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Protein

Matrix metalloproteinase-23

Gene

Mmp23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by TIMP2.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei79 – 802Cleavage; by furin-like proteaseSequence Analysis
Metal bindingi212 – 2121Zinc; catalyticPROSITE-ProRule annotation
Active sitei213 – 2131PROSITE-ProRule annotation
Metal bindingi216 – 2161Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.022.
TCDBi8.B.14.2.1. the sea anemone peptide toxin, class 1 (bgk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-23 (EC:3.4.24.-)
Short name:
MMP-23
Alternative name(s):
CAMP metalloproteinase
Short name:
CA-MMP
Cleaved into the following chain:
Gene namesi
Name:Mmp23
Synonyms:Cammp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1347361. Mmp23.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity
  • Membrane 1 Publication; Single-pass type II membrane protein 1 Publication

  • Note: A secreted form produced by proteolytic cleavage may also exist.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST
Topological domaini40 – 391352LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Matrix metalloproteinase-23PRO_0000259915Add
BLAST
Propeptidei1 – 7979Sequence AnalysisPRO_0000259916Add
BLAST
Chaini80 – 391312Matrix metalloproteinase-23, soluble formPRO_0000259917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi256 ↔ 290By similarity
Disulfide bondi263 ↔ 283By similarity
Disulfide bondi272 ↔ 287By similarity
Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi322 ↔ 371By similarity

Post-translational modificationi

N-glycosylated.By similarity
Proteolytic cleavage might yield an active form.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiO88676.

PTM databases

PhosphoSiteiO88676.

Expressioni

Tissue specificityi

Expressed at relatively high level in heart, lung and spleen. Not detected in brain, liver, skeletal muscle, kidney and testis.1 Publication

Developmental stagei

Expressed at relatively high level at 7 days old embryo compared to those at stadges day 11, 15 and 17.1 Publication

Gene expression databases

BgeeiO88676.
CleanExiMM_MMP23.
GenevisibleiO88676. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030937.

Structurei

3D structure databases

ProteinModelPortaliO88676.
SMRiO88676. Positions 88-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini256 – 29035ShKTPROSITE-ProRule annotationAdd
BLAST
Domaini298 – 38386Ig-like C2-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 794Poly-Arg

Domaini

The ShKT domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4 (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG245056.
GeneTreeiENSGT00760000119132.
HOGENOMiHOG000231157.
HOVERGENiHBG053177.
InParanoidiO88676.
KOiK08001.
OMAiAIMHLNA.
OrthoDBiEOG7KSX8P.
PhylomeDBiO88676.
TreeFamiTF315428.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR024079. MetalloPept_cat_dom.
IPR028687. MMP23.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10201:SF7. PTHR10201:SF7. 1 hit.
PfamiPF00413. Peptidase_M10. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00409. IG. 1 hit.
SM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88676-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCRACLRPE ASGAVQGRWL GAALSGLCLL SALALLEWLG APTETAWRAA
60 70 80 90 100
QGNVDAPNVG SSTAQVPRLL TMSVTRRRRY TLTPARLRWD HFNLTYRVLS
110 120 130 140 150
FPRNLLSPEE TRRGLAAAFR MWSDVSPFSF REVAPERPSD LKIGFYPVNH
160 170 180 190 200
TDCLVSAVHH CFDGPTGELA HAFFPPHGGI HFDDSEYWVL GPTRYSWKKG
210 220 230 240 250
VWLTNLVHVA AHEIGHALGL MHSQQDQALM HLNATLRGWK ALSQDELWGL
260 270 280 290 300
HRLYGCLDRI FVCASWARKG FCDVRQRLMK RLCPRSCDFC YEFPFPTVAT
310 320 330 340 350
TTSPTRTKTR LVREGRNMTF HCGQKILHKK GKVYWYKDQE PLEFSYPGYL
360 370 380 390
ALGEAQLSII ANAVNEGTYT CVVRRHQRVL STYSWRVRVR N
Length:391
Mass (Da):44,451
Last modified:November 1, 1998 - v1
Checksum:i8C9675020F02F632
GO
Isoform 2 (identifier: O88676-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-157: Missing.

Show »
Length:377
Mass (Da):42,946
Checksum:i8C30623283790A72
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei144 – 15714Missing in isoform 2. 1 PublicationVSP_021561Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085742 mRNA. Translation: AAC34886.1.
AK146510 mRNA. Translation: BAE27223.1.
BC107357 mRNA. Translation: AAI07358.1.
BC107358 mRNA. Translation: AAI07359.1.
CCDSiCCDS19034.1. [O88676-1]
RefSeqiNP_036115.1. NM_011985.2. [O88676-1]
XP_006538945.1. XM_006538882.2. [O88676-1]
XP_006538946.1. XM_006538883.2. [O88676-2]
UniGeneiMm.29373.

Genome annotation databases

EnsembliENSMUST00000030937; ENSMUSP00000030937; ENSMUSG00000029061. [O88676-1]
GeneIDi26561.
KEGGimmu:26561.
UCSCiuc008wed.1. mouse. [O88676-1]
uc012dqs.1. mouse. [O88676-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085742 mRNA. Translation: AAC34886.1.
AK146510 mRNA. Translation: BAE27223.1.
BC107357 mRNA. Translation: AAI07358.1.
BC107358 mRNA. Translation: AAI07359.1.
CCDSiCCDS19034.1. [O88676-1]
RefSeqiNP_036115.1. NM_011985.2. [O88676-1]
XP_006538945.1. XM_006538882.2. [O88676-1]
XP_006538946.1. XM_006538883.2. [O88676-2]
UniGeneiMm.29373.

3D structure databases

ProteinModelPortaliO88676.
SMRiO88676. Positions 88-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030937.

Protein family/group databases

MEROPSiM10.022.
TCDBi8.B.14.2.1. the sea anemone peptide toxin, class 1 (bgk) family.

PTM databases

PhosphoSiteiO88676.

Proteomic databases

PRIDEiO88676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030937; ENSMUSP00000030937; ENSMUSG00000029061. [O88676-1]
GeneIDi26561.
KEGGimmu:26561.
UCSCiuc008wed.1. mouse. [O88676-1]
uc012dqs.1. mouse. [O88676-2]

Organism-specific databases

CTDi26561.
MGIiMGI:1347361. Mmp23.

Phylogenomic databases

eggNOGiNOG245056.
GeneTreeiENSGT00760000119132.
HOGENOMiHOG000231157.
HOVERGENiHBG053177.
InParanoidiO88676.
KOiK08001.
OMAiAIMHLNA.
OrthoDBiEOG7KSX8P.
PhylomeDBiO88676.
TreeFamiTF315428.

Miscellaneous databases

NextBioi304627.
PROiO88676.
SOURCEiSearch...

Gene expression databases

BgeeiO88676.
CleanExiMM_MMP23.
GenevisibleiO88676. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR024079. MetalloPept_cat_dom.
IPR028687. MMP23.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10201:SF7. PTHR10201:SF7. 1 hit.
PfamiPF00413. Peptidase_M10. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00409. IG. 1 hit.
SM00254. ShKT. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CA-MMP: a matrix metalloproteinase with a novel cysteine array, but without the classic cysteine switch."
    Pei D.
    FEBS Lett. 457:262-270(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Stomach.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiMMP23_MOUSE
AccessioniPrimary (citable) accession number: O88676
Secondary accession number(s): Q3KNC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.