O88561 (S27A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain fatty acid transport protein 3
Short name=FATP-3
Short name=Fatty acid transport protein 3
EC=6.2.1.-

Alternative name(s):
Solute carrier family 27 member 3

Gene names

Name:	Slc27a3
Synonyms:	Acsvl3, Fatp3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Does not exhibit fatty acid transport activity. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Potential. Mitochondrion. Note: Mitochondrial in MA-10 Leydig cell line and Neuro-2a neuroblastoma. Ref.4
Tissue specificity	Expressed at high levels in adrenal gland, testis and ovary. Expressed at lower levels in adult brain. Found in adrenal cortical cells, spermatocytes and interstitial cells of the testis, theca cells of the ovary, cerebral cortical neurons, and cerebellar Purkinje cells (at protein level). Ref.4
Developmental stage	Expressed at higher levels in embryonic brain (embryonic days 12-14) than in newborn or adult. Ref.4
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAH60052.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC36120.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acyl-CoA metabolic process Inferred by curator Ref.4. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay Ref.4. Source: MGI
Molecular_function	fatty-acyl-CoA synthase activity Inferred from mutant phenotype Ref.4. Source: MGI long-chain fatty acid-CoA ligase activity Traceable author statement PubMed 15464426. Source: MGI nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O88561-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O88561-2)

