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O88566

- AXIN2_MOUSE

UniProt

O88566 - AXIN2_MOUSE

Protein

Axin-2

Gene

Axin2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B By similarity.By similarity

    GO - Molecular functioni

    1. armadillo repeat domain binding Source: RefGenome
    2. beta-catenin binding Source: RefGenome
    3. GTPase activator activity Source: RefGenome
    4. I-SMAD binding Source: MGI
    5. protein binding Source: IntAct
    6. protein kinase binding Source: RefGenome
    7. signal transducer activity Source: InterPro
    8. ubiquitin protein ligase binding Source: MGI

    GO - Biological processi

    1. bone mineralization Source: MGI
    2. cell death Source: RefGenome
    3. cell proliferation Source: MGI
    4. cellular protein localization Source: Ensembl
    5. cellular response to organic cyclic compound Source: RefGenome
    6. chondrocyte differentiation involved in endochondral bone morphogenesis Source: MGI
    7. dorsal/ventral axis specification Source: RefGenome
    8. intramembranous ossification Source: MGI
    9. maintenance of DNA repeat elements Source: Ensembl
    10. mRNA stabilization Source: Ensembl
    11. negative regulation of canonical Wnt signaling pathway Source: RefGenome
    12. negative regulation of catenin import into nucleus Source: Ensembl
    13. negative regulation of cell proliferation Source: MGI
    14. negative regulation of osteoblast differentiation Source: MGI
    15. odontogenesis Source: Ensembl
    16. positive regulation of cell death Source: Ensembl
    17. positive regulation of epithelial to mesenchymal transition Source: Ensembl
    18. positive regulation of GTPase activity Source: GOC
    19. positive regulation of protein phosphorylation Source: Ensembl
    20. regulation of catenin import into nucleus Source: RefGenome
    21. regulation of centromeric sister chromatid cohesion Source: BHF-UCL
    22. regulation of chondrocyte development Source: MGI
    23. regulation of mismatch repair Source: Ensembl
    24. regulation of transcription, DNA-templated Source: RefGenome
    25. regulation of Wnt signaling pathway Source: MGI
    26. secondary heart field specification Source: MGI
    27. somitogenesis Source: MGI
    28. termination of G-protein coupled receptor signaling pathway Source: InterPro
    29. Wnt signaling pathway involved in somitogenesis Source: RefGenome

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_207044. TCF dependent signaling in response to WNT.
    REACT_219129. degradation of AXIN.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Axin-2
    Alternative name(s):
    Axin-like protein
    Short name:
    Axil
    Axis inhibition protein 2
    Conductin
    Gene namesi
    Name:Axin2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1270862. Axin2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. beta-catenin destruction complex Source: RefGenome
    2. cell cortex Source: RefGenome
    3. centrosome Source: BHF-UCL
    4. cytoplasmic membrane-bounded vesicle Source: RefGenome
    5. cytoplasmic microtubule Source: RefGenome
    6. nucleus Source: MGI
    7. plasma membrane Source: RefGenome
    8. postsynaptic density Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 840840Axin-2PRO_0000220896Add
    BLAST

    Post-translational modificationi

    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.By similarity
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway By similarity.By similarity
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription By similarity.By similarity

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiO88566.

    PTM databases

    PhosphoSiteiO88566.

    Expressioni

    Gene expression databases

    ArrayExpressiO88566.
    BgeeiO88566.
    CleanExiMM_AXIN2.
    GenevestigatoriO88566.

    Interactioni

    Subunit structurei

    Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Interacts with SMAD7 and RNF111 By similarity. Interacts with ANKRD6.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AMER3Q8N9442EBI-7690990,EBI-8869590From a different organism.
    CDC20Q128342EBI-7690990,EBI-367462From a different organism.
    CEP250Q9BV733EBI-7690990,EBI-1053100From a different organism.

    Protein-protein interaction databases

    BioGridi198288. 6 interactions.
    IntActiO88566. 5 interactions.
    MINTiMINT-1951339.

