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O88751

- CABP1_RAT

UniProt

O88751 - CABP1_RAT

Protein

Calcium-binding protein 1

Gene

Cabp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D By similarity. Inhibits TRPC5 channels By similarity. Prevents NMDA receptor-induced cellular degeneration.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi166 – 1661MagnesiumBy similarity
    Metal bindingi168 – 1681MagnesiumBy similarity
    Metal bindingi170 – 1701MagnesiumBy similarity
    Metal bindingi172 – 1721Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi243 – 2431Calcium 1By similarity
    Metal bindingi245 – 2451Calcium 1By similarity
    Metal bindingi247 – 2471Calcium 1By similarity
    Metal bindingi249 – 2491Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi254 – 2541Calcium 1By similarity
    Metal bindingi280 – 2801Calcium 2By similarity
    Metal bindingi281 – 2811Calcium 2; via amide nitrogenBy similarity
    Metal bindingi282 – 2821Calcium 2By similarity
    Metal bindingi284 – 2841Calcium 2By similarity
    Metal bindingi285 – 2851Calcium 2; via amide nitrogenBy similarity
    Metal bindingi286 – 2861Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi288 – 2881Calcium 2By similarity
    Metal bindingi291 – 2911Calcium 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi166 – 177121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi243 – 254122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi280 – 291123PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: UniProtKB
    2. calcium ion binding Source: RGD
    3. ion channel binding Source: RGD
    4. nuclear localization sequence binding Source: UniProtKB
    5. protein binding Source: UniProtKB
    6. protein domain specific binding Source: RGD
    7. protein homodimerization activity Source: RGD
    8. type 3 metabotropic glutamate receptor binding Source: RGD

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. negative regulation of cell communication by electrical coupling Source: RGD
    3. negative regulation of protein import into nucleus Source: UniProtKB
    4. negative regulation of voltage-gated calcium channel activity Source: RGD
    5. regulation of synaptic plasticity Source: RGD
    6. regulation of voltage-gated calcium channel activity Source: RGD

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-binding protein 1
    Short name:
    CaBP1
    Alternative name(s):
    Caldendrin
    Gene namesi
    Name:Cabp1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi620385. Cabp1.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Occurs in both the cytoplasmic and cytoskeletal compartment of cell somata and dendrites.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytoplasmic side of plasma membrane Source: RGD
    3. cytoskeleton Source: RGD
    4. cytosol Source: RGD
    5. dendrite Source: UniProtKB
    6. Golgi membrane Source: Ensembl
    7. neuronal cell body Source: UniProtKB
    8. nucleus Source: UniProtKB
    9. perinuclear region of cytoplasm Source: RGD
    10. plasma membrane Source: RGD
    11. postsynaptic density Source: UniProtKB
    12. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Calcium-binding protein 1PRO_0000073515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei251 – 2511PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated. The phosphorylation regulates the activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO88751.
    PRIDEiO88751.

    Expressioni

    Tissue specificityi

    Somatodendritic compartment of neurons. Restricted expression in retina to a subpopulation of amacrine, bipolar, and ganglion cells. According to PubMed:11906216, expression is heterogeneous within brain regions and their major cell types and does not match with those of marker proteins for characterized neuronal subpopulations.1 Publication

    Developmental stagei

    Its expression is regulated differentially in retinal cell types during development.

    Gene expression databases

    ArrayExpressiO88751.
    GenevestigatoriO88751.

    Interactioni

    Subunit structurei

    Interacts ITPR1, ITPR2 and ITPR3. The strength of this interaction inversely correlates with calcium concentration. Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes. Interacts with CACNA1C. Interacts with CACNA1D By similarity. Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B. Interacts (via EF-hands 1 and 2) with NSMF (via the central NLS-containing motif region), the interaction occurs in a calcium dependent manner after synaptic NMDA receptor stimulation and prevents nuclear import of NSMF. Interacts with MYO1C and TRPC5 By similarity.By similarity

    Protein-protein interaction databases

    IntActiO88751. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO88751.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 18836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini189 – 22436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini230 – 26536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini267 – 29832EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.By similarity

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    GeneTreeiENSGT00740000114946.
    HOGENOMiHOG000233018.
    HOVERGENiHBG012180.
    InParanoidiO88751.
    OMAiVLAQNCA.
    OrthoDBiEOG7XM303.
    PhylomeDBiO88751.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF00036. EF-hand_1. 3 hits.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O88751-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSHIAKSES KTSLLKAAAA SGGSRAPRHS SARDPGLRGR RLPGPCPDSP    50
    ATCGDPSSRR PLCRPVPRDE GARGSRRGLP QAHCRPRETL PPARGRDGEE 100
    RGLAPALSLR GSLRSRGRGD PAPAGTPEAD PFLHQLRPML SSAFGQDRSL 150
    RPEEIEELRE AFREFDKDKD GYINCRDLGN CMRTMGYMPT EMELIELSQQ 200
    INMNLGGHVD FDDFVELMGP KLLAETADMI GVKELRDAFR EFDTNGDGEI 250
    STSELREAMR KLLGHQVGHR DIEEIIRDVD LNGDGRVDFE EFVRMMSR 298

    Note: Major isoform expressed in the brain.

    Length:298
    Mass (Da):33,017
    Last modified:May 27, 2002 - v2
    Checksum:i8A9B74CD6B9F6875
    GO
    Isoform 2 (identifier: O88751-2) [UniParc]FASTAAdd to Basket

    Also known as: Caldendrin-S1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLRNLSRK

    Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.

