SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O88693

- CEGT_MOUSE

UniProt

O88693 - CEGT_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ceramide glucosyltransferase

Gene
Ugcg
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase" By similarity.

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei193 – 1931May play an important role in binding to the inhibitors DEPC and PDMP By similarity

GO - Molecular functioni

  1. ceramide glucosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. sphingolipid metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiREACT_199008. Glycosphingolipid metabolism.
UniPathwayiUPA00222.

Protein family/group databases

CAZyiGT21. Glycosyltransferase Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferase (EC:2.4.1.80)
Alternative name(s):
GLCT-1
Glucosylceramide synthase
Short name:
GCS
UDP-glucose ceramide glucosyltransferase
UDP-glucose:N-acylsphingosine D-glucosyltransferase
Gene namesi
Name:Ugcg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1332243. Ugcg.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Lumenal Reviewed prediction
Transmembranei11 – 3222Helical; Reviewed predictionAdd
BLAST
Topological domaini33 – 195163Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei196 – 21520Helical; Reviewed predictionAdd
BLAST
Topological domaini216 – 28772Lumenal Reviewed predictionAdd
BLAST
Transmembranei288 – 30417Helical; Reviewed predictionAdd
BLAST
Topological domaini305 – 3095Cytoplasmic Reviewed prediction
Transmembranei310 – 32819Helical; Reviewed predictionAdd
BLAST
Topological domaini329 – 34820Lumenal Reviewed predictionAdd
BLAST
Transmembranei349 – 36921Helical; Reviewed predictionAdd
BLAST
Topological domaini370 – 39425Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Ceramide glucosyltransferasePRO_0000059177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei117 – 1171N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO88693.

PTM databases

PhosphoSiteiO88693.

Expressioni

Gene expression databases

BgeeiO88693.
CleanExiMM_UGCG.
GenevestigatoriO88693.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliO88693.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000012898.
HOGENOMiHOG000039663.
HOVERGENiHBG003997.
InParanoidiA2AN90.
KOiK00720.
OMAiLVWICDS.
OrthoDBiEOG70KGPR.
PhylomeDBiO88693.
TreeFamiTF314564.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13506. Glyco_transf_21. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O88693-1 [UniParc]FASTAAdd to Basket

« Hide

MALLDLAQEG MALFGFVLFV VLWLMHFMSI IYTRLHLNKK ATDKQPYSKL    50
PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK 100
LLGKYPNVDA RLFIGGKKVG INPKINNLMP AYEVAKYDLI WICDSGIRVI 150
PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRSYISA 200
NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF 250
SMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS 300
LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA 350
VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV 394
Length:394
Mass (Da):44,839
Last modified:November 1, 1998 - v1
Checksum:i8B18D09437CACE0E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012807 Genomic DNA. Translation: BAA33558.1.
D89866 mRNA. Translation: BAA28782.1.
AL808112 Genomic DNA. Translation: CAM26180.1.
CH466565 Genomic DNA. Translation: EDL02214.1.
BC050828 mRNA. Translation: AAH50828.1.
CCDSiCCDS18219.1.
RefSeqiNP_035803.1. NM_011673.3.
UniGeneiMm.198803.

Genome annotation databases

EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381.
GeneIDi22234.
KEGGimmu:22234.
UCSCiuc008szr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB012807 Genomic DNA. Translation: BAA33558.1 .
D89866 mRNA. Translation: BAA28782.1 .
AL808112 Genomic DNA. Translation: CAM26180.1 .
CH466565 Genomic DNA. Translation: EDL02214.1 .
BC050828 mRNA. Translation: AAH50828.1 .
CCDSi CCDS18219.1.
RefSeqi NP_035803.1. NM_011673.3.
UniGenei Mm.198803.

3D structure databases

ProteinModelPortali O88693.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi O88693.
ChEMBLi CHEMBL6013.

Protein family/group databases

CAZyi GT21. Glycosyltransferase Family 21.

PTM databases

PhosphoSitei O88693.

Proteomic databases

PRIDEi O88693.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030074 ; ENSMUSP00000030074 ; ENSMUSG00000028381 .
GeneIDi 22234.
KEGGi mmu:22234.
UCSCi uc008szr.1. mouse.

Organism-specific databases

CTDi 7357.
MGIi MGI:1332243. Ugcg.

Phylogenomic databases

eggNOGi COG1215.
GeneTreei ENSGT00390000012898.
HOGENOMi HOG000039663.
HOVERGENi HBG003997.
InParanoidi A2AN90.
KOi K00720.
OMAi LVWICDS.
OrthoDBi EOG70KGPR.
PhylomeDBi O88693.
TreeFami TF314564.

Enzyme and pathway databases

UniPathwayi UPA00222 .
Reactomei REACT_199008. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSi UGCG. mouse.
NextBioi 302281.
PROi O88693.
SOURCEi Search...

Gene expression databases

Bgeei O88693.
CleanExi MM_UGCG.
Genevestigatori O88693.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF13506. Glyco_transf_21. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the mouse ceramide glucosyltransferase gene."
    Ichikawa S., Ozawa K., Hirabayashi Y.
    Biochem. Biophys. Res. Commun. 253:707-711(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "Molecular cloning and expression of mouse ceramide glucosyltransferase."
    Ichikawa S., Ozawa K., Hirabayashi Y.
    Biochem. Mol. Biol. Int. 44:1193-1202(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCEGT_MOUSE
AccessioniPrimary (citable) accession number: O88693
Secondary accession number(s): A2AN90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi