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O88673

- DGKA_MOUSE

UniProt

O88673 - DGKA_MOUSE

Protein

Diacylglycerol kinase alpha

Gene

Dgka

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi124 – 135121CuratedAdd
    BLAST
    Calcium bindingi169 – 180122CuratedAdd
    BLAST
    Zinc fingeri206 – 25449Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri270 – 32051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: MGI
    4. NAD+ kinase activity Source: InterPro
    5. phospholipid binding Source: MGI

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase alpha (EC:2.7.1.107)
    Short name:
    DAG kinase alpha
    Alternative name(s):
    80 kDa diacylglycerol kinase
    Diglyceride kinase alpha
    Short name:
    DGK-alpha
    Gene namesi
    Name:Dgka
    Synonyms:Dagk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:102952. Dgka.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. plasma membrane Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Diacylglycerol kinase alphaPRO_0000218454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei479 – 4791N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO88673.
    PaxDbiO88673.
    PRIDEiO88673.

    PTM databases

    PhosphoSiteiO88673.

    Expressioni

    Gene expression databases

    BgeeiO88673.
    CleanExiMM_DGKA.
    GenevestigatoriO88673.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026414.

    Structurei

    3D structure databases

    ProteinModelPortaliO88673.
    SMRiO88673. Positions 1-199, 381-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini111 – 14636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini156 – 19136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini368 – 501134DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri206 – 25449Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri270 – 32051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    InParanoidiO88673.
    KOiK00901.
    OMAiLVGLHCV.
    OrthoDBiEOG75XGK8.
    PhylomeDBiO88673.
    TreeFamiTF313104.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88673-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY    50
    LGFEQFMKMY LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV 100
    YCYFTLLEGG RPEDKLEFTF KLYDMDRNGI LDSTEVEKII LQMMRVAEYL 150
    DWDVSELRPI LQEMMREMDQ DGSGSVSLDE WVRAGATTVP LLVLLGMDVT 200
    MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK YIVHDHCAMK 250
    AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH 300
    CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT 350
    TDDTSLCTPE AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR 400
    QVFDLKDGPE PGLRFFKDVP QFRILVCGGD GTVGWVLETI DKANFATVPP 450
    VAVLPLGTGN DLARCLRWGR GYEGENLRKI LKDIELSKVV YLDRWFLEVI 500
    PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP EKFNSRMKNK 550
    LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST 600
    HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE 650
    VVGIEGAIEM GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA 700
    PCTIKITHKN QMPMLMGPPS NSYNFFGFWS 730
    Length:730
    Mass (Da):82,762
    Last modified:March 27, 2002 - v2
    Checksum:iA7808E4F8FD83350
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti317 – 3171G → V in AAC33483. 1 PublicationCurated
    Sequence conflicti392 – 3921K → N in AAC33483. 1 PublicationCurated
    Sequence conflicti398 – 3981N → D in AAC33483. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF085219 mRNA. Translation: AAC33483.1.
    BC006713 mRNA. Translation: AAH06713.1.
    CCDSiCCDS24290.1.
    RefSeqiNP_058091.2. NM_016811.2.
    UniGeneiMm.291235.

    Genome annotation databases

    EnsembliENSMUST00000026414; ENSMUSP00000026414; ENSMUSG00000025357.
    GeneIDi13139.
    KEGGimmu:13139.
    UCSCiuc007hoc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF085219 mRNA. Translation: AAC33483.1 .
    BC006713 mRNA. Translation: AAH06713.1 .
    CCDSi CCDS24290.1.
    RefSeqi NP_058091.2. NM_016811.2.
    UniGenei Mm.291235.

    3D structure databases

    ProteinModelPortali O88673.
    SMRi O88673. Positions 1-199, 381-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026414.

    PTM databases

    PhosphoSitei O88673.

    Proteomic databases

    MaxQBi O88673.
    PaxDbi O88673.
    PRIDEi O88673.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026414 ; ENSMUSP00000026414 ; ENSMUSG00000025357 .
    GeneIDi 13139.
    KEGGi mmu:13139.
    UCSCi uc007hoc.1. mouse.

    Organism-specific databases

    CTDi 1606.
    MGIi MGI:102952. Dgka.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    InParanoidi O88673.
    KOi K00901.
    OMAi LVGLHCV.
    OrthoDBi EOG75XGK8.
    PhylomeDBi O88673.
    TreeFami TF313104.

    Enzyme and pathway databases

    Reactomei REACT_219232. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    ChiTaRSi DGKA. mouse.
    NextBioi 283214.
    PROi O88673.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88673.
    CleanExi MM_DGKA.
    Genevestigatori O88673.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sanjuan M.A., Carrera A.C., Merida I.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiDGKA_MOUSE
    AccessioniPrimary (citable) accession number: O88673
    Secondary accession number(s): Q922X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3