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Protein

Canalicular multispecific organic anion transporter 2

Gene

Abcc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi659 – 6668ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1318 – 13258ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: MGI
  • ATP binding Source: UniProtKB-KW
  • bile acid-exporting ATPase activity Source: RGD
  • drug transmembrane transporter activity Source: RGD
  • xenobiotic-transporting ATPase activity Source: RGD

GO - Biological processi

  • canalicular bile acid transport Source: MGI
  • drug transmembrane transport Source: RGD
  • drug transport Source: RGD
  • response to estradiol Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to organonitrogen compound Source: RGD
  • xenobiotic transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Canalicular multispecific organic anion transporter 2
Alternative name(s):
ATP-binding cassette sub-family C member 3
MRP-like protein 2
Short name:
MLP-2
Multidrug resistance-associated protein 3
Gene namesi
Name:Abcc3
Synonyms:Cmoat2, Mlp2, Mrp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71101. Abcc3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularBy similarityAdd
BLAST
Transmembranei33 – 5321Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini54 – 7320CytoplasmicBy similarityAdd
BLAST
Transmembranei74 – 9421Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini95 – 995ExtracellularBy similarity
Transmembranei100 – 12021Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini121 – 13212CytoplasmicBy similarityAdd
BLAST
Transmembranei133 – 15321Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini154 – 17118ExtracellularBy similarityAdd
BLAST
Transmembranei172 – 19221Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini193 – 301109CytoplasmicBy similarityAdd
BLAST
Transmembranei302 – 32221Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini323 – 34725ExtracellularBy similarityAdd
BLAST
Transmembranei348 – 36821Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini369 – 42456CytoplasmicBy similarityAdd
BLAST
Transmembranei425 – 44521Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini446 – 4483ExtracellularBy similarity
Transmembranei449 – 46921Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini470 – 53162CytoplasmicBy similarityAdd
BLAST
Transmembranei532 – 55221Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Topological domaini553 – 57422ExtracellularBy similarityAdd
BLAST
Transmembranei575 – 59521Helical; Name=11PROSITE-ProRule annotationAdd
BLAST
Topological domaini596 – 958363CytoplasmicBy similarityAdd
BLAST
Transmembranei959 – 97921Helical; Name=12PROSITE-ProRule annotationAdd
BLAST
Topological domaini980 – 101637ExtracellularBy similarityAdd
BLAST
Transmembranei1017 – 103721Helical; Name=13PROSITE-ProRule annotationAdd
BLAST
Topological domaini1038 – 108043CytoplasmicBy similarityAdd
BLAST
Transmembranei1081 – 110121Helical; Name=14PROSITE-ProRule annotationAdd
BLAST
Topological domaini1102 – 11021ExtracellularBy similarity
Transmembranei1103 – 112321Helical; Name=15PROSITE-ProRule annotationAdd
BLAST
Topological domaini1124 – 119471CytoplasmicBy similarityAdd
BLAST
Transmembranei1195 – 121521Helical; Name=16PROSITE-ProRule annotationAdd
BLAST
Topological domaini1216 – 12172ExtracellularBy similarity
Transmembranei1218 – 123821Helical; Name=17PROSITE-ProRule annotationAdd
BLAST
Topological domaini1239 – 1522284CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • integral component of plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2073682.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15221522Canalicular multispecific organic anion transporter 2PRO_0000093361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi18 – 181N-linked (GlcNAc...)Sequence analysis
Modified residuei902 – 9021PhosphoserineCombined sources
Modified residuei905 – 9051PhosphoserineCombined sources
Glycosylationi1001 – 10011N-linked (GlcNAc...)Sequence analysis
Glycosylationi1002 – 10021N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO88563.

PTM databases

iPTMnetiO88563.

Expressioni

Tissue specificityi

Lung and intestine, low in liver. Higher in liver of Eisai hyperbilirubinemic rats and TR- mutant rats.

Interactioni

Protein-protein interaction databases

BioGridi250828. 1 interaction.

Chemistry

BindingDBiO88563.

