##gff-version 3 O88576 UniProtKB Chain 1 615 . . . ID=PRO_0000214807;Note=Sodium-dependent neutral amino acid transporter B(0)AT3 O88576 UniProtKB Topological domain 1 26 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 27 47 . . . Note=Helical%3B Name%3D1;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 48 52 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 53 73 . . . Note=Helical%3B Name%3D2;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 74 105 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 106 126 . . . Note=Helical%3B Name%3D3;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 127 177 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 178 198 . . . Note=Helical%3B Name%3D4;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 199 206 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 207 227 . . . Note=Helical%3B Name%3D5;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 228 255 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 256 276 . . . Note=Helical%3B Name%3D6;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 277 288 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 289 309 . . . Note=Helical%3B Name%3D7;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 310 397 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 398 418 . . . Note=Helical%3B Name%3D8;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 419 441 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 442 462 . . . Note=Helical%3B Name%3D9;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 463 472 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 473 493 . . . Note=Helical%3B Name%3D10;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 494 520 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 521 541 . . . Note=Helical%3B Name%3D11;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 542 570 . . . Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Transmembrane 571 591 . . . Note=Helical%3B Name%3D12;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Topological domain 592 615 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Glycosylation 143 143 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00498 O88576 UniProtKB Glycosylation 167 167 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00498 O88576 UniProtKB Glycosylation 353 353 . . . Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255 O88576 UniProtKB Alternative sequence 377 444 . . . ID=VSP_050364;Note=In isoform 3 and isoform 5. SASGPGLAFIVFTEAVLHMPGASVWSVLFFGMLFTLGLSSMFGNMEGVITPLLDMGILPKGIPKEVMT->PTWKQISGARVLGEGCARLTSRVCEASVLP;Ontology_term=ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:15489334,ECO:0000303|PubMed:9932288;Dbxref=PMID:15489334,PMID:9932288 O88576 UniProtKB Alternative sequence 377 422 . . . ID=VSP_050363;Note=In isoform 6. SASGPGLAFIVFTEAVLHMPGASVWSVLFFGMLFTLGLSSMFGNME->VLLCGLCSSLGCCLPWVCPPCLGTWRVSLHHYWTWGSYPKVYPRRS;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9932288;Dbxref=PMID:9932288 O88576 UniProtKB Alternative sequence 423 615 . . . ID=VSP_050697;Note=In isoform 6. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9932288;Dbxref=PMID:9932288 O88576 UniProtKB Alternative sequence 445 454 . . . ID=VSP_050365;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9932288;Dbxref=PMID:9932288 O88576 UniProtKB Alternative sequence 499 552 . . . ID=VSP_050366;Note=In isoform 4 and isoform 5. FCDDIEWMTGRRPGLYWQVTWRVVSPMLLFGIFLSYIVLLIQTPPSYKAWNPQY->NIFPQERRSSTQAGCRSPVCSCPSCPHCGSLELLWLSYCPSTNRGGRLRIWKVV;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9932288;Dbxref=PMID:9932288 O88576 UniProtKB Alternative sequence 553 615 . . . ID=VSP_050367;Note=In isoform 4 and isoform 5. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9932288;Dbxref=PMID:9932288 O88576 UniProtKB Mutagenesis 21 21 . . . Note=No effect on protein abundance. Increases localization to the cell membrane. Increases alanine uptake activity. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 25 25 . . . Note=No effect on protein abundance. Almost complete loss of cell surface localization. Decreases alanine uptake activity. Q->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 25 25 . . . Note=No effect on protein abundance. Strong decrease of cell surface localization. Decreases alanine uptake. Q->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 50 50 . . . Note=No effect on protein abundance. No effect on cell surface localization. Increases alanine uptake activity. H->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 72 72 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. Y->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 78 78 . . . Note=No effect on protein abundance. No effect on cell surface expression. Decreases alanine uptake activity. G->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 90 90 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. K->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 158 158 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. S->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 213 213 . . . Note=No effect on protein abundance. No effect on cell surface localization. Decreases alanine uptake activity. L->W;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 225 225 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake. R->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 283 283 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake. N->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 287 287 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake. D->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 287 287 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake activity. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 296 296 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake activity. S->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 297 297 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. M->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 297 297 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. M->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 456 456 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. I->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 464 464 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. G->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 477 477 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. L->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 495 495 . . . Note=No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake activity. G->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 502 502 . . . Note=Decreases protein abundance. Strong decreases of cell surface localization. Strong decreases on alanine uptake activity. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152 O88576 UniProtKB Mutagenesis 568 568 . . . Note=No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity. Q->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:26240152;Dbxref=PMID:26240152