O88574 (SAP30_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone deacetylase complex subunit SAP30

Alternative name(s):
30 kDa Sin3-associated polypeptide
Sin3 corepressor complex subunit SAP30
Sin3-associated polypeptide p30

Gene names

Name:

Sap30

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. Ref.1 UniProtKB O75446
Subunit structure	Interacts with HCFC1 By similarity. A component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2. Interacts with SIN3A, SIN3B, HDAC1, HDAC2, RBBP4 and NCOR1. Interacts directly with SAMSN1. Ref.1 Ref.6 UniProtKB O75446
Subcellular location	Nucleus Ref.1.
Sequence similarities	Belongs to the SAP30 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ncor1	Q60974	3	EBI-593511,EBI-349004
Sin3a	Q60520	6	EBI-593511,EBI-349034

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

Histone deacetylase complex subunit SAP30

PRO_0000097583

Regions

Zinc finger

67 – 115

49

Atypical

Region

1 – 129

129

Interaction with NCOR1

Region

130 – 220

91

Interaction with SIN3A

Compositional bias

15 – 59

45

Ala-rich

Amino acid modifications

Modified residue

104

1

N6-acetyllysine By similarity

Modified residue

131

1

Phosphoserine By similarity

Modified residue

138

1

Phosphoserine By similarity

Modified residue

145

1

Phosphothreonine By similarity

Modified residue

169

1

Phosphothreonine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

220

Helix Strand Turn

Details...

Beta strand

151 – 153

3

Helix

155 – 164

10

Helix

175 – 181

7

Helix

186 – 188

3

Helix

193 – 206

14

Beta strand

208 – 210

3

Sequences

	Sequence		Length	Mass (Da)
	O88574 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A9D4CBDAB97BBD8C	FASTA	220	23,231
	10 20 30 40 50 60 MNGFTPEEMS RGGDAAAAVA AVVAAAAAAA SAGNGNAAGG GAEVPGAGAV SASGPPGAAG 70 80 90 100 110 120 PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI ELDKSARHLY ICDYHKNLIQ 130 140 150 160 170 180 SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE 190 200 210 220 IVGCHFKSIP VNEKDTLTCF IYSVRNDKNK SDLKADSGVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors." Laherty C.D., Billin A.N., Lavinsky R.M., Yochum G.S., Bush A.C., Sun J.-M., Mullen T.-M., Davie J.R., Rose D.W., Glass C.K., Rosenfeld M.G., Ayer D.E., Eisenman R.N. Mol. Cell 2:33-42(1998) [PubMed: 9702189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NCOR1; SIN3A; SIN3B; HDAC1; HDAC2 AND RBBP4.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Liver and Thymus.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Mouse scrapie responsive gene 1 (Scrg1): genomic organization, physical linkage to sap30, genetic mapping on chromosome 8, and expression in neuronal primary cell cultures." Dron M., Tartare X., Guillo F., Haik S., Barbin G., Maury C., Tovey M., Dandoy-Dron F. Genomics 70:140-149(2000) [PubMed: 11087671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 181-220. Strain: C57BL/6N. Tissue: Liver.
[6]	"SLy2 targets the nuclear SAP30/HDAC1 complex." Brandt S., Ellwanger K., Beuter-Gunia C., Schuster M., Hausser A., Schmitz I., Beer-Hammer S. Int. J. Biochem. Cell Biol. 42:1472-1481(2010) [PubMed: 20478393] [Abstract] Cited for: INTERACTION WITH SAMSN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF075136 mRNA. Translation: AAC26007.1.
AK010928 mRNA. Translation: BAB27273.1.
AK088745 mRNA. Translation: BAC40543.1.
CH466569 Genomic DNA. Translation: EDL28616.1.
BC132081 mRNA. Translation: AAI32082.1.
BC132087 mRNA. Translation: AAI32088.1.
AJ251216 Genomic DNA. Translation: CAC24848.1.

IPI

IPI00132028.

RefSeq

NP_068560.1. NM_021788.2.

UniGene

Mm.118.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LD7	NMR	-	A	130-220	[»]

ProteinModelPortal

O88574.

SMR

O88574. Positions 64-220.

ModBase

Search...

Protein-protein interaction databases

IntAct

O88574. 6 interactions.

MINT

MINT-4968448.

STRING

O88574.

PTM databases

PhosphoSite

O88574.

Proteomic databases

PRIDE

O88574.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000034022; ENSMUSP00000034022; ENSMUSG00000031609.

GeneID

60406.

KEGG

mmu:60406.

UCSC

uc009lsu.2. mouse.

Organism-specific databases

CTD

8819.

MGI

MGI:1929129. Sap30.

Phylogenomic databases

eggNOG

roNOG16658.

GeneTree

ENSGT00390000006633.

HOGENOM

HBG279868.

HOVERGEN

HBG057907.

InParanoid

O88574.

OMA

FTPDEMS.

OrthoDB

EOG48PMMK.

PhylomeDB

O88574.

Gene expression databases

ArrayExpress

O88574.

Bgee

O88574.

Genevestigator

O88574.

GermOnline

ENSMUSG00000031609. Mus musculus.

Family and domain databases

InterPro

IPR024145. His_deAcase_SAP30/SAP30L.
[Graphical view]

PANTHER

PTHR13286. PTHR13286. 1 hit.

ProtoNet

Search...

Other

NextBio

314849.

SOURCE

Search...

Entry information

Entry name

SAP30_MOUSE

Accession

Primary (citable) accession number: O88574
Secondary accession number(s): A2RSE9, Q99JB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program