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Protein

Heat shock 70 kDa protein 4

Gene

Hspa4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • activating transcription factor binding Source: RGD
  • ATP binding Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • RNA polymerase II transcription factor binding Source: RGD

GO - Biological processi

  • kidney development Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cell death Source: RGD
  • negative regulation of DNA binding Source: RGD
  • negative regulation of gene expression Source: RGD
  • negative regulation of protein phosphorylation Source: RGD
  • positive regulation of angiogenesis Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of protein binding Source: RGD
  • response to heat Source: RGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 4
Alternative name(s):
Ischemia responsive 94 kDa protein
Gene namesi
Name:Hspa4
Synonyms:Irp94
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628878. Hspa4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • lipid particle Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002559341 – 840Heat shock 70 kDa protein 4Add BLAST840

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei76PhosphoserineCombined sources1
Modified residuei89PhosphotyrosineBy similarity1
Modified residuei336PhosphotyrosineBy similarity1
Modified residuei393PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei430N6-acetyllysineBy similarity1
Modified residuei538PhosphothreonineCombined sources1
Modified residuei546PhosphoserineBy similarity1
Modified residuei660PhosphotyrosineBy similarity1
Modified residuei756PhosphoserineBy similarity1
Modified residuei773N6-methyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiO88600.
PRIDEiO88600.

2D gel databases

World-2DPAGE0004:O88600.

PTM databases

iPTMnetiO88600.
PhosphoSitePlusiO88600.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in testis.1 Publication

Inductioni

Up-regulated in neuronal cells upon ischemia.1 Publication

Interactioni

Subunit structurei

Interacts with TJP1/ZO-1.By similarity

GO - Molecular functioni

  • activating transcription factor binding Source: RGD
  • protein complex binding Source: RGD
  • RNA polymerase II transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi251801. 15 interactors.
IntActiO88600. 1 interactor.
MINTiMINT-4566747.
STRINGi10116.ENSRNOP00000023628.

Structurei

3D structure databases

ProteinModelPortaliO88600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0103. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228138.
HOVERGENiHBG047955.
InParanoidiO88600.
KOiK09489.
PhylomeDBiO88600.

Family and domain databases

Gene3Di1.20.1270.10. 2 hits.
2.60.34.10. 2 hits.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 2 hits.
SSF100934. SSF100934. 2 hits.
PROSITEiPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSVGA
60 70 80 90 100
AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG
110 120 130 140 150
IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPSFYTD
160 170 180 190 200
AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF
210 220 230 240 250
VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK
260 270 280 290 300
KYKLDIKSKV RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT
310 320 330 340 350
MNRGKFLEMC DDLLARVEPP LRSILDQSKL KKEDIYAVEI VGGATRIPAV
360 370 380 390 400
KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY
410 420 430 440 450
PISLRWNSPA EEGSSDCEVF PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ
460 470 480 490 500
DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSAALVEV
510 520 530 540 550
HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQPQTPA ENKAESEEME
560 570 580 590 600
TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IESQLLWQLD REMLGLYTEN
610 620 630 640 650
EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNNFTL
660 670 680 690 700
KLEDTENWLY EDGEDQPKQV YVDKLAELRT LGQPIKTRFQ ESEERPKLFE
710 720 730 740 750
ELGKQIQQYM KVISSFKNKE DQYEHLDAAD MTKVEKSTNE AMEWMNSKLN
760 770 780 790 800
LQNKQSLTAD PVVKTKEIEA KIKELTNICS PIISKPKPKV EPPKEEPKHA
810 820 830 840
EQNGPVDGQG DNPGTQAAEH GADTAVPSDG DKKLPEMDID
Length:840
Mass (Da):94,057
Last modified:November 1, 1998 - v1
Checksum:i77BC00171669BF71
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti627 – 628EM → DF AA sequence (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077354 mRNA. Translation: AAC27937.1.
RefSeqiNP_705893.1. NM_153629.1.
UniGeneiRn.163092.

Genome annotation databases

GeneIDi266759.
KEGGirno:266759.
UCSCiRGD:628878. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077354 mRNA. Translation: AAC27937.1.
RefSeqiNP_705893.1. NM_153629.1.
UniGeneiRn.163092.

3D structure databases

ProteinModelPortaliO88600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251801. 15 interactors.
IntActiO88600. 1 interactor.
MINTiMINT-4566747.
STRINGi10116.ENSRNOP00000023628.

PTM databases

iPTMnetiO88600.
PhosphoSitePlusiO88600.

2D gel databases

World-2DPAGE0004:O88600.

Proteomic databases

PaxDbiO88600.
PRIDEiO88600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266759.
KEGGirno:266759.
UCSCiRGD:628878. rat.

Organism-specific databases

CTDi3308.
RGDi628878. Hspa4.

Phylogenomic databases

eggNOGiKOG0103. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228138.
HOVERGENiHBG047955.
InParanoidiO88600.
KOiK09489.
PhylomeDBiO88600.

Miscellaneous databases

ChiTaRSiHspa4. rat.
PROiO88600.

Family and domain databases

Gene3Di1.20.1270.10. 2 hits.
2.60.34.10. 2 hits.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 2 hits.
SSF100934. SSF100934. 2 hits.
PROSITEiPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSP74_RAT
AccessioniPrimary (citable) accession number: O88600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.