ID   SEM3F_MOUSE             Reviewed;         785 AA.
AC   O88632; E9QNC5; O88633;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   24-JAN-2024, entry version 183.
DE   RecName: Full=Semaphorin-3F;
DE   AltName: Full=Semaphorin IV;
DE            Short=Sema IV;
DE   Flags: Precursor;
GN   Name=Sema3f;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
RX   PubMed=9875739; DOI=10.1097/00001756-199812010-00038;
RA   Eckhardt F., Meyerhans A.;
RT   "Cloning and expression pattern of a murine semaphorin homologous to H-sema
RT   IV.";
RL   NeuroReport 9:3975-3979(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B;
CC         IsoId=O88632-1; Sequence=Displayed;
CC       Name=A;
CC         IsoId=O88632-2; Sequence=VSP_006042;
CC   -!- TISSUE SPECIFICITY: Expressed ubiquitously in adulthood. During
CC       embryogenesis, expressed in subregions of the central nervous system
CC       and various other tissues like skin, kidney, lung and intestine.
CC   -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
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DR   EMBL; AF080090; AAC28108.1; -; mRNA.
DR   EMBL; AF080091; AAC28109.1; -; mRNA.
DR   EMBL; AC152718; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC162905; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC010976; AAH10976.1; -; mRNA.
DR   CCDS; CCDS23505.1; -. [O88632-2]
DR   CCDS; CCDS81069.1; -. [O88632-1]
DR   RefSeq; NP_001298080.1; NM_001311151.1. [O88632-1]
DR   RefSeq; NP_035479.2; NM_011349.3. [O88632-2]
DR   RefSeq; XP_006511739.1; XM_006511676.3.
DR   RefSeq; XP_017168717.1; XM_017313228.1.
DR   AlphaFoldDB; O88632; -.
DR   SMR; O88632; -.
DR   BioGRID; 203165; 4.
DR   STRING; 10090.ENSMUSP00000141865; -.
DR   GlyCosmos; O88632; 2 sites, No reported glycans.
DR   GlyGen; O88632; 2 sites.
DR   PhosphoSitePlus; O88632; -.
DR   MaxQB; O88632; -.
DR   PaxDb; 10090-ENSMUSP00000079400; -.
DR   ProteomicsDB; 256613; -. [O88632-1]
DR   ProteomicsDB; 256614; -. [O88632-2]
DR   Pumba; O88632; -.
DR   Antibodypedia; 30785; 267 antibodies from 29 providers.
DR   DNASU; 20350; -.
DR   Ensembl; ENSMUST00000080560.9; ENSMUSP00000079400.4; ENSMUSG00000034684.13. [O88632-2]
DR   Ensembl; ENSMUST00000192727.6; ENSMUSP00000141865.2; ENSMUSG00000034684.13. [O88632-1]
DR   GeneID; 20350; -.
DR   KEGG; mmu:20350; -.
DR   UCSC; uc009rms.1; mouse. [O88632-1]
DR   UCSC; uc009rmt.1; mouse. [O88632-2]
DR   AGR; MGI:1096347; -.
DR   CTD; 6405; -.
DR   MGI; MGI:1096347; Sema3f.
DR   VEuPathDB; HostDB:ENSMUSG00000034684; -.
DR   eggNOG; KOG3611; Eukaryota.
DR   GeneTree; ENSGT00940000156703; -.
DR   HOGENOM; CLU_009051_5_0_1; -.
DR   InParanoid; O88632; -.
DR   OMA; HQDYIFY; -.
DR   OrthoDB; 5342713at2759; -.
DR   TreeFam; TF352628; -.
DR   BioGRID-ORCS; 20350; 5 hits in 80 CRISPR screens.
DR   PRO; PR:O88632; -.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; O88632; Protein.
DR   Bgee; ENSMUSG00000034684; Expressed in ectoplacental cone and 177 other cell types or tissues.
DR   ExpressionAtlas; O88632; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; HDA:BHF-UCL.
DR   GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR   GO; GO:0045499; F:chemorepellent activity; IDA:MGI.
DR   GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR   GO; GO:0048846; P:axon extension involved in axon guidance; IDA:MGI.
DR   GO; GO:0007411; P:axon guidance; IDA:MGI.
DR   GO; GO:0021785; P:branchiomotor neuron axon guidance; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0021612; P:facial nerve structural organization; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0050919; P:negative chemotaxis; IDA:MGI.
