Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-keto-steroid reductase

Gene

Hsd17b7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the reduction of the keto group on the C-3 of sterols.

Catalytic activityi

A 3-beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH.
17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.

Pathwayi: estrogen biosynthesis

This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15NADSequence analysis8

GO - Molecular functioni

  • 3-keto sterol reductase activity Source: MGI
  • estradiol 17-beta-dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  • cholesterol biosynthetic process Source: MGI
  • estrogen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.1.1.270. 3474.
1.1.1.62. 3474.
ReactomeiR-MMU-191273. Cholesterol biosynthesis.
UniPathwayiUPA00769.
UPA00770; UER00758.

Chemistry databases

SwissLipidsiSLP:000001215.

Names & Taxonomyi

Protein namesi
Recommended name:
3-keto-steroid reductase (EC:1.1.1.270)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 7
Short name:
17-beta-HSD 7
Estradiol 17-beta-dehydrogenase 7 (EC:1.1.1.62)
Gene namesi
Name:Hsd17b7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1330808. Hsd17b7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 229ExtracellularSequence analysisAdd BLAST229
Transmembranei230 – 250HelicalSequence analysisAdd BLAST21
Topological domaini251 – 334CytoplasmicSequence analysisAdd BLAST84

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545871 – 3343-keto-steroid reductaseAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi229N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO88736.
MaxQBiO88736.
PaxDbiO88736.
PeptideAtlasiO88736.
PRIDEiO88736.

PTM databases

iPTMnetiO88736.
PhosphoSitePlusiO88736.

Expressioni

Tissue specificityi

Most abundant in ovaries of pregnant animals. Present also in nonpregnant animals in ovaries, mammary gland, liver, kidney and testis.

Gene expression databases

BgeeiENSMUSG00000026675.
CleanExiMM_HSD17B7.
ExpressionAtlasiO88736. baseline and differential.
GenevisibleiO88736. MM.

Interactioni

Protein-protein interaction databases

BioGridi200436. 1 interactor.
IntActiO88736. 1 interactor.
MINTiMINT-1862744.
STRINGi10090.ENSMUSP00000027989.

Structurei

3D structure databases

ProteinModelPortaliO88736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1478. Eukaryota.
ENOG410XPNE. LUCA.
GeneTreeiENSGT00390000013340.
HOGENOMiHOG000253921.
HOVERGENiHBG058236.
InParanoidiO88736.
KOiK13373.
OMAiWTLIMPI.
TreeFamiTF105433.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

O88736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKVVLITGA SSGIGLALCG RLLAEDDDLH LCLACRNLSK ARAVRDTLLA
60 70 80 90 100
SHPSAEVSIV QMDVSSLQSV VRGAEEVKQK FQRLDYLYLN AGILPNPQFN
110 120 130 140 150
LKAFFCGIFS RNVIHMFTTA EGILTQNDSV TADGLQEVFE TNLFGHFILI
160 170 180 190 200
RELEPLLCHA DNPSQLIWTS SRNAKKANFS LEDIQHSKGP EPYSSSKYAT
210 220 230 240 250
DLLNVALNRN FNQKGLYSSV MCPGVVMTNM TYGILPPFIW TLLLPIMWLL
260 270 280 290 300
RFFVNALTVT PYNGAEALVW LFHQKPESLN PLTKYASATS GFGTNYVTGQ
310 320 330
KMDIDEDTAE KFYEVLLELE KRVRTTVQKS DHPS
Length:334
Mass (Da):37,317
Last modified:November 1, 1998 - v1
Checksum:iE05F0716465BC160
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160A → V in CAC88119 (Ref. 2) Curated1
Sequence conflicti160A → V in BC011464 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15733 mRNA. Translation: CAA75742.1.
AJ291459
, AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1.
AK028380 mRNA. Translation: BAC25918.1.
AK050211 mRNA. Translation: BAC34124.1.
BC011464 mRNA. No translation available.
AF367475 Genomic DNA. Translation: AAM21211.1.
CCDSiCCDS35766.1.
RefSeqiNP_034606.3. NM_010476.3.
UniGeneiMm.12882.

Genome annotation databases

EnsembliENSMUST00000027989; ENSMUSP00000027989; ENSMUSG00000026675.
GeneIDi15490.
KEGGimmu:15490.
UCSCiuc007dlp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15733 mRNA. Translation: CAA75742.1.
AJ291459
, AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1.
AK028380 mRNA. Translation: BAC25918.1.
AK050211 mRNA. Translation: BAC34124.1.
BC011464 mRNA. No translation available.
AF367475 Genomic DNA. Translation: AAM21211.1.
CCDSiCCDS35766.1.
RefSeqiNP_034606.3. NM_010476.3.
UniGeneiMm.12882.

3D structure databases

ProteinModelPortaliO88736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200436. 1 interactor.
IntActiO88736. 1 interactor.
MINTiMINT-1862744.
STRINGi10090.ENSMUSP00000027989.

Chemistry databases

SwissLipidsiSLP:000001215.

PTM databases

iPTMnetiO88736.
PhosphoSitePlusiO88736.

Proteomic databases

EPDiO88736.
MaxQBiO88736.
PaxDbiO88736.
PeptideAtlasiO88736.
PRIDEiO88736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027989; ENSMUSP00000027989; ENSMUSG00000026675.
GeneIDi15490.
KEGGimmu:15490.
UCSCiuc007dlp.1. mouse.

Organism-specific databases

CTDi51478.
MGIiMGI:1330808. Hsd17b7.

Phylogenomic databases

eggNOGiKOG1478. Eukaryota.
ENOG410XPNE. LUCA.
GeneTreeiENSGT00390000013340.
HOGENOMiHOG000253921.
HOVERGENiHBG058236.
InParanoidiO88736.
KOiK13373.
OMAiWTLIMPI.
TreeFamiTF105433.

Enzyme and pathway databases

UniPathwayiUPA00769.
UPA00770; UER00758.
BRENDAi1.1.1.270. 3474.
1.1.1.62. 3474.
ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiO88736.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026675.
CleanExiMM_HSD17B7.
ExpressionAtlasiO88736. baseline and differential.
GenevisibleiO88736. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiDHB7_MOUSE
AccessioniPrimary (citable) accession number: O88736
Secondary accession number(s): Q8K5C9, Q921L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.