The sequence of this isoform differs from the canonical sequence as follows:
     437-528: HIFPFSLIRY...QGFLHFHDRT → VRDRVDRRTP...VGAEPRGSGK
     529-667: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Long-chain fatty acid transport protein 3		PRO_0000193208
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	315 – 335	21	Helical; Potential
	Nucleotide binding	269 – 280	12	AMP Potential
Natural variations
	Alternative sequence	437 – 528	92	HIFPF…FHDRT → VRDRVDRRTPGAEGGMARAG APGANDAVWLSLPAHLPLLL DSIRCHDRGAYSECPGALHD HISRFVLGGVQSWQTRLNLA PEVGAEPRGSGK in isoform 2.		VSP_016219
	Alternative sequence	529 – 667	139	Missing in isoform 2.		VSP_016220
Experimental info
	Sequence conflict	302	1	Missing in AAH60052. Ref.1
	Sequence conflict	376	1	C → F in AAC40187. Ref.2
	Sequence conflict	532	1	I → F Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: 0F343D14952075E7	FASTA	667	72,965
	10 20 30 40 50 60 MAALLLLLPL LLLLPLLLKL DVWPQLRWLP ADLAFTVRAL RCKRALRARA LAAAAADPES 70 80 90 100 110 120 SESGCSLAWR LAYLAREQPT HTFLIHGAQR FSYAEAERES NRIARAFLRA RGWTGGRRGS 130 140 150 160 170 180 GRGSTEEGAR VAPPAGDAAA RGTTAPPLAP GATVALLLPA GPDFLWIWFG LAKAGLRTAF 190 200 210 220 230 240 VPTALRRGPL LHCLRSCGAS ALVLATEFLE SLEPDLPALR AMGLHLWATG PETNVAGISN 250 260 270 280 290 300 LLSEAADQVD EPVPGYLSAP QNIMDTCLYI FTSGTTGLPK AARISHLKVL QCQGFYHLCG 310 320 330 340 350 360 VHQEDVIYLA LPLYHMSGSL LGIVGCLGIG ATVVLKPKFS ASQFWDDCQK HRVTVFQYIG 370 380 390 400 410 420 ELCRYLVNQP PSKAECDHKV RLAVGSGLRP DTWERFLRRF GPLQILETYG MTEGNVATFN 430 440 450 460 470 480 YTGRQGAVGR ASWLYKHIFP FSLIRYDVMT GEPIRNAQGH CMTTSPGEPG LLVAPVSQQS 490 500 510 520 530 540 PFLGYAGAPE LAKDKLLKDV FWSGDVFFNT GDLLVCDEQG FLHFHDRTGD TIRWKGENVA 550 560 570 580 590 600 TTEVAEVLET LDFLQEVNIY GVTVPGHEGR AGMAALALRP PQALNLVQLY SHVSENLPPY 610 620 630 640 650 660 ARPRFLRLQE SLATTETFKQ QKVRMANEGF DPSVLSDPLY VLDQDIGAYL PLTPARYSAL LSGDLRI
	Isoform 2 [UniParc]. Checksum: 910A0B14CC41CFB3	FASTA	528	57,181
	10 20 30 40 50 60 MAALLLLLPL LLLLPLLLKL DVWPQLRWLP ADLAFTVRAL RCKRALRARA LAAAAADPES 70 80 90 100 110 120 SESGCSLAWR LAYLAREQPT HTFLIHGAQR FSYAEAERES NRIARAFLRA RGWTGGRRGS 130 140 150 160 170 180 GRGSTEEGAR VAPPAGDAAA RGTTAPPLAP GATVALLLPA GPDFLWIWFG LAKAGLRTAF 190 200 210 220 230 240 VPTALRRGPL LHCLRSCGAS ALVLATEFLE SLEPDLPALR AMGLHLWATG PETNVAGISN 250 260 270 280 290 300 LLSEAADQVD EPVPGYLSAP QNIMDTCLYI FTSGTTGLPK AARISHLKVL QCQGFYHLCG 310 320 330 340 350 360 VHQEDVIYLA LPLYHMSGSL LGIVGCLGIG ATVVLKPKFS ASQFWDDCQK HRVTVFQYIG 370 380 390 400 410 420 ELCRYLVNQP PSKAECDHKV RLAVGSGLRP DTWERFLRRF GPLQILETYG MTEGNVATFN 430 440 450 460 470 480 YTGRQGAVGR ASWLYKVRDR VDRRTPGAEG GMARAGAPGA NDAVWLSLPA HLPLLLDSIR 490 500 510 520 CHDRGAYSEC PGALHDHISR FVLGGVQSWQ TRLNLAPEVG AEPRGSGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 45-667 (ISOFORM 1). Strain: C57BL/6J.
[3]	"A family of fatty acid transporters conserved from mycobacterium to man." Hirsch D., Stahl A., Lodish H.F. Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-667 (ISOFORM 1).
[4]	"Mouse very long-chain acyl-CoA synthetase 3/fatty acid transport protein 3 catalyzes fatty acid activation but not fatty acid transport in MA-10 cells." Pei Z., Fraisl P., Berger J., Jia Z., Forss-Petter S., Watkins P.A. J. Biol. Chem. 279:54454-54462(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC060052 mRNA. Translation: AAH60052.1. Different initiation. AK076014 mRNA. Translation: BAC36120.1. Different initiation. AF072758 mRNA. Translation: AAC40187.1.
IPI	IPI00131976. IPI00461239.
RefSeq	NP_036118.2. NM_011988.2.
UniGene	Mm.45519.
3D structure databases
ProteinModelPortal	O88561.
SMR	O88561. Positions 148-628.
ModBase	Search...
PTM databases
PhosphoSite	O88561.
Proteomic databases
PaxDb	O88561.
PRIDE	O88561.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	26568.
KEGG	mmu:26568.
Organism-specific databases
CTD	11000.
MGI	MGI:1347358. Slc27a3.
Phylogenomic databases
eggNOG	COG0318.
HOGENOM	HOG000044189.
HOVERGEN	HBG005642.
InParanoid	O88561.
KO	K08772.
OrthoDB	EOG4RXXZN.
Enzyme and pathway databases
BRENDA	6.2.1.3. 3474.
Gene expression databases
Genevestigator	O88561.
GermOnline	ENSMUSG00000027932. Mus musculus.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304647.
SOURCE	Search...

Entry information

Entry name

S27A3_MOUSE

Accession

Primary (citable) accession number: O88561
Secondary accession number(s): Q6PAU1, Q8BK70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program