Reviewed, UniProtKB/Swiss-Prot O88622 (PARG_MOUSE)

Names and origin

Protein names

Recommended name:
    Poly(ADP-ribose) glycohydrolase
    EC=3.2.1.143

Gene names

Name:

Parg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	969 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.
Catalytic activity	Hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the poly(ADP-ribose) glycohydrolase family.
Sequence caution	The sequence BC050892 differs from that shown. Reason: Erroneous termination at position 962. Translated as Met. The sequence BC059827 differs from that shown. Reason: Erroneous termination at position 962. Translated as Met.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro detection of bacterium Inferred from mutant phenotype. Source: MGI response to DNA damage stimulus Inferred from mutant phenotype. Source: MGI
Cellular component	cytosol Inferred from mutant phenotype. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	poly(ADP-ribose) glycohydrolase activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O88622-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O88622-2)

The sequence of this isoform differs from the canonical sequence as follows:
     920-920: K → E
     921-969: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 969	969	Poly(ADP-ribose) glycohydrolase		PRO_0000066603
Regions
	Motif	10 – 16	7	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	135	1	Phosphoserine Ref.4
	Modified residue	137	1	Phosphothreonine By similarity
	Modified residue	195	1	Phosphoserine By similarity
	Modified residue	197	1	Phosphothreonine By similarity
	Modified residue	256	1	Phosphoserine Ref.4
	Modified residue	259	1	Phosphoserine Ref.4
	Modified residue	311	1	Phosphoserine By similarity
	Modified residue	496	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	920	1	K → E in isoform 2.		VSP_011771
	Alternative sequence	921 – 969	49	Missing in isoform 2.		VSP_011772
Experimental info
	Sequence conflict	13 – 14	2	RP → A in AAC28735. Ref.1
	Sequence conflict	305	1	K → R in AAC28735. Ref.1
	Sequence conflict	370	1	A → V in BC050892. Ref.3
	Sequence conflict	370	1	A → V in BC059827. Ref.3
	Sequence conflict	872	1	A → V in BAC29519. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: 2626996A53AC48ED	FASTA	969	109,324
	10 20 30 40 50 60 MSAGPGWEPC TKRPRWGAAG TSAPTASDSR SFPGRQRRVL DPKDAPVQFR VPPSSPACVS 70 80 90 100 110 120 GRAGPHRGNA TSFVFKQKTI TTWMDTKGPK TAESESKENN NTRIDSMMSS VQKDNFYPHK 130 140 150 160 170 180 VEKLENVPQL NLDKSPTEKS SQYLNQQQTA SVCKWQNEGK HAEQLLASEP PAGTPLPKQL 190 200 210 220 230 240 SNANIGQSPH TDDHSDTDHE EDRDNQQFLT PIKLANTKPT VGDGQARSNC KCSGSRQSVK 250 260 270 280 290 300 DCTGCQQEEV DVLPESPLSD VGAEDIGTGP KNDNKLTGQE SSLGDSPPFE KESEPESPMD 310 320 330 340 350 360 VDNSKNSCQD SEADEETSPV FDEQDDRSSQ TANKLSSCQA READGDLRKR YLTKGSEVRL 370 380 390 400 410 420 HFQFEGENNA GTSDLNAKPS GNSSSLNVEC RSSKQHGKRD SKITDHFMRI SKSEDRRKEQ 430 440 450 460 470 480 CEVRHQRTER KIPKYIPPNL PPEKKWLGTP IEEMRKMPRC GIHLPSLRPS ASHTVTVRVD 490 500 510 520 530 540 LLRAGEVPKP FPTHYKDLWD NKHVKMPCSE QNLYPVEDEN GERTAGSRWE LIQTALLNKF 550 560 570 580 590 600 TRPQNLKDAI LKYNVAYSKK WDFTALVDFW DKVLEEAEAQ HLYQSILPDM VKIALCLPNI 610 620 630 640 650 660 CTQPIPLLKQ KMNHSVTMSQ EQIASLLANA FFCTFPRRNA KMKSEYSSYP DINFNRLFEG 670 680 690 700 710 720 RSSRKPEKLK TLFCYFRRVT EKKPTGLVTF TRQSLEDFPE WERCEKPLTR LHVTYEGTIE 730 740 750 760 770 780 GNGRGMLQVD FANRFVGGGV TGAGLVQEEI RFLINPELIV SRLFTEVLDH NECLIITGTE 790 800 810 820 830 840 QYSEYTGYAE TYRWARSHED GSEKDDWQRR CTEIVAIDAL HFRRYLDQFV PEKVRRELNK 850 860 870 880 890 900 AYCGFLRPGV PSENLSAVAT GNWGCGAFGG DARLKALIQI LAAAAAERDV VYFTFGDSEL 910 920 930 940 950 960 MRDIYSMHTF LTERKLDVGK VYKLLLRYYN EECRNCSTPG PDIKLYPFIY HAVESSAETT DMPGQKAGT
