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O88735

- MAP7_MOUSE

UniProt

O88735 - MAP7_MOUSE

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Protein
Ensconsin
Gene
Map7, Mtap7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.1 Publication

GO - Molecular functioni

  1. protein binding Source: MGI
  2. receptor binding Source: BHF-UCL

GO - Biological processi

  1. Leydig cell differentiation Source: MGI
  2. Sertoli cell development Source: MGI
  3. cell morphogenesis Source: MGI
  4. cell proliferation Source: MGI
  5. fertilization Source: MGI
  6. germ cell development Source: MGI
  7. glycosphingolipid metabolic process Source: MGI
  8. homeostasis of number of cells Source: MGI
  9. microtubule bundle formation Source: MGI
  10. nucleus organization Source: MGI
  11. organ growth Source: MGI
  12. protein localization to plasma membrane Source: BHF-UCL
  13. response to osmotic stress Source: BHF-UCL
  14. response to retinoic acid Source: MGI
  15. spermatogenesis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ensconsin
Alternative name(s):
Epithelial microtubule-associated protein of 115 kDa
Short name:
E-MAP-115
Microtubule-associated protein 7
Short name:
MAP-7
Gene namesi
Name:Map7
Synonyms:Mtap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1328328. Map7.

Subcellular locationi

Cytoplasmperinuclear region. Basolateral cell membrane. Cytoplasmcytoskeleton
Note: Colocalized on microtubules. An intracellular redistribution is triggered during induction of keratinocyte terminal differentiation from microtubules with a perinuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts.1 Publication

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. microtubule Source: UniProtKB-KW
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 730729Ensconsin
PRO_0000255950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei183 – 1831Phosphoserine By similarity
Modified residuei209 – 2091Phosphoserine By similarity
Modified residuei219 – 2191Phosphoserine By similarity
Modified residuei231 – 2311Phosphothreonine By similarity
Modified residuei254 – 2541Phosphoserine By similarity
Modified residuei277 – 2771Phosphothreonine By similarity

Post-translational modificationi

The association with microtubules is regulated by phosphorylation during the cell cycle. During interphase only phosphorylated on serine. Phosphorylated on threonine in mitosis By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO88735.
PaxDbiO88735.
PRIDEiO88735.

PTM databases

PhosphoSiteiO88735.

Expressioni

Tissue specificityi

Highly expressed in the epithelial cells of the kidney tubules and in the absorptive cells of the intestine, and widely distributed in the testis. Expression correlates with the differentiation of certain epithelial cell types: in the adult intestine, more abundantly expressed in the differentiating than in the proliferative cell compartment. The expression clearly correlates with the degree of cellular apicobasal polarity. Expressed in lung, kidney, brain and fat. Colocalized with TRPV4 in ependymal cells, in the choroid plexus, in bronchial and renal cortical tubular cells. Widely expressed in excitable neuronal cells, vascular cells as well as in epithelial cells. In seminiferous epithelium, associated with the microtubule of the spermatid manchette.3 Publications

Developmental stagei

Expressed in several epithelia from E9.5 onwards, with expression levels increasing during development. From E14.5 onwards, found in some neuronal cells as well.1 Publication

Gene expression databases

CleanExiMM_MTAP7.
GenevestigatoriO88735.

Interactioni

Subunit structurei

Interacts with TRPV4.1 Publication

Protein-protein interaction databases

IntActiO88735. 1 interaction.
MINTiMINT-4105617.

Structurei

3D structure databases

ProteinModelPortaliO88735.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili89 – 15264 Reviewed prediction
Add
BLAST
Coiled coili460 – 589130 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the MAP7 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG46082.
HOGENOMiHOG000231569.
HOVERGENiHBG081952.
KOiK10433.
PhylomeDBiO88735.

Family and domain databases

InterProiIPR008604. MAP7.
[Graphical view]
PANTHERiPTHR15073. PTHR15073. 1 hit.
PfamiPF05672. MAP7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88735-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEQGAGGDG HRGGDGATHS DPASDGYKVQ EKRTAPSRPT STVSGQTSNH    50
SGNKPDPPPV LRVDDRQRLA RERREEREKQ LAARETVWLE REERARQHYE 100
RHLEARKKKL EDQRLKEERR RAAVEEKRRQ RLEEDKERHE AVVRRTMERS 150
QKPRQKSNRW SWGSPLHGSS SIHSGDPDRR SVSTMNLSKH VDPVISKRLS 200
SSSATLLNSP DRARRLQLSP WESSVVSRLL TPTHSFLARS KSTAALSGDT 250
ASCSPIIMPF KAAHSRNPVD RPKLFVTPPE GSARRRTIHG LASHKRERER 300
EHVPFHVSPG ARRTLSPSNL KARSPAPARL WLPSKSMPHL PGTPRPASSL 350
PPGSVRAASA QAPSSSPGNI RPFKREVKVE PEKKDPLPAV KSRVPLVKVE 400
EVTVEEGTPV KPPEPAAPAS APIATPAPAP ATDPAPVPAP SSTVTVGVVP 450
KTSAGTTDPE EATRLLAEKR RLAREQREKE ERERKEKEEL ERQKIEELAR 500
RVAEERSRRE EEARRLEEEQ AREKEELALR LAEEERERWE REEVERVQKQ 550
KEEEARAREE AERARQEREK HFQKEEQERL ERKKRLEEIM RRTRRTETAD 600
KKTTEQRNGD IAKGVLTGEP EVPALPCMAS SGNGESAESP HGVALQQSEV 650
TTESSPDLEK QPNENGMSIQ NENFEEVINL PVGSKASRLD VTNENPEIPL 700
KPILAFNDEG TLGPLPQVDG VQTQQTAEVI 730
Length:730
Mass (Da):82,022
Last modified:November 1, 1998 - v1
Checksum:iF44B4201DE8F5A17
GO
Isoform 2 (identifier: O88735-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-250: T → TVIPICPRS

