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Protein

GTP-binding protein RAD

Gene

Rrad

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 105GTPBy similarity8
Nucleotide bindingi203 – 206GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RAD
Gene namesi
Name:Rrad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1930943. Rrad.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show increased susceptibility to thoracic transverse aortic constriction (TAC)-induced cardiac hypertrophy. TAC-induced CAMKII phosphorylation in the heart is also significantly increased.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105S → A: Loss of interaction with CACNB2. QT prolongation and ventricular arrhythmias, caused by the augmentation of L-type Ca(2+) channels in the heart. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224791 – 308GTP-binding protein RADAdd BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Omega-N-methylarginineCombined sources1
Modified residuei26PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiO88667.
MaxQBiO88667.
PaxDbiO88667.
PRIDEiO88667.

PTM databases

iPTMnetiO88667.
PhosphoSitePlusiO88667.

Expressioni

Gene expression databases

CleanExiMM_RRAD.

Interactioni

Subunit structurei

Interacts with Calmodulin preferentially in the inactive, GDP-bound form. Interacts with CAMK2D (By similarity). Interacts with CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane.By similarity1 Publication

Protein-protein interaction databases

IntActiO88667. 1 interactor.
MINTiMINT-101873.
STRINGi10090.ENSMUSP00000034351.

Structurei

3D structure databases

ProteinModelPortaliO88667.
SMRiO88667.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 297Calmodulin-bindingBy similarityAdd BLAST20

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiO88667.
PhylomeDBiO88667.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNGGSGAS GSRGAGGRER DRRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LAPGSLATTA AGTRTQGQRL DWPEGSSDSL SSGGSGSEEG VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGIEDGPEA EAAGHTYDRS ITVDGEEASL LVYDIWEEDG
160 170 180 190 200
GCWLPGHCMA MGDAYVIVYS ITDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKED NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK

SCHDLSVL
Length:308
Mass (Da):33,279
Last modified:November 1, 1998 - v1
Checksum:i698F0A3BCE605D53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084466 mRNA. Translation: AAC33133.1.
UniGeneiMm.29467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084466 mRNA. Translation: AAC33133.1.
UniGeneiMm.29467.

3D structure databases

ProteinModelPortaliO88667.
SMRiO88667.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88667. 1 interactor.
MINTiMINT-101873.
STRINGi10090.ENSMUSP00000034351.

PTM databases

iPTMnetiO88667.
PhosphoSitePlusiO88667.

Proteomic databases

EPDiO88667.
MaxQBiO88667.
PaxDbiO88667.
PRIDEiO88667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1930943. Rrad.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000246961.
HOVERGENiHBG104899.
InParanoidiO88667.
PhylomeDBiO88667.

Miscellaneous databases

PROiO88667.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RRAD.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028869. RRAD.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF74. PTHR24070:SF74. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF038017. GTP-binding_GEM. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD_MOUSE
AccessioniPrimary (citable) accession number: O88667
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.