Reviewed, UniProtKB/Swiss-Prot O88574 (SAP30_MOUSE)

Names and origin

Protein names

Recommended name:
    Histone deacetylase complex subunit SAP30
Alternative name(s):
    Sin3-associated polypeptide, 30 kDa
    Sin3 corepressor complex subunit SAP30

Gene names

Name:

Sap30

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. Ref.1 UniProtKB O75446
Subunit structure	Interacts with HCFC1 By similarity. A component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2. Interacts with SIN3A, SIN3B, HDAC1, HDAC2, RBBP4 and NCOR1. Ref.1 UniProtKB O75446
Subcellular location	Nucleus.
Sequence similarities	Belongs to the SAP30 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hdac1	O09106	1	EBI-593511,EBI-301912
Hdac2	P70288	1	EBI-593511,EBI-302251
Ncor1	Q60974	3	EBI-593511,EBI-349004
Rbbp4	Q60972	1	EBI-593511,EBI-302291
Sin3a	Q60520	5	EBI-593511,EBI-349034
Sin3b	Q62141	1	EBI-593511,EBI-591450

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Histone deacetylase complex subunit SAP30		PRO_0000097583
Regions
	Region	1 – 129	129	Interaction with NCOR1
	Region	130 – 220	91	Interaction with SIN3A
	Compositional bias	15 – 59	45	Ala-rich
Amino acid modifications
	Modified residue	104	1	N6-acetyllysine By similarity
	Modified residue	131	1	Phosphoserine By similarity
	Modified residue	138	1	Phosphoserine By similarity
	Modified residue	145	1	Phosphothreonine By similarity
	Modified residue	169	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O88574-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A9D4CBDAB97BBD8C	FASTA	220	23,231
	10 20 30 40 50 60 MNGFTPEEMS RGGDAAAAVA AVVAAAAAAA SAGNGNAAGG GAEVPGAGAV SASGPPGAAG 70 80 90 100 110 120 PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI ELDKSARHLY ICDYHKNLIQ 130 140 150 160 170 180 SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE 190 200 210 220 IVGCHFKSIP VNEKDTLTCF IYSVRNDKNK SDLKADSGVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors." Laherty C.D., Billin A.N., Lavinsky R.M., Yochum G.S., Bush A.C., Sun J.-M., Mullen T.-M., Davie J.R., Rose D.W., Glass C.K., Rosenfeld M.G., Ayer D.E., Eisenman R.N. Mol. Cell 2:33-42(1998) [PubMed: 9702189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NCOR1; SIN3A; SIN3B; HDAC1; HDAC2 AND RBBP4.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Liver and Thymus.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Mouse scrapie responsive gene 1 (Scrg1): genomic organization, physical linkage to sap30, genetic mapping on chromosome 8, and expression in neuronal primary cell cultures." Dron M., Tartare X., Guillo F., Haik S., Barbin G., Maury C., Tovey M., Dandoy-Dron F. Genomics 70:140-149(2000) [PubMed: 11087671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 181-220. Strain: C57BL/6N. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF075136 mRNA. Translation: AAC26007.1. AK010928 mRNA. Translation: BAB27273.1. AK088745 mRNA. Translation: BAC40543.1. CH466569 Genomic DNA. Translation: EDL28616.1. BC132081 mRNA. Translation: AAI32082.1. BC132087 mRNA. Translation: AAI32088.1. AJ251216 Genomic DNA. Translation: CAC24848.1.
IPI	IPI00132028.
RefSeq	NP_068560.1.
UniGene	Mm.118
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O88574. 6 interactions.
STRING	O88574.
PTM databases
PhosphoSite	O88574.
Proteomic databases
PRIDE	O88574.
Genome annotation databases
Ensembl	ENSMUST00000034022; ENSMUSP00000034022; ENSMUSG00000031609; Mus musculus. [Genome view]
GeneID	60406.
KEGG	mmu:60406.
UCSC	uc009lsu.1. mouse.
Organism-specific databases
CTD	60406.
MGI	MGI:1929129. Sap30.
Phylogenomic databases
HOGENOM	O88574.
HOVERGEN	O88574.
OMA	FTPDEMS
OrthoDB	EOG934ZRN
Gene expression databases
ArrayExpress	O88574.
Bgee	O88574.
Genevestigator	O88574.
GermOnline	ENSMUSG00000031609. Mus musculus.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	314849.
SOURCE	Search...

Entry information

Entry name

SAP30_MOUSE

Accession

Primary (citable) accession number: O88574
Secondary accession number(s): A2RSE9, Q99JB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	November 1, 1998
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)