Reviewed,
UniProtKB/Swiss-Prot O88736 (DHB7_MOUSE)
Last modified
November 25, 2008.
Version 65.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 3-keto-steroid reductase EC=1.1.1.270 Alternative name(s): Estradiol 17-beta-dehydrogenase 7 EC=1.1.1.62 17-beta-hydroxysteroid dehydrogenase 7 Short name=17-beta-HSD 7 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 334 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Responsible for the reduction of the keto group on the C-3 of sterols. |
| Catalytic activity | 4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP(+) = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH. Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. |
| Pathway | Steroid biosynthesis; estrogen biosynthesis. Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6. |
| Subcellular location | |
| Tissue specificity | Most abundant in ovaries of pregnant animals. Present also in nonpregnant animals in ovaries, mammary gland, liver, kidney and testis. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily. |
Ontologies
Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis |
| Cellular component | Membrane |
| Domain | Transmembrane |
| Ligand | NAD NADP |
| Molecular function | Oxidoreductase |
| PTM | Glycoprotein |
Gene Ontology (GO) | |
| Biological process | cholesterol biosynthetic process Inferred from direct assay. Source: MGI oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from direct assay. Source: MGI integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-keto sterol reductase activity Inferred from direct assay. Source: MGI 3-keto-steroid reductase activityInferred from electronic annotation. Source: EC bindingInferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 334 | 334 | 3-keto-steroid reductase | PRO_0000054587 | |||||
Regions | |||||||||
| Topological domain | 1 – 229 | 229 | Extracellular Potential | ||||||
| Transmembrane | 230 – 250 | 21 | Potential | ||||||
| Topological domain | 251 – 334 | 84 | Cytoplasmic Potential | ||||||
| Nucleotide binding | 8 – 15 | 8 | NAD Potential | ||||||
Sites | |||||||||
| Active site | 193 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 171 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 37 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 127 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 178 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 229 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 160 | 1 | A → V Ref.2 Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat." Nokelainen P., Peltoketo H., Vihko R., Vihko P. Mol. Endocrinol. 12:1048-1059(1998) [PubMed: 9658408] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Mammary gland. |
| [2] | "Characterization of mouse 17beta-hydroxysteroid dehydrogenase type 7 gene." Nokelainen P., Vihko P. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.  Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver and Placenta. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor. |
| [5] | "Characterization of mouse gene for 17-beta-hydroxysteroid dehydrogenase type 7." Ohnesorg T., Adamski J. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-301. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Y15733 mRNA. Translation: CAA75742.1. AJ291459 AJ291462 Genomic DNA. Translation: CAC88119.1. AK028380 mRNA. Translation: BAC25918.1. AK050211 mRNA. Translation: BAC34124.1. BC011464 mRNA. Translation: AAH11464.1. AF367475 Genomic DNA. Translation: AAM21211.1. | |
| RefSeq | NP_034606.3. |
| UniGene | Mm.12882 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | O88736. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000026675. Mus musculus. [Contig view] |
| GeneID | 15490. |
| KEGG | mmu:15490. |
Organism-specific databases | |
| MGI | MGI:1330808. Hsd17b7. |
Phylogenomic databases | |
| HOGENOM | O88736. |
| HOVERGEN | O88736. |
Gene expression databases | |
| ArrayExpress | O88736. |
| CleanEx | MM_HSD17B7. |
| GermOnline | ENSMUSG00000026675. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR19410. ADH_short_C2. 1 hit. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. |
| PROSITE | PS00061. ADH_SHORT. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 288362. |
| SOURCE | Search... |
Entry information
| Entry name | DHB7_MOUSE | ||||||||
| Accession | Primary (citable) accession number: O88736 Secondary accession number(s): Q8K5C9, Q921L1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
