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O88673

- DGKA_MOUSE

UniProt

O88673 - DGKA_MOUSE

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Protein

Diacylglycerol kinase alpha

Gene

Dgka

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi124 – 135121CuratedAdd
BLAST
Calcium bindingi169 – 180122CuratedAdd
BLAST
Zinc fingeri206 – 25449Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 32051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: MGI
  4. NAD+ kinase activity Source: InterPro
  5. phospholipid binding Source: MGI

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:Dgka
Synonyms:Dagk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:102952. Dgka.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. plasma membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Diacylglycerol kinase alphaPRO_0000218454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei479 – 4791N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO88673.
PaxDbiO88673.
PRIDEiO88673.

PTM databases

PhosphoSiteiO88673.

Expressioni

Gene expression databases

BgeeiO88673.
CleanExiMM_DGKA.
GenevestigatoriO88673.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026414.

Structurei

3D structure databases

ProteinModelPortaliO88673.
SMRiO88673. Positions 1-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 14636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini156 – 19136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini368 – 501134DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri206 – 25449Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 32051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiO88673.
KOiK00901.
OMAiLVGLHCV.
OrthoDBiEOG75XGK8.
PhylomeDBiO88673.
TreeFamiTF313104.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88673-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY
60 70 80 90 100
LGFEQFMKMY LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV
110 120 130 140 150
YCYFTLLEGG RPEDKLEFTF KLYDMDRNGI LDSTEVEKII LQMMRVAEYL
160 170 180 190 200
DWDVSELRPI LQEMMREMDQ DGSGSVSLDE WVRAGATTVP LLVLLGMDVT
210 220 230 240 250
MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK YIVHDHCAMK
260 270 280 290 300
AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH
310 320 330 340 350
CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT
360 370 380 390 400
TDDTSLCTPE AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR
410 420 430 440 450
QVFDLKDGPE PGLRFFKDVP QFRILVCGGD GTVGWVLETI DKANFATVPP
460 470 480 490 500
VAVLPLGTGN DLARCLRWGR GYEGENLRKI LKDIELSKVV YLDRWFLEVI
510 520 530 540 550
PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP EKFNSRMKNK
560 570 580 590 600
LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST
610 620 630 640 650
HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE
660 670 680 690 700
VVGIEGAIEM GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA
710 720 730
PCTIKITHKN QMPMLMGPPS NSYNFFGFWS
Length:730
Mass (Da):82,762
Last modified:March 27, 2002 - v2
Checksum:iA7808E4F8FD83350
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti317 – 3171G → V in AAC33483. 1 PublicationCurated
Sequence conflicti392 – 3921K → N in AAC33483. 1 PublicationCurated
Sequence conflicti398 – 3981N → D in AAC33483. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085219 mRNA. Translation: AAC33483.1.
BC006713 mRNA. Translation: AAH06713.1.
CCDSiCCDS24290.1.
RefSeqiNP_058091.2. NM_016811.2.
UniGeneiMm.291235.

Genome annotation databases

EnsembliENSMUST00000026414; ENSMUSP00000026414; ENSMUSG00000025357.
GeneIDi13139.
KEGGimmu:13139.
UCSCiuc007hoc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085219 mRNA. Translation: AAC33483.1 .
BC006713 mRNA. Translation: AAH06713.1 .
CCDSi CCDS24290.1.
RefSeqi NP_058091.2. NM_016811.2.
UniGenei Mm.291235.

3D structure databases

ProteinModelPortali O88673.
SMRi O88673. Positions 1-199.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000026414.

PTM databases

PhosphoSitei O88673.

Proteomic databases

MaxQBi O88673.
PaxDbi O88673.
PRIDEi O88673.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026414 ; ENSMUSP00000026414 ; ENSMUSG00000025357 .
GeneIDi 13139.
KEGGi mmu:13139.
UCSCi uc007hoc.1. mouse.

Organism-specific databases

CTDi 1606.
MGIi MGI:102952. Dgka.

Phylogenomic databases

eggNOGi NOG47311.
GeneTreei ENSGT00760000119050.
HOGENOMi HOG000252931.
HOVERGENi HBG051345.
InParanoidi O88673.
KOi K00901.
OMAi LVGLHCV.
OrthoDBi EOG75XGK8.
PhylomeDBi O88673.
TreeFami TF313104.

Enzyme and pathway databases

Reactomei REACT_219232. Effects of PIP2 hydrolysis.

Miscellaneous databases

ChiTaRSi DGKA. mouse.
NextBioi 283214.
PROi O88673.
SOURCEi Search...

Gene expression databases

Bgeei O88673.
CleanExi MM_DGKA.
Genevestigatori O88673.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Sanjuan M.A., Carrera A.C., Merida I.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiDGKA_MOUSE
AccessioniPrimary (citable) accession number: O88673
Secondary accession number(s): Q922X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 27, 2002
Last modified: October 29, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3