O88736 (DHB7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 7
Short name=17-beta-HSD 7
Estradiol 17-beta-dehydrogenase 7
EC=1.1.1.62

Gene names

Name:

Hsd17b7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols.
Catalytic activity	A 3-beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH. 17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis. Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Subcellular location	Cell membrane; Single-pass membrane protein.
Tissue specificity	Most abundant in ovaries of pregnant animals. Present also in nonpregnant animals in ovaries, mammary gland, liver, kidney and testis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol biosynthetic process Inferred from direct assay PubMed 12829805. Source: MGI estrogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 12829805. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-keto sterol reductase activity Inferred from direct assay PubMed 12829805. Source: MGI estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	3-keto-steroid reductase		PRO_0000054587
Regions
	Topological domain	1 – 229	229	Extracellular Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Topological domain	251 – 334	84	Cytoplasmic Potential
	Nucleotide binding	8 – 15	8	NAD Potential
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	171	1	Substrate By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	178	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	160	1	A → V in CAC88119. Ref.2
	Sequence conflict	160	1	A → V in BC011464. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O88736 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E05F0716465BC160	FASTA	334	37,317
	10 20 30 40 50 60 MRKVVLITGA SSGIGLALCG RLLAEDDDLH LCLACRNLSK ARAVRDTLLA SHPSAEVSIV 70 80 90 100 110 120 QMDVSSLQSV VRGAEEVKQK FQRLDYLYLN AGILPNPQFN LKAFFCGIFS RNVIHMFTTA 130 140 150 160 170 180 EGILTQNDSV TADGLQEVFE TNLFGHFILI RELEPLLCHA DNPSQLIWTS SRNAKKANFS 190 200 210 220 230 240 LEDIQHSKGP EPYSSSKYAT DLLNVALNRN FNQKGLYSSV MCPGVVMTNM TYGILPPFIW 250 260 270 280 290 300 TLLLPIMWLL RFFVNALTVT PYNGAEALVW LFHQKPESLN PLTKYASATS GFGTNYVTGQ 310 320 330 KMDIDEDTAE KFYEVLLELE KRVRTTVQKS DHPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat." Nokelainen P., Peltoketo H., Vihko R., Vihko P. Mol. Endocrinol. 12:1048-1059(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Mammary gland.
[2]	"Characterization of mouse 17beta-hydroxysteroid dehydrogenase type 7 gene." Nokelainen P., Vihko P. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver and Placenta.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Characterization of mouse gene for 17-beta-hydroxysteroid dehydrogenase type 7." Ohnesorg T., Adamski J. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-301.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y15733 mRNA. Translation: CAA75742.1. AJ291459 , AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1. AK028380 mRNA. Translation: BAC25918.1. AK050211 mRNA. Translation: BAC34124.1. BC011464 mRNA. No translation available. AF367475 Genomic DNA. Translation: AAM21211.1.
IPI	IPI00316067.
RefSeq	NP_034606.3. NM_010476.3.
UniGene	Mm.12882.
3D structure databases
ProteinModelPortal	O88736.
SMR	O88736. Positions 3-235.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88736. 1 interaction.
PTM databases
PhosphoSite	O88736.
Proteomic databases
PaxDb	O88736.
PRIDE	O88736.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027989; ENSMUSP00000027989; ENSMUSG00000026675.
GeneID	15490.
KEGG	mmu:15490.
UCSC	uc007dlp.1. mouse.
Organism-specific databases
CTD	51478.
MGI	MGI:1330808. Hsd17b7.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	ENSGT00390000013340.
HOGENOM	HOG000253921.
HOVERGEN	HBG058236.
KO	K13373.
OMA	CHSDNPS.
OrthoDB	EOG4B8JDN.
Enzyme and pathway databases
UniPathway	UPA00769. UPA00770; UER00758.
Gene expression databases
ArrayExpress	O88736.
Bgee	O88736.
CleanEx	MM_HSD17B7.
Genevestigator	O88736.
GermOnline	ENSMUSG00000026675. Mus musculus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	288362.
SOURCE	Search...

Entry information

Entry name

DHB7_MOUSE

Accession

Primary (citable) accession number: O88736
Secondary accession number(s): Q8K5C9, Q921L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program