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O88763

- PK3C3_RAT

UniProt

O88763 - PK3C3_RAT

Protein

Phosphatidylinositol 3-kinase catalytic subunit type 3

Gene

Pik3c3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes By similarity.By similarity

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    Cofactori

    Manganese.By similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: RGD
    2. ATP binding Source: UniProtKB-KW
    3. phosphatidylinositol 3-kinase activity Source: RGD
    4. protein binding Source: RGD
    5. protein kinase activity Source: Ensembl

    GO - Biological processi

    1. autophagic vacuole assembly Source: RGD
    2. cytokinesis Source: UniProtKB
    3. early endosome to late endosome transport Source: UniProtKB
    4. endosome organization Source: RGD
    5. phosphatidylinositol-mediated signaling Source: RGD
    6. protein processing Source: RGD
    7. regulation of protein secretion Source: RGD
    8. response to leucine Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Autophagy, Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Manganese, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198599. Synthesis of PIPs at the late endosome membrane.
    REACT_198639. Synthesis of PIPs at the Golgi membrane.
    REACT_198641. Synthesis of PIPs at the early endosome membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137)
    Short name:
    PI3-kinase type 3
    Short name:
    PI3K type 3
    Short name:
    PtdIns-3-kinase type 3
    Alternative name(s):
    Phosphoinositide-3-kinase class 3
    Gene namesi
    Name:Pik3c3
    Synonyms:Vps34
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 18

    Organism-specific databases

    RGDi620899. Pik3c3.

    Subcellular locationi

    Midbody By similarity. Late endosome By similarity
    Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.By similarity

    GO - Cellular componenti

    1. axoneme Source: UniProtKB
    2. late endosome Source: UniProtKB
    3. midbody Source: UniProtKB
    4. phosphatidylinositol 3-kinase complex Source: RefGenome

    Keywords - Cellular componenti

    Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 887887Phosphatidylinositol 3-kinase catalytic subunit type 3PRO_0000088805Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei261 – 2611PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO88763.
    PRIDEiO88763.

    PTM databases

    PhosphoSiteiO88763.

    Expressioni

    Gene expression databases

    GenevestigatoriO88763.

    Interactioni

    Subunit structurei

    Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Interacts with AMBRA1 By similarity. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive By similarity. Part of a complex composed of PIK3R4 and PIK3CB By similarity. Interacts with RAB7A in the presence of PIK3R4. Interacts with BECN1P1/BECN2 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-60851N.
    STRINGi10116.ENSRNOP00000060791.

    Structurei

    3D structure databases

    ProteinModelPortaliO88763.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 184150C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini283 – 520238PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini631 – 885255PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    GeneTreeiENSGT00620000087805.
    HOGENOMiHOG000174003.
    HOVERGENiHBG082145.
    InParanoidiO88763.
    KOiK00914.
    OMAiYLMVEFP.
    OrthoDBiEOG7PP55Z.
    PhylomeDBiO88763.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 2 hits.
    2.60.40.150. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
    SMARTiSM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88763-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY    50
    QETCSDLYVT CQVFAEGKPL ALPVRTSYKP FSTRWNWNEW LKLPVKYPDL 100
    PRNAQVALTI WDVYGPGRAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE 150
    ADGSEPTRTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE 200
    IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTSF 250
    ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA TTRDQLNIIV 300
    SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE 350
    LLGKWKPMDV EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV 400
    QALKYENFDD IKNGLEPTKK DSQASVSESL SSSGVSSADI DSSQIITNPL 450
    PPVASPPPAS KSKEVSDGEN LEQDLCTFLI SRACKNSTLA NYLYWYVIVE 500
    CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD 550
    RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK 600
    IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI 650
    ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI 700
    QNFFRKYAPS ETGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL 750
    LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK 800
    QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL 850
    SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK 887
    Length:887
    Mass (Da):101,534
    Last modified:November 1, 1998 - v1
    Checksum:i73822786C889035A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006710 mRNA. Translation: CAA07199.1.
    BC061981 mRNA. Translation: AAH61981.1.
    RefSeqiNP_075247.1. NM_022958.2.
    UniGeneiRn.30010.

    Genome annotation databases

    EnsembliENSRNOT00000066816; ENSRNOP00000060791; ENSRNOG00000017840.
    GeneIDi65052.
    KEGGirno:65052.
    UCSCiRGD:620899. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006710 mRNA. Translation: CAA07199.1 .
    BC061981 mRNA. Translation: AAH61981.1 .
    RefSeqi NP_075247.1. NM_022958.2.
    UniGenei Rn.30010.

    3D structure databases

    ProteinModelPortali O88763.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60851N.
    STRINGi 10116.ENSRNOP00000060791.

    PTM databases

    PhosphoSitei O88763.

    Proteomic databases

    PaxDbi O88763.
    PRIDEi O88763.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000066816 ; ENSRNOP00000060791 ; ENSRNOG00000017840 .
    GeneIDi 65052.
    KEGGi rno:65052.
    UCSCi RGD:620899. rat.

    Organism-specific databases

    CTDi 5289.
    RGDi 620899. Pik3c3.

    Phylogenomic databases

    eggNOGi COG5032.
    GeneTreei ENSGT00620000087805.
    HOGENOMi HOG000174003.
    HOVERGENi HBG082145.
    InParanoidi O88763.
    KOi K00914.
    OMAi YLMVEFP.
    OrthoDBi EOG7PP55Z.
    PhylomeDBi O88763.

    Enzyme and pathway databases

    Reactomei REACT_198599. Synthesis of PIPs at the late endosome membrane.
    REACT_198639. Synthesis of PIPs at the Golgi membrane.
    REACT_198641. Synthesis of PIPs at the early endosome membrane.

    Miscellaneous databases

    NextBioi 613903.
    PROi O88763.

    Gene expression databases

    Genevestigatori O88763.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 2 hits.
    2.60.40.150. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
    SMARTi SM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation."
      Row P.E., Reaves B.J., Domin J., Luzio J.P., Davidson H.W.
      Biochem. J. 353:655-661(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiPK3C3_RAT
    AccessioniPrimary (citable) accession number: O88763
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3