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Protein

Ceramide glucosyltransferase

Gene

Ugcg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase" (By similarity).By similarity

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.By similarity

Pathway: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei193 – 1931May play an important role in binding to the inhibitors DEPC and PDMPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.4.1.80. 3474.
ReactomeiREACT_339900. Glycosphingolipid metabolism.
UniPathwayiUPA00222.

Protein family/group databases

CAZyiGT21. Glycosyltransferase Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferase (EC:2.4.1.80)
Alternative name(s):
GLCT-1
Glucosylceramide synthase
Short name:
GCS
UDP-glucose ceramide glucosyltransferase
UDP-glucose:N-acylsphingosine D-glucosyltransferase
Gene namesi
Name:Ugcg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1332243. Ugcg.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010LumenalSequence Analysis
Transmembranei11 – 3222HelicalSequence AnalysisAdd
BLAST
Topological domaini33 – 195163CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei196 – 21520HelicalSequence AnalysisAdd
BLAST
Topological domaini216 – 28772LumenalSequence AnalysisAdd
BLAST
Transmembranei288 – 30417HelicalSequence AnalysisAdd
BLAST
Topological domaini305 – 3095CytoplasmicSequence Analysis
Transmembranei310 – 32819HelicalSequence AnalysisAdd
BLAST
Topological domaini329 – 34820LumenalSequence AnalysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence AnalysisAdd
BLAST
Topological domaini370 – 39425CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Ceramide glucosyltransferasePRO_0000059177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei117 – 1171N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO88693.
PRIDEiO88693.

PTM databases

PhosphoSiteiO88693.

Expressioni

Gene expression databases

BgeeiO88693.
CleanExiMM_UGCG.
GenevisibleiO88693. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030074.

Structurei

3D structure databases

ProteinModelPortaliO88693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000012898.
HOGENOMiHOG000039663.
HOVERGENiHBG003997.
InParanoidiO88693.
KOiK00720.
OMAiLVWICDS.
OrthoDBiEOG70KGPR.
PhylomeDBiO88693.
TreeFamiTF314564.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13506. Glyco_transf_21. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O88693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLDLAQEG MALFGFVLFV VLWLMHFMSI IYTRLHLNKK ATDKQPYSKL
60 70 80 90 100
PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK
110 120 130 140 150
LLGKYPNVDA RLFIGGKKVG INPKINNLMP AYEVAKYDLI WICDSGIRVI
160 170 180 190 200
PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRSYISA
210 220 230 240 250
NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF
260 270 280 290 300
SMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS
310 320 330 340 350
LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA
360 370 380 390
VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV
Length:394
Mass (Da):44,839
Last modified:November 1, 1998 - v1
Checksum:i8B18D09437CACE0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012807 Genomic DNA. Translation: BAA33558.1.
D89866 mRNA. Translation: BAA28782.1.
AL808112 Genomic DNA. Translation: CAM26180.1.
CH466565 Genomic DNA. Translation: EDL02214.1.
BC050828 mRNA. Translation: AAH50828.1.
CCDSiCCDS18219.1.
RefSeqiNP_035803.1. NM_011673.3.
UniGeneiMm.198803.

Genome annotation databases

EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381.
GeneIDi22234.
KEGGimmu:22234.
UCSCiuc008szr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012807 Genomic DNA. Translation: BAA33558.1.
D89866 mRNA. Translation: BAA28782.1.
AL808112 Genomic DNA. Translation: CAM26180.1.
CH466565 Genomic DNA. Translation: EDL02214.1.
BC050828 mRNA. Translation: AAH50828.1.
CCDSiCCDS18219.1.
RefSeqiNP_035803.1. NM_011673.3.
UniGeneiMm.198803.

3D structure databases

ProteinModelPortaliO88693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030074.

Chemistry

BindingDBiO88693.
ChEMBLiCHEMBL6013.

Protein family/group databases

CAZyiGT21. Glycosyltransferase Family 21.

PTM databases

PhosphoSiteiO88693.

Proteomic databases

MaxQBiO88693.
PRIDEiO88693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381.
GeneIDi22234.
KEGGimmu:22234.
UCSCiuc008szr.1. mouse.

Organism-specific databases

CTDi7357.
MGIiMGI:1332243. Ugcg.

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000012898.
HOGENOMiHOG000039663.
HOVERGENiHBG003997.
InParanoidiO88693.
KOiK00720.
OMAiLVWICDS.
OrthoDBiEOG70KGPR.
PhylomeDBiO88693.
TreeFamiTF314564.

Enzyme and pathway databases

UniPathwayiUPA00222.
BRENDAi2.4.1.80. 3474.
ReactomeiREACT_339900. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSiUgcg. mouse.
NextBioi302281.
PROiO88693.
SOURCEiSearch...

Gene expression databases

BgeeiO88693.
CleanExiMM_UGCG.
GenevisibleiO88693. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13506. Glyco_transf_21. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the mouse ceramide glucosyltransferase gene."
    Ichikawa S., Ozawa K., Hirabayashi Y.
    Biochem. Biophys. Res. Commun. 253:707-711(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "Molecular cloning and expression of mouse ceramide glucosyltransferase."
    Ichikawa S., Ozawa K., Hirabayashi Y.
    Biochem. Mol. Biol. Int. 44:1193-1202(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: LimbImported.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCEGT_MOUSE
AccessioniPrimary (citable) accession number: O88693
Secondary accession number(s): A2AN90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.