Reviewed, UniProtKB/Swiss-Prot O88673 (DGKA_MOUSE)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase alpha
      Short name=DAG kinase alpha
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase alpha
      Short name=DGK-alpha
    80 kDa diacylglycerol kinase

Gene names

Name:	Dgka
Synonyms:	Dagk1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: MGI plasma membrane Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from direct assay. Source: MGI phospholipid binding Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Diacylglycerol kinase alpha		PRO_0000218454
Regions
	Domain	111 – 146	36	EF-hand 1
	Domain	156 – 191	36	EF-hand 2
	Domain	368 – 501	134	DAGKc
	Calcium binding	124 – 135	12	1 Probable
	Calcium binding	169 – 180	12	2 Probable
	Zinc finger	206 – 254	49	Phorbol-ester/DAG-type 1
	Zinc finger	270 – 320	51	Phorbol-ester/DAG-type 2
Amino acid modifications
	Modified residue	22	1	Phosphotyrosine By similarity
	Modified residue	479	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	317	1	G → V in AAC33483. Ref.1
	Sequence conflict	392	1	K → N in AAC33483. Ref.1
	Sequence conflict	398	1	N → D in AAC33483. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O88673-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: A7808E4F8FD83350	FASTA	730	82,762
	10 20 30 40 50 60 MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY LGFEQFMKMY 70 80 90 100 110 120 LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV YCYFTLLEGG RPEDKLEFTF 130 140 150 160 170 180 KLYDMDRNGI LDSTEVEKII LQMMRVAEYL DWDVSELRPI LQEMMREMDQ DGSGSVSLDE 190 200 210 220 230 240 WVRAGATTVP LLVLLGMDVT MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK 250 260 270 280 290 300 YIVHDHCAMK AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH 310 320 330 340 350 360 CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT TDDTSLCTPE 370 380 390 400 410 420 AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR QVFDLKDGPE PGLRFFKDVP 430 440 450 460 470 480 QFRILVCGGD GTVGWVLETI DKANFATVPP VAVLPLGTGN DLARCLRWGR GYEGENLRKI 490 500 510 520 530 540 LKDIELSKVV YLDRWFLEVI PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP 550 560 570 580 590 600 EKFNSRMKNK LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST 610 620 630 640 650 660 HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE VVGIEGAIEM 670 680 690 700 710 720 GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA PCTIKITHKN QMPMLMGPPS 730 NSYNFFGFWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sanjuan M.A., Carrera A.C., Merida I. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF085219 mRNA. Translation: AAC33483.1. BC006713 mRNA. Translation: AAH06713.1.
IPI	IPI00315730.
RefSeq	NP_058091.2.
UniGene	Mm.291235
3D structure databases
HSSP	HSSP built from PDB template 1M39 based on UniProtKB P41208.
SMR	O88673. Positions 1-119.
ModBase	Search...
Protein-protein interaction databases
STRING	O88673.
PTM databases
PhosphoSite	O88673.
Proteomic databases
PRIDE	O88673.
Genome annotation databases
Ensembl	ENSMUST00000026414; ENSMUSP00000026414; ENSMUSG00000025357; Mus musculus. [Genome view]
GeneID	13139.
KEGG	mmu:13139.
UCSC	uc007hoc.1. mouse.
Organism-specific databases
CTD	13139.
MGI	MGI:102952. Dgka.
Phylogenomic databases
HOGENOM	O88673.
HOVERGEN	O88673.
OMA	LWGDTKR.
Enzyme and pathway databases
BRENDA	2.7.1.107. 244.
Gene expression databases
ArrayExpress	O88673.
Bgee	O88673.
CleanEx	MM_DGKA.
Genevestigator	O88673.
GermOnline	ENSMUSG00000025357. Mus musculus.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283214.
SOURCE	Search...

Entry information

Entry name

DGKA_MOUSE

Accession

Primary (citable) accession number: O88673
Secondary accession number(s): Q922X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	March 27, 2002
Last modified:	November 3, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)