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Protein

C-terminal-binding protein 1

Gene

Ctbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.5 Publications

Cofactori

NAD+By similarityNote: Cofactor binding induces a conformational change.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei204NADBy similarity1
Active sitei266By similarity1
Binding sitei290NADBy similarity1
Active sitei295By similarity1
Active sitei315Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 185NADBy similarity6
Nucleotide bindingi237 – 243NADBy similarity7
Nucleotide bindingi264 – 266NADBy similarity3
Nucleotide bindingi315 – 318NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 1 (EC:1.1.1.-)
Short name:
CtBP1
Gene namesi
Name:Ctbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1201685. Ctbp1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcriptional repressor complex Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760421 – 441C-terminal-binding protein 1Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Cross-linki429Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

ADP-ribosylated; when cells are exposed to brefeldin A.By similarity
The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-423 induces proteasomal degradation (By similarity).By similarity
Sumoylation on Lys-429 is promoted by the E3 SUMO-protein ligase CBX4.By similarity

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO88712.
PaxDbiO88712.
PeptideAtlasiO88712.
PRIDEiO88712.

PTM databases

iPTMnetiO88712.
PhosphoSitePlusiO88712.

Expressioni

Tissue specificityi

Expressed in a wide range of adult tissues.1 Publication

Developmental stagei

Expressed throughout the developmental stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000037373.
CleanExiMM_CTBP1.
ExpressionAtlasiO88712. baseline and differential.
GenevisibleiO88712. MM.

Interactioni

Subunit structurei

Homo- or heterodimer. Heterodimer with CTBP2. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif). Interacts with PNN, MECOM, ZNF366 and ZFHX1B. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, HIPK2, FOXP1, FOXP2, HDAC4, HDAC5, HDAC9, NRIP1, WIZ and ZNF217. Interacts with BCL6; the interaction is required for BCL6 transcriptional autoinhibition and inhibition of some BCL6 target genes. Interacts with IKZF4. Interacts with MCRIP1 (unphosphorylated form, via the PXDLS motif); competitively inhibiting CTBP-ZEB1 interaction (By similarity).By similarity9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HIC1Q1452610EBI-604547,EBI-2507362From a different organism.
Zeb1Q643184EBI-604547,EBI-8560245

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198961. 14 interactors.
DIPiDIP-33907N.
IntActiO88712. 10 interactors.
MINTiMINT-146962.
STRINGi10090.ENSMUSP00000078682.

Structurei

3D structure databases

ProteinModelPortaliO88712.
SMRiO88712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 70Interaction with GLIS2 1Add BLAST70
Regioni288 – 360Interaction with GLIS2 2Add BLAST73

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOVERGENiHBG001898.
InParanoidiO88712.
KOiK04496.
OrthoDBiEOG091G08GS.
PhylomeDBiO88712.
TreeFamiTF313593.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88712-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSHLLNKG LPLGVRPPIM NGPMHPRPLV ALLDGRDCTV EMPILKDVAT
60 70 80 90 100
VAFCDAQSTQ EIHEKVLNEA VGALMYHTIT LTREDLEKFK ALRIIVRIGS
110 120 130 140 150
GFDNIDIKSA GDLGIAVCNV PAASVEETAD STLCHILNLY RRTTWLHQAL
160 170 180 190 200
REGTRVQSVE QIREVASGAA RIRGETLGII GLGRVGQAVA LRAKAFGFNV
210 220 230 240 250
LFYDPYLSDG IERALGLQRV STLQDLLFHS DCVTLHCGLN EHNHHLINDF
260 270 280 290 300
TVKQMRQGAF LVNTARGGLV DEKALAQALK EGRIRGAALD VHESEPFSFS
310 320 330 340 350
QGPLKDAPNL ICTPHAAWYS EQASIEMREE AAREIRRAIT GRIPDSLKNC
360 370 380 390 400
VNKDHLTAAT HWASMDPAVV HPELNGAAYS RYPPGVVSVA PTGIPAAVEG
410 420 430 440
IVPSAMSLSH GLPPVAHPPH APSPGQTVKP EADRDHTSDQ L
Length:441
Mass (Da):47,745
Last modified:May 1, 2007 - v2
Checksum:i2105CC8D69D915F4
GO
Isoform 2 (identifier: O88712-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MGSSHLLNKGLPL → MS

Show »
Length:430
Mass (Da):46,614
Checksum:i0A36DBF66E6A8605
GO
Isoform 3 (identifier: O88712-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):39,848
Checksum:i0E71CBDFDBF2EDA9
GO