Structurei

3D structure databases

ProteinModelPortaliO88563.
SMRiO88563. Positions 624-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini310 – 592283ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini625 – 849225ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini966 – 1247282ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1286 – 1518233ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG108314.
InParanoidiO88563.
KOiK05667.
PhylomeDBiO88563.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030242. ABCC3.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF221. PTHR24223:SF221. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRLCGSGEL GSKFWDSNLT VYTNTPDLTP CFQNSLLAWV PCIYLWAALP
60 70 80 90 100
CYLFYLRHHR LGYIVLSCLS RLKTALGVLL WCISWVDLFY SFHGLVHGSS
110 120 130 140 150
PAPVFFITPL LVGITMLLAT LLIQYERLRG VRSSGVLIIF WLLCVICAII
160 170 180 190 200
PFRSKILLAL AEGKILDPFR FTTFYIYFAL VLCAFILSCF QEKPPLFSPE
210 220 230 240 250
NLDTNPCPEA SAGFFSRLSF WWFTKLAILG YRRPLEDSDL WSLSEEDCSH
260 270 280 290 300
KVVQRLLEAW QKQQTQASGP QTAALEPKIA GEDEVLLKAR PKTKKPSFLR
310 320 330 340 350
ALVRTFTSSL LMGACFKLIQ DLSPSSTHSC SASSSGLFRP HGPYWWGFLL
360 370 380 390 400
AGLMFVSSTM QTLILHQHYH CIFVMALRIR TAIIGVIYRK ALTITNSVKR
410 420 430 440 450
EYTVGEMVNL MSVDAQRFMD VSPFINLLWS APLQVILAIY FLWQILGPSA
460 470 480 490 500
LAGVAVIVLL IPLNGAVSMK MKTYQVQQMK FKDSRIKLMS EILNGIKVLK
510 520 530 540 550
LYAWEPTFLE QVEGIRQGEL QLLRKGAYLQ AISTFIWVCT PFMVTLITLG
560 570 580 590 600
VYVCVDKNNV LDAEKAFVSL SLFNILKIPL NLLPQLISGM TQTSVSLKRI
610 620 630 640 650
QDFLNQDELD PQCVERKTIS PGRAITIHNG TFSWSKDLPP TLHSINIQIP
660 670 680 690 700
KGALVAVVGP VGCGKSSLVS ALLGEMEKLE GAVSVKGSVA YVPQQAWIQN
710 720 730 740 750
CTLQENVLFG QPMNPKRYQQ ALETCALLAD LDVLPGGDQT EIGEKGINLS
760 770 780 790 800
GGQRQRVSLA RAVYSDANIF LLDDPLSAVD SHVAKHIFDQ VIGPEGVLAG
810 820 830 840 850
KTRVLVTHGI SFLPQTDFII VLADGQITEM GHYSELLQHD GSFANFLRNY
860 870 880 890 900
APDENQEANE GVLQHANEEV LLLEDTLSTH TDLTDTEPAI YEVRKQFMRE
910 920 930 940 950
MSSLSSEGEG QNRPVLKRYT SSLEKEVPAT QTKETGALIK EEIAETGNVK
960 970 980 990 1000
LSVYWDYAKS VGLCTTLFIC LLYAGQNAVA IGANVWLSAW TNDVEEHGQQ
1010 1020 1030 1040 1050
NNTSVRLGVY ATLGILQGLL VMLSAFTMVV GAIQAARLLH TALLHNQIRA
1060 1070 1080 1090 1100
PQSFFDTTPS GRILNRFSKD IYVIHEVLAP TILMLFNSFY TSISTIVVIV
1110 1120 1130 1140 1150
ASTPLFCVVV LPLAVFYGFV QRFYVATSRQ LKRLESVSRS PIFSHFSETV
1160 1170 1180 1190 1200
TGTSVIRAYG RVQDFKVLSD AKVDSNQKTT YPYIASNRWL GVHVEFVGNC
1210 1220 1230 1240 1250
VVLFSALFAV IGRNSLNPGL VGLSVSYALQ VTLSLNWMIR TLSDLESNII
1260 1270 1280 1290 1300
AVERVKEYSK TETEAPWVLE SNRAPEGWPR SGVVEFRNYS VRYRPGLELV
1310 1320 1330 1340 1350
LKNLTLHVQG GEKVGIVGRT GAGKSSMTLC LFRILEAAEG EIFIDGLNVA
1360 1370 1380 1390 1400
HIGLHDLRSQ LTIIPQDPIL FSGTLRMNLD PFGRYSDEDI WRTLELSHLS
1410 1420 1430 1440 1450
AFVSSQPTGL DFQCSEGGDN LSVGQRQLVC LARALLRKSR VLVLDEATAA
1460 1470 1480 1490 1500
IDLETDDLIQ GTIRTQFEDC TVLTIAHRLN TIMDYNRVLV LDKGVVAEFD
1510 1520
SPVNLIAAGG IFYGMAKDAG LA
Length:1,522
Mass (Da):168,978
Last modified:November 1, 1998 - v1
Checksum:i740E31E0C4C64297
GO

Sequence cautioni

The sequence BAA28955.1 differs from that shown. Reason: Frameshift at positions 323, 339 and 344. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti645 – 6451I → L in BAA28955 (PubMed:9614210).Curated
Sequence conflicti1075 – 10751H → D in BAA28955 (PubMed:9614210).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072816 mRNA. Translation: AAC25416.1.
AB010467 mRNA. Translation: BAA28955.1. Frameshift.
RefSeqiNP_542148.1. NM_080581.1.
UniGeneiRn.88248.

Genome annotation databases

GeneIDi140668.
KEGGirno:140668.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072816 mRNA. Translation: AAC25416.1.
AB010467 mRNA. Translation: BAA28955.1. Frameshift.
RefSeqiNP_542148.1. NM_080581.1.
UniGeneiRn.88248.

3D structure databases

ProteinModelPortaliO88563.
SMRiO88563. Positions 624-851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250828. 1 interaction.

Chemistry

BindingDBiO88563.
ChEMBLiCHEMBL2073682.

PTM databases

iPTMnetiO88563.

Proteomic databases

PRIDEiO88563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140668.
KEGGirno:140668.

Organism-specific databases

CTDi8714.
RGDi71101. Abcc3.

Phylogenomic databases

HOVERGENiHBG108314.
InParanoidiO88563.
KOiK05667.
PhylomeDBiO88563.

Miscellaneous databases

PROiO88563.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030242. ABCC3.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF221. PTHR24223:SF221. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MRP3, a new ATP-binding cassette protein localized to the canalicular domain of the hepatocyte."
    Ortiz D.F., Li S., Iyer R., Zhang X., Novikoff P., Arias I.M.
    Am. J. Physiol. 276:G1493-G1500(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Hepatic expression of multidrug resistance-associated protein-like proteins maintained in eisai hyperbilirubinemic rats."
    Hirohashi T., Suzuki H., Ito K., Ogawa K., Kume K., Shimizu T., Sugiyama Y.
    Mol. Pharmacol. 53:1068-1075(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Colon.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-902 AND SER-905, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMRP3_RAT
AccessioniPrimary (citable) accession number: O88563
Secondary accession number(s): O88270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.