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Protein

Epithelial membrane protein 2

Gene

Emp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:17609206, PubMed:14978215). Facilitates surface trafficking and the formation of lipid rafts bearing GPI-anchor proteins (PubMed:14978215). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (PubMed:12763482). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:12189152). Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (By similarity). Regulates cell migration and cell contraction through PTK2 and SRC activation (By similarity). Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (By similarity). Positively regulates cell proliferation (By similarity). Plays a role during cell death and cell blebbing (By similarity). Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (By similarity). Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956, PubMed:16216233). May play a role in glomerular filtration (By similarity).By similarity6 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • kinase binding Source: MGI
  • protein kinase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial membrane protein 2
Short name:
EMP-2
Alternative name(s):
Protein XMP
Gene namesi
Name:Emp2
Synonyms:Xmp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1098726. Emp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence analysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence analysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: UniProtKB
  • apical plasma membrane Source: MGI
  • cell surface Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172Epithelial membrane protein 2PRO_0000164659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence analysis
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO88662.
PRIDEiO88662.

Expressioni

Tissue specificityi

Expressed in both the endometrial epithelial and stromal cells during the embryo implantation period (PubMed:16487956). Expressed in the corneal epithelium, the sclera, the nerve fiber layer of the inner retina and retinal ganglion cell layer, the nonpigmented epithelium of the ciliary body and the optic nerve (PubMed:12710941).2 Publications

Gene expression databases

BgeeiO88662.
CleanExiMM_EMP2.
ExpressionAtlasiO88662. baseline and differential.
GenevisibleiO88662. MM.

Interactioni

Subunit structurei

Interacts with PTK2; regulates PTK2 activation and localization (By similarity). Interacts with ITGB3; regulates the levels of the heterodimer ITGA5-ITGB3 integrin surface expression (By similarity). Interacts with P2RX7 (via C-terminus) (By similarity). Interacts with ITGB1; the interaction may be direct or indirect and ITGB1 has a heterodimer form (PubMed:12189152).By similarity1 Publication

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • kinase binding Source: MGI
  • protein kinase binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077466.

Structurei

3D structure databases

ProteinModelPortaliO88662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH06. Eukaryota.
ENOG4111QXD. LUCA.
HOGENOMiHOG000059542.
HOVERGENiHBG001690.
InParanoidiO88662.
OMAiIQLMSCL.
OrthoDBiEOG7NKKMT.
PhylomeDBiO88662.
TreeFamiTF330414.

Family and domain databases

InterProiIPR003933. EMP-2.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PANTHERiPTHR10671:SF32. PTHR10671:SF32. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01455. EPMEMPROT2.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVILAFIIV FHIVSTALLF ISTIDNAWWV GDSFSADLWR VCTNSTNCTE
60 70 80 90 100
INELTGPEAF EGYSVMQAVQ ATMILSTILS CISFLIFLLQ LFRLKQGERF
110 120 130 140 150
VLTSIIQLMS CLCVMIGASI YTDRRQDLHQ QNRKLYYLLQ EGSYGYSFIL
160 170
AWVAFAFTFI SGLMYMILRK RK
Length:172
Mass (Da):19,747
Last modified:November 1, 1998 - v1
Checksum:i6110C073BC75FB6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083876 mRNA. Translation: AAC33108.1.
CCDSiCCDS37250.1.
RefSeqiNP_031955.2. NM_007929.2.
UniGeneiMm.246009.

Genome annotation databases

EnsembliENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505.
GeneIDi13731.
KEGGimmu:13731.
UCSCiuc007ydj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083876 mRNA. Translation: AAC33108.1.
CCDSiCCDS37250.1.
RefSeqiNP_031955.2. NM_007929.2.
UniGeneiMm.246009.

3D structure databases

ProteinModelPortaliO88662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077466.

Proteomic databases

PaxDbiO88662.
PRIDEiO88662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505.
GeneIDi13731.
KEGGimmu:13731.
UCSCiuc007ydj.1. mouse.

Organism-specific databases

CTDi2013.
MGIiMGI:1098726. Emp2.

Phylogenomic databases

eggNOGiENOG410IH06. Eukaryota.
ENOG4111QXD. LUCA.
HOGENOMiHOG000059542.
HOVERGENiHBG001690.
InParanoidiO88662.
OMAiIQLMSCL.
OrthoDBiEOG7NKKMT.
PhylomeDBiO88662.
TreeFamiTF330414.

Miscellaneous databases

NextBioi284530.
PROiO88662.
SOURCEiSearch...

Gene expression databases

BgeeiO88662.
CleanExiMM_EMP2.
ExpressionAtlasiO88662. baseline and differential.
GenevisibleiO88662. MM.

Family and domain databases

InterProiIPR003933. EMP-2.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PANTHERiPTHR10671:SF32. PTHR10671:SF32. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01455. EPMEMPROT2.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chromosomal mapping of Tmp (Emp1), Xmp (Emp2), and Ymp (Emp3), genes encoding membrane proteins related to Pmp22."
    Ben-Porath I., Kozak C.A., Benvenisty N.
    Genomics 49:443-447(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The tetraspan protein epithelial membrane protein-2 interacts with beta1 integrins and regulates adhesion."
    Wadehra M., Iyer R., Goodglick L., Braun J.
    J. Biol. Chem. 277:41094-41100(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ITGB1, SUBCELLULAR LOCATION.
  3. "The tetraspan protein EMP2 increases surface expression of class I major histocompatibility complex proteins and susceptibility to CTL-mediated cell death."
    Wadehra M., Su H., Gordon L.K., Goodglick L., Braun J.
    Clin. Immunol. 107:129-136(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Epithelial membrane protein-2 is expressed in discrete anatomical regions of the eye."
    Wadehra M., Sulur G.G., Braun J., Gordon L.K., Goodglick L.
    Exp. Mol. Pathol. 74:106-112(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "The tetraspan protein EMP2 modulates the surface expression of caveolins and glycosylphosphatidyl inositol-linked proteins."
    Wadehra M., Goodglick L., Braun J.
    Mol. Biol. Cell 15:2073-2083(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium."
    Wadehra M., Forbes A., Pushkarna N., Goodglick L., Gordon L.K., Williams C.J., Braun J.
    Dev. Biol. 287:336-345(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Knockdown of the tetraspan protein epithelial membrane protein-2 inhibits implantation in the mouse."
    Wadehra M., Dayal M., Mainigi M., Ord T., Iyer R., Braun J., Williams C.J.
    Dev. Biol. 292:430-441(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  8. Cited for: FUNCTION.

Entry informationi

Entry nameiEMP2_MOUSE
AccessioniPrimary (citable) accession number: O88662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.