Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O88627 (S28A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/nucleoside cotransporter 2
Alternative name(s):
Concentrative nucleoside transporter 2
Short name=CNT 2
Na(+)/nucleoside cotransporter 2
Sodium-coupled nucleoside transporter 2
Sodium/purine nucleoside cotransporter
Short name=SPNT
Solute carrier family 28 member 2
Gene names
Name:Slc28a2
Synonyms:Cnt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sodium-dependent and purine-selective. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine) By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 660660Sodium/nucleoside cotransporter 2
PRO_0000070451

Regions

Transmembrane82 – 10221Helical; Potential
Transmembrane106 – 12520Helical; Potential
Transmembrane150 – 16819Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane202 – 22221Helical; Potential
Transmembrane235 – 25521Helical; Potential
Transmembrane262 – 28221Helical; Potential
Transmembrane297 – 31620Helical; Potential
Transmembrane338 – 35720Helical; Potential
Transmembrane364 – 38320Helical; Potential
Transmembrane425 – 44521Helical; Potential
Transmembrane456 – 47621Helical; Potential
Transmembrane531 – 55121Helical; Potential
Transmembrane569 – 58921Helical; Potential

Experimental info

Sequence conflict21E → K in AAC28858. Ref.1
Sequence conflict141T → R in AAC28858. Ref.1
Sequence conflict181C → G in AAC28858. Ref.1
Sequence conflict801R → K in AAC28858. Ref.1
Sequence conflict921A → T in AAC28858. Ref.1
Sequence conflict1541V → I in AAC28858. Ref.1
Sequence conflict3371A → T in AAC28858. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O88627 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 64522D068A2138EC

FASTA66072,916
        10         20         30         40         50         60 
MEKSKGRKSV SQATVENCME NPGLELMEGG NLEQRYTQEE VTQGHSLEDG LGHSSLWSRR 

        70         80         90        100        110        120 
IFQPFTKARS FFERHAGLFR KILLGLLCLA YAAYFLAACI LNFQRALALF VITCLVIFIL 

       130        140        150        160        170        180 
ACHFLKKFFP KEQLRCLKPL ENTHLNLWAK RVFVGLSVVG LILWLALDTA QRPEQLISFA 

       190        200        210        220        230        240 
GICMFILILF ACSKHHSAVC WRTVFWGLGL QFIFGILVIR TEPGFNAFQW LGDQIQIFLA 

       250        260        270        280        290        300 
YTVEGSSFVF GDTLVQNVFA FQSLPIIIFF GCVMSILYYL GLVQWVIQKV AWFLQITMGT 

       310        320        330        340        350        360 
TAAETLAVAG NIFVGMTEAP LLIRPYLADM TISEIHAVMT GGFATIAGTV LGAFISFGID 

       370        380        390        400        410        420 
ASSLISASVM AAPCALALSK LVYPEVEESK FKSKEGLKLP RGEERNILEA ASNGATDAIS 

       430        440        450        460        470        480 
LVANVAANLI AFLAVLAFIN ATLSWLGEMV DIHGLSFQVI CSYVLRPMVF MMGVQWADCP 

       490        500        510        520        530        540 
LVAEIVGVKF FINEFVAYQQ LSQYKNKRLS GVEEWINGEK QWISVKAEII TTFSLCGFAN 

       550        560        570        580        590        600 
LSSIGITLGG LTSMIPQRKS DLCKIVVRAL FTGACVSFIS ACMAGILYVP RGAETDCVSF 

       610        620        630        640        650        660 
LNTNFTNRTY ETYVCCRELF QSTSLNGTNM PSFSGPWQDN VSSLRNLASC CDLYTSTVCA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, genomic organization and chromosomal localization of the gene encoding the murine sodium-dependent, purine-selective, concentrative nucleoside transporter (CNT2)."
Patel D.H., Crawford C.R., Naeve C.W., Belt J.A.
Gene 242:51-58(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Adipose tissue and Bone marrow.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF079853 mRNA. Translation: AAC28858.1.
AK080957 mRNA. Translation: BAC38094.1.
AK152776 mRNA. Translation: BAE31488.1.
AL844566 Genomic DNA. Translation: CAM16323.1.
RefSeqNP_766568.1. NM_172980.2.
XP_006499663.1. XM_006499600.1.
XP_006499664.1. XM_006499601.1.
UniGeneMm.29510.

3D structure databases

ProteinModelPortalO88627.
SMRO88627. Positions 176-587.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996278.

Chemistry

ChEMBLCHEMBL1287613.

PTM databases

PhosphoSiteO88627.

Proteomic databases

PaxDbO88627.
PRIDEO88627.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028652; ENSMUSP00000028652; ENSMUSG00000027219.
ENSMUST00000110524; ENSMUSP00000106153; ENSMUSG00000027219.
ENSMUST00000110525; ENSMUSP00000106154; ENSMUSG00000027219.
GeneID269346.
KEGGmmu:269346.
UCSCuc008mas.1. mouse.

Organism-specific databases

CTD9153.
MGIMGI:1913105. Slc28a2.

Phylogenomic databases

eggNOGCOG1972.
GeneTreeENSGT00390000016025.
HOGENOMHOG000267658.
HOVERGENHBG054073.
InParanoidQ8BJP1.
KOK11536.
OMAYNNTVCA.
OrthoDBEOG7RV9FR.
TreeFamTF314131.

Gene expression databases

BgeeO88627.
GenevestigatorO88627.

Family and domain databases

InterProIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. Nucleos_tra2_C.
IPR011642. Nucleoside_recog_Gate.
IPR002668. Nuclsd_transpt2.
[Graphical view]
PANTHERPTHR10590. PTHR10590. 1 hit.
PfamPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00804. nupC. 1 hit.
ProtoNetSearch...

Other

NextBio392797.
PROO88627.
SOURCESearch...

Entry information

Entry nameS28A2_MOUSE
AccessionPrimary (citable) accession number: O88627
Secondary accession number(s): Q8BJP1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot