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O88600

- HSP74_RAT

UniProt

O88600 - HSP74_RAT

Protein

Heat shock 70 kDa protein 4

Gene

Hspa4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. activating transcription factor binding Source: RGD
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: RGD
    4. protein complex binding Source: RGD
    5. RNA polymerase II transcription factor binding Source: RGD

    GO - Biological processi

    1. kidney development Source: RGD
    2. negative regulation of apoptotic process Source: RGD
    3. negative regulation of cell death Source: RGD
    4. negative regulation of DNA binding Source: RGD
    5. negative regulation of gene expression Source: RGD
    6. negative regulation of protein phosphorylation Source: RGD
    7. positive regulation of angiogenesis Source: RGD
    8. positive regulation of gene expression Source: RGD
    9. positive regulation of protein binding Source: RGD
    10. response to heat Source: RGD

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock 70 kDa protein 4
    Alternative name(s):
    Ischemia responsive 94 kDa protein
    Gene namesi
    Name:Hspa4
    Synonyms:Irp94
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi628878. Hspa4.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytosol Source: RGD
    3. lipid particle Source: RGD
    4. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 840840Heat shock 70 kDa protein 4PRO_0000255934Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531N6-acetyllysineBy similarity
    Modified residuei76 – 761PhosphoserineBy similarity
    Modified residuei89 – 891PhosphotyrosineBy similarity
    Modified residuei336 – 3361PhosphotyrosineBy similarity
    Modified residuei430 – 4301N6-acetyllysineBy similarity
    Modified residuei538 – 5381PhosphothreonineBy similarity
    Modified residuei546 – 5461PhosphoserineBy similarity
    Modified residuei660 – 6601PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiO88600.
    PRIDEiO88600.

    2D gel databases

    World-2DPAGE0004:O88600.

    PTM databases

    PhosphoSiteiO88600.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highly expressed in testis.1 Publication

    Inductioni

    Up-regulated in neuronal cells upon ischemia.1 Publication

    Gene expression databases

    GenevestigatoriO88600.

    Interactioni

    Subunit structurei

    Interacts with TJP1/ZO-1.By similarity

    Protein-protein interaction databases

    BioGridi251801. 11 interactions.
    IntActiO88600. 1 interaction.
    MINTiMINT-4566747.
    STRINGi10116.ENSRNOP00000023628.

    Structurei

    3D structure databases

    ProteinModelPortaliO88600.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Phylogenomic databases

    eggNOGiCOG0443.
    HOGENOMiHOG000228138.
    HOVERGENiHBG047955.
    InParanoidiO88600.
    KOiK09489.
    PhylomeDBiO88600.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 2 hits.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 2 hits.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 2 hits.
    SSF100934. SSF100934. 2 hits.
    PROSITEiPS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88600-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSVGA    50
    AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG 100
    IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPSFYTD 150
    AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF 200
    VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK 250
    KYKLDIKSKV RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT 300
    MNRGKFLEMC DDLLARVEPP LRSILDQSKL KKEDIYAVEI VGGATRIPAV 350
    KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY 400
    PISLRWNSPA EEGSSDCEVF PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ 450
    DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSAALVEV 500
    HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQPQTPA ENKAESEEME 550
    TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IESQLLWQLD REMLGLYTEN 600
    EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNNFTL 650
    KLEDTENWLY EDGEDQPKQV YVDKLAELRT LGQPIKTRFQ ESEERPKLFE 700
    ELGKQIQQYM KVISSFKNKE DQYEHLDAAD MTKVEKSTNE AMEWMNSKLN 750
    LQNKQSLTAD PVVKTKEIEA KIKELTNICS PIISKPKPKV EPPKEEPKHA 800
    EQNGPVDGQG DNPGTQAAEH GADTAVPSDG DKKLPEMDID 840
    Length:840
    Mass (Da):94,057
    Last modified:November 1, 1998 - v1
    Checksum:i77BC00171669BF71
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti627 – 6282EM → DF AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077354 mRNA. Translation: AAC27937.1.
    RefSeqiNP_705893.1. NM_153629.1.
    UniGeneiRn.163092.

    Genome annotation databases

    GeneIDi266759.
    KEGGirno:266759.
    UCSCiRGD:628878. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077354 mRNA. Translation: AAC27937.1 .
    RefSeqi NP_705893.1. NM_153629.1.
    UniGenei Rn.163092.

    3D structure databases

    ProteinModelPortali O88600.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251801. 11 interactions.
    IntActi O88600. 1 interaction.
    MINTi MINT-4566747.
    STRINGi 10116.ENSRNOP00000023628.

    PTM databases

    PhosphoSitei O88600.

    2D gel databases

    World-2DPAGE 0004:O88600.

    Proteomic databases

    PaxDbi O88600.
    PRIDEi O88600.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 266759.
    KEGGi rno:266759.
    UCSCi RGD:628878. rat.

    Organism-specific databases

    CTDi 3308.
    RGDi 628878. Hspa4.

    Phylogenomic databases

    eggNOGi COG0443.
    HOGENOMi HOG000228138.
    HOVERGENi HBG047955.
    InParanoidi O88600.
    KOi K09489.
    PhylomeDBi O88600.

    Miscellaneous databases

    NextBioi 624599.
    PROi O88600.

    Gene expression databases

    Genevestigatori O88600.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 2 hits.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 2 hits.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 2 hits.
    SSF100934. SSF100934. 2 hits.
    PROSITEi PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a novel member of the HSP110 family of genes, ischemia-responsive protein 94 kDa (irp94), expressed in rat brain after transient forebrain ischemia."
      Yagita Y., Kitagawa K., Taguchi A., Ohtsuki T., Kuwabara K., Mabuchi T., Matsumoto M., Yanagihara T., Hori M.
      J. Neurochem. 72:1544-1551(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    2. Lubec G., Chen W.-Q., Afjehi-Sadat L.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 391-405; 574-591 AND 619-629, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiHSP74_RAT
    AccessioniPrimary (citable) accession number: O88600
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3