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Protein

Sodium-dependent neutral amino acid transporter B(0)AT3

Gene

Slc6a18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a sodium and chloride-dependent neutral amino acid transporter.

GO - Molecular functioni

  • neurotransmitter:sodium symporter activity Source: InterPro
  • transporter activity Source: MGI

GO - Biological processi

  • amino acid transmembrane transport Source: MGI
  • transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent neutral amino acid transporter B(0)AT3
Alternative name(s):
Sodium- and chloride-dependent transporter XTRP2
Solute carrier family 6 member 18
System B(0) neutral amino acid transporter AT3
Gene namesi
Name:Slc6a18
Synonyms:Xt2, Xtrp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1336892. Slc6a18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei27 – 4721Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini48 – 525ExtracellularSequence Analysis
Transmembranei53 – 7321Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini74 – 10532CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei106 – 12621Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini127 – 17751ExtracellularSequence AnalysisAdd
BLAST
Transmembranei178 – 19821Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini199 – 2068CytoplasmicSequence Analysis
Transmembranei207 – 22721Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini228 – 25528ExtracellularSequence AnalysisAdd
BLAST
Transmembranei256 – 27621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini277 – 28812CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei289 – 30921Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini310 – 39788ExtracellularSequence AnalysisAdd
BLAST
Transmembranei398 – 41821Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini419 – 44123CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei442 – 46221Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini463 – 47210ExtracellularSequence Analysis
Transmembranei473 – 49321Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini494 – 52027CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei521 – 54121Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini542 – 57029ExtracellularSequence AnalysisAdd
BLAST
Transmembranei571 – 59121Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini592 – 61524CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • brush border membrane Source: MGI
  • integral component of plasma membrane Source: InterPro
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Animals lacking this protein exhibit no gross abnormalities and grow to adulthood, although they do exhibit hypertension. The elevated blood pressure appears to be attributable to a decreased level of renal glycine. High-affinity renal reabsorption of glycine is eliminated and intrarenal glycine concentration is reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Sodium-dependent neutral amino acid transporter B(0)AT3PRO_0000214807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO88576.
PRIDEiO88576.

PTM databases

PhosphoSiteiO88576.

Expressioni

Tissue specificityi

Expressed predominantly in kidney.1 Publication

Gene expression databases

BgeeiO88576.
GenevisibleiO88576. MM.

Interactioni

Subunit structurei

Associates with TMEM27. TMEM27 association is required for functional expression in kidney.

Protein-protein interaction databases

DIPiDIP-60421N.
STRINGi10090.ENSMUSP00000022105.

Structurei

3D structure databases

ProteinModelPortaliO88576.
SMRiO88576. Positions 15-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000119044.
HOVERGENiHBG071421.
InParanoidiO88576.
KOiK05048.
OMAiPIQGERI.
OrthoDBiEOG793B71.
PhylomeDBiO88576.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88576-1) [UniParc]FASTAAdd to basket

Also known as: A12

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQASGMDPL VDIEDERPKW DNKLQYLLSC IGFAVGLGNI WRFPYLCQTH
60 70 80 90 100
GGGAFLIPYF IALVFEGIPL FYIELAIGQR LRRGSIGVWK TISPYLGGVG
110 120 130 140 150
LGCFSVSFLV SLYYNTVLLW VLWFFLNSFQ HPLPWSTCPL DLNRTGFVQE
160 170 180 190 200
CQSSGTVSYF WYRQTLNITS DISNTGTIQW KLFLCLVACW STVYLCVIRG
210 220 230 240 250
IESTGKVIYF TALFPYLVLT IFLIRGLTLP GATEGLIYLF TPNMKTLQNP
260 270 280 290 300
RVWLDAATQI FFSLSLAFGG HIAFASYNPP RNNCEKDAVI IALVNSMTSL
310 320 330 340 350
YASIAIFSVM GFKASNDYGR CLDRNILSLI NEFDLPELSI SRDEYPSVLM
360 370 380 390 400
YLNATQTARV AQLPLKTCHL EDFLDKSASG PGLAFIVFTE AVLHMPGASV
410 420 430 440 450
WSVLFFGMLF TLGLSSMFGN MEGVITPLLD MGILPKGIPK EVMTGVICFA
460 470 480 490 500
CFLSAICFTL QSGGYWLEIF DSFAASLNLI IFAFMEVVGV IHIYGMKRFC
510 520 530 540 550
DDIEWMTGRR PGLYWQVTWR VVSPMLLFGI FLSYIVLLIQ TPPSYKAWNP
560 570 580 590 600
QYEHFPSREE KFYPGWVQVT CVLLSFLPSL WVPGVALAQL LSQYKQRWKA
610
THLESGLKLQ ESRGC
Length:615
Mass (Da):69,229
Last modified:November 1, 1998 - v1
Checksum:iA7925C0397FC63F8
GO
Isoform 2 (identifier: O88576-2) [UniParc]FASTAAdd to basket

