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Protein

Phosphofurin acidic cluster sorting protein 1

Gene

Pacs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits.

GO - Molecular functioni

GO - Biological processi

  • cellular protein localization Source: RGD
  • positive regulation of protein binding Source: RGD
  • protein targeting to plasma membrane Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphofurin acidic cluster sorting protein 1
Short name:
PACS-1
Gene namesi
Name:Pacs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620579. Pacs1.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 961960Phosphofurin acidic cluster sorting protein 1PRO_0000058173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei28 – 281PhosphoserineBy similarity
Modified residuei44 – 441PhosphothreonineBy similarity
Modified residuei249 – 2491PhosphotyrosineBy similarity
Modified residuei377 – 3771PhosphoserineCombined sources
Modified residuei379 – 3791PhosphoserineCombined sources
Modified residuei428 – 4281PhosphoserineBy similarity
Modified residuei493 – 4931PhosphoserineBy similarity
Modified residuei502 – 5021PhosphothreonineBy similarity
Modified residuei517 – 5171PhosphoserineBy similarity
Modified residuei526 – 5261PhosphoserineCombined sources
Modified residuei527 – 5271PhosphoserineCombined sources
Modified residuei529 – 5291PhosphoserineBy similarity
Modified residuei532 – 5321PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO88588.
PRIDEiO88588.

PTM databases

iPTMnetiO88588.
PhosphoSiteiO88588.

Interactioni

Subunit structurei

Associatews with AP-1 and AP-3 but not with AP-2 complexes. Forms a ternary complex with furin and AP-1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO88588. 1 interaction.
MINTiMINT-3372040.
STRINGi10116.ENSRNOP00000027632.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili351 – 37525Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 8230Ser-richAdd
BLAST
Compositional biasi60 – 634Poly-Ala
Compositional biasi110 – 1134Poly-Ser
Compositional biasi277 – 2815Poly-Glu

Sequence similaritiesi

Belongs to the PACS family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3709. Eukaryota.
ENOG410XQNV. LUCA.
HOGENOMiHOG000047329.
HOVERGENiHBG053488.
InParanoidiO88588.
PhylomeDBiO88588.

Family and domain databases

InterProiIPR019381. Phosphofurin_acidic_CS-1.
[Graphical view]
PfamiPF10254. Pacs-1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform PACS-1a (identifier: O88588-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAERGGAGGG PGGAGGGSSQ RGSGVAQSPQ QQPPQQPSQP QQPTPPKLAQ
60 70 80 90 100
ATSSSSSTSA AAASSSSSST STSMAVAVAS GSAPPGGPGP GRTPAPVQMN
110 120 130 140 150
LYATWEVDRS SSSCVPRLFS LTLKKLVMLK EMDKDLNSVV IAVKLQGSKR
160 170 180 190 200
ILRSNEIILP ASGLVETELQ LTFSLQYPHF LKRDANKLQI MLQRRKRYKN
210 220 230 240 250
RTILGYKTLA VGLINMAEVM QHPNEGALVL GLHSNVKDVS VPVAEIKIYS
260 270 280 290 300
LSSQPIDHEG IKSKLSDRSP DIDNYSEEEE ESFSSEQEGS DDPLHGQDLF
310 320 330 340 350
YEDEDLRKVK KTRRKLTSTS AITRQPNIKQ KFVALLKRFK VSDEVGFGLE
360 370 380 390 400
HVSREQIREV EEDLDELYDS LEMYNPSDSG PEMEETESIL STPKPKLKPF
410 420 430 440 450
FEGMSQSSSQ TEIGSLNSKG SLGKDTTSPM ELAALEKVKS TWIKNQDDSL
460 470 480 490 500
TETDTLEITD QDMFGDASTS LVVPEKVKTP MKSSKADLQG SASPSKVEGT
510 520 530 540 550
HTPRQKRSTP LKERQLSKPL SERTNSSDSE RSPDLGHSTQ IPRKVVYDQL
560 570 580 590 600
NQILVSDAAL PENVILVNTT DWQGQYVAEL LQDQRKPVVC TCSTVEVQAV
610 620 630 640 650
LSALLTRIQR YCNCNSSMPR PVKVAAVGSQ SYLSSILRFF VKSLASKTPD
660 670 680 690 700
WLGHMRFLIV PLGSHPVAKY LGSVDSRYSS TFLDSAWRDL FSRSEPPVSE
710 720 730 740 750
PLDVVGRVMQ YVNGATTTHQ LPVAEAMLTC RHKFPDEDSY QKFIPFIGVV
760 770 780 790 800
KVGLVEDSPS TAGDGDDSPV VSLTVPSTSP PSSSGLSRDA TATPPSSPSM
810 820 830 840 850
SSALAIVGSP NSPYGDVIGL QVDYWLGHPG ERRREGDKRD ASSKNTLKSV
860 870 880 890 900
FRSVQVSRLP HAGEAQLSGT MAMTVVTKEK NKKVPTIFLS KKPREKEVDS
910 920 930 940 950
KSQVIEGISR LICSAKQQQT MLRVSIDGVE WSDIKFFQLA AQWPTHVKHF
960
PVGLFSGSKP T
Length:961
Mass (Da):104,700
Last modified:November 1, 1998 - v1
Checksum:i9B4A4522DFF9DEAF
GO
Isoform PACS-1b (identifier: O88588-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     541-559: IPRKVVYDQLNQILVSDAA → VILGSSRFRTPFLDFSLFL
     560-961: Missing.

Show »
Length:559
Mass (Da):60,850
Checksum:i6D1D4CDF5EA2AB76
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei541 – 55919IPRKV…VSDAA → VILGSSRFRTPFLDFSLFL in isoform PACS-1b. 1 PublicationVSP_011558Add
BLAST
Alternative sequencei560 – 961402Missing in isoform PACS-1b. 1 PublicationVSP_011559Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076183 mRNA. Translation: AAC31815.1.
AF076184 mRNA. Translation: AAC31816.1.
RefSeqiNP_599233.1. NM_134406.1. [O88588-1]
UniGeneiRn.154425.

Genome annotation databases

GeneIDi171444.
KEGGirno:171444.
UCSCiRGD:620579. rat. [O88588-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076183 mRNA. Translation: AAC31815.1.
AF076184 mRNA. Translation: AAC31816.1.
RefSeqiNP_599233.1. NM_134406.1. [O88588-1]
UniGeneiRn.154425.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88588. 1 interaction.
MINTiMINT-3372040.
STRINGi10116.ENSRNOP00000027632.

PTM databases

iPTMnetiO88588.
PhosphoSiteiO88588.

Proteomic databases

PaxDbiO88588.
PRIDEiO88588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171444.
KEGGirno:171444.
UCSCiRGD:620579. rat. [O88588-1]

Organism-specific databases

CTDi55690.
RGDi620579. Pacs1.

Phylogenomic databases

eggNOGiKOG3709. Eukaryota.
ENOG410XQNV. LUCA.
HOGENOMiHOG000047329.
HOVERGENiHBG053488.
InParanoidiO88588.
PhylomeDBiO88588.

Miscellaneous databases

PROiO88588.

Family and domain databases

InterProiIPR019381. Phosphofurin_acidic_CS-1.
[Graphical view]
PfamiPF10254. Pacs-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPACS1_RAT
AccessioniPrimary (citable) accession number: O88588
Secondary accession number(s): O88589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.