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Protein

Origin recognition complex subunit 4

Gene

Orc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA replication origin binding Source: MGI
  3. nucleotide binding Source: MGI

GO - Biological processi

  1. DNA replication Source: MGI
  2. DNA replication initiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_247969. Assembly of the ORC complex at the origin of replication.
REACT_254983. Activation of the pre-replicative complex.
REACT_259974. CDC6 association with the ORC:origin complex.
REACT_262002. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 4
Gene namesi
Name:Orc4
Synonyms:Orc4l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1347043. Orc4.

Subcellular locationi

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. cytoplasm Source: MGI
  3. nuclear origin of replication recognition complex Source: UniProtKB
  4. nucleolus Source: MGI
  5. nucleoplasm Source: MGI
  6. nucleus Source: MGI
  7. origin recognition complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Origin recognition complex subunit 4PRO_0000127088Add
BLAST

Proteomic databases

MaxQBiO88708.
PRIDEiO88708.

PTM databases

PhosphoSiteiO88708.

Expressioni

Gene expression databases

BgeeiO88708.
ExpressionAtlasiO88708. baseline and differential.
GenevestigatoriO88708.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity). Interacts with DBF4.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliO88708.
SMRiO88708. Positions 58-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC4 family.Curated

Phylogenomic databases

eggNOGiNOG289784.
GeneTreeiENSGT00390000016542.
HOGENOMiHOG000007226.
HOVERGENiHBG000253.
InParanoidiO88708.
KOiK02606.
OMAiFQKFIQR.
OrthoDBiEOG72NRPZ.
TreeFamiTF101094.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF007858. ORC4. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O88708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKTKSNA HAECLSQVQR ILRERFCHHS PHSNLFGVQV QYKHLIELLK
60 70 80 90 100
RTAIYGESNS VLIVGPRGSG KTTLLNHALK ELMEIEVSEN VIQVHLNGLL
110 120 130 140 150
QTNEKIALKE ITRQLNLDNV VEDKVFGSFA ENLSFLLEAL QKGDRTSSCP
160 170 180 190 200
VIFILDEFDI FAHQKNQTLL YNLFDISQSA QTPVAVIGLT CRLDILELLE
210 220 230 240 250
KRVKSRFSHR QIHLMNSFDF PQYLKIFKEQ LSLPAEFPDK AFAERWNENV
260 270 280 290 300
HCLSEDSTVL EVLQKHFSVN KNLQSLHMLL MLALNRVTVS HPFMTSADLM
310 320 330 340 350
EAQHMCSLDS KANIVHGLSV LEICLIIAMK HLNDIYEEEP FNFQMVYNEF
360 370 380 390 400
QKFIQRKAHS VYNFEKPVVM KAFEHLQQLE LIKPVERTSV NSQREYQLVK
410 420 430
LLLDNTQIMN ALQKYSNCPT DVRQWATSSL SWL
Length:433
Mass (Da):49,982
Last modified:July 27, 2011 - v2
Checksum:i9857AB8CCF41982D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti141 – 1411Q → K in CAA76188 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16386 mRNA. Translation: CAA76188.1.
AJ003140 mRNA. Translation: CAA05896.1.
AK010258 mRNA. Translation: BAB26801.1.
AK147823 mRNA. Translation: BAE28161.1.
AK169291 mRNA. Translation: BAE41048.1.
AL732317 Genomic DNA. Translation: CAM14876.1.
CH466519 Genomic DNA. Translation: EDL26871.1.
BC015072 mRNA. Translation: AAH15072.1.
BC019748 mRNA. Translation: AAH19748.1.
CCDSiCCDS16022.1.
RefSeqiNP_036088.3. NM_011958.3.
XP_006498126.1. XM_006498063.1.
XP_006498127.1. XM_006498064.1.
XP_006498128.1. XM_006498065.1.
UniGeneiMm.291059.
Mm.314338.

Genome annotation databases

EnsembliENSMUST00000028098; ENSMUSP00000028098; ENSMUSG00000026761.
GeneIDi26428.
KEGGimmu:26428.
UCSCiuc008jpp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16386 mRNA. Translation: CAA76188.1.
AJ003140 mRNA. Translation: CAA05896.1.
AK010258 mRNA. Translation: BAB26801.1.
AK147823 mRNA. Translation: BAE28161.1.
AK169291 mRNA. Translation: BAE41048.1.
AL732317 Genomic DNA. Translation: CAM14876.1.
CH466519 Genomic DNA. Translation: EDL26871.1.
BC015072 mRNA. Translation: AAH15072.1.
BC019748 mRNA. Translation: AAH19748.1.
CCDSiCCDS16022.1.
RefSeqiNP_036088.3. NM_011958.3.
XP_006498126.1. XM_006498063.1.
XP_006498127.1. XM_006498064.1.
XP_006498128.1. XM_006498065.1.
UniGeneiMm.291059.
Mm.314338.

3D structure databases

ProteinModelPortaliO88708.
SMRiO88708. Positions 58-212.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO88708.

Proteomic databases

MaxQBiO88708.
PRIDEiO88708.

Protocols and materials databases

DNASUi26428.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028098; ENSMUSP00000028098; ENSMUSG00000026761.
GeneIDi26428.
KEGGimmu:26428.
UCSCiuc008jpp.2. mouse.

Organism-specific databases

CTDi5000.
MGIiMGI:1347043. Orc4.

Phylogenomic databases

eggNOGiNOG289784.
GeneTreeiENSGT00390000016542.
HOGENOMiHOG000007226.
HOVERGENiHBG000253.
InParanoidiO88708.
KOiK02606.
OMAiFQKFIQR.
OrthoDBiEOG72NRPZ.
TreeFamiTF101094.

Enzyme and pathway databases

ReactomeiREACT_204269. E2F-enabled inhibition of pre-replication complex formation.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_244558. CDT1 association with the CDC6:ORC:origin complex.
REACT_245230. Orc1 removal from chromatin.
REACT_247969. Assembly of the ORC complex at the origin of replication.
REACT_254983. Activation of the pre-replicative complex.
REACT_259974. CDC6 association with the ORC:origin complex.
REACT_262002. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

NextBioi304469.
PROiO88708.
SOURCEiSearch...

Gene expression databases

BgeeiO88708.
ExpressionAtlasiO88708. baseline and differential.
GenevestigatoriO88708.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF007858. ORC4. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex."
    Komrskova T., Yang H., Gavin K., Pappin P., Canas B., Kobayashi R., Hunt T., Stillman B.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and characterization of MmORC4 and MmORC5, two subunits of the mouse origin of replication recognition complex."
    Springer J., Kneissl M., Putter V., Grummt F.
    Chromosoma 108:243-249(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N and FVB/N-3.
    Tissue: Liver and Mammary tumor.
  7. "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells."
    Kneissl M., Puetter V., Szalay A.A., Grummt F.
    J. Mol. Biol. 327:111-128(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DBF4.

Entry informationi

Entry nameiORC4_MOUSE
AccessioniPrimary (citable) accession number: O88708
Secondary accession number(s): Q91V62, Q9QYX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.