Also known as: A11

The sequence of this isoform differs from the canonical sequence as follows:
     445-454: Missing.

Show »
Length:605
Mass (Da):68,188
Checksum:iE86342EE242445BF
GO
Isoform 3 (identifier: O88576-3) [UniParc]FASTAAdd to basket

Also known as: B11

The sequence of this isoform differs from the canonical sequence as follows:
     377-444: SASGPGLAFI...PKGIPKEVMT → PTWKQISGARVLGEGCARLTSRVCEASVLP

Show »
Length:577
Mass (Da):65,254
Checksum:i5F84F7C5CF1B14E3
GO
Isoform 4 (identifier: O88576-4) [UniParc]FASTAAdd to basket

Also known as: A10

The sequence of this isoform differs from the canonical sequence as follows:
     499-552: FCDDIEWMTG...PSYKAWNPQY → NIFPQERRSS...GGRLRIWKVV
     553-615: Missing.

Show »
Length:552
Mass (Da):61,513
Checksum:i8DEE83992276B575
GO
Isoform 5 (identifier: O88576-5) [UniParc]FASTAAdd to basket

Also known as: B9

The sequence of this isoform differs from the canonical sequence as follows:
     377-444: SASGPGLAFI...PKGIPKEVMT → PTWKQISGARVLGEGCARLTSRVCEASVLP
     499-552: FCDDIEWMTG...PSYKAWNPQY → NIFPQERRSS...GGRLRIWKVV
     553-615: Missing.

Show »
Length:514
Mass (Da):57,538
Checksum:i3743C2342ECE0610
GO
Isoform 6 (identifier: O88576-6) [UniParc]FASTAAdd to basket

Also known as: A8

The sequence of this isoform differs from the canonical sequence as follows:
     377-422: SASGPGLAFI...GLSSMFGNME → VLLCGLCSSL...SYPKVYPRRS
     423-615: Missing.

Show »
Length:422
Mass (Da):47,619
Checksum:i6A794BDE016C00C3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei377 – 44468SASGP…KEVMT → PTWKQISGARVLGEGCARLT SRVCEASVLP in isoform 3 and isoform 5. 2 PublicationsVSP_050364Add
BLAST
Alternative sequencei377 – 42246SASGP…FGNME → VLLCGLCSSLGCCLPWVCPP CLGTWRVSLHHYWTWGSYPK VYPRRS in isoform 6. 1 PublicationVSP_050363Add
BLAST
Alternative sequencei423 – 615193Missing in isoform 6. 1 PublicationVSP_050697Add
BLAST
Alternative sequencei445 – 45410Missing in isoform 2. 1 PublicationVSP_050365
Alternative sequencei499 – 55254FCDDI…WNPQY → NIFPQERRSSTQAGCRSPVC SCPSCPHCGSLELLWLSYCP STNRGGRLRIWKVV in isoform 4 and isoform 5. 1 PublicationVSP_050366Add
BLAST
Alternative sequencei553 – 61563Missing in isoform 4 and isoform 5. 1 PublicationVSP_050367Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075262 mRNA. Translation: AAC27757.1.
AF075263 mRNA. Translation: AAC27758.1.
AF075264 mRNA. Translation: AAC27759.1.
AF075265 mRNA. Translation: AAC27760.1.
AF075266 mRNA. Translation: AAC27761.1.
AF075267 mRNA. Translation: AAC27762.1.
BC010748 mRNA. Translation: AAH10748.1.
CCDSiCCDS36727.1. [O88576-1]
CCDS49311.1. [O88576-3]
CCDS49312.1. [O88576-4]
RefSeqiNP_001035782.1. NM_001040692.3. [O88576-1]
NP_001129559.1. NM_001136087.2. [O88576-3]
NP_001162115.1. NM_001168644.1. [O88576-4]
NP_001162116.1. NM_001168645.1. [O88576-5]
NP_001162117.1. NM_001168646.1. [O88576-2]
UniGeneiMm.30370.

