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O88600 (HSP74_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat shock 70 kDa protein 4
Alternative name(s):
Ischemia responsive 94 kDa protein
Gene names
Name:Hspa4
Synonyms:Irp94
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with TJP1/ZO-1 By similarity.

Subcellular location

Cytoplasm Probable.

Tissue specificity

Ubiquitous. Highly expressed in testis. Ref.1

Induction

Up-regulated in neuronal cells upon ischemia. Ref.1

Sequence similarities

Belongs to the heat shock protein 70 family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processkidney development

Inferred from expression pattern PubMed 21938673. Source: RGD

negative regulation of DNA binding

Inferred from mutant phenotype PubMed 17473852. Source: RGD

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 21521685. Source: RGD

negative regulation of cell death

Inferred from mutant phenotype PubMed 17473852. Source: RGD

negative regulation of gene expression

Inferred from mutant phenotype PubMed 17473852. Source: RGD

negative regulation of protein phosphorylation

Inferred from mutant phenotype PubMed 17473852. Source: RGD

positive regulation of angiogenesis

Inferred from expression pattern PubMed 22098710. Source: RGD

positive regulation of gene expression

Inferred from mutant phenotype PubMed 21521685. Source: RGD

positive regulation of protein binding

Inferred from direct assay PubMed 11415446. Source: RGD

response to heat

Inferred from direct assay Ref.1. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 21861342. Source: RGD

cytosol

Inferred from direct assay PubMed 11415446PubMed 17175194. Source: RGD

lipid particle

Inferred from direct assay PubMed 17175194. Source: RGD

nucleus

Inferred from direct assay PubMed 21861342. Source: RGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA polymerase II transcription factor binding

Inferred from physical interaction PubMed 17473852. Source: RGD

activating transcription factor binding

Inferred from physical interaction PubMed 21521685PubMed 22003111. Source: RGD

protein binding

Inferred from physical interaction PubMed 17473852PubMed 22143029. Source: RGD

protein complex binding

Inferred from physical interaction PubMed 21561597. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Heat shock 70 kDa protein 4
PRO_0000255934

Amino acid modifications

Modified residue531N6-acetyllysine By similarity
Modified residue761Phosphoserine By similarity
Modified residue891Phosphotyrosine By similarity
Modified residue3361Phosphotyrosine By similarity
Modified residue4301N6-acetyllysine By similarity
Modified residue5381Phosphothreonine By similarity
Modified residue5461Phosphoserine By similarity
Modified residue6601Phosphotyrosine By similarity

Experimental info

Sequence conflict627 – 6282EM → DF AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
O88600 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 77BC00171669BF71

FASTA84094,057
        10         20         30         40         50         60 
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSVGA AAKSQVISNA 

        70         80         90        100        110        120 
KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM 

       130        140        150        160        170        180 
LLSKLKETAE SVLKKPVVDC VVSVPSFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA 

       190        200        210        220        230        240 
YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL 

       250        260        270        280        290        300 
VNHFCEEFGK KYKLDIKSKV RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT 

       310        320        330        340        350        360 
MNRGKFLEMC DDLLARVEPP LRSILDQSKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK 

       370        380        390        400        410        420 
ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF 

       430        440        450        460        470        480 
PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV 

       490        500        510        520        530        540 
KVRVNVHGIF SVSSAALVEV HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQPQTPA 

       550        560        570        580        590        600 
ENKAESEEME TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IESQLLWQLD REMLGLYTEN 

       610        620        630        640        650        660 
EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNNFTL KLEDTENWLY 

       670        680        690        700        710        720 
EDGEDQPKQV YVDKLAELRT LGQPIKTRFQ ESEERPKLFE ELGKQIQQYM KVISSFKNKE 

       730        740        750        760        770        780 
DQYEHLDAAD MTKVEKSTNE AMEWMNSKLN LQNKQSLTAD PVVKTKEIEA KIKELTNICS 

       790        800        810        820        830        840 
PIISKPKPKV EPPKEEPKHA EQNGPVDGQG DNPGTQAAEH GADTAVPSDG DKKLPEMDID 

« Hide

References

[1]"Molecular cloning of a novel member of the HSP110 family of genes, ischemia-responsive protein 94 kDa (irp94), expressed in rat brain after transient forebrain ischemia."
Yagita Y., Kitagawa K., Taguchi A., Ohtsuki T., Kuwabara K., Mabuchi T., Matsumoto M., Yanagihara T., Hori M.
J. Neurochem. 72:1544-1551(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[2]Lubec G., Chen W.-Q., Afjehi-Sadat L.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 391-405; 574-591 AND 619-629, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077354 mRNA. Translation: AAC27937.1.
RefSeqNP_705893.1. NM_153629.1.
UniGeneRn.163092.

3D structure databases

ProteinModelPortalO88600.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid251801. 11 interactions.
IntActO88600. 1 interaction.
MINTMINT-4566747.
STRING10116.ENSRNOP00000023628.

PTM databases

PhosphoSiteO88600.

2D gel databases

World-2DPAGE0004:O88600.

Proteomic databases

PaxDbO88600.
PRIDEO88600.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID266759.
KEGGrno:266759.
UCSCRGD:628878. rat.

Organism-specific databases

CTD3308.
RGD628878. Hspa4.

Phylogenomic databases

eggNOGCOG0443.
HOGENOMHOG000228138.
HOVERGENHBG047955.
InParanoidO88600.
KOK09489.
PhylomeDBO88600.

Gene expression databases

GenevestigatorO88600.

Family and domain databases

Gene3D1.20.1270.10. 1 hit.
2.60.34.10. 2 hits.
InterProIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 2 hits.
SSF100934. SSF100934. 2 hits.
PROSITEPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624599.
PROO88600.

Entry information

Entry nameHSP74_RAT
AccessionPrimary (citable) accession number: O88600
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families