Sequence cautioni

The sequence BAE41586 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55D → G in BAE41586 (PubMed:16141072).Curated1
Sequence conflicti55D → G in BAE42587 (PubMed:16141072).Curated1
Sequence conflicti108K → R in BAE35946 (PubMed:16141072).Curated1
Sequence conflicti380Missing in CAA09219 (PubMed:10369679).Curated1
Sequence conflicti380Missing in BAE35946 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0247371 – 74Missing in isoform 3. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_0247381 – 13MGSSH…KGLPL → MS in isoform 2. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010483 mRNA. Translation: CAA09219.1.
AB033122 mRNA. Translation: BAA85180.1.
AK133816 mRNA. Translation: BAE21859.1.
AK160658 mRNA. Translation: BAE35946.1.
AK165276 mRNA. Translation: BAE38115.1.
AK170133 mRNA. Translation: BAE41586.1. Different initiation.
AK171650 mRNA. Translation: BAE42587.1.
BC013702 mRNA. Translation: AAH13702.1.
BC015071 mRNA. Translation: AAH15071.1.
BC042425 mRNA. Translation: AAH42425.1.
CCDSiCCDS19201.1. [O88712-1]
CCDS80255.1. [O88712-3]
RefSeqiNP_001185788.1. NM_001198859.1.
NP_001185789.1. NM_001198860.1.
NP_001185790.1. NM_001198861.1. [O88712-2]
NP_001297464.1. NM_001310535.1. [O88712-3]
NP_038530.1. NM_013502.3. [O88712-1]
UniGeneiMm.240076.
Mm.7286.

Genome annotation databases

EnsembliENSMUST00000079746; ENSMUSP00000078682; ENSMUSG00000037373. [O88712-1]
ENSMUST00000201575; ENSMUSP00000144554; ENSMUSG00000037373. [O88712-3]
GeneIDi13016.
KEGGimmu:13016.
UCSCiuc008xaj.2. mouse. [O88712-1]
uc008xak.2. mouse. [O88712-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010483 mRNA. Translation: CAA09219.1.
AB033122 mRNA. Translation: BAA85180.1.
AK133816 mRNA. Translation: BAE21859.1.
AK160658 mRNA. Translation: BAE35946.1.
AK165276 mRNA. Translation: BAE38115.1.
AK170133 mRNA. Translation: BAE41586.1. Different initiation.
AK171650 mRNA. Translation: BAE42587.1.
BC013702 mRNA. Translation: AAH13702.1.
BC015071 mRNA. Translation: AAH15071.1.
BC042425 mRNA. Translation: AAH42425.1.
CCDSiCCDS19201.1. [O88712-1]
CCDS80255.1. [O88712-3]
RefSeqiNP_001185788.1. NM_001198859.1.
NP_001185789.1. NM_001198860.1.
NP_001185790.1. NM_001198861.1. [O88712-2]
NP_001297464.1. NM_001310535.1. [O88712-3]
NP_038530.1. NM_013502.3. [O88712-1]
UniGeneiMm.240076.
Mm.7286.

3D structure databases

ProteinModelPortaliO88712.
SMRiO88712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198961. 14 interactors.
DIPiDIP-33907N.
IntActiO88712. 10 interactors.
MINTiMINT-146962.
STRINGi10090.ENSMUSP00000078682.

PTM databases

iPTMnetiO88712.
PhosphoSitePlusiO88712.

Proteomic databases

MaxQBiO88712.
PaxDbiO88712.
PeptideAtlasiO88712.
PRIDEiO88712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079746; ENSMUSP00000078682; ENSMUSG00000037373. [O88712-1]
ENSMUST00000201575; ENSMUSP00000144554; ENSMUSG00000037373. [O88712-3]
GeneIDi13016.
KEGGimmu:13016.
UCSCiuc008xaj.2. mouse. [O88712-1]
uc008xak.2. mouse. [O88712-2]

Organism-specific databases

CTDi1487.
MGIiMGI:1201685. Ctbp1.

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOVERGENiHBG001898.
InParanoidiO88712.
KOiK04496.
OrthoDBiEOG091G08GS.
PhylomeDBiO88712.
TreeFamiTF313593.

Enzyme and pathway databases

ReactomeiR-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Miscellaneous databases

ChiTaRSiCtbp1. mouse.
PROiO88712.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037373.
CleanExiMM_CTBP1.
ExpressionAtlasiO88712. baseline and differential.
GenevisibleiO88712. MM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTBP1_MOUSE
AccessioniPrimary (citable) accession number: O88712
Secondary accession number(s): Q3TAT1
, Q3TDL5, Q3TUM5, Q91WI6, Q91YX3, Q9QYG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.