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O88735

- MAP7_MOUSE

UniProt

O88735 - MAP7_MOUSE

Protein

Ensconsin

Gene

Map7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. receptor binding Source: BHF-UCL

    GO - Biological processi

    1. cell morphogenesis Source: MGI
    2. cell proliferation Source: MGI
    3. fertilization Source: MGI
    4. germ cell development Source: MGI
    5. glycosphingolipid metabolic process Source: MGI
    6. homeostasis of number of cells Source: MGI
    7. Leydig cell differentiation Source: MGI
    8. microtubule bundle formation Source: MGI
    9. nucleus organization Source: MGI
    10. organ growth Source: MGI
    11. protein localization to plasma membrane Source: BHF-UCL
    12. response to osmotic stress Source: BHF-UCL
    13. response to retinoic acid Source: MGI
    14. Sertoli cell development Source: MGI
    15. spermatogenesis Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ensconsin
    Alternative name(s):
    Epithelial microtubule-associated protein of 115 kDa
    Short name:
    E-MAP-115
    Microtubule-associated protein 7
    Short name:
    MAP-7
    Gene namesi
    Name:Map7
    Synonyms:Mtap7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1328328. Map7.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication. Basolateral cell membrane 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Colocalized on microtubules. An intracellular redistribution is triggered during induction of keratinocyte terminal differentiation from microtubules with a perinuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. microtubule Source: UniProtKB-KW
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 730729EnsconsinPRO_0000255950Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei183 – 1831PhosphoserineBy similarity
    Modified residuei209 – 2091PhosphoserineBy similarity
    Modified residuei219 – 2191PhosphoserineBy similarity
    Modified residuei231 – 2311PhosphothreonineBy similarity
    Modified residuei254 – 2541PhosphoserineBy similarity
    Modified residuei277 – 2771PhosphothreonineBy similarity

    Post-translational modificationi

    The association with microtubules is regulated by phosphorylation during the cell cycle. During interphase only phosphorylated on serine. Phosphorylated on threonine in mitosis By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO88735.
    PaxDbiO88735.
    PRIDEiO88735.

    PTM databases

    PhosphoSiteiO88735.

    Expressioni

    Tissue specificityi

    Highly expressed in the epithelial cells of the kidney tubules and in the absorptive cells of the intestine, and widely distributed in the testis. Expression correlates with the differentiation of certain epithelial cell types: in the adult intestine, more abundantly expressed in the differentiating than in the proliferative cell compartment. The expression clearly correlates with the degree of cellular apicobasal polarity. Expressed in lung, kidney, brain and fat. Colocalized with TRPV4 in ependymal cells, in the choroid plexus, in bronchial and renal cortical tubular cells. Widely expressed in excitable neuronal cells, vascular cells as well as in epithelial cells. In seminiferous epithelium, associated with the microtubule of the spermatid manchette.3 Publications

    Developmental stagei

    Expressed in several epithelia from E9.5 onwards, with expression levels increasing during development. From E14.5 onwards, found in some neuronal cells as well.1 Publication

    Gene expression databases

    CleanExiMM_MTAP7.
    GenevestigatoriO88735.

    Interactioni

    Subunit structurei

    Interacts with TRPV4.1 Publication

    Protein-protein interaction databases

    IntActiO88735. 1 interaction.
    MINTiMINT-4105617.

    Structurei

    3D structure databases

    ProteinModelPortaliO88735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili89 – 15264Sequence AnalysisAdd
    BLAST
    Coiled coili460 – 589130Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MAP7 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG46082.
    HOGENOMiHOG000231569.
    HOVERGENiHBG081952.
    KOiK10433.
    PhylomeDBiO88735.

    Family and domain databases

    InterProiIPR008604. MAP7.
    [Graphical view]
    PANTHERiPTHR15073. PTHR15073. 1 hit.
    PfamiPF05672. MAP7. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O88735-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEQGAGGDG HRGGDGATHS DPASDGYKVQ EKRTAPSRPT STVSGQTSNH    50
    SGNKPDPPPV LRVDDRQRLA RERREEREKQ LAARETVWLE REERARQHYE 100
    RHLEARKKKL EDQRLKEERR RAAVEEKRRQ RLEEDKERHE AVVRRTMERS 150
    QKPRQKSNRW SWGSPLHGSS SIHSGDPDRR SVSTMNLSKH VDPVISKRLS 200
    SSSATLLNSP DRARRLQLSP WESSVVSRLL TPTHSFLARS KSTAALSGDT 250
    ASCSPIIMPF KAAHSRNPVD RPKLFVTPPE GSARRRTIHG LASHKRERER 300
    EHVPFHVSPG ARRTLSPSNL KARSPAPARL WLPSKSMPHL PGTPRPASSL 350
    PPGSVRAASA QAPSSSPGNI RPFKREVKVE PEKKDPLPAV KSRVPLVKVE 400
    EVTVEEGTPV KPPEPAAPAS APIATPAPAP ATDPAPVPAP SSTVTVGVVP 450
    KTSAGTTDPE EATRLLAEKR RLAREQREKE ERERKEKEEL ERQKIEELAR 500
    RVAEERSRRE EEARRLEEEQ AREKEELALR LAEEERERWE REEVERVQKQ 550
    KEEEARAREE AERARQEREK HFQKEEQERL ERKKRLEEIM RRTRRTETAD 600
    KKTTEQRNGD IAKGVLTGEP EVPALPCMAS SGNGESAESP HGVALQQSEV 650
    TTESSPDLEK QPNENGMSIQ NENFEEVINL PVGSKASRLD VTNENPEIPL 700
    KPILAFNDEG TLGPLPQVDG VQTQQTAEVI 730
    Length:730
    Mass (Da):82,022
    Last modified:November 1, 1998 - v1
    Checksum:iF44B4201DE8F5A17
    GO
    Isoform 2 (identifier: O88735-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         250-250: T → TVIPICPRS

