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O88566

- AXIN2_MOUSE

UniProt

O88566 - AXIN2_MOUSE

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Protein

Axin-2

Gene

Axin2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity).By similarity

GO - Molecular functioni

  1. armadillo repeat domain binding Source: RefGenome
  2. beta-catenin binding Source: RefGenome
  3. GTPase activator activity Source: RefGenome
  4. I-SMAD binding Source: MGI
  5. protein kinase binding Source: RefGenome
  6. ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  1. bone mineralization Source: MGI
  2. cell death Source: RefGenome
  3. cell proliferation Source: MGI
  4. cellular protein localization Source: Ensembl
  5. cellular response to organic cyclic compound Source: RefGenome
  6. chondrocyte differentiation involved in endochondral bone morphogenesis Source: MGI
  7. dorsal/ventral axis specification Source: RefGenome
  8. intramembranous ossification Source: MGI
  9. maintenance of DNA repeat elements Source: Ensembl
  10. mRNA stabilization Source: Ensembl
  11. negative regulation of canonical Wnt signaling pathway Source: RefGenome
  12. negative regulation of catenin import into nucleus Source: Ensembl
  13. negative regulation of cell proliferation Source: MGI
  14. negative regulation of osteoblast differentiation Source: MGI
  15. odontogenesis Source: Ensembl
  16. positive regulation of cell death Source: Ensembl
  17. positive regulation of epithelial to mesenchymal transition Source: Ensembl
  18. positive regulation of GTPase activity Source: GOC
  19. positive regulation of protein phosphorylation Source: Ensembl
  20. regulation of catenin import into nucleus Source: RefGenome
  21. regulation of centromeric sister chromatid cohesion Source: BHF-UCL
  22. regulation of chondrocyte development Source: MGI
  23. regulation of mismatch repair Source: Ensembl
  24. regulation of transcription, DNA-templated Source: RefGenome
  25. regulation of Wnt signaling pathway Source: MGI
  26. secondary heart field specification Source: MGI
  27. somitogenesis Source: MGI
  28. termination of G-protein coupled receptor signaling pathway Source: InterPro
  29. Wnt signaling pathway involved in somitogenesis Source: RefGenome
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_207044. TCF dependent signaling in response to WNT.
REACT_219129. degradation of AXIN.

Names & Taxonomyi

Protein namesi
Recommended name:
Axin-2
Alternative name(s):
Axin-like protein
Short name:
Axil
Axis inhibition protein 2
Conductin
Gene namesi
Name:Axin2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1270862. Axin2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. beta-catenin destruction complex Source: RefGenome
  2. cell cortex Source: RefGenome
  3. centrosome Source: BHF-UCL
  4. cytoplasmic membrane-bounded vesicle Source: RefGenome
  5. cytoplasmic microtubule Source: RefGenome
  6. nucleus Source: MGI
  7. plasma membrane Source: RefGenome
  8. postsynaptic density Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840Axin-2PRO_0000220896Add
BLAST

Post-translational modificationi

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.By similarity
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription (By similarity).By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO88566.
PRIDEiO88566.

PTM databases

PhosphoSiteiO88566.

Expressioni

Gene expression databases

BgeeiO88566.
CleanExiMM_AXIN2.
ExpressionAtlasiO88566. baseline and differential.
GenevestigatoriO88566.

Interactioni

Subunit structurei

Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Interacts with SMAD7 and RNF111 (By similarity). Interacts with ANKRD6.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AMER3Q8N9442EBI-7690990,EBI-8869590From a different organism.
CDC20Q128342EBI-7690990,EBI-367462From a different organism.
CEP250Q9BV733EBI-7690990,EBI-1053100From a different organism.

Protein-protein interaction databases

BioGridi198288. 6 interactions.
IntActiO88566. 5 interactions.
MINTiMINT-1951339.

Structurei

3D structure databases

ProteinModelPortaliO88566.
SMRiO88566. Positions 74-199, 761-840.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 200120RGSPROSITE-ProRule annotationAdd
BLAST
Domaini758 – 84083DIXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 41387Interaction with GSK3BBy similarityAdd
BLAST
Regioni413 – 47866Interaction with beta-cateninBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3010Tankyrase-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi469 – 4768Poly-His

