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O88697

- STK16_MOUSE

UniProt

O88697 - STK16_MOUSE

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Protein
Serine/threonine-protein kinase 16
Gene
Stk16, Edpk, Krct, Mpsk1, Pkl12, Tsf1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates By similarity. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATP By similarity
Active sitei148 – 1481Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to transforming growth factor beta stimulus Source: Ensembl
  2. protein autophosphorylation Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 16 (EC:2.7.11.1)
Alternative name(s):
Embryo-derived protein kinase
Short name:
Edpk
Myristoylated and palmitoylated serine/threonine-protein kinase
Short name:
MPSK
Protein kinase Krct
Protein kinase PKL12
TGF-beta-stimulated factor 1
Short name:
TSF-1
Tyrosine-protein kinase STK16 (EC:2.7.10.2)
Gene namesi
Name:Stk16
Synonyms:Edpk, Krct, Mpsk1, Pkl12, Tsf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1313271. Stk16.

Subcellular locationi

Cytoplasmperinuclear region. Membrane; Lipid-anchor By similarity
Note: Associates with Golgi and Golgi-derived vesicles.1 Publication

GO - Cellular componenti

  1. Golgi-associated vesicle Source: UniProtKB
  2. membrane Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 305304Serine/threonine-protein kinase 16
PRO_0000086702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Lipidationi6 – 61S-palmitoyl cysteine By similarity
Lipidationi8 – 81S-palmitoyl cysteine By similarity
Modified residuei197 – 1971Phosphoserine; by autocatalysis By similarity
Modified residuei198 – 1981Phosphotyrosine; by autocatalysis By similarity

Post-translational modificationi

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 By similarity.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiO88697.
PaxDbiO88697.
PRIDEiO88697.

PTM databases

PhosphoSiteiO88697.

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels. Relatively higher levels in testis, kidney and liver.2 Publications

Developmental stagei

Expressed at all stages of developing embryo.1 Publication

Gene expression databases

ArrayExpressiO88697.
BgeeiO88697.
CleanExiMM_STK16.
GenevestigatoriO88697.

Interactioni

Subunit structurei

Monomer By similarity. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1 By similarity.

Protein-protein interaction databases

MINTiMINT-224631.
STRINGi10090.ENSMUSP00000027401.

Structurei

3D structure databases

ProteinModelPortaliO88697.
SMRiO88697. Positions 13-299.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 293274Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 20237Activation loop By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075037.
HOGENOMiHOG000157694.
HOVERGENiHBG054359.
InParanoidiQ3UEG5.
KOiK08856.
OMAiTEDQILW.
OrthoDBiEOG7Z3F55.
PhylomeDBiO88697.
TreeFamiTF350433.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88697-1 [UniParc]FASTAAdd to Basket

« Hide

MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR    50
ILCHEQQDQE EAQREAEMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF 100
FKKGTLWNEI ERLKDQGSFL TEDQILPLLL GISRGLEAIH AKGYAHRDLK 150
PTNILLGDEG QPVLMDLGSM NQACIQVEGS RQALALQDWA AQRCTISYRA 200
PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ 250
NELSIPQSPR HSSALRQLLS SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ 300
HTTQI 305
Length:305
Mass (Da):34,382
Last modified:January 23, 2007 - v3
Checksum:iEB4725D26B210537
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 284KLGE → EIGG in BAA89662. 1 Publication
Sequence conflicti25 – 284KLGE → EIGG in BAA89499. 1 Publication
Sequence conflicti195 – 1951T → A in BAA89499. 1 Publication
Sequence conflicti267 – 2682QL → HV in AAD02811. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005790 mRNA. Translation: CAA06699.1.
AB017197 mRNA. Translation: BAA74457.1.
AF089869 mRNA. Translation: AAC98502.1.
AF062076 mRNA. Translation: AAD02811.1.
AB022285 Genomic DNA. Translation: BAA89662.1.
AB022179 mRNA. Translation: BAA89499.1.
AK149539 mRNA. Translation: BAE28946.1.
CH466548 Genomic DNA. Translation: EDL00383.1.
CH466548 Genomic DNA. Translation: EDL00384.1.
CH466548 Genomic DNA. Translation: EDL00386.1.
BC028999 mRNA. Translation: AAH28999.1.
CCDSiCCDS15067.1.
RefSeqiNP_001264921.1. NM_001277992.1.
NP_035624.3. NM_011494.5.
XP_006496524.1. XM_006496461.1.
UniGeneiMm.1155.
Mm.17461.

