O88763 (PK3C3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase catalytic subunit type 3
Short name=PI3-kinase type 3
Short name=PI3K type 3
Short name=PtdIns-3-kinase type 3
EC=2.7.1.137

Alternative name(s):
Phosphoinositide-3-kinase class 3

Gene names

Name:	Pik3c3
Synonyms:	Vps34

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes By similarity. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Cofactor	Manganese By similarity.
Subunit structure	Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Interacts with AMBRA1 By similarity. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive By similarity. Part of a complex composed of PIK3R4 and PIK3CB By similarity. Interacts with RAB7A in the presence of PIK3R4 By similarity.
Subcellular location	Midbody By similarity. Late endosome By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Biological process	Autophagy Cell cycle Cell division
Cellular component	Endosome
Ligand	ATP-binding Manganese Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from mutant phenotype PubMed 12453151. Source: RGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB early endosome to late endosome transport Inferred from sequence or structural similarity. Source: UniProtKB endosome organization Inferred from mutant phenotype PubMed 12453151. Source: RGD phosphatidylinositol-mediated signaling Inferred from direct assay PubMed 12244116. Source: RGD protein processing Inferred from mutant phenotype Ref.1. Source: RGD regulation of protein secretion Inferred from mutant phenotype Ref.1. Source: RGD response to leucine Inferred from expression pattern PubMed 19015198. Source: RGD
Cellular_component	late endosome Inferred from sequence or structural similarity. Source: UniProtKB midbody Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol 3-kinase complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from direct assay PubMed 12244116. Source: RGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Phosphatidylinositol 3-kinase catalytic subunit type 3		PRO_0000088805
Regions
	Domain	35 – 184	150	C2 PI3K-type
	Domain	283 – 520	238	PIK helical
	Domain	631 – 885	255	PI3K/PI4K
Amino acid modifications
	Modified residue	261	1	Phosphoserine By similarity
	Modified residue	525	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88763 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 73822786C889035A	FASTA	887	101,534
	10 20 30 40 50 60 MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY QETCSDLYVT 70 80 90 100 110 120 CQVFAEGKPL ALPVRTSYKP FSTRWNWNEW LKLPVKYPDL PRNAQVALTI WDVYGPGRAV 130 140 150 160 170 180 PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE ADGSEPTRTP GRTSSTLSED QMSRLAKLTK 190 200 210 220 230 240 AHRQGHMVKV DWLDRLTFRE IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG 250 260 270 280 290 300 DESSPILTSF ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA TTRDQLNIIV 310 320 330 340 350 360 SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE LLGKWKPMDV 370 380 390 400 410 420 EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV QALKYENFDD IKNGLEPTKK 430 440 450 460 470 480 DSQASVSESL SSSGVSSADI DSSQIITNPL PPVASPPPAS KSKEVSDGEN LEQDLCTFLI 490 500 510 520 530 540 SRACKNSTLA NYLYWYVIVE CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS 550 560 570 580 590 600 LLAAQQTFVD RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK 610 620 630 640 650 660 IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI ISLMDKLLRK 670 680 690 700 710 720 ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI QNFFRKYAPS ETGPNGISAE 730 740 750 760 770 780 VMDTYVKSCA GYCVITYILG VGDRHLDNLL LTKTGKLFHI DFGYILGRDP KPLPPPMKLN 790 800 810 820 830 840 KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK 850 860 870 880 KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation." Row P.E., Reaves B.J., Domin J., Luzio J.P., Davidson H.W. Biochem. J. 353:655-661(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006710 mRNA. Translation: CAA07199.1. BC061981 mRNA. Translation: AAH61981.1.
IPI	IPI00212514.
RefSeq	NP_075247.1. NM_022958.2.
UniGene	Rn.30010.
3D structure databases
ProteinModelPortal	O88763.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000060791.
PTM databases
PhosphoSite	O88763.
Proteomic databases
PaxDb	O88763.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000066816; ENSRNOP00000060791; ENSRNOG00000017840.
GeneID	65052.
KEGG	rno:65052.
UCSC	RGD:620899. rat.
Organism-specific databases
CTD	5289.
RGD	620899. Pik3c3.
Phylogenomic databases
eggNOG	COG5032.
GeneTree	ENSGT00620000087805.
HOGENOM	HOG000174003.
HOVERGEN	HBG082145.
InParanoid	O88763.
KO	K00914.
OrthoDB	EOG46T30T.
Gene expression databases
ArrayExpress	O88763.
Genevestigator	O88763.
GermOnline	ENSRNOG00000017840. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 2 hits.
InterPro	IPR016024. ARM-type_fold. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00239. C2. 1 hit. SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613903.

Entry information

Entry name

PK3C3_RAT

Accession

Primary (citable) accession number: O88763

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program