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O88766

- MMP8_RAT

UniProt

O88766 - MMP8_RAT

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Protein

Neutrophil collagenase

Gene
Mmp8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Can degrade fibrillar type I, II, and III collagens.

Catalytic activityi

Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.

Cofactori

Binds 3 calcium ions per subunit By similarity.
Binds 2 zinc ions per subunit By similarity.

Enzyme regulationi

Cannot be activated without removal of the activation peptide By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Zinc 2; in inhibited form By similarity
Metal bindingi158 – 1581Calcium 1 By similarity
Metal bindingi168 – 1681Zinc 1 By similarity
Metal bindingi170 – 1701Zinc 1 By similarity
Metal bindingi175 – 1751Calcium 2 By similarity
Metal bindingi176 – 1761Calcium 2; via carbonyl oxygen By similarity
Metal bindingi178 – 1781Calcium 2; via carbonyl oxygen By similarity
Metal bindingi180 – 1801Calcium 2; via carbonyl oxygen By similarity
Metal bindingi183 – 1831Zinc 1 By similarity
Metal bindingi190 – 1901Calcium 1; via carbonyl oxygen By similarity
Metal bindingi192 – 1921Calcium 1; via carbonyl oxygen By similarity
Metal bindingi194 – 1941Calcium 1 By similarity
Metal bindingi196 – 1961Zinc 1 By similarity
Metal bindingi198 – 1981Calcium 2 By similarity
Metal bindingi201 – 2011Calcium 2 By similarity
Metal bindingi218 – 2181Zinc 2; catalytic By similarity
Active sitei219 – 2191 By similarity
Metal bindingi222 – 2221Zinc 2; catalytic By similarity
Metal bindingi228 – 2281Zinc 2; catalytic By similarity
Metal bindingi287 – 2871Calcium 3; via carbonyl oxygen By similarity
Metal bindingi379 – 3791Calcium 3; via carbonyl oxygen By similarity
Metal bindingi426 – 4261Calcium 3; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. calcium ion binding Source: RGD
  2. metalloendopeptidase activity Source: InterPro
  3. metallopeptidase activity Source: RGD
  4. serine-type endopeptidase activity Source: UniProtKB
  5. zinc ion binding Source: RGD

GO - Biological processi

  1. collagen catabolic process Source: UniProtKB-KW
  2. ossification Source: RGD
  3. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil collagenase (EC:3.4.24.34)
Alternative name(s):
Matrix metalloproteinase-8
Short name:
MMP-8
Gene namesi
Name:Mmp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi631408. Mmp8.

Subcellular locationi

Cytoplasmic granule. Secretedextracellular spaceextracellular matrix By similarity
Note: Stored in intracellular granules.

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 By similarityAdd
BLAST
Propeptidei21 – 10181Activation peptide By similarityPRO_0000028748Add
BLAST
Chaini102 – 466365Neutrophil collagenasePRO_0000028749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi280 ↔ 465 Inferred

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO88766.
PRIDEiO88766.

Expressioni

Gene expression databases

GenevestigatoriO88766.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013936.

Structurei

3D structure databases

ProteinModelPortaliO88766.
SMRiO88766. Positions 100-263.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati277 – 32650Hemopexin 1Add
BLAST
Repeati327 – 37347Hemopexin 2Add
BLAST
Repeati375 – 42147Hemopexin 3Add
BLAST
Repeati422 – 46544Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 978Cysteine switch By similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.
Contains 4 hemopexin repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG258253.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiO88766.
KOiK01402.
PhylomeDBiO88766.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028709. MMP8.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF137. PTHR10201:SF137. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88766-1 [UniParc]FASTAAdd to Basket

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MLHLKTLPFL FFFHTQLATA LPVPPEHLEE KNMKTAENYL RKFYHLPSNQ    50
FRSARNATMI AEKLKEMQRF FGLPETGKPD AATIEIMEKP RCGVPDSGDF 100
LLTPGSPKWT HTNLTYRIIN HTPQMSKAEV KTEIEKAFKI WSVPSTLTFT 150
ETLEGEADIN IAFVSRDHGD NSPFDGPNGI LAHAFQPGRG IGGDAHFDSE 200
ETWTQDSKNY NLFLVAAHEF GHSLGLSHST DPGALMYPNY AYREPSTYSL 250
PQDDINGIQT IYGPSDNPVQ PTGPSTPTAC DPHLRFDAAT TLRGEIYFFK 300
DKYFWRRHPQ LRTVDLNFIS LFWPFLPNGL QAAYEDFDRD LVFLFKGRQY 350
WALSAYDLQQ GYPRDISNYG FPRSVQAIDA AVSYNGKTYF FVNNQCWRYD 400
NQRRSMDPGY PTSIASVFPG INCRIDAVFQ QDSFFLFFSG PQYFAFNLVS 450
RRVTRVARSN LWLNCP 466
Length:466
Mass (Da):53,277
Last modified:November 1, 1998 - v1
Checksum:i8B9DE97576E76C90
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007288 mRNA. Translation: CAA07432.1.
RefSeqiNP_071557.1. NM_022221.1.
UniGeneiRn.44474.

Genome annotation databases

GeneIDi63849.
KEGGirno:63849.
UCSCiRGD:631408. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ007288 mRNA. Translation: CAA07432.1 .
RefSeqi NP_071557.1. NM_022221.1.
UniGenei Rn.44474.

3D structure databases

ProteinModelPortali O88766.
SMRi O88766. Positions 100-263.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000013936.

Chemistry

BindingDBi O88766.
ChEMBLi CHEMBL2312.

Protein family/group databases

MEROPSi M10.002.

Proteomic databases

PaxDbi O88766.
PRIDEi O88766.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 63849.
KEGGi rno:63849.
UCSCi RGD:631408. rat.

Organism-specific databases

CTDi 4317.
RGDi 631408. Mmp8.

Phylogenomic databases

eggNOGi NOG258253.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi O88766.
KOi K01402.
PhylomeDBi O88766.

Miscellaneous databases

NextBioi 612456.
PROi O88766.

Gene expression databases

Genevestigatori O88766.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028709. MMP8.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF137. PTHR10201:SF137. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, characterization and activation properties of rat neutrophil collagenase (MMP-8)."
    Overall C.M., Lowne D., Wells G., Burel S., Clements J.M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Lewis.

Entry informationi

Entry nameiMMP8_RAT
AccessioniPrimary (citable) accession number: O88766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi