Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histone deacetylase complex subunit SAP30

Gene

Sap30

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri67 – 11549AtypicalAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. transcription corepressor activity Source: MGI

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  2. regulation of transcription, DNA-templated Source: MGI
  3. skeletal muscle cell differentiation Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_257106. HDACs deacetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP30
Alternative name(s):
30 kDa Sin3-associated polypeptide
Sin3 corepressor complex subunit SAP30
Sin3-associated polypeptide p30
Gene namesi
Name:Sap30Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1929129. Sap30.

Subcellular locationi

GO - Cellular componenti

  1. histone deacetylase complex Source: MGI
  2. nucleus Source: MGI
  3. Sin3 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Histone deacetylase complex subunit SAP30PRO_0000097583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311Phosphoserine1 Publication
Modified residuei138 – 1381PhosphoserineBy similarity
Modified residuei145 – 1451PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88574.
PRIDEiO88574.

PTM databases

PhosphoSiteiO88574.

Expressioni

Gene expression databases

BgeeiO88574.
GenevestigatoriO88574.

Interactioni

Subunit structurei

Interacts with HCFC1 (By similarity). A component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2. Interacts with SIN3A, SIN3B, HDAC1, HDAC2, RBBP4 and NCOR1. Interacts directly with SAMSN1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ncor1Q609743EBI-593511,EBI-349004
Sin3aQ605206EBI-593511,EBI-349034

Protein-protein interaction databases

BioGridi208559. 4 interactions.
IntActiO88574. 7 interactions.
MINTiMINT-4968448.
STRINGi10090.ENSMUSP00000034022.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi135 – 1384Combined sources
Beta strandi151 – 1533Combined sources
Helixi155 – 16410Combined sources
Helixi175 – 18612Combined sources
Helixi193 – 20614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LD7NMR-A130-220[»]
ProteinModelPortaliO88574.
SMRiO88574. Positions 64-220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88574.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 129129Interaction with NCOR1Add
BLAST
Regioni130 – 22091Interaction with SIN3AAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 5945Ala-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SAP30 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri67 – 11549AtypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG81414.
GeneTreeiENSGT00390000006633.
HOGENOMiHOG000007811.
HOVERGENiHBG057907.
InParanoidiO88574.
OMAiKIDSGVH.
OrthoDBiEOG7P5T35.
PhylomeDBiO88574.
TreeFamiTF324135.

Family and domain databases

InterProiIPR024145. His_deAcase_SAP30/SAP30L.
IPR025718. SAP30_Sin3-bd.
IPR025717. SAP30_zn-finger.
[Graphical view]
PANTHERiPTHR13286. PTHR13286. 1 hit.
PfamiPF13867. SAP30_Sin3_bdg. 1 hit.
PF13866. zf-SAP30. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88574-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGFTPEEMS RGGDAAAAVA AVVAAAAAAA SAGNGNAAGG GAEVPGAGAV
60 70 80 90 100
SASGPPGAAG PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI
110 120 130 140 150
ELDKSARHLY ICDYHKNLIQ SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL
160 170 180 190 200
YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFKSIP VNEKDTLTCF
210 220
IYSVRNDKNK SDLKADSGVH
Length:220
Mass (Da):23,231
Last modified:November 1, 1998 - v1
Checksum:iA9D4CBDAB97BBD8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075136 mRNA. Translation: AAC26007.1.
AK010928 mRNA. Translation: BAB27273.1.
AK088745 mRNA. Translation: BAC40543.1.
CH466569 Genomic DNA. Translation: EDL28616.1.
BC132081 mRNA. Translation: AAI32082.1.
BC132087 mRNA. Translation: AAI32088.1.
AJ251216 Genomic DNA. Translation: CAC24848.1.
CCDSiCCDS22316.1.
RefSeqiNP_068560.1. NM_021788.2.
UniGeneiMm.118.

Genome annotation databases

EnsembliENSMUST00000034022; ENSMUSP00000034022; ENSMUSG00000031609.
GeneIDi60406.
KEGGimmu:60406.
UCSCiuc009lsu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075136 mRNA. Translation: AAC26007.1.
AK010928 mRNA. Translation: BAB27273.1.
AK088745 mRNA. Translation: BAC40543.1.
CH466569 Genomic DNA. Translation: EDL28616.1.
BC132081 mRNA. Translation: AAI32082.1.
BC132087 mRNA. Translation: AAI32088.1.
AJ251216 Genomic DNA. Translation: CAC24848.1.
CCDSiCCDS22316.1.
RefSeqiNP_068560.1. NM_021788.2.
UniGeneiMm.118.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LD7NMR-A130-220[»]
ProteinModelPortaliO88574.
SMRiO88574. Positions 64-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208559. 4 interactions.
IntActiO88574. 7 interactions.
MINTiMINT-4968448.
STRINGi10090.ENSMUSP00000034022.

PTM databases

PhosphoSiteiO88574.

Proteomic databases

MaxQBiO88574.
PRIDEiO88574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034022; ENSMUSP00000034022; ENSMUSG00000031609.
GeneIDi60406.
KEGGimmu:60406.
UCSCiuc009lsu.2. mouse.

Organism-specific databases

CTDi8819.
MGIiMGI:1929129. Sap30.

Phylogenomic databases

eggNOGiNOG81414.
GeneTreeiENSGT00390000006633.
HOGENOMiHOG000007811.
HOVERGENiHBG057907.
InParanoidiO88574.
OMAiKIDSGVH.
OrthoDBiEOG7P5T35.
PhylomeDBiO88574.
TreeFamiTF324135.

Enzyme and pathway databases

ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_257106. HDACs deacetylate histones.

Miscellaneous databases

EvolutionaryTraceiO88574.
NextBioi314849.
PROiO88574.
SOURCEiSearch...

Gene expression databases

BgeeiO88574.
GenevestigatoriO88574.

Family and domain databases

InterProiIPR024145. His_deAcase_SAP30/SAP30L.
IPR025718. SAP30_Sin3-bd.
IPR025717. SAP30_zn-finger.
[Graphical view]
PANTHERiPTHR13286. PTHR13286. 1 hit.
PfamiPF13867. SAP30_Sin3_bdg. 1 hit.
PF13866. zf-SAP30. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors."
    Laherty C.D., Billin A.N., Lavinsky R.M., Yochum G.S., Bush A.C., Sun J.-M., Mullen T.-M., Davie J.R., Rose D.W., Glass C.K., Rosenfeld M.G., Ayer D.E., Eisenman R.N.
    Mol. Cell 2:33-42(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NCOR1; SIN3A; SIN3B; HDAC1; HDAC2 AND RBBP4.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Liver and Thymus.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Mouse scrapie responsive gene 1 (Scrg1): genomic organization, physical linkage to sap30, genetic mapping on chromosome 8, and expression in neuronal primary cell cultures."
    Dron M., Tartare X., Guillo F., Haik S., Barbin G., Maury C., Tovey M., Dandoy-Dron F.
    Genomics 70:140-149(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 181-220.
    Strain: C57BL/6NImported.
    Tissue: LiverImported.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: INTERACTION WITH SAMSN1.

Entry informationi

Entry nameiSAP30_MOUSE
AccessioniPrimary (citable) accession number: O88574
Secondary accession number(s): A2RSE9, Q99JB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: November 1, 1998
Last modified: February 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.