPGRG IGGDAHFDSE
210 220 230 240 250
ETWTQDSKNY NLFLVAAHEF GHSLGLSHST DPGALMYPNY AYREPSTYSL
260 270 280 290 300
PQDDINGIQT IYGPSDNPVQ PTGPSTPTAC DPHLRFDAAT TLRGEIYFFK
310 320 330 340 350
DKYFWRRHPQ LRTVDLNFIS LFWPFLPNGL QAAYEDFDRD LVFLFKGRQY
360 370 380 390 400
WALSAYDLQQ GYPRDISNYG FPRSVQAIDA AVSYNGKTYF FVNNQCWRYD
410 420 430 440 450
NQRRSMDPGY PTSIASVFPG INCRIDAVFQ QDSFFLFFSG PQYFAFNLVS
460
RRVTRVARSN LWLNCP
Length:466
Mass (Da):53,277
Last modified:November 1, 1998 - v1
Checksum:i8B9DE97576E76C90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007288 mRNA. Translation: CAA07432.1.
RefSeqiNP_071557.1. NM_022221.1.
UniGeneiRn.44474.

Genome annotation databases

GeneIDi63849.
KEGGirno:63849.
UCSCiRGD:631408. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007288 mRNA. Translation: CAA07432.1 .
RefSeqi NP_071557.1. NM_022221.1.
UniGenei Rn.44474.

3D structure databases

ProteinModelPortali O88766.
SMRi O88766. Positions 100-263.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000013936.

Chemistry

BindingDBi O88766.
ChEMBLi CHEMBL2312.

Protein family/group databases

MEROPSi M10.002.

Proteomic databases

PaxDbi O88766.
PRIDEi O88766.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 63849.
KEGGi rno:63849.
UCSCi RGD:631408. rat.

Organism-specific databases

CTDi 4317.
RGDi 631408. Mmp8.

Phylogenomic databases

eggNOGi NOG258253.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi O88766.
KOi K01402.
PhylomeDBi O88766.

Miscellaneous databases

NextBioi 612456.
PROi O88766.

Gene expression databases

Genevestigatori O88766.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028709. MMP8.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF137. PTHR10201:SF137. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, characterization and activation properties of rat neutrophil collagenase (MMP-8)."
    Overall C.M., Lowne D., Wells G., Burel S., Clements J.M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Lewis.

Entry informationi

Entry nameiMMP8_RAT
AccessioniPrimary (citable) accession number: O88766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: November 26, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3