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O88697

- STK16_MOUSE

UniProt

O88697 - STK16_MOUSE

Protein

Serine/threonine-protein kinase 16

Gene

Stk16

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates By similarity. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei148 – 1481Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl

    GO - Biological processi

    1. cellular response to transforming growth factor beta stimulus Source: Ensembl
    2. protein autophosphorylation Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 16 (EC:2.7.11.1)
    Alternative name(s):
    Embryo-derived protein kinase
    Short name:
    Edpk
    Myristoylated and palmitoylated serine/threonine-protein kinase
    Short name:
    MPSK
    Protein kinase Krct
    Protein kinase PKL12
    TGF-beta-stimulated factor 1
    Short name:
    TSF-1
    Tyrosine-protein kinase STK16 (EC:2.7.10.2)
    Gene namesi
    Name:Stk16
    Synonyms:Edpk, Krct, Mpsk1, Pkl12, Tsf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1313271. Stk16.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication. Membrane By similarity; Lipid-anchor By similarity
    Note: Associates with Golgi and Golgi-derived vesicles.

    GO - Cellular componenti

    1. Golgi-associated vesicle Source: UniProtKB
    2. membrane Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 305304Serine/threonine-protein kinase 16PRO_0000086702Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi6 – 61S-palmitoyl cysteineBy similarity
    Lipidationi8 – 81S-palmitoyl cysteineBy similarity
    Modified residuei197 – 1971Phosphoserine; by autocatalysisBy similarity
    Modified residuei198 – 1981Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiO88697.
    PaxDbiO88697.
    PRIDEiO88697.

    PTM databases

    PhosphoSiteiO88697.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed at low levels. Relatively higher levels in testis, kidney and liver.2 Publications

    Developmental stagei

    Expressed at all stages of developing embryo.1 Publication

    Gene expression databases

    ArrayExpressiO88697.
    BgeeiO88697.
    CleanExiMM_STK16.
    GenevestigatoriO88697.

    Interactioni

    Subunit structurei

    Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.By similarity

    Protein-protein interaction databases

    MINTiMINT-224631.
    STRINGi10090.ENSMUSP00000027401.

    Structurei

    3D structure databases

    ProteinModelPortaliO88697.
    SMRiO88697. Positions 13-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 293274Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 20237Activation loopBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075037.
    HOGENOMiHOG000157694.
    HOVERGENiHBG054359.
    InParanoidiQ3UEG5.
    KOiK08856.
    OMAiTEDQILW.
    OrthoDBiEOG7Z3F55.
    PhylomeDBiO88697.
    TreeFamiTF350433.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR    50
    ILCHEQQDQE EAQREAEMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF 100
    FKKGTLWNEI ERLKDQGSFL TEDQILPLLL GISRGLEAIH AKGYAHRDLK 150
    PTNILLGDEG QPVLMDLGSM NQACIQVEGS RQALALQDWA AQRCTISYRA 200
    PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ 250
    NELSIPQSPR HSSALRQLLS SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ 300
    HTTQI 305
    Length:305
    Mass (Da):34,382
    Last modified:January 23, 2007 - v3
    Checksum:iEB4725D26B210537
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 284KLGE → EIGG in BAA89662. (PubMed:10947953)Curated
    Sequence conflicti25 – 284KLGE → EIGG in BAA89499. (PubMed:16141072)Curated
    Sequence conflicti195 – 1951T → A in BAA89499. (PubMed:16141072)Curated
    Sequence conflicti267 – 2682QL → HV in AAD02811. (PubMed:10364453)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005790 mRNA. Translation: CAA06699.1.
    AB017197 mRNA. Translation: BAA74457.1.
    AF089869 mRNA. Translation: AAC98502.1.
    AF062076 mRNA. Translation: AAD02811.1.
    AB022285 Genomic DNA. Translation: BAA89662.1.
    AB022179 mRNA. Translation: BAA89499.1.
    AK149539 mRNA. Translation: BAE28946.1.
    CH466548 Genomic DNA. Translation: EDL00383.1.
    CH466548 Genomic DNA. Translation: EDL00384.1.
    CH466548 Genomic DNA. Translation: EDL00386.1.
    BC028999 mRNA. Translation: AAH28999.1.
    CCDSiCCDS15067.1.
    RefSeqiNP_001264921.1. NM_001277992.1.
    NP_035624.3. NM_011494.5.
    XP_006496524.1. XM_006496461.1.
    UniGeneiMm.1155.
    Mm.17461.

