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Reviewed, UniProtKB/Swiss-Prot O88736 (DHB7_MOUSE)

Last modified November 25, 2008. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    3-keto-steroid reductase
    EC=1.1.1.270
Alternative name(s):
    Estradiol 17-beta-dehydrogenase 7
    EC=1.1.1.62
    17-beta-hydroxysteroid dehydrogenase 7
      Short name=17-beta-HSD 7
Gene names
Name: Hsd17b7
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Responsible for the reduction of the keto group on the C-3 of sterols.

Catalytic activity

4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP(+) = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.

Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H.

Pathway

Steroid biosynthesis; estrogen biosynthesis.

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.

Subcellular location

Membrane; Single-pass membrane protein.

Tissue specificity

Most abundant in ovaries of pregnant animals. Present also in nonpregnant animals in ovaries, mammary gland, liver, kidney and testis.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3343343-keto-steroid reductase
PRO_0000054587

Regions

Topological domain1 – 229229Extracellular Potential
Transmembrane230 – 25021 Potential
Topological domain251 – 33484Cytoplasmic Potential
Nucleotide binding8 – 158NAD Potential

Sites

Active site1931Proton acceptor By similarity
Binding site1711Substrate By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1601A → V Ref.2 Ref.4

Sequences

Sequence LengthMass (Da)Tools
O88736-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E05F0716465BC160

FASTA33437,317
        10         20         30         40         50         60 
MRKVVLITGA SSGIGLALCG RLLAEDDDLH LCLACRNLSK ARAVRDTLLA SHPSAEVSIV 

        70         80         90        100        110        120 
QMDVSSLQSV VRGAEEVKQK FQRLDYLYLN AGILPNPQFN LKAFFCGIFS RNVIHMFTTA 

       130        140        150        160        170        180 
EGILTQNDSV TADGLQEVFE TNLFGHFILI RELEPLLCHA DNPSQLIWTS SRNAKKANFS 

       190        200        210        220        230        240 
LEDIQHSKGP EPYSSSKYAT DLLNVALNRN FNQKGLYSSV MCPGVVMTNM TYGILPPFIW 

       250        260        270        280        290        300 
TLLLPIMWLL RFFVNALTVT PYNGAEALVW LFHQKPESLN PLTKYASATS GFGTNYVTGQ 

       310        320        330 
KMDIDEDTAE KFYEVLLELE KRVRTTVQKS DHPS 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat."
Nokelainen P., Peltoketo H., Vihko R., Vihko P.
Mol. Endocrinol. 12:1048-1059(1998) [PubMed: 9658408] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Mammary gland.
[2]"Characterization of mouse 17beta-hydroxysteroid dehydrogenase type 7 gene."
Nokelainen P., Vihko P.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Liver and Placenta.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Characterization of mouse gene for 17-beta-hydroxysteroid dehydrogenase type 7."
Ohnesorg T., Adamski J.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-301.
+Additional computationally mapped references.

Cross-references

Sequence databases

Y15733 mRNA. Translation: CAA75742.1.
AJ291459 expand/collapse EMBL AC list , AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1.
AK028380 mRNA. Translation: BAC25918.1.
AK050211 mRNA. Translation: BAC34124.1.
BC011464 mRNA. Translation: AAH11464.1.
AF367475 Genomic DNA. Translation: AAM21211.1.
RefSeqNP_034606.3.
UniGeneMm.12882

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteO88736.

Genome annotation databases

EnsemblENSMUSG00000026675. Mus musculus. [Contig view]
GeneID15490.
KEGGmmu:15490.

Organism-specific databases

MGIMGI:1330808. Hsd17b7.

Phylogenomic databases

HOGENOMO88736.
HOVERGENO88736.

Gene expression databases

ArrayExpressO88736.
CleanExMM_HSD17B7.
GermOnlineENSMUSG00000026675. Mus musculus.

Family and domain databases

InterProIPR002198. DHase_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR19410. ADH_short_C2. 1 hit.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio288362.
SOURCESearch...

Entry information

Entry nameDHB7_MOUSE
AccessionPrimary (citable) accession number: O88736
Secondary accession number(s): Q8K5C9, Q921L1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents