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Protein

Claudin-2

Gene

Cldn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • structural molecule activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Protein family/group databases

TCDBi1.H.1.1.11. the claudin tight junction (claudin1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-2
Gene namesi
Name:Cldn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1276110. Cldn2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 11614CytoplasmicSequence analysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence analysisAdd
BLAST
Topological domaini138 – 16225ExtracellularSequence analysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Topological domaini184 – 23047CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Claudin-2PRO_0000144735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 64By similarity
Cross-linki218 – 218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei223 – 2231PhosphoserineCombined sources

Post-translational modificationi

The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88552.
PeptideAtlasiO88552.
PRIDEiO88552.

PTM databases

iPTMnetiO88552.
PhosphoSiteiO88552.

Expressioni

Tissue specificityi

Expressed in the kidney, liver and intestine, with higher levels in the ileum than in the jejunum. Low levels in the brain.2 Publications

Inductioni

By CDX1 and CDX2. Induction by CDX2, but not CDX1, is potentiated by TCF1.1 Publication

Gene expression databases

BgeeiENSMUSG00000047230.
CleanExiMM_CLDN2.
ExpressionAtlasiO88552. baseline and differential.
GenevisibleiO88552. MM.

Interactioni

Subunit structurei

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.2 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

MINTiMINT-113407.
STRINGi10090.ENSMUSP00000054219.

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi152 – 1543Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P5HX-ray3.381/2/3/4/P/Q/R/S/T/U/V/W/X/Y/Z141-160[»]
ProteinModelPortaliO88552.
SMRiO88552. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2302Interactions with TJP1, TJP2 and TJP3By similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG06. Eukaryota.
ENOG410YBJ7. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO88552.
KOiK06087.
OMAiRTSSYVG.
OrthoDBiEOG091G0MX2.
PhylomeDBiO88552.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR005411. Claudin2.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01589. CLAUDIN2.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLGVQLVG YILGLLGLLG TSIAMLLPNW RTSSYVGASI VTAVGFSKGL
60 70 80 90 100
WMECATHSTG ITQCDIYSTL LGLPADIQAA QAMMVTSSAM SSLACIISVV
110 120 130 140 150
GMRCTVFCQD SRAKDRVAVV GGVFFILGGI LGFIPVAWNL HGILRDFYSP
160 170 180 190 200
LVPDSMKFEI GEALYLGIIS ALFSLVAGVI LCFSCSPQGN RTNYYDGYQA
210 220 230
QPLATRSSPR SAQQPKAKSE FNSYSLTGYV
Length:230
Mass (Da):24,484
Last modified:November 1, 1998 - v1
Checksum:i38A7C074A1E0D5D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072128 mRNA. Translation: AAC27079.1.
AK004990 mRNA. Translation: BAB23725.1.
BC015252 mRNA. Translation: AAH15252.1.
BC085494 mRNA. Translation: AAH85494.1.
CCDSiCCDS30437.1.
RefSeqiNP_057884.1. NM_016675.4.
XP_006528553.1. XM_006528490.2.
UniGeneiMm.117068.

Genome annotation databases

EnsembliENSMUST00000054889; ENSMUSP00000054219; ENSMUSG00000047230.
GeneIDi12738.
KEGGimmu:12738.
UCSCiuc009ukl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072128 mRNA. Translation: AAC27079.1.
AK004990 mRNA. Translation: BAB23725.1.
BC015252 mRNA. Translation: AAH15252.1.
BC085494 mRNA. Translation: AAH85494.1.
CCDSiCCDS30437.1.
RefSeqiNP_057884.1. NM_016675.4.
XP_006528553.1. XM_006528490.2.
UniGeneiMm.117068.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P5HX-ray3.381/2/3/4/P/Q/R/S/T/U/V/W/X/Y/Z141-160[»]
ProteinModelPortaliO88552.
SMRiO88552. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-113407.
STRINGi10090.ENSMUSP00000054219.

Protein family/group databases

TCDBi1.H.1.1.11. the claudin tight junction (claudin1) family.

PTM databases

iPTMnetiO88552.
PhosphoSiteiO88552.

Proteomic databases

PaxDbiO88552.
PeptideAtlasiO88552.
PRIDEiO88552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054889; ENSMUSP00000054219; ENSMUSG00000047230.
GeneIDi12738.
KEGGimmu:12738.
UCSCiuc009ukl.2. mouse.

Organism-specific databases

CTDi9075.
MGIiMGI:1276110. Cldn2.

Phylogenomic databases

eggNOGiENOG410IG06. Eukaryota.
ENOG410YBJ7. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiO88552.
KOiK06087.
OMAiRTSSYVG.
OrthoDBiEOG091G0MX2.
PhylomeDBiO88552.
TreeFamiTF331936.

Miscellaneous databases

ChiTaRSiCldn2. mouse.
PROiO88552.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047230.
CleanExiMM_CLDN2.
ExpressionAtlasiO88552. baseline and differential.
GenevisibleiO88552. MM.

Family and domain databases

InterProiIPR006187. Claudin.
IPR005411. Claudin2.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01589. CLAUDIN2.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLD2_MOUSE
AccessioniPrimary (citable) accession number: O88552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.