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O88665

- BRD7_MOUSE

UniProt

O88665 - BRD7_MOUSE

Protein

Bromodomain-containing protein 7

Gene

Brd7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase By similarity. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR.By similarity3 Publications

    GO - Molecular functioni

    1. lysine-acetylated histone binding Source: UniProtKB
    2. p53 binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. transcription coactivator activity Source: UniProtKB
    5. transcription corepressor activity Source: UniProtKB
    6. transcription factor binding Source: MGI
    7. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. negative regulation of cell proliferation Source: UniProtKB
    3. negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
    4. negative regulation of transcription, DNA-templated Source: UniProtKB
    5. positive regulation of histone acetylation Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. regulation of transcription from RNA polymerase II promoter Source: Ensembl
    8. transcription, DNA-templated Source: UniProtKB-KW
    9. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bromodomain-containing protein 7
    Alternative name(s):
    75 kDa bromodomain protein
    Gene namesi
    Name:Brd7
    Synonyms:Bp75
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1349766. Brd7.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 651651Bromodomain-containing protein 7PRO_0000227665Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei279 – 2791PhosphoserineBy similarity
    Modified residuei328 – 3281N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiO88665.
    PRIDEiO88665.

    PTM databases

    PhosphoSiteiO88665.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Developmental stagei

    Expressed ubiquitously from 10.5 to 18.5 dpc.1 Publication

    Gene expression databases

    ArrayExpressiO88665.
    BgeeiO88665.
    CleanExiMM_BRD7.
    GenevestigatoriO88665.

    Interactioni

    Subunit structurei

    Interacts with IRF2 and HNRPUL1 By similarity. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at 'Lys-14' (H3K14ac). Has low affinity for histone H3 acetylated at 'Lys-9' (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at 'Lys-9' (H3K9ac) and at 'Lys-14' (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at 'Lys-8' (H3K8ac) (in vitro) By similarity. Interacts with TRIM24, PTPN13 and DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Pik3r1P2645011EBI-643930,EBI-641764
    Pik3r2O089084EBI-643930,EBI-643570
    Ptpn13Q645123EBI-643930,EBI-4284057

    Protein-protein interaction databases

    BioGridi205094. 4 interactions.
    IntActiO88665. 4 interactions.
    MINTiMINT-1704124.

    Structurei

    3D structure databases

    ProteinModelPortaliO88665.
    SMRiO88665. Positions 129-238, 338-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 21871BromoPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili536 – 56732Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi65 – 9632Nuclear localization signalBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi57 – 9135Lys-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bromo domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Bromodomain, Coiled coil

    Phylogenomic databases

    eggNOGiCOG5076.
    GeneTreeiENSGT00530000063939.
    HOGENOMiHOG000070022.
    HOVERGENiHBG071934.
    InParanoidiO88665.
    KOiK11723.
    OMAiGDIVSTY.
    OrthoDBiEOG7D2FDM.
    PhylomeDBiO88665.
    TreeFamiTF106439.

    Family and domain databases

    Gene3Di1.20.920.10. 1 hit.
    InterProiIPR001487. Bromodomain.
    IPR021900. DUF3512.
    [Graphical view]
    PfamiPF00439. Bromodomain. 1 hit.
    PF12024. DUF3512. 1 hit.
    [Graphical view]
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00297. BROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF47370. SSF47370. 1 hit.
    PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88665-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKKHKKHKS DRHFYEEYVE KPLKLVLKVG GSEVTELSTG SSGHDSSLFE    50
    DRSDHDKHKD RKRKKRKKGE KQAPGEEKGR KRRRVKEDKK KRDRDRAENE 100
    VDRDLQCHVP IRLDLPPEKP LTSSLAKQEE VEQTPLQEAL NQLMRQLQRK 150
    DPSAFFSFPV TDFIAPGYSM IIKHPMDFST MKEKIKNNDY QSIEELKDNF 200
    KLMCTNAMIY NKPETIYYKA AKKLLHSGMK ILSQERIQSL KQSIDFMSDL 250
    QKTRKQKERT DACQSGEDSG CWQREREDSG DAETQAFRSP AKDNKRKDKD 300
    VLEDKWRSSN SEREHEQIER VVQESGGKLT RRLANSQCEF ERRKPDGTTT 350
    LGLLHPVDPI VGEPGYCPVR LGMTTGRLQS GVNTLQGFKE DKRNRVTPVL 400
    YLNYGPYSSY APHYDSTFAN ISKDDSDLIY STYGEDSDLP NNFSISEFLA 450
    TCQDYPYVMA DSLLDVLTKG GHSRSLQDLD MSSPEDEGQT RALDTAKEAE 500
    ITQIEPTGRL ESSSQDRLTA LQAVTTFGAP AEVFDSEEAE VFQRKLDETT 550
    RLLRELQEAQ NERLSTRPPP NMICLLGPSY REMYLAEQVT NNLKELTQQV 600
    TPGDVVSIHG VRKAMGISVP SPIVGNSFVD LTGECEEPKE TSTAECGPDA 650
    S 651
    Length:651
    Mass (Da):74,000
    Last modified:November 1, 1998 - v1
    Checksum:i5D34B4F14FD51350
    GO