    Structurei

    3D structure databases

    ProteinModelPortaliO88566.
    SMRiO88566. Positions 74-199, 761-840.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 200120RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini758 – 84083DIXPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni327 – 41387Interaction with GSK3BBy similarityAdd
    BLAST
    Regioni413 – 47866Interaction with beta-cateninBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi21 – 3010Tankyrase-binding motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi469 – 4768Poly-His

    Domaini

    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

    Sequence similaritiesi

    Contains 1 DIX domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238205.
    GeneTreeiENSGT00390000010011.
    HOGENOMiHOG000231228.
    HOVERGENiHBG004324.
    InParanoidiQ6PFZ9.
    KOiK04385.
    OMAiKHVHHHY.
    OrthoDBiEOG79PJQ0.
    TreeFamiTF315454.

    Family and domain databases

    Gene3Di1.10.196.10. 2 hits.
    InterProiIPR014936. Axin_b-cat-bd.
    IPR001158. DIX.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF08833. Axin_b-cat_bind. 1 hit.
    PF00778. DIX. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR01301. RGSPROTEIN.
    SMARTiSM00021. DAX. 1 hit.
    SM00315. RGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50841. DIX. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88566-1 [UniParc]FASTAAdd to Basket

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    MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP    50
    VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE 100
    REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK 150
    PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV 200
    RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL 250
    SSKTLRATAS VRSTETAENG FRSFKRSDPV NPYHVGSGYV FAPATSANDS 300
    ELSSDALTDD SMSMTDSSVD GVPPYRMGSK KQLQREMHRS VKANGQVSLP 350
    HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE 400
    DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP 450
    GCQSPGVGRY SPRSRSPDHH HQHHHHQQCH TLLPTGGKLP PVAACPLLGG 500
    KSFLTKQTTK HVHHHYIHHH AVPKTKEEIE AEATQRVRCL CPGGTDYYCY 550
    SKCKSHPKAP EPLPGEQFCG SRGGTLPKRN AKGTEPGLAL SARDGGMSSA 600
    AGAPQLPGEE GDRSQDVWQW MLESERQSKS KPHSAQSIRK SYPLESACAA 650
    PGERVSRHHL LGASGHSRSV ARAHPFTQDP AMPPLTPPNT LAQLEEACRR 700
    LAEVSKPQKQ RCCVASQQRD RNHSAAGQAG ASPFANPSLA PEDHKEPKKL 750
    ASVHALQASE LVVTYFFCGE EIPYRRMLKA QSLTLGHFKE QLSKKGNYRY 800
    YFKKASDEFA CGAVFEEIWD DETVLPMYEG RILGKVERID 840
    Length:840
    Mass (Da):92,906
    Last modified:July 27, 2011 - v2
    Checksum:i53D7D33128879298
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011R → K in AAF22800. 1 PublicationCurated
    Sequence conflicti474 – 4741H → Y in AAF22800. 1 PublicationCurated
    Sequence conflicti484 – 4841P → S in AAC26047. (PubMed:9554852)Curated
    Sequence conflicti503 – 5031F → S in AAF22800. 1 PublicationCurated
    Sequence conflicti603 – 6031A → G in AAC26047. (PubMed:9554852)Curated
    Sequence conflicti648 – 6481C → R in AAC26047. (PubMed:9554852)Curated
    Sequence conflicti648 – 6481C → R in AAF22800. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073788 mRNA. Translation: AAC26047.1.
    AF205889 mRNA. Translation: AAF22800.1.
    AL662893 Genomic DNA. Translation: CAM17369.1.
    CH466558 Genomic DNA. Translation: EDL34346.1.
    BC057338 mRNA. Translation: AAH57338.1.
    CCDSiCCDS25575.1.
    RefSeqiNP_056547.3. NM_015732.4.
    XP_006532120.1. XM_006532057.1.
    XP_006532121.1. XM_006532058.1.
    XP_006532122.1. XM_006532059.1.
    XP_006532123.1. XM_006532060.1.
    UniGeneiMm.71710.