Note: No experimental confirmation available.

Show »
Length:738
Mass (Da):82,888
Checksum:i8CEAE46EE589C5EE
GO

Sequence cautioni

The sequence AAH42771.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei250 – 2501T → TVIPICPRS in isoform 2.
VSP_021318

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41Q → H in BAE21585. 1 Publication
Sequence conflicti8 – 81G → C in BAE21585. 1 Publication
Sequence conflicti16 – 161G → S in BAE21585. 1 Publication
Sequence conflicti35 – 351A → G in BAE21585. 1 Publication
Sequence conflicti360 – 3601A → T in AAH42771. 1 Publication
Sequence conflicti373 – 3731F → V in BAE21585. 1 Publication
Sequence conflicti373 – 3731F → V in AAH52637. 1 Publication
Sequence conflicti373 – 3731F → V in AAH42771. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y15197 mRNA. Translation: CAA75495.1.
AB098611 mRNA. Translation: BAC53729.1.
AK133267 mRNA. Translation: BAE21585.1.
BC042771 mRNA. Translation: AAH42771.1. Sequence problems.
BC052637 mRNA. Translation: AAH52637.1.
CCDSiCCDS56694.1. [O88735-2]
RefSeqiNP_001185564.1. NM_001198635.1.
NP_032661.2. NM_008635.2.
UniGeneiMm.20928.

Genome annotation databases

GeneIDi17761.
KEGGimmu:17761.
UCSCiuc007eno.2. mouse. [O88735-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y15197 mRNA. Translation: CAA75495.1 .
AB098611 mRNA. Translation: BAC53729.1 .
AK133267 mRNA. Translation: BAE21585.1 .
BC042771 mRNA. Translation: AAH42771.1 . Sequence problems.
BC052637 mRNA. Translation: AAH52637.1 .
CCDSi CCDS56694.1. [O88735-2 ]
RefSeqi NP_001185564.1. NM_001198635.1.
NP_032661.2. NM_008635.2.
UniGenei Mm.20928.

3D structure databases

ProteinModelPortali O88735.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88735. 1 interaction.
MINTi MINT-4105617.

PTM databases

PhosphoSitei O88735.

Proteomic databases

MaxQBi O88735.
PaxDbi O88735.
PRIDEi O88735.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17761.
KEGGi mmu:17761.
UCSCi uc007eno.2. mouse. [O88735-1 ]

Organism-specific databases

CTDi 9053.
MGIi MGI:1328328. Map7.

Phylogenomic databases

eggNOGi NOG46082.
HOGENOMi HOG000231569.
HOVERGENi HBG081952.
KOi K10433.
PhylomeDBi O88735.

Miscellaneous databases

NextBioi 292449.
PROi O88735.
SOURCEi Search...

Gene expression databases

CleanExi MM_MTAP7.
Genevestigatori O88735.

Family and domain databases

InterProi IPR008604. MAP7.
[Graphical view ]
PANTHERi PTHR15073. PTHR15073. 1 hit.
Pfami PF05672. MAP7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The distribution of murine 115-kDa epithelial microtubule-associated protein (E-MAP-115) during embryogenesis and in adult organs suggests a role in epithelial polarization and differentiation."
    Fabre-Jonca N., Allaman J.-M., Radlgruber G., Meda P., Kiss J.Z., French L.E., Masson D.
    Differentiation 63:169-180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6 X CBA.
  2. "Microtubule-associated protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4)."
    Suzuki M., Hirao A., Mizuno A.
    J. Biol. Chem. 278:51448-51453(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TRPV4.
    Tissue: Kidney.
  3. Erratum
    Suzuki M., Hirao A., Mizuno A.
    J. Biol. Chem. 280:25948-25948(2005)
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-494 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Eye and Mammary gland.
  6. "Microtubule-associated epithelial protein E-MAP-115 is localized in the spermatid manchette."
    Penttilae T.-L., Parvinen M., Paranko J.
    Int. J. Androl. 26:166-174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAP7_MOUSE
AccessioniPrimary (citable) accession number: O88735
Secondary accession number(s): Q3V0B9, Q7TQL9, Q80V60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi