Reviewed, UniProtKB/Swiss-Prot O88746 (TOM1_MOUSE)

Names and origin

Protein names

Recommended name:
    Target of Myb protein 1

Gene names

Name:

Tom1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in intracellular trafficking. Probable association with membranes.
Subunit structure	Interacts with ZFYVE16; interaction is required to target it to endosomes By similarity.
Subcellular location	Cytoplasm Probable. Membrane; Peripheral membrane protein Probable.
Tissue specificity	Ubiquitous. In adult brain, it is highly expressed at the mesencephalic level, in the hippocampal formation and medial lemniscus. In cerebellum, it is highly expressed in Purkinje cells and granular layers.
Developmental stage	Ubiquitously expressed in the embryo, with a higher expression in the intestines.
Sequence similarities	Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB early endosome Inferred from sequence or structural similarity. Source: UniProtKB extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APLP1	P51693	1	EBI-74264,EBI-74648	From a different organism.
Tollip	Q9QZ06	1	EBI-74264,EBI-74272

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O88746-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O88746-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-324: Missing.
     325-343: MGPDPAATNNLSSQLAGMN → MGRANGTAGLLPGPSVSAD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Target of Myb protein 1		PRO_0000072629
Regions
	Domain	20 – 152	133	VHS
	Domain	215 – 303	89	GAT
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	130	1	Phosphoserine By similarity
	Modified residue	131	1	Phosphoserine By similarity
	Modified residue	160	1	Phosphoserine By similarity
	Modified residue	164	1	Phosphothreonine By similarity
	Modified residue	174	1	Phosphothreonine Ref.4
	Modified residue	176	1	Phosphoserine Ref.4
	Modified residue	180	1	Phosphoserine Ref.4
	Modified residue	355	1	Phosphoserine By similarity
	Modified residue	386	1	Phosphotyrosine By similarity
	Modified residue	462	1	Phosphoserine By similarity
	Modified residue	464	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 324	324	Missing in isoform 2.		VSP_003991
	Alternative sequence	325 – 343	19	MGPDP…LAGMN → MGRANGTAGLLPGPSVSAD in isoform 2.		VSP_003992

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A15F24FD9B4D31C3	FASTA	492	54,325
	10 20 30 40 50 60 MDFLLGNPFS SPVGQRIEKA TDGSLQSEDW ALNMEICDII NETEEGPKDA FRAVKKRIMG 70 80 90 100 110 120 NKNFHEVMLA LTVLETCVKN CGHRFHVLVA NQDFVENVLV RTILPKNNPP TIVHDKVLNL 130 140 150 160 170 180 IQSWADAFRS SPDLTGVVAV YEDLRRKGLE FPMTDLDMLS PIHTPQRTVF NSETPSRQNS 190 200 210 220 230 240 VSSNTSQRGD LSQHATPLPT PAVLPGDSPI TPTPEQIGKL RSELEMVSGN VRVMSEMLTE 250 260 270 280 290 300 LVPTQVEPAD LELLQELNRT CRAMQQRILE LIPRISNEQL TEELLMINDN LNNVFLRHER 310 320 330 340 350 360 FERFRTGQTA KASSEAELAT DLIDMGPDPA ATNNLSSQLA GMNLGSRSVR AGLQSLETSG 370 380 390 400 410 420 HLEDDFDMFA LTRGSSLADQ RKGVKYEAPQ TTDGLAGALD ARQQSTGAIP ATQARIMEDI 430 440 450 460 470 480 EQWLSTDVGN SAEEPSGVTS EEFDKFLEER AKAADRLPNL ASPSAEGPPR PSPGTAPRRK 490 TQEKDDDMLF AL
	Isoform 2. Checksum: 3AA2CF5B2EBAD575	FASTA	168	17,788
	10 20 30 40 50 60 MGRANGTAGL LPGPSVSADL GSRSVRAGLQ SLETSGHLED DFDMFALTRG SSLADQRKGV 70 80 90 100 110 120 KYEAPQTTDG LAGALDARQQ STGAIPATQA RIMEDIEQWL STDVGNSAEE PSGVTSEEFD 130 140 150 160 KFLEERAKAA DRLPNLASPS AEGPPRPSPG TAPRRKTQEK DDDMLFAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM." Seroussi E., Kedra D., Kost-Alimova M., Sandberg-Nordqvist A.-C., Fransson I., Jacobs J.F.M., Fu Y., Pan H.-Q., Roe B.A., Imreh S., Dumanski J.P. Genomics 57:380-388(1999) [PubMed: 10329004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-174; SER-176 AND SER-180, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006972 mRNA. Translation: CAA07361.1. AK004063 mRNA. Translation: BAE43160.1. AK028398 mRNA. Translation: BAC25932.1. BC021633 mRNA. Translation: AAH21633.1.
IPI	IPI00222197. IPI00380814.
RefSeq	NP_035752.1. XP_620367.1.
UniGene	Mm.1967 Mm.290868
3D structure databases
SMR	O88746. Positions 2-153, 215-307.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88746. 3 interactions.
PTM databases
PhosphoSite	O88746.
Proteomic databases
PRIDE	O88746.
Genome annotation databases
Ensembl	ENSMUST00000036712; ENSMUSP00000036849; ENSMUSG00000037827; Mus musculus. [Genome view]
GeneID	21968. 545878.
UCSC	uc009mha.1. mouse. uc009mhb.1. mouse.
Organism-specific databases
CTD	21968.
MGI	MGI:1338026. Tom1.
Phylogenomic databases
HOGENOM	O88746.
HOVERGEN	O88746.
OMA	GAIPVTQ
OrthoDB	EOG9MKR1H
Gene expression databases
Bgee	O88746.
CleanEx	MM_TOM1.
Genevestigator	O88746.
GermOnline	ENSMUSG00000037827. Mus musculus.
Family and domain databases
InterPro	IPR008942. ENTH_VHS. IPR004152. GAT. IPR014645. TOM1. IPR002014. VHS. IPR018205. VHS_subgroup. [Graphical view]
Gene3D	G3DSA:1.25.40.90. ENTH_VHS. 1 hit.
Pfam	PF03127. GAT. 1 hit. PF00790. VHS. 1 hit. [Graphical view]
PIRSF	PIRSF036948. TOM1. 1 hit.
SMART	SM00288. VHS. 1 hit. [Graphical view]
PROSITE	PS50909. GAT. 1 hit. PS50179. VHS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301662.
PMAP-CutDB	O88746.
SOURCE	Search...

Entry information

Entry name

TOM1_MOUSE

Accession

Primary (citable) accession number: O88746
Secondary accession number(s): Q3V4C6, Q9D120

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 1, 1998
Last modified:	November 24, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)