    Sequence cautioni

    The sequence BAE23823.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021D → G in BAE23823. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084259 mRNA. Translation: AAC33302.1.
    AK004429 mRNA. Translation: BAB23299.1.
    AK138934 mRNA. Translation: BAE23823.1. Different initiation.
    AK142758 mRNA. Translation: BAE25187.1.
    CCDSiCCDS22510.1.
    RefSeqiNP_036177.1. NM_012047.2.
    UniGeneiMm.5400.

    Genome annotation databases

    EnsembliENSMUST00000034085; ENSMUSP00000034085; ENSMUSG00000031660.
    GeneIDi26992.
    KEGGimmu:26992.
    UCSCiuc009mrn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084259 mRNA. Translation: AAC33302.1 .
    AK004429 mRNA. Translation: BAB23299.1 .
    AK138934 mRNA. Translation: BAE23823.1 . Different initiation.
    AK142758 mRNA. Translation: BAE25187.1 .
    CCDSi CCDS22510.1.
    RefSeqi NP_036177.1. NM_012047.2.
    UniGenei Mm.5400.

    3D structure databases

    ProteinModelPortali O88665.
    SMRi O88665. Positions 129-238, 338-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205094. 4 interactions.
    IntActi O88665. 4 interactions.
    MINTi MINT-1704124.

    PTM databases

    PhosphoSitei O88665.

    Proteomic databases

    PaxDbi O88665.
    PRIDEi O88665.

    Protocols and materials databases

    DNASUi 26992.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034085 ; ENSMUSP00000034085 ; ENSMUSG00000031660 .
    GeneIDi 26992.
    KEGGi mmu:26992.
    UCSCi uc009mrn.2. mouse.

    Organism-specific databases

    CTDi 29117.
    MGIi MGI:1349766. Brd7.

    Phylogenomic databases

    eggNOGi COG5076.
    GeneTreei ENSGT00530000063939.
    HOGENOMi HOG000070022.
    HOVERGENi HBG071934.
    InParanoidi O88665.
    KOi K11723.
    OMAi GDIVSTY.
    OrthoDBi EOG7D2FDM.
    PhylomeDBi O88665.
    TreeFami TF106439.

    Miscellaneous databases

    NextBioi 304935.
    PROi O88665.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88665.
    Bgeei O88665.
    CleanExi MM_BRD7.
    Genevestigatori O88665.

    Family and domain databases

    Gene3Di 1.20.920.10. 1 hit.
    InterProi IPR001487. Bromodomain.
    IPR021900. DUF3512.
    [Graphical view ]
    Pfami PF00439. Bromodomain. 1 hit.
    PF12024. DUF3512. 1 hit.
    [Graphical view ]
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00297. BROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47370. SSF47370. 1 hit.
    PROSITEi PS50014. BROMODOMAIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and molecular characterization of BP75, a novel bromodomain-containing protein."
      Cuppen E., van Ham M., Pepers B., Wieringa B., Hendriks W.
      FEBS Lett. 459:291-298(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PTPN13, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-293 AND 478-651.
      Strain: C57BL/6J.
      Tissue: Aorta, Embryo, Fetal head and Vein.
    3. "BP75, bromodomain-containing M(r) 75,000 protein, binds dishevelled-1 and enhances Wnt signaling by inactivating glycogen synthase kinase-3 beta."
      Kim S., Lee J., Park J., Chung J.
      Cancer Res. 63:4792-4795(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH DVL1.
    4. "BRD7, a novel PBAF-specific SWI/SNF subunit, is required for target gene activation and repression in embryonic stem cells."
      Kaeser M.D., Aslanian A., Dong M.Q., Yates J.R. III, Emerson B.M.
      J. Biol. Chem. 283:32254-32263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH SMARCA4/BRG1; SMARCE1/BAF57; DPF2/BAF45D; SMARCC1/BAF155 AND ARID2.
    5. "TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells."
      Kikuchi M., Okumura F., Tsukiyama T., Watanabe M., Miyajima N., Tanaka J., Imamura M., Hatakeyama S.
      Biochim. Biophys. Acta 1793:1828-1836(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRIM24, FUNCTION, SUBCELLULAR LOCATION.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiBRD7_MOUSE
    AccessioniPrimary (citable) accession number: O88665
    Secondary accession number(s): Q3UQ56, Q3UU06, Q9CT78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3