    Show »
    Length:167
    Mass (Da):19,354
    Checksum:i3C65D1F2C4338AF3
    GO
    Isoform 3 (identifier: O88751-3) [UniParc]FASTAAdd to Basket

    Also known as: Caldendrin-S2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLR...RKGFAENRQP

    Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.

    Show »
    Length:227
    Mass (Da):25,903
    Checksum:iC0BCC66C8FF0C88F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 146146MSSHI…SAFGQ → MGNCVKSPLRNLSRK in isoform 2. 1 PublicationVSP_026157Add
    BLAST
    Alternative sequencei1 – 146146MSSHI…SAFGQ → MGNCVKSPLRNLSRKMRQEE KTSYMAVQTSEDGLADGGEL PGPLMMLAQNCAVMHNLLGP ACIFLRKGFAENRQP in isoform 3. 1 PublicationVSP_026158Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17048 mRNA. Translation: CAD20347.1.
    AJ315761 mRNA. Translation: CAC43037.1.
    AJ315657 mRNA. Translation: CAC42417.1.
    RefSeqiNP_001028847.1. NM_001033675.1. [O88751-2]
    NP_001028848.1. NM_001033676.1.
    NP_598213.1. NM_133529.2. [O88751-3]
    UniGeneiRn.23560.

    Genome annotation databases

    EnsembliENSRNOT00000001551; ENSRNOP00000001551; ENSRNOG00000001173. [O88751-1]
    ENSRNOT00000001552; ENSRNOP00000001552; ENSRNOG00000001173. [O88751-3]
    ENSRNOT00000039281; ENSRNOP00000033685; ENSRNOG00000001173. [O88751-2]
    GeneIDi171051.
    KEGGirno:171051.
    UCSCiRGD:620385. rat. [O88751-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17048 mRNA. Translation: CAD20347.1 .
    AJ315761 mRNA. Translation: CAC43037.1 .
    AJ315657 mRNA. Translation: CAC42417.1 .
    RefSeqi NP_001028847.1. NM_001033675.1. [O88751-2 ]
    NP_001028848.1. NM_001033676.1.
    NP_598213.1. NM_133529.2. [O88751-3 ]
    UniGenei Rn.23560.

    3D structure databases

    ProteinModelPortali O88751.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O88751. 1 interaction.

    Proteomic databases

    PaxDbi O88751.
    PRIDEi O88751.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001551 ; ENSRNOP00000001551 ; ENSRNOG00000001173 . [O88751-1 ]
    ENSRNOT00000001552 ; ENSRNOP00000001552 ; ENSRNOG00000001173 . [O88751-3 ]
    ENSRNOT00000039281 ; ENSRNOP00000033685 ; ENSRNOG00000001173 . [O88751-2 ]
    GeneIDi 171051.
    KEGGi rno:171051.
    UCSCi RGD:620385. rat. [O88751-1 ]

    Organism-specific databases

    CTDi 9478.
    RGDi 620385. Cabp1.

    Phylogenomic databases

    eggNOGi COG5126.
    GeneTreei ENSGT00740000114946.
    HOGENOMi HOG000233018.
    HOVERGENi HBG012180.
    InParanoidi O88751.
    OMAi VLAQNCA.
    OrthoDBi EOG7XM303.
    PhylomeDBi O88751.

    Miscellaneous databases

    NextBioi 621595.

    Gene expression databases

    ArrayExpressi O88751.
    Genevestigatori O88751.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF00036. EF-hand_1. 3 hits.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Caldendrin, a novel neuronal calcium-binding protein confined to the somato-dendritic compartment."
      Seidenbecher C.I., Langnaese K., Sanmarti-Vila L., Boeckers T.M., Smalla K.-H., Sabel B.A., Garner C.C., Gundelfinger E.D., Kreutz M.R.
      J. Biol. Chem. 273:21324-21331(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION.
      Strain: Sprague-Dawley.
    2. "The neuron-specific Ca2+-binding protein caldendrin: gene structure, splice isoforms, and expression in the rat central nervous system."
      Laube G., Seidenbecher C.I., Richter K., Dieterich D.C., Hoffmann B., Landwehr M., Smalla K.H., Winter C., Boeckers T.M., Wolf G., Gundelfinger E.D., Kreutz M.R.
      Mol. Cell. Neurosci. 19:459-475(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY.
      Tissue: Brain.
    3. Seidenbecher C.I.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 290-291.
    4. "Identification of a family of calcium sensors as protein ligands of inositol trisphosphate receptor Ca(2+) release channels."
      Yang J., McBride S., Mak D.-O.D., Vardi N., Palczewski K., Haeseleer F., Foskett J.K.
      Proc. Natl. Acad. Sci. U.S.A. 99:7711-7716(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ITPR1; ITPR2 AND ITPR3.
    5. "Differential modulation of Ca(v)2.1 channels by calmodulin and Ca2+-binding protein 1."
      Lee A., Westenbroek R.E., Haeseleer F., Palczewski K., Scheuer T., Catterall W.A.
      Nat. Neurosci. 5:210-217(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CACNA1A.
    6. "Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins."
      Seidenbecher C.I., Landwehr M., Smalla K.H., Kreutz M., Dieterich D.C., Zuschratter W., Reissner C., Hammarback J.A., Bockers T.M., Gundelfinger E.D., Kreutz M.R.
      J. Mol. Biol. 336:957-970(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAP1LC3B, SUBCELLULAR LOCATION.
    7. Cited for: INTERACTION WITH NSMF.

    Entry informationi

    Entry nameiCABP1_RAT
    AccessioniPrimary (citable) accession number: O88751
    Secondary accession number(s): Q711K8, Q91WZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3