    Genome annotation databases

    EnsembliENSMUST00000027401; ENSMUSP00000027401; ENSMUSG00000026201.
    GeneIDi20872.
    KEGGimmu:20872.
    UCSCiuc007boh.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005790 mRNA. Translation: CAA06699.1 .
    AB017197 mRNA. Translation: BAA74457.1 .
    AF089869 mRNA. Translation: AAC98502.1 .
    AF062076 mRNA. Translation: AAD02811.1 .
    AB022285 Genomic DNA. Translation: BAA89662.1 .
    AB022179 mRNA. Translation: BAA89499.1 .
    AK149539 mRNA. Translation: BAE28946.1 .
    CH466548 Genomic DNA. Translation: EDL00383.1 .
    CH466548 Genomic DNA. Translation: EDL00384.1 .
    CH466548 Genomic DNA. Translation: EDL00386.1 .
    BC028999 mRNA. Translation: AAH28999.1 .
    CCDSi CCDS15067.1.
    RefSeqi NP_001264921.1. NM_001277992.1.
    NP_035624.3. NM_011494.5.
    XP_006496524.1. XM_006496461.1.
    UniGenei Mm.1155.
    Mm.17461.

    3D structure databases

    ProteinModelPortali O88697.
    SMRi O88697. Positions 13-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-224631.
    STRINGi 10090.ENSMUSP00000027401.

    PTM databases

    PhosphoSitei O88697.

    Proteomic databases

    MaxQBi O88697.
    PaxDbi O88697.
    PRIDEi O88697.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027401 ; ENSMUSP00000027401 ; ENSMUSG00000026201 .
    GeneIDi 20872.
    KEGGi mmu:20872.
    UCSCi uc007boh.2. mouse.

    Organism-specific databases

    CTDi 8576.
    MGIi MGI:1313271. Stk16.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075037.
    HOGENOMi HOG000157694.
    HOVERGENi HBG054359.
    InParanoidi Q3UEG5.
    KOi K08856.
    OMAi TEDQILW.
    OrthoDBi EOG7Z3F55.
    PhylomeDBi O88697.
    TreeFami TF350433.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 3474.

    Miscellaneous databases

    NextBioi 299715.
    PROi O88697.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88697.
    Bgeei O88697.
    CleanExi MM_STK16.
    Genevestigatori O88697.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression analysis, and functional characterization of PKL12, a member of a new subfamily of ser/thr kinases."
      Ligos J.M., Gerwin N., Fernandez P., Gutierrez-Ramos J.-C., Bernad A.
      Biochem. Biophys. Res. Commun. 249:380-384(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    2. "Molecular cloning and characterization of a novel protein serine/threonine kinase highly expressed in mouse embryo."
      Kurioka K., Nakagawa K., Denda K., Miyazawa K., Kitamura N.
      Biochim. Biophys. Acta 1443:275-284(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
      Tissue: Liver.
    3. "Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases."
      Stairs D.B., Perry Gardner H., Ha S.I., Copeland N.G., Gilbert D.J., Jenkins N.A., Chodosh L.A.
      Hum. Mol. Genet. 7:2157-2166(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: FVB.
      Tissue: Mammary gland.
    4. "Identification and characterization of a myristylated and palmitylated serine/threonine protein kinase."
      Berson A.E., Young C., Morrison S.L., Fujii G.H., Sheung J., Wu B., Bolen J.B., Burkhardt A.L.
      Biochem. Biophys. Res. Commun. 259:533-538(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway."
      Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K.
      Biochem. J. 350:395-404(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ.
    6. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver.
    7. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiSTK16_MOUSE
    AccessioniPrimary (citable) accession number: O88697
    Secondary accession number(s): Q3UEG5
    , Q9JMJ0, Q9JMJ1, Q9QX00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3