    Genome annotation databases

    EnsembliENSMUST00000052915; ENSMUSP00000051331; ENSMUSG00000000142.
    GeneIDi12006.
    KEGGimmu:12006.
    UCSCiuc007mbu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF073788 mRNA. Translation: AAC26047.1 .
    AF205889 mRNA. Translation: AAF22800.1 .
    AL662893 Genomic DNA. Translation: CAM17369.1 .
    CH466558 Genomic DNA. Translation: EDL34346.1 .
    BC057338 mRNA. Translation: AAH57338.1 .
    CCDSi CCDS25575.1.
    RefSeqi NP_056547.3. NM_015732.4.
    XP_006532120.1. XM_006532057.1.
    XP_006532121.1. XM_006532058.1.
    XP_006532122.1. XM_006532059.1.
    XP_006532123.1. XM_006532060.1.
    UniGenei Mm.71710.

    3D structure databases

    ProteinModelPortali O88566.
    SMRi O88566. Positions 74-199, 761-840.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198288. 6 interactions.
    IntActi O88566. 5 interactions.
    MINTi MINT-1951339.

    Chemistry

    ChEMBLi CHEMBL1255128.

    PTM databases

    PhosphoSitei O88566.

    Proteomic databases

    PRIDEi O88566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052915 ; ENSMUSP00000051331 ; ENSMUSG00000000142 .
    GeneIDi 12006.
    KEGGi mmu:12006.
    UCSCi uc007mbu.2. mouse.

    Organism-specific databases

    CTDi 8313.
    MGIi MGI:1270862. Axin2.

    Phylogenomic databases

    eggNOGi NOG238205.
    GeneTreei ENSGT00390000010011.
    HOGENOMi HOG000231228.
    HOVERGENi HBG004324.
    InParanoidi Q6PFZ9.
    KOi K04385.
    OMAi KHVHHHY.
    OrthoDBi EOG79PJQ0.
    TreeFami TF315454.

    Enzyme and pathway databases

    Reactomei REACT_207044. TCF dependent signaling in response to WNT.
    REACT_219129. degradation of AXIN.

    Miscellaneous databases

    NextBioi 280199.
    PROi O88566.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88566.
    Bgeei O88566.
    CleanExi MM_AXIN2.
    Genevestigatori O88566.

    Family and domain databases

    Gene3Di 1.10.196.10. 2 hits.
    InterProi IPR014936. Axin_b-cat-bd.
    IPR001158. DIX.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF08833. Axin_b-cat_bind. 1 hit.
    PF00778. DIX. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR01301. RGSPROTEIN.
    SMARTi SM00021. DAX. 1 hit.
    SM00315. RGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50841. DIX. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta."
      Behrens J., Jerchow B.-A., Wuertele M., Grimm J., Asbrand C., Wirtz R., Kuehl M., Wedlich D., Birchmeier W.
      Science 280:596-599(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Properties of mouse Axin2 and human AXIN2: chromosomal location, expression pattern, interaction with Axin and effects on embryonic axis formation."
      Zhang T., Fagotto F., Hsu W., Zeng L., Gilbert D., Copeland N.G., Jenkins N.A., Warburton D., Costantini F.
      Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    6. "The ankyrin repeat protein diversin recruits casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling."
      Schwarz-Romond T., Asbrand C., Bakkers J., Kuehl M., Schaeffer H.J., Huelsken J., Behrens J., Hammerschmidt M., Birchmeier W.
      Genes Dev. 16:2073-2084(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ANKRD6.

    Entry informationi

    Entry nameiAXIN2_MOUSE
    AccessioniPrimary (citable) accession number: O88566
    Secondary accession number(s): Q6PFZ9, Q9QXJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3