DR   GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IDA:MGI.
DR   GO; GO:0021675; P:nerve development; IMP:BHF-UCL.
DR   GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR   GO; GO:1901166; P:neural crest cell migration involved in autonomic nervous system development; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR   GO; GO:0099175; P:regulation of postsynapse organization; IDA:SynGO.
DR   GO; GO:0071526; P:semaphorin-plexin signaling pathway; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0061549; P:sympathetic ganglion development; IMP:BHF-UCL.
DR   GO; GO:0097490; P:sympathetic neuron projection extension; IMP:BHF-UCL.
DR   GO; GO:0097491; P:sympathetic neuron projection guidance; IMP:BHF-UCL.
DR   GO; GO:0021637; P:trigeminal nerve structural organization; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0036484; P:trunk neural crest cell migration; IMP:MGI.
DR   GO; GO:0036486; P:ventral trunk neural crest cell migration; IMP:ParkinsonsUK-UCL.
DR   CDD; cd05871; Ig_Sema3; 1.
DR   CDD; cd11254; Sema_3F; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 3.30.1680.10; ligand-binding face of the semaphorins, domain 2; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR003598; Ig_sub2.
DR   InterPro; IPR016201; PSI.
DR   InterPro; IPR001627; Semap_dom.
DR   InterPro; IPR036352; Semap_dom_sf.
DR   InterPro; IPR027231; Semaphorin.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR11036; SEMAPHORIN; 1.
DR   PANTHER; PTHR11036:SF27; SEMAPHORIN-3F; 1.
DR   Pfam; PF01403; Sema; 1.
DR   SMART; SM00409; IG; 1.
DR   SMART; SM00408; IGc2; 1.
DR   SMART; SM00423; PSI; 1.
DR   SMART; SM00630; Sema; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   SUPFAM; SSF103575; Plexin repeat; 1.
DR   SUPFAM; SSF101912; Sema domain; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS51004; SEMA; 1.
DR   Genevisible; O88632; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..785
FT                   /note="Semaphorin-3F"
FT                   /id="PRO_0000032321"
FT   DOMAIN          31..545
FT                   /note="Sema"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00352"
FT   DOMAIN          605..695
FT                   /note="Ig-like C2-type"
FT   REGION          583..602
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          753..785
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        769..785
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        53
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        126
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        104..115
FT                   /evidence="ECO:0000250"
FT   DISULFID        133..142
FT                   /evidence="ECO:0000250"
FT   DISULFID        300..412
FT                   /evidence="ECO:0000250"
FT   DISULFID        324..372
FT                   /evidence="ECO:0000250"
FT   DISULFID        548..566
FT                   /evidence="ECO:0000250"
FT   DISULFID        678..746
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         153..183
FT                   /note="Missing (in isoform A)"
FT                   /evidence="ECO:0000303|PubMed:15489334,
FT                   ECO:0000303|PubMed:9875739"
FT                   /id="VSP_006042"
FT   CONFLICT        350
FT                   /note="V -> I (in Ref. 1; AAC28108/AAC28109 and 3;
FT                   AAH10976)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        756
FT                   /note="R -> S (in Ref. 1; AAC28108/AAC28109 and 3;
FT                   AAH10976)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   785 AA;  88549 MW;  8F3F0935096C24A1 CRC64;
     MLVTAFILWA SLLTGAWPAT PIQDQLPATP RVRLSFKELK ATGTAHFFNF LLNTTDYRIL
     LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR IEECILSGKD GNGECGNFVR
     LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR AQALPWTQMQ VVRGRGSRAT DGADRPTPTA
     PRQDYIFYLE PEKLESGKGK CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ
     TAMRTDQYNS RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQNP AVYARIGRIC
     LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV RNPVIYAVFT
     SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS
     MKSTKDYPDE VINFMRTHPL MYQAVYPLQR RPLVVRTGAP YRLTTVAVDQ VDAADGRYEV
     LFLGTDRGTV QKVIVLPKDD QEVEELMLEE VEVFKEPAPV KTMTISSKRQ QLYVASAVGV
     THLSLHRCQA YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG
     FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPSDRRREI RAEDRFLRTE
     QGLLLRALQL GDRGLYSCTA TENNFKHIVT RVQLHVLGRD AVHAALFPPL AVSVPPPPGT
     GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW RHVPPRPREA PGALRPPELQ DQKKPRNRRH
     HPPDT
//