	Isoform 2. Checksum: 2EA3C15C421B09A1	FASTA	920	103,771
	10 20 30 40 50 60 MSAGPGWEPC TKRPRWGAAG TSAPTASDSR SFPGRQRRVL DPKDAPVQFR VPPSSPACVS 70 80 90 100 110 120 GRAGPHRGNA TSFVFKQKTI TTWMDTKGPK TAESESKENN NTRIDSMMSS VQKDNFYPHK 130 140 150 160 170 180 VEKLENVPQL NLDKSPTEKS SQYLNQQQTA SVCKWQNEGK HAEQLLASEP PAGTPLPKQL 190 200 210 220 230 240 SNANIGQSPH TDDHSDTDHE EDRDNQQFLT PIKLANTKPT VGDGQARSNC KCSGSRQSVK 250 260 270 280 290 300 DCTGCQQEEV DVLPESPLSD VGAEDIGTGP KNDNKLTGQE SSLGDSPPFE KESEPESPMD 310 320 330 340 350 360 VDNSKNSCQD SEADEETSPV FDEQDDRSSQ TANKLSSCQA READGDLRKR YLTKGSEVRL 370 380 390 400 410 420 HFQFEGENNA GTSDLNAKPS GNSSSLNVEC RSSKQHGKRD SKITDHFMRI SKSEDRRKEQ 430 440 450 460 470 480 CEVRHQRTER KIPKYIPPNL PPEKKWLGTP IEEMRKMPRC GIHLPSLRPS ASHTVTVRVD 490 500 510 520 530 540 LLRAGEVPKP FPTHYKDLWD NKHVKMPCSE QNLYPVEDEN GERTAGSRWE LIQTALLNKF 550 560 570 580 590 600 TRPQNLKDAI LKYNVAYSKK WDFTALVDFW DKVLEEAEAQ HLYQSILPDM VKIALCLPNI 610 620 630 640 650 660 CTQPIPLLKQ KMNHSVTMSQ EQIASLLANA FFCTFPRRNA KMKSEYSSYP DINFNRLFEG 670 680 690 700 710 720 RSSRKPEKLK TLFCYFRRVT EKKPTGLVTF TRQSLEDFPE WERCEKPLTR LHVTYEGTIE 730 740 750 760 770 780 GNGRGMLQVD FANRFVGGGV TGAGLVQEEI RFLINPELIV SRLFTEVLDH NECLIITGTE 790 800 810 820 830 840 QYSEYTGYAE TYRWARSHED GSEKDDWQRR CTEIVAIDAL HFRRYLDQFV PEKVRRELNK 850 860 870 880 890 900 AYCGFLRPGV PSENLSAVAT GNWGCGAFGG DARLKALIQI LAAAAAERDV VYFTFGDSEL 910 920 MRDIYSMHTF LTERKLDVGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assignment of the poly(ADP-ribose) glycohydrolase gene (PARG) to human chromosome 10q11.23 and mouse chromosome 14B by in situ hybridization." Ame J.-C., Apiou F., Jacobson E.L., Jacobson M.K. Cytogenet. Cell Genet. 85:269-270(1999) [PubMed: 10449915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Bone.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135; SER-256 AND SER-259, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF079557 mRNA. Translation: AAC28735.1. AK036656 mRNA. Translation: BAC29519.1. BC050892 mRNA. No translation available. BC059827 mRNA. No translation available.
IPI	IPI00471055. IPI00471056.
UniGene	Mm.15962
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O88622.
PTM databases
PhosphoSite	O88622.
Proteomic databases
PRIDE	O88622.
Genome annotation databases
Ensembl	ENSMUST00000022470; ENSMUSP00000022470; ENSMUSG00000021911; Mus musculus. [Genome view]
UCSC	uc007syq.1. mouse.
Organism-specific databases
MGI	MGI:1347094. Parg.
Phylogenomic databases
HOGENOM	O88622.
HOVERGEN	O88622.
Enzyme and pathway databases
BRENDA	3.2.1.143. 244.
Gene expression databases
ArrayExpress	O88622.
Bgee	O88622.
CleanEx	MM_PARG.
Genevestigator	O88622.
GermOnline	ENSMUSG00000021911. Mus musculus.
Family and domain databases
InterPro	IPR007724. Poly_GlycHdrlase. [Graphical view]
PANTHER	PTHR12837. Poly_glchydro. 1 hit.
Pfam	PF05028. PARG_cat. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PARG_MOUSE

Accession

Primary (citable) accession number: O88622
Secondary accession number(s): Q80YQ6, Q8CB72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	October 13, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)