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Protein

ATP-dependent Clp protease proteolytic subunit, mitochondrial

Gene

Clpp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149NucleophileBy similarity1
Active sitei174By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.92. 3474.

Protein family/group databases

MEROPSiS14.003.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit, mitochondrial (EC:3.4.21.92)
Alternative name(s):
Endopeptidase Clp
Gene namesi
Name:Clpp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1858213. Clpp.

Subcellular locationi

GO - Cellular componenti

  • endopeptidase Clp complex Source: UniProtKB
  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Homozygous pups are born at about 60 % of the expected Mendelian rate, indicating decreased intrauterine survival. Mutant mice are smaller in size than wild-type littermates, show decreased motor activity, are completely deaf after 12 months and their lifespan is decreased relative to that of wild-type littermates. Both female and male mutant mice are completely infertile due to defects in germ cell development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52MitochondrionBy similarityAdd BLAST52
ChainiPRO_000000551753 – 272ATP-dependent Clp protease proteolytic subunit, mitochondrialAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196N6-succinyllysineCombined sources1
Modified residuei207N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88696.
MaxQBiO88696.
PaxDbiO88696.
PeptideAtlasiO88696.
PRIDEiO88696.

2D gel databases

REPRODUCTION-2DPAGEiIPI00133270.
O88696.

PTM databases

iPTMnetiO88696.
PhosphoSitePlusiO88696.

Expressioni

Tissue specificityi

Detected in liver (at protein level). High levels found in heart, liver and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSMUSG00000002660.
GenevisibleiO88696. MM.

Interactioni

Subunit structurei

Fourteen CLPP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity. Component of the Clp complex formed by the assembly of two CLPP heptameric rings with two CLPX hexameric rings, giving rise to a symmetrical structure with two central CLPP rings flanked by a CLPX ring at either end of the complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207517. 1 interactor.
IntActiO88696. 3 interactors.
MINTiMINT-1850742.
STRINGi10090.ENSMUSP00000002735.

Structurei

3D structure databases

ProteinModelPortaliO88696.
SMRiO88696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
GeneTreeiENSGT00390000005830.
HOGENOMiHOG000285833.
HOVERGENiHBG001689.
InParanoidiO88696.
KOiK01358.
OMAiLFLQSEN.
OrthoDBiEOG091G0KCO.
PhylomeDBiO88696.
TreeFamiTF105002.

Family and domain databases

HAMAPiMF_00444. ClpP. 1 hit.
InterProiView protein in InterPro
IPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiView protein in Pfam
PF00574. CLP_protease. 1 hit.
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiView protein in PROSITE
PS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWPRVLLGEA RVAVDGCRAL LSRLAVHFSP PWTAVSCSPL RRSLHGTATR
60 70 80 90 100
AFPLIPIVVE QTGRGERAYD IYSRLLRERI VCVMGPIDDS VASLVIAQLL
110 120 130 140 150
FLQSESNKKP IHMYINSPGG VVTAGLAIYD TMQYILNPIC TWCVGQAASM
160 170 180 190 200
GSLLLAAGSP GMRHSLPNSR IMIHQPSGGA RGQATDIAIQ AEEIMKLKKQ
210 220 230 240 250
LYNIYAKHTK QSLQVIESAM ERDRYMSPME AQEFGILDKV LVHPPQDGED
260 270
EPELVQKETA TAPTDPPAPT ST
Length:272
Mass (Da):29,800
Last modified:November 1, 1998 - v1
Checksum:i1F34C21E9C5C04D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005253 mRNA. Translation: CAA06443.1.
AJ012249
, AJ012250, AJ012251, AJ012252, AJ012253 Genomic DNA. Translation: CAA09966.1.
AK004024 mRNA. Translation: BAB23132.1.
AK145765 mRNA. Translation: BAE26636.1.
AK168053 mRNA. Translation: BAE40033.1.
BC001998 mRNA. Translation: AAH01998.1.
CCDSiCCDS28920.1.
RefSeqiNP_059089.1. NM_017393.2.
UniGeneiMm.287892.

Genome annotation databases

EnsembliENSMUST00000002735; ENSMUSP00000002735; ENSMUSG00000002660.
GeneIDi53895.
KEGGimmu:53895.
UCSCiuc008ddm.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCLPP_MOUSE
AccessioniPrimary (citable) accession number: O88696
Secondary accession number(s): Q3TI13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: June 7, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families