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O88693

- CEGT_MOUSE

UniProt

O88693 - CEGT_MOUSE

Protein

Ceramide glucosyltransferase

Gene

Ugcg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase" By similarity.By similarity

    Catalytic activityi

    UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei193 – 1931May play an important role in binding to the inhibitors DEPC and PDMPBy similarity

    GO - Molecular functioni

    1. ceramide glucosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_199008. Glycosphingolipid metabolism.
    UniPathwayiUPA00222.

    Protein family/group databases

    CAZyiGT21. Glycosyltransferase Family 21.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ceramide glucosyltransferase (EC:2.4.1.80)
    Alternative name(s):
    GLCT-1
    Glucosylceramide synthase
    Short name:
    GCS
    UDP-glucose ceramide glucosyltransferase
    UDP-glucose:N-acylsphingosine D-glucosyltransferase
    Gene namesi
    Name:Ugcg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1332243. Ugcg.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 394394Ceramide glucosyltransferasePRO_0000059177Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei117 – 1171N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiO88693.

    PTM databases

    PhosphoSiteiO88693.

    Expressioni

    Gene expression databases

    BgeeiO88693.
    CleanExiMM_UGCG.
    GenevestigatoriO88693.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliO88693.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010LumenalSequence Analysis
    Topological domaini33 – 195163CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini216 – 28772LumenalSequence AnalysisAdd
    BLAST
    Topological domaini305 – 3095CytoplasmicSequence Analysis
    Topological domaini329 – 34820LumenalSequence AnalysisAdd
    BLAST
    Topological domaini370 – 39425CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei288 – 30417HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 32819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1215.
    GeneTreeiENSGT00390000012898.
    HOGENOMiHOG000039663.
    HOVERGENiHBG003997.
    InParanoidiA2AN90.
    KOiK00720.
    OMAiLVWICDS.
    OrthoDBiEOG70KGPR.
    PhylomeDBiO88693.
    TreeFamiTF314564.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR025993. Ceramide_glucosylTrfase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF13506. Glyco_transf_21. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O88693-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLDLAQEG MALFGFVLFV VLWLMHFMSI IYTRLHLNKK ATDKQPYSKL    50
    PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK 100
    LLGKYPNVDA RLFIGGKKVG INPKINNLMP AYEVAKYDLI WICDSGIRVI 150
    PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRSYISA 200
    NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF 250
    SMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS 300
    LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA 350
    VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV 394
    Length:394
    Mass (Da):44,839
    Last modified:November 1, 1998 - v1
    Checksum:i8B18D09437CACE0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012807 Genomic DNA. Translation: BAA33558.1.
    D89866 mRNA. Translation: BAA28782.1.
    AL808112 Genomic DNA. Translation: CAM26180.1.
    CH466565 Genomic DNA. Translation: EDL02214.1.
    BC050828 mRNA. Translation: AAH50828.1.
    CCDSiCCDS18219.1.
    RefSeqiNP_035803.1. NM_011673.3.
    UniGeneiMm.198803.

    Genome annotation databases

    EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381.
    GeneIDi22234.
    KEGGimmu:22234.
    UCSCiuc008szr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012807 Genomic DNA. Translation: BAA33558.1 .
    D89866 mRNA. Translation: BAA28782.1 .
    AL808112 Genomic DNA. Translation: CAM26180.1 .
    CH466565 Genomic DNA. Translation: EDL02214.1 .
    BC050828 mRNA. Translation: AAH50828.1 .
    CCDSi CCDS18219.1.
    RefSeqi NP_035803.1. NM_011673.3.
    UniGenei Mm.198803.

    3D structure databases

    ProteinModelPortali O88693.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O88693.
    ChEMBLi CHEMBL6013.

    Protein family/group databases

    CAZyi GT21. Glycosyltransferase Family 21.

    PTM databases

    PhosphoSitei O88693.

    Proteomic databases

    PRIDEi O88693.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030074 ; ENSMUSP00000030074 ; ENSMUSG00000028381 .
    GeneIDi 22234.
    KEGGi mmu:22234.
    UCSCi uc008szr.1. mouse.

    Organism-specific databases

    CTDi 7357.
    MGIi MGI:1332243. Ugcg.

    Phylogenomic databases

    eggNOGi COG1215.
    GeneTreei ENSGT00390000012898.
    HOGENOMi HOG000039663.
    HOVERGENi HBG003997.
    InParanoidi A2AN90.
    KOi K00720.
    OMAi LVWICDS.
    OrthoDBi EOG70KGPR.
    PhylomeDBi O88693.
    TreeFami TF314564.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_199008. Glycosphingolipid metabolism.

    Miscellaneous databases

    ChiTaRSi UGCG. mouse.
    NextBioi 302281.
    PROi O88693.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88693.
    CleanExi MM_UGCG.
    Genevestigatori O88693.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR025993. Ceramide_glucosylTrfase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF13506. Glyco_transf_21. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the mouse ceramide glucosyltransferase gene."
      Ichikawa S., Ozawa K., Hirabayashi Y.
      Biochem. Biophys. Res. Commun. 253:707-711(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    2. "Molecular cloning and expression of mouse ceramide glucosyltransferase."
      Ichikawa S., Ozawa K., Hirabayashi Y.
      Biochem. Mol. Biol. Int. 44:1193-1202(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: LimbImported.
    6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiCEGT_MOUSE
    AccessioniPrimary (citable) accession number: O88693
    Secondary accession number(s): A2AN90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3