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Protein

3-keto-steroid reductase

Gene

Hsd17b7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the reduction of the keto group on the C-3 of sterols.

Catalytic activityi

A 3-beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH.
17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711SubstrateBy similarity
Active sitei193 – 1931Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158NADSequence Analysis

GO - Molecular functioni

  1. 3-keto sterol reductase activity Source: MGI
  2. estradiol 17-beta-dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol biosynthetic process Source: MGI
  2. estrogen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.1.1.270. 3474.
1.1.1.62. 3474.
ReactomeiREACT_345006. Cholesterol biosynthesis.
UniPathwayiUPA00769.
UPA00770; UER00758.

Names & Taxonomyi

Protein namesi
Recommended name:
3-keto-steroid reductase (EC:1.1.1.270)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 7
Short name:
17-beta-HSD 7
Estradiol 17-beta-dehydrogenase 7 (EC:1.1.1.62)
Gene namesi
Name:Hsd17b7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1330808. Hsd17b7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 229229ExtracellularSequence AnalysisAdd
BLAST
Transmembranei230 – 25021HelicalSequence AnalysisAdd
BLAST
Topological domaini251 – 33484CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3343343-keto-steroid reductasePRO_0000054587Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO88736.
PaxDbiO88736.
PRIDEiO88736.

PTM databases

PhosphoSiteiO88736.

Expressioni

Tissue specificityi

Most abundant in ovaries of pregnant animals. Present also in nonpregnant animals in ovaries, mammary gland, liver, kidney and testis.

Gene expression databases

BgeeiO88736.
CleanExiMM_HSD17B7.
ExpressionAtlasiO88736. baseline and differential.
GenevestigatoriO88736.

Interactioni

Protein-protein interaction databases

BioGridi200436. 1 interaction.
IntActiO88736. 1 interaction.
MINTiMINT-1862744.

Structurei

3D structure databases

ProteinModelPortaliO88736.
SMRiO88736. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00390000013340.
HOGENOMiHOG000253921.
HOVERGENiHBG058236.
InParanoidiO88736.
KOiK13373.
OMAiCHSDNPS.
OrthoDBiEOG7S4X6D.
TreeFamiTF105433.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

O88736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKVVLITGA SSGIGLALCG RLLAEDDDLH LCLACRNLSK ARAVRDTLLA
60 70 80 90 100
SHPSAEVSIV QMDVSSLQSV VRGAEEVKQK FQRLDYLYLN AGILPNPQFN
110 120 130 140 150
LKAFFCGIFS RNVIHMFTTA EGILTQNDSV TADGLQEVFE TNLFGHFILI
160 170 180 190 200
RELEPLLCHA DNPSQLIWTS SRNAKKANFS LEDIQHSKGP EPYSSSKYAT
210 220 230 240 250
DLLNVALNRN FNQKGLYSSV MCPGVVMTNM TYGILPPFIW TLLLPIMWLL
260 270 280 290 300
RFFVNALTVT PYNGAEALVW LFHQKPESLN PLTKYASATS GFGTNYVTGQ
310 320 330
KMDIDEDTAE KFYEVLLELE KRVRTTVQKS DHPS
Length:334
Mass (Da):37,317
Last modified:October 31, 1998 - v1
Checksum:iE05F0716465BC160
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601A → V in CAC88119 (Ref. 2) Curated
Sequence conflicti160 – 1601A → V in BC011464 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15733 mRNA. Translation: CAA75742.1.
AJ291459
, AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1.
AK028380 mRNA. Translation: BAC25918.1.
AK050211 mRNA. Translation: BAC34124.1.
BC011464 mRNA. No translation available.
AF367475 Genomic DNA. Translation: AAM21211.1.
CCDSiCCDS35766.1.
RefSeqiNP_034606.3. NM_010476.3.
UniGeneiMm.12882.

Genome annotation databases

EnsembliENSMUST00000027989; ENSMUSP00000027989; ENSMUSG00000026675.
GeneIDi15490.
KEGGimmu:15490.
UCSCiuc007dlp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15733 mRNA. Translation: CAA75742.1.
AJ291459
, AJ291460, AJ291461, AJ291463, AJ291465, AJ291466, AJ291464, AJ291462 Genomic DNA. Translation: CAC88119.1.
AK028380 mRNA. Translation: BAC25918.1.
AK050211 mRNA. Translation: BAC34124.1.
BC011464 mRNA. No translation available.
AF367475 Genomic DNA. Translation: AAM21211.1.
CCDSiCCDS35766.1.
RefSeqiNP_034606.3. NM_010476.3.
UniGeneiMm.12882.

3D structure databases

ProteinModelPortaliO88736.
SMRiO88736. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200436. 1 interaction.
IntActiO88736. 1 interaction.
MINTiMINT-1862744.

PTM databases

PhosphoSiteiO88736.

Proteomic databases

MaxQBiO88736.
PaxDbiO88736.
PRIDEiO88736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027989; ENSMUSP00000027989; ENSMUSG00000026675.
GeneIDi15490.
KEGGimmu:15490.
UCSCiuc007dlp.1. mouse.

Organism-specific databases

CTDi51478.
MGIiMGI:1330808. Hsd17b7.

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00390000013340.
HOGENOMiHOG000253921.
HOVERGENiHBG058236.
InParanoidiO88736.
KOiK13373.
OMAiCHSDNPS.
OrthoDBiEOG7S4X6D.
TreeFamiTF105433.

Enzyme and pathway databases

UniPathwayiUPA00769.
UPA00770; UER00758.
BRENDAi1.1.1.270. 3474.
1.1.1.62. 3474.
ReactomeiREACT_345006. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi288362.
PROiO88736.
SOURCEiSearch...

Gene expression databases

BgeeiO88736.
CleanExiMM_HSD17B7.
ExpressionAtlasiO88736. baseline and differential.
GenevestigatoriO88736.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat."
    Nokelainen P., Peltoketo H., Vihko R., Vihko P.
    Mol. Endocrinol. 12:1048-1059(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Mammary gland.
  2. "Characterization of mouse 17beta-hydroxysteroid dehydrogenase type 7 gene."
    Nokelainen P., Vihko P.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver and Placenta.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Characterization of mouse gene for 17-beta-hydroxysteroid dehydrogenase type 7."
    Ohnesorg T., Adamski J.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 1-301.

Entry informationi

Entry nameiDHB7_MOUSE
AccessioniPrimary (citable) accession number: O88736
Secondary accession number(s): Q8K5C9, Q921L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: October 31, 1998
Last modified: March 31, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.