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Protein

Calcium-binding protein 1

Gene

Cabp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D (By similarity). Inhibits TRPC5 channels (By similarity). Prevents NMDA receptor-induced cellular degeneration.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi166MagnesiumBy similarity1
Metal bindingi168MagnesiumBy similarity1
Metal bindingi170MagnesiumBy similarity1
Metal bindingi172Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi243Calcium 1By similarity1
Metal bindingi245Calcium 1By similarity1
Metal bindingi247Calcium 1By similarity1
Metal bindingi249Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi254Calcium 1By similarity1
Metal bindingi280Calcium 2By similarity1
Metal bindingi281Calcium 2; via amide nitrogenBy similarity1
Metal bindingi282Calcium 2By similarity1
Metal bindingi284Calcium 2By similarity1
Metal bindingi285Calcium 2; via amide nitrogenBy similarity1
Metal bindingi286Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi288Calcium 2By similarity1
Metal bindingi291Calcium 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi166 – 1771PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi243 – 2542PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi280 – 2913PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • calcium ion binding Source: RGD
  • ion channel binding Source: RGD
  • nuclear localization sequence binding Source: UniProtKB
  • protein domain specific binding Source: RGD
  • protein homodimerization activity Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of cell communication by electrical coupling Source: RGD
  • negative regulation of protein import into nucleus Source: UniProtKB
  • negative regulation of voltage-gated calcium channel activity Source: RGD
  • regulation of synaptic plasticity Source: RGD
  • regulation of voltage-gated calcium channel activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 1
Short name:
CaBP1
Alternative name(s):
Caldendrin
Gene namesi
Name:Cabp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620385. Cabp1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic side of plasma membrane Source: RGD
  • cytoskeleton Source: RGD
  • cytosol Source: RGD
  • dendrite Source: UniProtKB
  • neuronal cell body Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: UniProtKB
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000735151 – 298Calcium-binding protein 1Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineBy similarity1
Isoform 2 (identifier: O88751-2)
Lipidationi2N-myristoyl glycine1
Isoform 3 (identifier: O88751-3)
Lipidationi2N-myristoyl glycine1

Post-translational modificationi

Phosphorylated. The phosphorylation regulates the activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88751.
PRIDEiO88751.

Expressioni

Tissue specificityi

Somatodendritic compartment of neurons. Restricted expression in retina to a subpopulation of amacrine, bipolar, and ganglion cells. According to PubMed:11906216, expression is heterogeneous within brain regions and their major cell types and does not match with those of marker proteins for characterized neuronal subpopulations.1 Publication

Developmental stagei

Its expression is regulated differentially in retinal cell types during development.

Gene expression databases

BgeeiENSRNOG00000001173.
ExpressionAtlasiO88751. baseline and differential.
GenevisibleiO88751. RN.

Interactioni

Subunit structurei

Interacts ITPR1, ITPR2 and ITPR3. The strength of this interaction inversely correlates with calcium concentration. Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes. Interacts with CACNA1C. Interacts with CACNA1D (By similarity). Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B. Interacts (via EF-hands 1 and 2) with NSMF (via the central NLS-containing motif region), the interaction occurs in a calcium dependent manner after synaptic NMDA receptor stimulation and prevents nuclear import of NSMF. Interacts with MYO1C and TRPC5. Interacts with SPACA9 (By similarity).By similarity3 Publications

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • ion channel binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein homodimerization activity Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

IntActiO88751. 1 interactor.
STRINGi10116.ENSRNOP00000001551.

Structurei

3D structure databases

ProteinModelPortaliO88751.
SMRiO88751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 188EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini189 – 224EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini230 – 265EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini267 – 298EF-hand 4PROSITE-ProRule annotationAdd BLAST32

