O88665 (BRD7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bromodomain-containing protein 7

Alternative name(s):
75 kDa bromodomain protein

Gene names

Name:	Brd7
Synonyms:	Bp75

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase By similarity. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR. Ref.3 Ref.4 Ref.6
Subunit structure	Interacts with IRF2 and HNRPUL1 By similarity. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at 'Lys-14' (H3K14ac). Has low affinity for histone H3 acetylated at 'Lys-9' (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at 'Lys-9' (H3K9ac) and at 'Lys-14' (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at 'Lys-8' (H3K8ac) (in vitro) By similarity. Interacts with TRIM24, PTPN13 and DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex. Ref.1 Ref.3 Ref.6
Subcellular location	Nucleus Ref.1 Ref.6.
Tissue specificity	Ubiquitous. Ref.1
Developmental stage	Expressed ubiquitously from 10.5 to 18.5 dpc. Ref.1
Sequence similarities	Contains 1 bromo domain.
Sequence caution	The sequence BAE23823.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Disease	Tumor suppressor
Domain	Bromodomain Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of G1/S transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of histone acetylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from physical interaction Ref.1. Source: MGI
Molecular function	histone acetyl-lysine binding Inferred from sequence or structural similarity. Source: UniProtKB p53 binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB transcription corepressor activity Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pik3r1	P26450	11	EBI-643930,EBI-641764
Pik3r2	O08908	4	EBI-643930,EBI-643570

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Bromodomain-containing protein 7		PRO_0000227665
Regions
	Domain	148 – 218	71	Bromo
	Coiled coil	536 – 567	32	Potential
	Motif	65 – 96	32	Nuclear localization signal By similarity
	Compositional bias	57 – 91	35	Lys-rich
Amino acid modifications
	Modified residue	279	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	102	1	D → G in BAE23823. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O88665 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 5D34B4F14FD51350	FASTA	651	74,000
	10 20 30 40 50 60 MGKKHKKHKS DRHFYEEYVE KPLKLVLKVG GSEVTELSTG SSGHDSSLFE DRSDHDKHKD 70 80 90 100 110 120 RKRKKRKKGE KQAPGEEKGR KRRRVKEDKK KRDRDRAENE VDRDLQCHVP IRLDLPPEKP 130 140 150 160 170 180 LTSSLAKQEE VEQTPLQEAL NQLMRQLQRK DPSAFFSFPV TDFIAPGYSM IIKHPMDFST 190 200 210 220 230 240 MKEKIKNNDY QSIEELKDNF KLMCTNAMIY NKPETIYYKA AKKLLHSGMK ILSQERIQSL 250 260 270 280 290 300 KQSIDFMSDL QKTRKQKERT DACQSGEDSG CWQREREDSG DAETQAFRSP AKDNKRKDKD 310 320 330 340 350 360 VLEDKWRSSN SEREHEQIER VVQESGGKLT RRLANSQCEF ERRKPDGTTT LGLLHPVDPI 370 380 390 400 410 420 VGEPGYCPVR LGMTTGRLQS GVNTLQGFKE DKRNRVTPVL YLNYGPYSSY APHYDSTFAN 430 440 450 460 470 480 ISKDDSDLIY STYGEDSDLP NNFSISEFLA TCQDYPYVMA DSLLDVLTKG GHSRSLQDLD 490 500 510 520 530 540 MSSPEDEGQT RALDTAKEAE ITQIEPTGRL ESSSQDRLTA LQAVTTFGAP AEVFDSEEAE 550 560 570 580 590 600 VFQRKLDETT RLLRELQEAQ NERLSTRPPP NMICLLGPSY REMYLAEQVT NNLKELTQQV 610 620 630 640 650 TPGDVVSIHG VRKAMGISVP SPIVGNSFVD LTGECEEPKE TSTAECGPDA S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of BP75, a novel bromodomain-containing protein." Cuppen E., van Ham M., Pepers B., Wieringa B., Hendriks W. FEBS Lett. 459:291-298(1999) [PubMed: 10526152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PTPN13, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-293 AND 478-651. Strain: C57BL/6J. Tissue: Aorta, Embryo, Fetal head and Vein.
[3]	"BP75, bromodomain-containing M(r) 75,000 protein, binds dishevelled-1 and enhances Wnt signaling by inactivating glycogen synthase kinase-3 beta." Kim S., Lee J., Park J., Chung J. Cancer Res. 63:4792-4795(2003) [PubMed: 12941796] [Abstract] Cited for: FUNCTION, INTERACTION WITH DVL1.
[4]	"BRD7, a novel PBAF-specific SWI/SNF subunit, is required for target gene activation and repression in embryonic stem cells." Kaeser M.D., Aslanian A., Dong M.Q., Yates J.R. III, Emerson B.M. J. Biol. Chem. 283:32254-32263(2008) [PubMed: 18809673] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH SMARCA4/BRG1; SMARCE1/BAF57; DPF2/BAF45D; SMARCC1/BAF155 AND ARID2.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-279, MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells." Kikuchi M., Okumura F., Tsukiyama T., Watanabe M., Miyajima N., Tanaka J., Imamura M., Hatakeyama S. Biochim. Biophys. Acta 1793:1828-1836(2009) [PubMed: 19909775] [Abstract] Cited for: INTERACTION WITH TRIM24, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF084259 mRNA. Translation: AAC33302.1. AK004429 mRNA. Translation: BAB23299.1. AK138934 mRNA. Translation: BAE23823.1. Different initiation. AK142758 mRNA. Translation: BAE25187.1.
IPI	IPI00133099.
RefSeq	NP_036177.1. NM_012047.2.
UniGene	Mm.5400.
3D structure databases
ProteinModelPortal	O88665.
SMR	O88665. Positions 129-238.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88665. 2 interactions.
STRING	O88665.
PTM databases
PhosphoSite	O88665.
Proteomic databases
PRIDE	O88665.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034085; ENSMUSP00000034085; ENSMUSG00000031660.
GeneID	26992.
KEGG	mmu:26992.
UCSC	uc009mrn.2. mouse.
Organism-specific databases
CTD	29117.
MGI	MGI:1349766. Brd7.
Phylogenomic databases
eggNOG	roNOG09335.
HOGENOM	HBG444928.
HOVERGEN	HBG071934.
InParanoid	O88665.
OMA	EDKFKSN.
OrthoDB	EOG4D26PG.
PhylomeDB	O88665.
Gene expression databases
ArrayExpress	O88665.
Bgee	O88665.
CleanEx	MM_BRD7.
Genevestigator	O88665.
GermOnline	ENSMUSG00000031660. Mus musculus.
Family and domain databases
InterPro	IPR001487. Bromodomain. IPR021900. DUF3512. [Graphical view]
Gene3D	G3DSA:1.20.920.10. Bromodomain. 1 hit.
KO	K11723.
Pfam	PF00439. Bromodomain. 1 hit. PF12024. DUF3512. 1 hit. [Graphical view]
PRINTS	PR00503. BROMODOMAIN.
SMART	SM00297. BROMO. 1 hit. [Graphical view]
SUPFAM	SSF47370. Bromodomain. 1 hit.
PROSITE	PS00633. BROMODOMAIN_1. False negative. PS50014. BROMODOMAIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304935.
SOURCE	Search...

Entry information

Entry name

BRD7_MOUSE

Accession

Primary (citable) accession number: O88665
Secondary accession number(s): Q3UQ56, Q3UU06, Q9CT78

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program