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O88751

- CABP1_RAT

UniProt

O88751 - CABP1_RAT

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Protein

Calcium-binding protein 1

Gene
Cabp1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D By similarity. Inhibits TRPC5 channels By similarity. Prevents NMDA receptor-induced cellular degeneration.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi166 – 1661Magnesium By similarity
Metal bindingi168 – 1681Magnesium By similarity
Metal bindingi170 – 1701Magnesium By similarity
Metal bindingi172 – 1721Magnesium; via carbonyl oxygen By similarity
Metal bindingi243 – 2431Calcium 1 By similarity
Metal bindingi245 – 2451Calcium 1 By similarity
Metal bindingi247 – 2471Calcium 1 By similarity
Metal bindingi249 – 2491Calcium 1; via carbonyl oxygen By similarity
Metal bindingi254 – 2541Calcium 1 By similarity
Metal bindingi280 – 2801Calcium 2 By similarity
Metal bindingi281 – 2811Calcium 2; via amide nitrogen By similarity
Metal bindingi282 – 2821Calcium 2 By similarity
Metal bindingi284 – 2841Calcium 2 By similarity
Metal bindingi285 – 2851Calcium 2; via amide nitrogen By similarity
Metal bindingi286 – 2861Calcium 2; via carbonyl oxygen By similarity
Metal bindingi288 – 2881Calcium 2 By similarity
Metal bindingi291 – 2911Calcium 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi166 – 177121 Reviewed predictionAdd
BLAST
Calcium bindingi243 – 254122 Reviewed predictionAdd
BLAST
Calcium bindingi280 – 291123 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent protein binding Source: UniProtKB
  2. calcium ion binding Source: RGD
  3. ion channel binding Source: RGD
  4. nuclear localization sequence binding Source: UniProtKB
  5. protein binding Source: UniProtKB
  6. protein domain specific binding Source: RGD
  7. protein homodimerization activity Source: RGD
  8. type 3 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. negative regulation of cell communication by electrical coupling Source: RGD
  3. negative regulation of protein import into nucleus Source: UniProtKB
  4. negative regulation of voltage-gated calcium channel activity Source: RGD
  5. regulation of synaptic plasticity Source: RGD
  6. regulation of voltage-gated calcium channel activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 1
Short name:
CaBP1
Alternative name(s):
Caldendrin
Gene namesi
Name:Cabp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi620385. Cabp1.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton
Note: Occurs in both the cytoplasmic and cytoskeletal compartment of cell somata and dendrites.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoplasmic side of plasma membrane Source: RGD
  3. cytoskeleton Source: RGD
  4. cytosol Source: RGD
  5. dendrite Source: UniProtKB
  6. Golgi membrane Source: Ensembl
  7. neuronal cell body Source: UniProtKB
  8. nucleus Source: UniProtKB
  9. perinuclear region of cytoplasm Source: RGD
  10. plasma membrane Source: RGD
  11. postsynaptic density Source: UniProtKB
  12. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Calcium-binding protein 1PRO_0000073515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511Phosphoserine By similarity

Post-translational modificationi

Phosphorylated. The phosphorylation regulates the activity By similarity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88751.
PRIDEiO88751.

Expressioni

Tissue specificityi

Somatodendritic compartment of neurons. Restricted expression in retina to a subpopulation of amacrine, bipolar, and ganglion cells. According to 1 Publication, expression is heterogeneous within brain regions and their major cell types and does not match with those of marker proteins for characterized neuronal subpopulations.1 Publication

Developmental stagei

Its expression is regulated differentially in retinal cell types during development.

Gene expression databases

ArrayExpressiO88751.
GenevestigatoriO88751.

Interactioni

Subunit structurei

Interacts ITPR1, ITPR2 and ITPR3. The strength of this interaction inversely correlates with calcium concentration. Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes. Interacts with CACNA1C. Interacts with CACNA1D By similarity. Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B. Interacts (via EF-hands 1 and 2) with NSMF (via the central NLS-containing motif region), the interaction occurs in a calcium dependent manner after synaptic NMDA receptor stimulation and prevents nuclear import of NSMF. Interacts with MYO1C and TRPC5 By similarity.4 Publications

Protein-protein interaction databases

IntActiO88751. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO88751.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 18836EF-hand 1Add
BLAST
Domaini189 – 22436EF-hand 2Add
BLAST
Domaini230 – 26536EF-hand 3Add
BLAST
Domaini267 – 29832EF-hand 4Add
BLAST

Domaini

EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium By similarity.

Sequence similaritiesi

Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00740000114946.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiO88751.
OMAiVLAQNCA.
OrthoDBiEOG7XM303.
PhylomeDBiO88751.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 3 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88751-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSHIAKSES KTSLLKAAAA SGGSRAPRHS SARDPGLRGR RLPGPCPDSP    50
ATCGDPSSRR PLCRPVPRDE GARGSRRGLP QAHCRPRETL PPARGRDGEE 100
RGLAPALSLR GSLRSRGRGD PAPAGTPEAD PFLHQLRPML SSAFGQDRSL 150
RPEEIEELRE AFREFDKDKD GYINCRDLGN CMRTMGYMPT EMELIELSQQ 200
INMNLGGHVD FDDFVELMGP KLLAETADMI GVKELRDAFR EFDTNGDGEI 250
STSELREAMR KLLGHQVGHR DIEEIIRDVD LNGDGRVDFE EFVRMMSR 298

Note: Major isoform expressed in the brain.

Length:298
Mass (Da):33,017
Last modified:May 27, 2002 - v2
Checksum:i8A9B74CD6B9F6875
GO
Isoform 2 (identifier: O88751-2) [UniParc]FASTAAdd to Basket

Also known as: Caldendrin-S1

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLRNLSRK

Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.