Genome annotation databases

EnsembliENSMUST00000027401; ENSMUSP00000027401; ENSMUSG00000026201.
GeneIDi20872.
KEGGimmu:20872.
UCSCiuc007boh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005790 mRNA. Translation: CAA06699.1 .
AB017197 mRNA. Translation: BAA74457.1 .
AF089869 mRNA. Translation: AAC98502.1 .
AF062076 mRNA. Translation: AAD02811.1 .
AB022285 Genomic DNA. Translation: BAA89662.1 .
AB022179 mRNA. Translation: BAA89499.1 .
AK149539 mRNA. Translation: BAE28946.1 .
CH466548 Genomic DNA. Translation: EDL00383.1 .
CH466548 Genomic DNA. Translation: EDL00384.1 .
CH466548 Genomic DNA. Translation: EDL00386.1 .
BC028999 mRNA. Translation: AAH28999.1 .
CCDSi CCDS15067.1.
RefSeqi NP_001264921.1. NM_001277992.1.
NP_035624.3. NM_011494.5.
XP_006496524.1. XM_006496461.1.
UniGenei Mm.1155.
Mm.17461.

3D structure databases

ProteinModelPortali O88697.
SMRi O88697. Positions 13-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-224631.
STRINGi 10090.ENSMUSP00000027401.

PTM databases

PhosphoSitei O88697.

Proteomic databases

MaxQBi O88697.
PaxDbi O88697.
PRIDEi O88697.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027401 ; ENSMUSP00000027401 ; ENSMUSG00000026201 .
GeneIDi 20872.
KEGGi mmu:20872.
UCSCi uc007boh.2. mouse.

Organism-specific databases

CTDi 8576.
MGIi MGI:1313271. Stk16.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000075037.
HOGENOMi HOG000157694.
HOVERGENi HBG054359.
InParanoidi Q3UEG5.
KOi K08856.
OMAi TEDQILW.
OrthoDBi EOG7Z3F55.
PhylomeDBi O88697.
TreeFami TF350433.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 3474.

Miscellaneous databases

NextBioi 299715.
PROi O88697.
SOURCEi Search...

Gene expression databases

ArrayExpressi O88697.
Bgeei O88697.
CleanExi MM_STK16.
Genevestigatori O88697.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression analysis, and functional characterization of PKL12, a member of a new subfamily of ser/thr kinases."
    Ligos J.M., Gerwin N., Fernandez P., Gutierrez-Ramos J.-C., Bernad A.
    Biochem. Biophys. Res. Commun. 249:380-384(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  2. "Molecular cloning and characterization of a novel protein serine/threonine kinase highly expressed in mouse embryo."
    Kurioka K., Nakagawa K., Denda K., Miyazawa K., Kitamura N.
    Biochim. Biophys. Acta 1443:275-284(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    Tissue: Liver.
  3. "Cloning and characterization of Krct, a member of a novel subfamily of serine/threonine kinases."
    Stairs D.B., Perry Gardner H., Ha S.I., Copeland N.G., Gilbert D.J., Jenkins N.A., Chodosh L.A.
    Hum. Mol. Genet. 7:2157-2166(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: FVB.
    Tissue: Mammary gland.
  4. "Identification and characterization of a myristylated and palmitylated serine/threonine protein kinase."
    Berson A.E., Young C., Morrison S.L., Fujii G.H., Sheung J., Wu B., Bolen J.B., Burkhardt A.L.
    Biochem. Biophys. Res. Commun. 259:533-538(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway."
    Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K.
    Biochem. J. 350:395-404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
  6. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  7. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiSTK16_MOUSE
AccessioniPrimary (citable) accession number: O88697
Secondary accession number(s): Q3UEG5
, Q9JMJ0, Q9JMJ1, Q9QX00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi