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O88735

- MAP7_MOUSE

UniProt

O88735 - MAP7_MOUSE

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Protein

Ensconsin

Gene

Map7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.1 Publication

GO - Molecular functioni

  1. receptor binding Source: BHF-UCL

GO - Biological processi

  1. cell morphogenesis Source: MGI
  2. cell proliferation Source: MGI
  3. fertilization Source: MGI
  4. germ cell development Source: MGI
  5. glycosphingolipid metabolic process Source: MGI
  6. homeostasis of number of cells Source: MGI
  7. Leydig cell differentiation Source: MGI
  8. microtubule bundle formation Source: MGI
  9. nucleus organization Source: MGI
  10. organ growth Source: MGI
  11. protein localization to plasma membrane Source: BHF-UCL
  12. response to osmotic stress Source: BHF-UCL
  13. response to retinoic acid Source: MGI
  14. Sertoli cell development Source: MGI
  15. spermatogenesis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ensconsin
Alternative name(s):
Epithelial microtubule-associated protein of 115 kDa
Short name:
E-MAP-115
Microtubule-associated protein 7
Short name:
MAP-7
Gene namesi
Name:Map7
Synonyms:Mtap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1328328. Map7.

Subcellular locationi

Cytoplasmperinuclear region 1 Publication. Basolateral cell membrane 1 Publication. Cytoplasmcytoskeleton 1 Publication
Note: Colocalized on microtubules. An intracellular redistribution is triggered during induction of keratinocyte terminal differentiation from microtubules with a perinuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. microtubule Source: UniProtKB-KW
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 730729EnsconsinPRO_0000255950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei209 – 2091PhosphoserineBy similarity
Modified residuei219 – 2191PhosphoserineBy similarity
Modified residuei231 – 2311PhosphothreonineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity
Modified residuei277 – 2771PhosphothreonineBy similarity

Post-translational modificationi

The association with microtubules is regulated by phosphorylation during the cell cycle. During interphase only phosphorylated on serine. Phosphorylated on threonine in mitosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO88735.
PaxDbiO88735.
PRIDEiO88735.

PTM databases

PhosphoSiteiO88735.

Expressioni

Tissue specificityi

Highly expressed in the epithelial cells of the kidney tubules and in the absorptive cells of the intestine, and widely distributed in the testis. Expression correlates with the differentiation of certain epithelial cell types: in the adult intestine, more abundantly expressed in the differentiating than in the proliferative cell compartment. The expression clearly correlates with the degree of cellular apicobasal polarity. Expressed in lung, kidney, brain and fat. Colocalized with TRPV4 in ependymal cells, in the choroid plexus, in bronchial and renal cortical tubular cells. Widely expressed in excitable neuronal cells, vascular cells as well as in epithelial cells. In seminiferous epithelium, associated with the microtubule of the spermatid manchette.3 Publications

Developmental stagei

Expressed in several epithelia from E9.5 onwards, with expression levels increasing during development. From E14.5 onwards, found in some neuronal cells as well.1 Publication

Gene expression databases

CleanExiMM_MTAP7.
GenevestigatoriO88735.

Interactioni

Subunit structurei

Interacts with TRPV4.1 Publication

Protein-protein interaction databases

IntActiO88735. 1 interaction.
MINTiMINT-4105617.

Structurei

3D structure databases

ProteinModelPortaliO88735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili89 – 15264Sequence AnalysisAdd
BLAST
Coiled coili460 – 589130Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the MAP7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG46082.
HOGENOMiHOG000231569.
HOVERGENiHBG081952.
InParanoidiO88735.
KOiK10433.
PhylomeDBiO88735.

Family and domain databases

InterProiIPR008604. MAP7.
[Graphical view]
PANTHERiPTHR15073. PTHR15073. 1 hit.
PfamiPF05672. MAP7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88735-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEQGAGGDG HRGGDGATHS DPASDGYKVQ EKRTAPSRPT STVSGQTSNH
60 70 80 90 100
SGNKPDPPPV LRVDDRQRLA RERREEREKQ LAARETVWLE REERARQHYE
110 120 130 140 150
RHLEARKKKL EDQRLKEERR RAAVEEKRRQ RLEEDKERHE AVVRRTMERS
160 170 180 190 200
QKPRQKSNRW SWGSPLHGSS SIHSGDPDRR SVSTMNLSKH VDPVISKRLS
210 220 230 240 250
SSSATLLNSP DRARRLQLSP WESSVVSRLL TPTHSFLARS KSTAALSGDT
260 270 280 290 300
ASCSPIIMPF KAAHSRNPVD RPKLFVTPPE GSARRRTIHG LASHKRERER
310 320 330 340 350
EHVPFHVSPG ARRTLSPSNL KARSPAPARL WLPSKSMPHL PGTPRPASSL
360 370 380 390 400
PPGSVRAASA QAPSSSPGNI RPFKREVKVE PEKKDPLPAV KSRVPLVKVE
410 420 430 440 450
EVTVEEGTPV KPPEPAAPAS APIATPAPAP ATDPAPVPAP SSTVTVGVVP
460 470 480 490 500
KTSAGTTDPE EATRLLAEKR RLAREQREKE ERERKEKEEL ERQKIEELAR
510 520 530 540 550
RVAEERSRRE EEARRLEEEQ AREKEELALR LAEEERERWE REEVERVQKQ
560 570 580 590 600
KEEEARAREE AERARQEREK HFQKEEQERL ERKKRLEEIM RRTRRTETAD
610 620 630 640 650
KKTTEQRNGD IAKGVLTGEP EVPALPCMAS SGNGESAESP HGVALQQSEV
660 670 680 690 700
TTESSPDLEK QPNENGMSIQ NENFEEVINL PVGSKASRLD VTNENPEIPL
710 720 730
KPILAFNDEG TLGPLPQVDG VQTQQTAEVI
Length:730
Mass (Da):82,022
Last modified:November 1, 1998 - v1
Checksum:iF44B4201DE8F5A17
GO
Isoform 2 (identifier: O88735-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-250: T → TVIPICPRS