Show »
Length:167
Mass (Da):19,354
Checksum:i3C65D1F2C4338AF3
GO
Isoform 3 (identifier: O88751-3) [UniParc]FASTAAdd to Basket

Also known as: Caldendrin-S2

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLR...RKGFAENRQP

Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.

Show »
Length:227
Mass (Da):25,903
Checksum:iC0BCC66C8FF0C88F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 146146MSSHI…SAFGQ → MGNCVKSPLRNLSRK in isoform 2. VSP_026157Add
BLAST
Alternative sequencei1 – 146146MSSHI…SAFGQ → MGNCVKSPLRNLSRKMRQEE KTSYMAVQTSEDGLADGGEL PGPLMMLAQNCAVMHNLLGP ACIFLRKGFAENRQP in isoform 3. VSP_026158Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17048 mRNA. Translation: CAD20347.1.
AJ315761 mRNA. Translation: CAC43037.1.
AJ315657 mRNA. Translation: CAC42417.1.
RefSeqiNP_001028847.1. NM_001033675.1. [O88751-2]
NP_001028848.1. NM_001033676.1.
NP_598213.1. NM_133529.2. [O88751-3]
UniGeneiRn.23560.

Genome annotation databases

EnsembliENSRNOT00000001551; ENSRNOP00000001551; ENSRNOG00000001173. [O88751-1]
ENSRNOT00000001552; ENSRNOP00000001552; ENSRNOG00000001173. [O88751-3]
ENSRNOT00000039281; ENSRNOP00000033685; ENSRNOG00000001173. [O88751-2]
GeneIDi171051.
KEGGirno:171051.
UCSCiRGD:620385. rat. [O88751-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17048 mRNA. Translation: CAD20347.1 .
AJ315761 mRNA. Translation: CAC43037.1 .
AJ315657 mRNA. Translation: CAC42417.1 .
RefSeqi NP_001028847.1. NM_001033675.1. [O88751-2 ]
NP_001028848.1. NM_001033676.1.
NP_598213.1. NM_133529.2. [O88751-3 ]
UniGenei Rn.23560.

3D structure databases

ProteinModelPortali O88751.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88751. 1 interaction.

Proteomic databases

PaxDbi O88751.
PRIDEi O88751.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001551 ; ENSRNOP00000001551 ; ENSRNOG00000001173 . [O88751-1 ]
ENSRNOT00000001552 ; ENSRNOP00000001552 ; ENSRNOG00000001173 . [O88751-3 ]
ENSRNOT00000039281 ; ENSRNOP00000033685 ; ENSRNOG00000001173 . [O88751-2 ]
GeneIDi 171051.
KEGGi rno:171051.
UCSCi RGD:620385. rat. [O88751-1 ]

Organism-specific databases

CTDi 9478.
RGDi 620385. Cabp1.

Phylogenomic databases

eggNOGi COG5126.
GeneTreei ENSGT00740000114946.
HOGENOMi HOG000233018.
HOVERGENi HBG012180.
InParanoidi O88751.
OMAi VLAQNCA.
OrthoDBi EOG7XM303.
PhylomeDBi O88751.

Miscellaneous databases

NextBioi 621595.

Gene expression databases

ArrayExpressi O88751.
Genevestigatori O88751.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF00036. EF-hand_1. 3 hits.
[Graphical view ]
SMARTi SM00054. EFh. 3 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Caldendrin, a novel neuronal calcium-binding protein confined to the somato-dendritic compartment."
    Seidenbecher C.I., Langnaese K., Sanmarti-Vila L., Boeckers T.M., Smalla K.-H., Sabel B.A., Garner C.C., Gundelfinger E.D., Kreutz M.R.
    J. Biol. Chem. 273:21324-21331(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION.
    Strain: Sprague-Dawley.
  2. "The neuron-specific Ca2+-binding protein caldendrin: gene structure, splice isoforms, and expression in the rat central nervous system."
    Laube G., Seidenbecher C.I., Richter K., Dieterich D.C., Hoffmann B., Landwehr M., Smalla K.H., Winter C., Boeckers T.M., Wolf G., Gundelfinger E.D., Kreutz M.R.
    Mol. Cell. Neurosci. 19:459-475(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY.
    Tissue: Brain.
  3. Seidenbecher C.I.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 290-291.
  4. "Identification of a family of calcium sensors as protein ligands of inositol trisphosphate receptor Ca(2+) release channels."
    Yang J., McBride S., Mak D.-O.D., Vardi N., Palczewski K., Haeseleer F., Foskett J.K.
    Proc. Natl. Acad. Sci. U.S.A. 99:7711-7716(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ITPR1; ITPR2 AND ITPR3.
  5. "Differential modulation of Ca(v)2.1 channels by calmodulin and Ca2+-binding protein 1."
    Lee A., Westenbroek R.E., Haeseleer F., Palczewski K., Scheuer T., Catterall W.A.
    Nat. Neurosci. 5:210-217(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CACNA1A.
  6. "Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins."
    Seidenbecher C.I., Landwehr M., Smalla K.H., Kreutz M., Dieterich D.C., Zuschratter W., Reissner C., Hammarback J.A., Bockers T.M., Gundelfinger E.D., Kreutz M.R.
    J. Mol. Biol. 336:957-970(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAP1LC3B, SUBCELLULAR LOCATION.
  7. Cited for: INTERACTION WITH NSMF.

Entry informationi

Entry nameiCABP1_RAT
AccessioniPrimary (citable) accession number: O88751
Secondary accession number(s): Q711K8, Q91WZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 27, 2002
Last modified: June 11, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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