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

Sequence similaritiesi

Contains 1 DIX domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG238205.
GeneTreeiENSGT00390000010011.
HOGENOMiHOG000231228.
HOVERGENiHBG004324.
InParanoidiO88566.
KOiK04385.
OMAiKHVHHHY.
OrthoDBiEOG79PJQ0.
TreeFamiTF315454.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88566-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP
60 70 80 90 100
VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE
110 120 130 140 150
REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK
160 170 180 190 200
PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV
210 220 230 240 250
RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL
260 270 280 290 300
SSKTLRATAS VRSTETAENG FRSFKRSDPV NPYHVGSGYV FAPATSANDS
310 320 330 340 350
ELSSDALTDD SMSMTDSSVD GVPPYRMGSK KQLQREMHRS VKANGQVSLP
360 370 380 390 400
HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE
410 420 430 440 450
DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP
460 470 480 490 500
GCQSPGVGRY SPRSRSPDHH HQHHHHQQCH TLLPTGGKLP PVAACPLLGG
510 520 530 540 550
KSFLTKQTTK HVHHHYIHHH AVPKTKEEIE AEATQRVRCL CPGGTDYYCY
560 570 580 590 600
SKCKSHPKAP EPLPGEQFCG SRGGTLPKRN AKGTEPGLAL SARDGGMSSA
610 620 630 640 650
AGAPQLPGEE GDRSQDVWQW MLESERQSKS KPHSAQSIRK SYPLESACAA
660 670 680 690 700
PGERVSRHHL LGASGHSRSV ARAHPFTQDP AMPPLTPPNT LAQLEEACRR
710 720 730 740 750
LAEVSKPQKQ RCCVASQQRD RNHSAAGQAG ASPFANPSLA PEDHKEPKKL
760 770 780 790 800
ASVHALQASE LVVTYFFCGE EIPYRRMLKA QSLTLGHFKE QLSKKGNYRY
810 820 830 840
YFKKASDEFA CGAVFEEIWD DETVLPMYEG RILGKVERID
Length:840
Mass (Da):92,906
Last modified:July 27, 2011 - v2
Checksum:i53D7D33128879298
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011R → K in AAF22800. 1 PublicationCurated
Sequence conflicti474 – 4741H → Y in AAF22800. 1 PublicationCurated
Sequence conflicti484 – 4841P → S in AAC26047. (PubMed:9554852)Curated
Sequence conflicti503 – 5031F → S in AAF22800. 1 PublicationCurated
Sequence conflicti603 – 6031A → G in AAC26047. (PubMed:9554852)Curated
Sequence conflicti648 – 6481C → R in AAC26047. (PubMed:9554852)Curated
Sequence conflicti648 – 6481C → R in AAF22800. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF073788 mRNA. Translation: AAC26047.1.
AF205889 mRNA. Translation: AAF22800.1.
AL662893 Genomic DNA. Translation: CAM17369.1.
CH466558 Genomic DNA. Translation: EDL34346.1.
BC057338 mRNA. Translation: AAH57338.1.
CCDSiCCDS25575.1.
RefSeqiNP_056547.3. NM_015732.4.
XP_006532120.1. XM_006532057.1.
XP_006532121.1. XM_006532058.1.
XP_006532122.1. XM_006532059.1.
XP_006532123.1. XM_006532060.1.
UniGeneiMm.71710.

Genome annotation databases

EnsembliENSMUST00000052915; ENSMUSP00000051331; ENSMUSG00000000142.
GeneIDi12006.
KEGGimmu:12006.
UCSCiuc007mbu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF073788 mRNA. Translation: AAC26047.1 .
AF205889 mRNA. Translation: AAF22800.1 .
AL662893 Genomic DNA. Translation: CAM17369.1 .
CH466558 Genomic DNA. Translation: EDL34346.1 .
BC057338 mRNA. Translation: AAH57338.1 .
CCDSi CCDS25575.1.
RefSeqi NP_056547.3. NM_015732.4.
XP_006532120.1. XM_006532057.1.
XP_006532121.1. XM_006532058.1.
XP_006532122.1. XM_006532059.1.
XP_006532123.1. XM_006532060.1.
UniGenei Mm.71710.

3D structure databases

ProteinModelPortali O88566.
SMRi O88566. Positions 74-199, 761-840.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198288. 6 interactions.
IntActi O88566. 5 interactions.
MINTi MINT-1951339.

Chemistry

ChEMBLi CHEMBL1255128.

PTM databases

PhosphoSitei O88566.

Proteomic databases

MaxQBi O88566.
PRIDEi O88566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052915 ; ENSMUSP00000051331 ; ENSMUSG00000000142 .
GeneIDi 12006.
KEGGi mmu:12006.
UCSCi uc007mbu.2. mouse.

Organism-specific databases

CTDi 8313.
MGIi MGI:1270862. Axin2.

Phylogenomic databases

eggNOGi NOG238205.
GeneTreei ENSGT00390000010011.
HOGENOMi HOG000231228.
HOVERGENi HBG004324.
InParanoidi O88566.
KOi K04385.
OMAi KHVHHHY.
OrthoDBi EOG79PJQ0.
TreeFami TF315454.

Enzyme and pathway databases

Reactomei REACT_207044. TCF dependent signaling in response to WNT.
REACT_219129. degradation of AXIN.

Miscellaneous databases

NextBioi 280199.
PROi O88566.
SOURCEi Search...

Gene expression databases

Bgeei O88566.
CleanExi MM_AXIN2.
ExpressionAtlasi O88566. baseline and differential.
Genevestigatori O88566.

Family and domain databases

Gene3Di 1.10.196.10. 2 hits.
InterProi IPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR01301. RGSPROTEIN.
SMARTi SM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta."
    Behrens J., Jerchow B.-A., Wuertele M., Grimm J., Asbrand C., Wirtz R., Kuehl M., Wedlich D., Birchmeier W.
    Science 280:596-599(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Properties of mouse Axin2 and human AXIN2: chromosomal location, expression pattern, interaction with Axin and effects on embryonic axis formation."
    Zhang T., Fagotto F., Hsu W., Zeng L., Gilbert D., Copeland N.G., Jenkins N.A., Warburton D., Costantini F.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. "The ankyrin repeat protein diversin recruits casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling."
    Schwarz-Romond T., Asbrand C., Bakkers J., Kuehl M., Schaeffer H.J., Huelsken J., Behrens J., Hammerschmidt M., Birchmeier W.
    Genes Dev. 16:2073-2084(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ANKRD6.

Entry informationi

Entry nameiAXIN2_MOUSE
AccessioniPrimary (citable) accession number: O88566
Secondary accession number(s): Q6PFZ9, Q9QXJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3