    Note: No experimental confirmation available.

    Show »
    Length:738
    Mass (Da):82,888
    Checksum:i8CEAE46EE589C5EE
    GO

    Sequence cautioni

    The sequence AAH42771.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41Q → H in BAE21585. (PubMed:16141072)Curated
    Sequence conflicti8 – 81G → C in BAE21585. (PubMed:16141072)Curated
    Sequence conflicti16 – 161G → S in BAE21585. (PubMed:16141072)Curated
    Sequence conflicti35 – 351A → G in BAE21585. (PubMed:16141072)Curated
    Sequence conflicti360 – 3601A → T in AAH42771. (PubMed:15489334)Curated
    Sequence conflicti373 – 3731F → V in BAE21585. (PubMed:16141072)Curated
    Sequence conflicti373 – 3731F → V in AAH52637. (PubMed:15489334)Curated
    Sequence conflicti373 – 3731F → V in AAH42771. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei250 – 2501T → TVIPICPRS in isoform 2. 1 PublicationVSP_021318

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y15197 mRNA. Translation: CAA75495.1.
    AB098611 mRNA. Translation: BAC53729.1.
    AK133267 mRNA. Translation: BAE21585.1.
    BC042771 mRNA. Translation: AAH42771.1. Sequence problems.
    BC052637 mRNA. Translation: AAH52637.1.
    CCDSiCCDS56694.1. [O88735-2]
    RefSeqiNP_001185564.1. NM_001198635.1.
    NP_032661.2. NM_008635.2.
    UniGeneiMm.20928.

    Genome annotation databases

    GeneIDi17761.
    KEGGimmu:17761.
    UCSCiuc007eno.2. mouse. [O88735-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y15197 mRNA. Translation: CAA75495.1 .
    AB098611 mRNA. Translation: BAC53729.1 .
    AK133267 mRNA. Translation: BAE21585.1 .
    BC042771 mRNA. Translation: AAH42771.1 . Sequence problems.
    BC052637 mRNA. Translation: AAH52637.1 .
    CCDSi CCDS56694.1. [O88735-2 ]
    RefSeqi NP_001185564.1. NM_001198635.1.
    NP_032661.2. NM_008635.2.
    UniGenei Mm.20928.

    3D structure databases

    ProteinModelPortali O88735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O88735. 1 interaction.
    MINTi MINT-4105617.

    PTM databases

    PhosphoSitei O88735.

    Proteomic databases

    MaxQBi O88735.
    PaxDbi O88735.
    PRIDEi O88735.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17761.
    KEGGi mmu:17761.
    UCSCi uc007eno.2. mouse. [O88735-1 ]

    Organism-specific databases

    CTDi 9053.
    MGIi MGI:1328328. Map7.

    Phylogenomic databases

    eggNOGi NOG46082.
    HOGENOMi HOG000231569.
    HOVERGENi HBG081952.
    KOi K10433.
    PhylomeDBi O88735.

    Miscellaneous databases

    NextBioi 292449.
    PROi O88735.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_MTAP7.
    Genevestigatori O88735.

    Family and domain databases

    InterProi IPR008604. MAP7.
    [Graphical view ]
    PANTHERi PTHR15073. PTHR15073. 1 hit.
    Pfami PF05672. MAP7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The distribution of murine 115-kDa epithelial microtubule-associated protein (E-MAP-115) during embryogenesis and in adult organs suggests a role in epithelial polarization and differentiation."
      Fabre-Jonca N., Allaman J.-M., Radlgruber G., Meda P., Kiss J.Z., French L.E., Masson D.
      Differentiation 63:169-180(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6 X CBA.
    2. "Microtubule-associated protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4)."
      Suzuki M., Hirao A., Mizuno A.
      J. Biol. Chem. 278:51448-51453(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH TRPV4.
      Tissue: Kidney.
    3. Erratum
      Suzuki M., Hirao A., Mizuno A.
      J. Biol. Chem. 280:25948-25948(2005)
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Testis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-494 (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Eye and Mammary gland.
    6. "Microtubule-associated epithelial protein E-MAP-115 is localized in the spermatid manchette."
      Penttilae T.-L., Parvinen M., Paranko J.
      Int. J. Androl. 26:166-174(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMAP7_MOUSE
    AccessioniPrimary (citable) accession number: O88735
    Secondary accession number(s): Q3V0B9, Q7TQL9, Q80V60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3