Domaini

EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.By similarity

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiO88751.
OMAiVLAQNCA.
OrthoDBiEOG091G0PB4.
PhylomeDBiO88751.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88751-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSHIAKSES KTSLLKAAAA SGGSRAPRHS SARDPGLRGR RLPGPCPDSP
60 70 80 90 100
ATCGDPSSRR PLCRPVPRDE GARGSRRGLP QAHCRPRETL PPARGRDGEE
110 120 130 140 150
RGLAPALSLR GSLRSRGRGD PAPAGTPEAD PFLHQLRPML SSAFGQDRSL
160 170 180 190 200
RPEEIEELRE AFREFDKDKD GYINCRDLGN CMRTMGYMPT EMELIELSQQ
210 220 230 240 250
INMNLGGHVD FDDFVELMGP KLLAETADMI GVKELRDAFR EFDTNGDGEI
260 270 280 290
STSELREAMR KLLGHQVGHR DIEEIIRDVD LNGDGRVDFE EFVRMMSR
Note: Major isoform expressed in the brain.
Length:298
Mass (Da):33,017
Last modified:May 27, 2002 - v2
Checksum:i8A9B74CD6B9F6875
GO
Isoform 2 (identifier: O88751-2) [UniParc]FASTAAdd to basket
Also known as: Caldendrin-S1

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLRNLSRK

Note: Deamidated on Asn-3. S-palmitoylated on Cys-4. Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.
Show »
Length:167
Mass (Da):19,354
Checksum:i3C65D1F2C4338AF3
GO
Isoform 3 (identifier: O88751-3) [UniParc]FASTAAdd to basket
Also known as: Caldendrin-S2

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLR...RKGFAENRQP

Note: Deamidated on Asn-3. S-palmitoylated on Cys-4. Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.
Show »
Length:227
Mass (Da):25,903
Checksum:iC0BCC66C8FF0C88F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0261571 – 146MSSHI…SAFGQ → MGNCVKSPLRNLSRK in isoform 2. 1 PublicationAdd BLAST146
Alternative sequenceiVSP_0261581 – 146MSSHI…SAFGQ → MGNCVKSPLRNLSRKMRQEE KTSYMAVQTSEDGLADGGEL PGPLMMLAQNCAVMHNLLGP ACIFLRKGFAENRQP in isoform 3. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17048 mRNA. Translation: CAD20347.1.
AJ315761 mRNA. Translation: CAC43037.1.
AJ315657 mRNA. Translation: CAC42417.1.
RefSeqiNP_001028847.1. NM_001033675.1. [O88751-2]
NP_001028848.1. NM_001033676.1.
NP_598213.1. NM_133529.2. [O88751-3]
UniGeneiRn.23560.

Genome annotation databases

EnsembliENSRNOT00000001552; ENSRNOP00000001552; ENSRNOG00000001173. [O88751-3]
ENSRNOT00000039281; ENSRNOP00000033685; ENSRNOG00000001173. [O88751-2]
GeneIDi171051.
KEGGirno:171051.
UCSCiRGD:620385. rat. [O88751-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17048 mRNA. Translation: CAD20347.1.
AJ315761 mRNA. Translation: CAC43037.1.
AJ315657 mRNA. Translation: CAC42417.1.
RefSeqiNP_001028847.1. NM_001033675.1. [O88751-2]
NP_001028848.1. NM_001033676.1.
NP_598213.1. NM_133529.2. [O88751-3]
UniGeneiRn.23560.

3D structure databases

ProteinModelPortaliO88751.
SMRiO88751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88751. 1 interactor.
STRINGi10116.ENSRNOP00000001551.

Proteomic databases

PaxDbiO88751.
PRIDEiO88751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001552; ENSRNOP00000001552; ENSRNOG00000001173. [O88751-3]
ENSRNOT00000039281; ENSRNOP00000033685; ENSRNOG00000001173. [O88751-2]
GeneIDi171051.
KEGGirno:171051.
UCSCiRGD:620385. rat. [O88751-1]

Organism-specific databases

CTDi9478.
RGDi620385. Cabp1.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiO88751.
OMAiVLAQNCA.
OrthoDBiEOG091G0PB4.
PhylomeDBiO88751.

Miscellaneous databases

PROiO88751.

Gene expression databases

BgeeiENSRNOG00000001173.
ExpressionAtlasiO88751. baseline and differential.
GenevisibleiO88751. RN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCABP1_RAT
AccessioniPrimary (citable) accession number: O88751
Secondary accession number(s): Q711K8, Q91WZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.