Genome annotation databases

EnsembliENSMUST00000022105; ENSMUSP00000022105; ENSMUSG00000021612. [O88576-1]
ENSMUST00000109679; ENSMUSP00000105301; ENSMUSG00000021612. [O88576-4]
ENSMUST00000109680; ENSMUSP00000105302; ENSMUSG00000021612. [O88576-3]
GeneIDi22598.
KEGGimmu:22598.
UCSCiuc007rds.2. mouse. [O88576-1]
uc007rdt.2. mouse. [O88576-2]
uc007rdu.2. mouse. [O88576-3]
uc007rdv.2. mouse. [O88576-4]
uc007rdw.2. mouse. [O88576-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075262 mRNA. Translation: AAC27757.1.
AF075263 mRNA. Translation: AAC27758.1.
AF075264 mRNA. Translation: AAC27759.1.
AF075265 mRNA. Translation: AAC27760.1.
AF075266 mRNA. Translation: AAC27761.1.
AF075267 mRNA. Translation: AAC27762.1.
BC010748 mRNA. Translation: AAH10748.1.
CCDSiCCDS36727.1. [O88576-1]
CCDS49311.1. [O88576-3]
CCDS49312.1. [O88576-4]
RefSeqiNP_001035782.1. NM_001040692.3. [O88576-1]
NP_001129559.1. NM_001136087.2. [O88576-3]
NP_001162115.1. NM_001168644.1. [O88576-4]
NP_001162116.1. NM_001168645.1. [O88576-5]
NP_001162117.1. NM_001168646.1. [O88576-2]
UniGeneiMm.30370.

3D structure databases

ProteinModelPortaliO88576.
SMRiO88576. Positions 15-592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60421N.
STRINGi10090.ENSMUSP00000022105.

PTM databases

PhosphoSiteiO88576.

Proteomic databases

PaxDbiO88576.
PRIDEiO88576.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022105; ENSMUSP00000022105; ENSMUSG00000021612. [O88576-1]
ENSMUST00000109679; ENSMUSP00000105301; ENSMUSG00000021612. [O88576-4]
ENSMUST00000109680; ENSMUSP00000105302; ENSMUSG00000021612. [O88576-3]
GeneIDi22598.
KEGGimmu:22598.
UCSCiuc007rds.2. mouse. [O88576-1]
uc007rdt.2. mouse. [O88576-2]
uc007rdu.2. mouse. [O88576-3]
uc007rdv.2. mouse. [O88576-4]
uc007rdw.2. mouse. [O88576-5]

Organism-specific databases

CTDi348932.
MGIiMGI:1336892. Slc6a18.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000119044.
HOVERGENiHBG071421.
InParanoidiO88576.
KOiK05048.
OMAiPIQGERI.
OrthoDBiEOG793B71.
PhylomeDBiO88576.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

NextBioi302949.
PROiO88576.
SOURCEiSearch...

Gene expression databases

BgeeiO88576.
GenevisibleiO88576. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, gene structure, and genomic localization of an orphan transporter from mouse kidney with six alternatively-spliced isoforms."
    Nash S.R., Giros B., Kingsmore S.F., Kim K.M., El-Mestikawy S., Dong Q., Fumagalli F., Seldin M.F., Caron M.G.
    Recept. Channels 6:113-128(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6), TISSUE SPECIFICITY.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Kidney.
  3. "Hypertension and impaired glycine handling in mice lacking the orphan transporter XT2."
    Quan H., Athirakul K., Wetsel W.C., Torres G.E., Stevens R., Chen Y.T., Coffman T.M., Caron M.G.
    Mol. Cell. Biol. 24:4166-4173(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. Cited for: PROBABLE FUNCTION, ASSOCIATION WITH TMEM27.

Entry informationi

Entry nameiS6A18_MOUSE
AccessioniPrimary (citable) accession number: O88576
Secondary accession number(s): O88577
, O88578, O88579, O88580, O88581, Q91XG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: November 1, 1998
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.