Note: No experimental confirmation available.

Show »
Length:738
Mass (Da):82,888
Checksum:i8CEAE46EE589C5EE
GO

Sequence cautioni

The sequence AAH42771.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41Q → H in BAE21585. (PubMed:16141072)Curated
Sequence conflicti8 – 81G → C in BAE21585. (PubMed:16141072)Curated
Sequence conflicti16 – 161G → S in BAE21585. (PubMed:16141072)Curated
Sequence conflicti35 – 351A → G in BAE21585. (PubMed:16141072)Curated
Sequence conflicti360 – 3601A → T in AAH42771. (PubMed:15489334)Curated
Sequence conflicti373 – 3731F → V in BAE21585. (PubMed:16141072)Curated
Sequence conflicti373 – 3731F → V in AAH52637. (PubMed:15489334)Curated
Sequence conflicti373 – 3731F → V in AAH42771. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei250 – 2501T → TVIPICPRS in isoform 2. 1 PublicationVSP_021318

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15197 mRNA. Translation: CAA75495.1.
AB098611 mRNA. Translation: BAC53729.1.
AK133267 mRNA. Translation: BAE21585.1.
BC042771 mRNA. Translation: AAH42771.1. Sequence problems.
BC052637 mRNA. Translation: AAH52637.1.
CCDSiCCDS56694.1. [O88735-2]
RefSeqiNP_001185564.1. NM_001198635.1.
NP_032661.2. NM_008635.2.
UniGeneiMm.20928.

Genome annotation databases

GeneIDi17761.
KEGGimmu:17761.
UCSCiuc007eno.2. mouse. [O88735-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15197 mRNA. Translation: CAA75495.1 .
AB098611 mRNA. Translation: BAC53729.1 .
AK133267 mRNA. Translation: BAE21585.1 .
BC042771 mRNA. Translation: AAH42771.1 . Sequence problems.
BC052637 mRNA. Translation: AAH52637.1 .
CCDSi CCDS56694.1. [O88735-2 ]
RefSeqi NP_001185564.1. NM_001198635.1.
NP_032661.2. NM_008635.2.
UniGenei Mm.20928.

3D structure databases

ProteinModelPortali O88735.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O88735. 1 interaction.
MINTi MINT-4105617.

PTM databases

PhosphoSitei O88735.

Proteomic databases

MaxQBi O88735.
PaxDbi O88735.
PRIDEi O88735.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17761.
KEGGi mmu:17761.
UCSCi uc007eno.2. mouse. [O88735-1 ]

Organism-specific databases

CTDi 9053.
MGIi MGI:1328328. Map7.

Phylogenomic databases

eggNOGi NOG46082.
HOGENOMi HOG000231569.
HOVERGENi HBG081952.
InParanoidi O88735.
KOi K10433.
PhylomeDBi O88735.

Miscellaneous databases

NextBioi 292449.
PROi O88735.
SOURCEi Search...

Gene expression databases

CleanExi MM_MTAP7.
Genevestigatori O88735.

Family and domain databases

InterProi IPR008604. MAP7.
[Graphical view ]
PANTHERi PTHR15073. PTHR15073. 1 hit.
Pfami PF05672. MAP7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The distribution of murine 115-kDa epithelial microtubule-associated protein (E-MAP-115) during embryogenesis and in adult organs suggests a role in epithelial polarization and differentiation."
    Fabre-Jonca N., Allaman J.-M., Radlgruber G., Meda P., Kiss J.Z., French L.E., Masson D.
    Differentiation 63:169-180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6 X CBA.
  2. "Microtubule-associated protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4)."
    Suzuki M., Hirao A., Mizuno A.
    J. Biol. Chem. 278:51448-51453(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TRPV4.
    Tissue: Kidney.
  3. Erratum
    Suzuki M., Hirao A., Mizuno A.
    J. Biol. Chem. 280:25948-25948(2005)
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-494 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Eye and Mammary gland.
  6. "Microtubule-associated epithelial protein E-MAP-115 is localized in the spermatid manchette."
    Penttilae T.-L., Parvinen M., Paranko J.
    Int. J. Androl. 26:166-174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAP7_MOUSE
AccessioniPrimary (citable) accession number: O88735
Secondary accession number(s): Q3V0B9, Q7TQL9, Q80V60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3