Voltage-dependent calcium channel gamma-2 subunit

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O88602 (CCG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Voltage-dependent calcium channel gamma-2 subunit

Alternative name(s):
Neuronal voltage-gated calcium channel gamma-2 subunit
Stargazin
Transmembrane AMPAR regulatory protein gamma-2
Short name=TARP gamma-2

Gene names

Name:	Cacng2
Synonyms:	Stg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state By similarity.
Subunit structure	The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with GRIA1 By similarity. Ref.2 Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Brain.
Post-translational modification	Phosphorylation of Thr-321 by PKA impairs interaction with DLG1 and DLG4.
Involvement in disease	Note=Defects in Cacng2 cause the stargazer (stg) phenotype. Stg mice have spike-wave seizures characteristic of absence epilepsy, with accompanying defects in the cerebellum and inner ear.
Sequence similarities	Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ionic channel Voltage-gated channel
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	membrane depolarization Inferred from mutant phenotype. Source: MGI membrane hyperpolarization Inferred from mutant phenotype. Source: MGI neuromuscular junction development Inferred from mutant phenotype. Source: MGI regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Inferred from sequence or structural similarity. Source: UniProtKB transmission of nerve impulse Inferred from genetic interaction. Source: MGI
Cellular component	alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	voltage-gated calcium channel activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Voltage-dependent calcium channel gamma-2 subunit

PRO_0000164674

Regions

Transmembrane

10 – 30

Helical; Potential

Transmembrane

104 – 124

Helical; Potential

Transmembrane

134 – 154

Helical; Potential

Transmembrane

182 – 202

Helical; Potential

Amino acid modifications

Modified residue

253

Phosphoserine Ref.4

Modified residue

271

Phosphotyrosine Ref.5

Modified residue

321

Phosphothreonine; by PKA Ref.2

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

321

T → A: Abolishes phosphorylation. Ref.2

Mutagenesis

321

T → D or E: No interaction with DLG1 and DLG4. Ref.2

Mutagenesis

323

V → A: No interaction with DLG1 and DLG4. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O88602 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: AA9D475606A0FBA4
Show »

FASTA

323

35,895

        10         20         30         40         50         60 
MGLFDRGVQM LLTTVGAFAA FSLMTIAVGT DYWLYSRGVC KTKSVSENET SKKNEEVMTH 

        70         80         90        100        110        120 
SGLWRTCCLE GNFKGLCKQI DHFPEDADYE ADTAEYFLRA VRASSIFPIL SVILLFMGGL 

       130        140        150        160        170        180 
CIAASEFYKT RHNIILSAGI FFVSAGLSNI IGIIVYISAN AGDPSKSDSK KNSYSYGWSF 

       190        200        210        220        230        240 
YFGALSFIIA EMVGVLAVHM FIDRHKQLRA TARATDYLQA SAITRIPSYR YRYQRRSRSS 

       250        260        270        280        290        300 
SRSTEPSHSR DASPVGVKGF NTLPSTEISM YTLSRDPLKA ATTPTATYNS DRDNSFLQVH 

       310        320 
NCIQKDSKDS LHANTANRRT TPV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit." Letts V.A., Felix R., Biddlecome G.H., Arikkath J., Mahaffey C.L., Valenzuela A., Bartlett F.S. II, Mori Y., Campbell K.P., Frankel W.N. Nat. Genet. 19:340-347(1998) [PubMed: 9697694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Brain.
[2]	"Phosphorylation of stargazin by protein kinase A regulates its interaction with PSD-95." Choi J., Ko J., Park E., Lee J.-R., Yoon J., Lim S., Kim E. J. Biol. Chem. 277:12359-12363(2002) [PubMed: 11805122] [Abstract] Cited for: PHOSPHORYLATION AT THR-321, INTERACTION WITH DLG1 AND DLG4, MUTAGENESIS OF THR-321 AND VAL-323.
[3]	"Microtubule-associated protein light chain 2 is a stargazin-AMPA receptor complex-interacting protein in vivo." Ives J.H., Fung S., Tiwari P., Payne H.L., Thompson C.L. J. Biol. Chem. 279:31002-31009(2004) [PubMed: 15136571] [Abstract] Cited for: INTERACTION WITH GOPC.
[4]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-271, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF077739 mRNA. Translation: AAC40201.1.
IPI	IPI00132786.
RefSeq	NP_031609.1. NM_007583.2.
UniGene	Mm.277338. Mm.400802.
3D structure databases
ProteinModelPortal	O88602.
ModBase	Search...
Protein-protein interaction databases
IntAct	O88602. 2 interactions.
STRING	O88602.
PTM databases
PhosphoSite	O88602.
Proteomic databases
PRIDE	O88602.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000019290; ENSMUSP00000019290; ENSMUSG00000019146.
GeneID	12300.
KEGG	mmu:12300.
Organism-specific databases
CTD	10369.
MGI	MGI:1316660. Cacng2.
Phylogenomic databases
eggNOG	roNOG06471.
HOGENOM	HBG564649.
HOVERGEN	HBG003682.
InParanoid	O88602.
OMA	ANARATD.
OrthoDB	EOG4P5K9M.
PhylomeDB	O88602.
Gene expression databases
ArrayExpress	O88602.
Bgee	O88602.
Genevestigator	O88602.
GermOnline	ENSMUSG00000019146. Mus musculus.
Family and domain databases
InterPro	IPR004031. PMP22/EMP/MP20/Claudin. IPR005422. VDCC_g2su. IPR008368. VDCC_gsu. [Graphical view]
KO	K04867.
Pfam	PF00822. PMP22_Claudin. 1 hit. [Graphical view]
PRINTS	PR01792. VDCCGAMMA. PR01602. VDCCGAMMA2.
ProtoNet	Search...
Other
NextBio	280822.
SOURCE	Search...

Entry information

Entry name

CCG2_MOUSE

Accession

Primary (citable) accession number: O88602

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents