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Protein

Matrix metalloproteinase-23

Gene

Mmp23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by TIMP2.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi212Zinc; catalyticPROSITE-ProRule annotation1
Active sitei213PROSITE-ProRule annotation1
Metal bindingi216Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi222Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM10.022.
TCDBi8.B.14.2.1. the sea anemone peptide toxin, class 1 (bgk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-23 (EC:3.4.24.-)
Short name:
MMP-23
Alternative name(s):
CAMP metalloproteinase
Short name:
CA-MMP
Cleaved into the following chain:
Gene namesi
Name:Mmp23
Synonyms:Cammp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1347361. Mmp23.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Topological domaini40 – 391LumenalSequence analysisAdd BLAST352

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002599151 – 391Matrix metalloproteinase-23Add BLAST391
PropeptideiPRO_00002599161 – 79Sequence analysisAdd BLAST79
ChainiPRO_000025991780 – 391Matrix metalloproteinase-23, soluble formAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi256 ↔ 290By similarity
Disulfide bondi263 ↔ 283By similarity
Disulfide bondi272 ↔ 287By similarity
Glycosylationi317N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi322 ↔ 371By similarity

Post-translational modificationi

N-glycosylated.By similarity
Proteolytic cleavage might yield an active form.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei79 – 80Cleavage; by furin-like proteaseSequence analysis2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiO88676.
PaxDbiO88676.
PRIDEiO88676.

PTM databases

iPTMnetiO88676.
PhosphoSitePlusiO88676.

Expressioni

Tissue specificityi

Expressed at relatively high level in heart, lung and spleen. Not detected in brain, liver, skeletal muscle, kidney and testis.1 Publication

Developmental stagei

Expressed at relatively high level at 7 days old embryo compared to those at stadges day 11, 15 and 17.1 Publication

Gene expression databases

BgeeiENSMUSG00000029061.
CleanExiMM_MMP23.
GenevisibleiO88676. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030937.

Structurei

3D structure databases

ProteinModelPortaliO88676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 290ShKTPROSITE-ProRule annotationAdd BLAST35
Domaini298 – 383Ig-like C2-typeAdd BLAST86

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 79Poly-Arg4

Domaini

The ShKT domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4 (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00870000136399.
HOGENOMiHOG000231157.
HOVERGENiHBG053177.
InParanoidiO88676.
KOiK08001.
OMAiHCFDGIT.
OrthoDBiEOG091G03DP.
PhylomeDBiO88676.
TreeFamiTF315428.

Family and domain databases

CDDicd04278. ZnMc_MMP. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.40.390.10. 1 hit.
InterProiView protein in InterPro
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028687. MMP23.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
PANTHERiPTHR10201:SF202. PTHR10201:SF202. 1 hit.
PfamiView protein in Pfam
PF00413. Peptidase_M10. 1 hit.
PF01549. ShK. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiView protein in SMART
SM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.
PS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88676-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCRACLRPE ASGAVQGRWL GAALSGLCLL SALALLEWLG APTETAWRAA
60 70 80 90 100
QGNVDAPNVG SSTAQVPRLL TMSVTRRRRY TLTPARLRWD HFNLTYRVLS
110 120 130 140 150
FPRNLLSPEE TRRGLAAAFR MWSDVSPFSF REVAPERPSD LKIGFYPVNH
160 170 180 190 200
TDCLVSAVHH CFDGPTGELA HAFFPPHGGI HFDDSEYWVL GPTRYSWKKG
210 220 230 240 250
VWLTNLVHVA AHEIGHALGL MHSQQDQALM HLNATLRGWK ALSQDELWGL
260 270 280 290 300
HRLYGCLDRI FVCASWARKG FCDVRQRLMK RLCPRSCDFC YEFPFPTVAT
310 320 330 340 350
TTSPTRTKTR LVREGRNMTF HCGQKILHKK GKVYWYKDQE PLEFSYPGYL
360 370 380 390
ALGEAQLSII ANAVNEGTYT CVVRRHQRVL STYSWRVRVR N
Length:391
Mass (Da):44,451
Last modified:November 1, 1998 - v1
Checksum:i8C9675020F02F632
GO
Isoform 2 (identifier: O88676-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-157: Missing.

Show »
Length:377
Mass (Da):42,946
Checksum:i8C30623283790A72
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021561144 – 157Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085742 mRNA. Translation: AAC34886.1.
AK146510 mRNA. Translation: BAE27223.1.
BC107357 mRNA. Translation: AAI07358.1.
BC107358 mRNA. Translation: AAI07359.1.
CCDSiCCDS19034.1. [O88676-1]
RefSeqiNP_001307164.1. NM_001320235.1. [O88676-2]
NP_036115.1. NM_011985.3. [O88676-1]
XP_006538945.1. XM_006538882.3. [O88676-1]
UniGeneiMm.29373.

Genome annotation databases

EnsembliENSMUST00000030937; ENSMUSP00000030937; ENSMUSG00000029061. [O88676-1]
GeneIDi26561.
KEGGimmu:26561.
UCSCiuc008wed.1. mouse. [O88676-1]
uc012dqs.1. mouse. [O88676-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMMP23_MOUSE
AccessioniPrimary (citable) accession number: O88676
Secondary accession number(s): Q3KNC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 1, 1998
Last modified: August 30, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families