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Protein

Ethanolamine-phosphate cytidylyltransferase

Gene

Pcyt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine (By similarity).By similarity

Catalytic activityi

CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline kinase alpha (Chka), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei277 – 2771CTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2402CTPBy similarity
Nucleotide bindingi247 – 2504CTPBy similarity
Nucleotide bindingi325 – 3284CTPBy similarity
Nucleotide bindingi354 – 3585CTPBy similarity

GO - Molecular functioni

  • ethanolamine-phosphate cytidylyltransferase activity Source: RGD

GO - Biological processi

  • phosphatidylethanolamine biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00742.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine-phosphate cytidylyltransferase (EC:2.7.7.14)
Alternative name(s):
CTP:phosphoethanolamine cytidylyltransferase
Phosphorylethanolamine transferase
Gene namesi
Name:Pcyt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi619970. Pcyt2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Ethanolamine-phosphate cytidylyltransferasePRO_0000208463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561PhosphoserineBy similarity
Modified residuei360 – 3601PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88637.
PRIDEiO88637.

PTM databases

iPTMnetiO88637.

Expressioni

Gene expression databases

GenevisibleiO88637. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051835.

Structurei

3D structure databases

ProteinModelPortaliO88637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG2803. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00550000075065.
HOGENOMiHOG000187618.
HOVERGENiHBG000865.
InParanoidiO88637.
KOiK00967.
OMAiSEYREYT.
OrthoDBiEOG7CRTPS.
PhylomeDBiO88637.
TreeFamiTF106337.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88637-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIRNGHGAGG AAGLKGPGGQ RTVRVWCDGC YDMVHYGHSN QLRQARAMGD
60 70 80 90 100
YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE
110 120 130 140 150
TLDKHNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV
160 170 180 190 200
GRMLLVTKAH HSSQEMSSEY REYADSFGKP PHPTPAGDTL SSEVSSQCPG
210 220 230 240 250
GQSPWTGVSQ FLQTSQKIIQ FASGKEPQPG ETVIYVAGAF DLFHIGHVDF
260 270 280 290 300
LQEVHKLAKR PYVIAGLHFD QEVNRYKGKN YPIMNLHERT LSVLACRYVS
310 320 330 340 350
EVVIGAPYSV TAELLNHFKV DLVCHGKTEI VPDRDGSDPY EEPKRRGIFC
360 370 380 390 400
QIDSGSDLTT DLIVQRIIKN RLEYEARNQK KEAKELAFLE ALRQQEAQPR

GETD
Length:404
Mass (Da):45,220
Last modified:November 1, 1998 - v1
Checksum:i4B0B6A9315B32CE3
GO
Isoform 2 (identifier: O88637-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-197: Missing.

Note: No experimental confirmation available.
Show »
Length:386
Mass (Da):43,431
Checksum:i0A28624314BE329E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei180 – 19718Missing in isoform 2. 1 PublicationVSP_022116Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080568 mRNA. Translation: AAC28864.1.
BC078772 mRNA. Translation: AAH78772.1.
RefSeqiNP_446020.1. NM_053568.1. [O88637-1]
XP_006247997.1. XM_006247935.2. [O88637-2]
UniGeneiRn.7291.

Genome annotation databases

EnsembliENSRNOT00000054952; ENSRNOP00000051835; ENSRNOG00000036684. [O88637-1]
GeneIDi89841.
KEGGirno:89841.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080568 mRNA. Translation: AAC28864.1.
BC078772 mRNA. Translation: AAH78772.1.
RefSeqiNP_446020.1. NM_053568.1. [O88637-1]
XP_006247997.1. XM_006247935.2. [O88637-2]
UniGeneiRn.7291.

3D structure databases

ProteinModelPortaliO88637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051835.

PTM databases

iPTMnetiO88637.

Proteomic databases

PaxDbiO88637.
PRIDEiO88637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054952; ENSRNOP00000051835; ENSRNOG00000036684. [O88637-1]
GeneIDi89841.
KEGGirno:89841.

Organism-specific databases

CTDi5833.
RGDi619970. Pcyt2.

Phylogenomic databases

eggNOGiKOG2803. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00550000075065.
HOGENOMiHOG000187618.
HOVERGENiHBG000865.
InParanoidiO88637.
KOiK00967.
OMAiSEYREYT.
OrthoDBiEOG7CRTPS.
PhylomeDBiO88637.
TreeFamiTF106337.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00742.
ReactomeiR-RNO-1483213. Synthesis of PE.

Miscellaneous databases

PROiO88637.

Gene expression databases

GenevisibleiO88637. RN.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of CTP:phosphoethanolamine cytidylyltransferase cDNA from rat liver."
    Bladergroen B.A., Houweling M., Geelen M.J.H., van Golde L.M.G.
    Biochem. J. 343:107-114(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Wistar.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 298-319, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCY2_RAT
AccessioniPrimary (citable) accession number: O88637
Secondary accession number(s): Q6AZ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.