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Protein

COP9 signalosome complex subunit 3

Gene

Cops3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Essential to maintain the survival of epiblast cells and thus the development of the postimplantation embryo.1 Publication

GO - Biological processi

  • cullin deneddylation Source: MGI
  • in utero embryonic development Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 3
Short name:
SGN3
Short name:
Signalosome subunit 3
Alternative name(s):
JAB1-containing signalosome subunit 3
Gene namesi
Name:Cops3
Synonyms:Csn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1349409. Cops3.

Subcellular locationi

GO - Cellular componenti

  • COP9 signalosome Source: MGI
  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Disruption phenotypei

Embryos arrest after 5.5 dpc and resorb by 8.5 dpc mainly due to increased cell death.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 423422COP9 signalosome complex subunit 3PRO_0000120979Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei407 – 4071PhosphoserineCombined sources
Modified residuei410 – 4101PhosphoserineCombined sources
Modified residuei423 – 4231PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88543.
PaxDbiO88543.
PRIDEiO88543.

PTM databases

iPTMnetiO88543.
PhosphoSiteiO88543.
SwissPalmiO88543.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiO88543.
CleanExiMM_CSN3.
ExpressionAtlasiO88543. baseline and differential.
GenevisibleiO88543. MM.

Interactioni

Subunit structurei

Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS4 and COPS8. Interacts with CK2 and PKD. Interacts with the translation initiation factor EIF3S6 and IKBKG (By similarity).By similarity

Protein-protein interaction databases

BioGridi205027. 5 interactions.
IntActiO88543. 4 interactions.
MINTiMINT-4092168.
STRINGi10090.ENSMUSP00000019517.

Structurei

3D structure databases

ProteinModelPortaliO88543.
SMRiO88543. Positions 3-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 362168PCIAdd
BLAST

Sequence similaritiesi

Belongs to the CSN3 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiKOG2582. Eukaryota.
ENOG410XRKY. LUCA.
HOGENOMiHOG000030451.
HOVERGENiHBG051135.
InParanoidiO88543.
KOiK12177.
OMAiSKHSETF.
OrthoDBiEOG7ZPNJV.
PhylomeDBiO88543.
TreeFamiTF101146.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASALEQFVN SVRQLSAQGQ MTQLCELINK SGELLAKNLS HLDTVLGALD
60 70 80 90 100
VQEHSLGVLA VLFVKFSMPS VPDFETLFSQ VQLFISTCNG EHIRYATDTF
110 120 130 140 150
AGLCHQLTNA LVERKQPLRG IGILKQAIDK MQMNTNQLTS VHADLCQLCL
160 170 180 190 200
LAKCFKPALP YLDVDMMDIC KENGAYDAKH FLCYYYYGGM IYTGLKNFER
210 220 230 240 250
ALYFYEQAIT TPAMAVSHIM LESYKKYILV SLILLGKVQQ LPKYTSQIVG
260 270 280 290 300
RFIKPLSNAY HELAQVYSTN NPSELRNLVS KHSETFTRDN NMGLVKQCLS
310 320 330 340 350
SLYKKNIQRL TKTFLTLSLQ DMASRVQLSG PQEAEKYVLH MIEDGEIFAS
360 370 380 390 400
INQKDGMVSF HDNPEKYNNP AMLHNIDQEM LKCIELDERL KAMDQEITVN
410 420
PQFVQKSMGS QEDDSGNKPS SYS
Length:423
Mass (Da):47,832
Last modified:January 23, 2007 - v3
Checksum:i2FDCCDB98A5168FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071313 mRNA. Translation: AAC33900.1.
BC068179 mRNA. Translation: AAH68179.1.
CCDSiCCDS24778.1.
RefSeqiNP_036121.1. NM_011991.1.
UniGeneiMm.40.

Genome annotation databases

EnsembliENSMUST00000019517; ENSMUSP00000019517; ENSMUSG00000019373.
GeneIDi26572.
KEGGimmu:26572.
UCSCiuc007jfa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071313 mRNA. Translation: AAC33900.1.
BC068179 mRNA. Translation: AAH68179.1.
CCDSiCCDS24778.1.
RefSeqiNP_036121.1. NM_011991.1.
UniGeneiMm.40.

3D structure databases

ProteinModelPortaliO88543.
SMRiO88543. Positions 3-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205027. 5 interactions.
IntActiO88543. 4 interactions.
MINTiMINT-4092168.
STRINGi10090.ENSMUSP00000019517.

PTM databases

iPTMnetiO88543.
PhosphoSiteiO88543.
SwissPalmiO88543.

Proteomic databases

EPDiO88543.
PaxDbiO88543.
PRIDEiO88543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019517; ENSMUSP00000019517; ENSMUSG00000019373.
GeneIDi26572.
KEGGimmu:26572.
UCSCiuc007jfa.1. mouse.

Organism-specific databases

CTDi8533.
MGIiMGI:1349409. Cops3.

Phylogenomic databases

eggNOGiKOG2582. Eukaryota.
ENOG410XRKY. LUCA.
HOGENOMiHOG000030451.
HOVERGENiHBG051135.
InParanoidiO88543.
KOiK12177.
OMAiSKHSETF.
OrthoDBiEOG7ZPNJV.
PhylomeDBiO88543.
TreeFamiTF101146.

Enzyme and pathway databases

ReactomeiR-MMU-5696394. DNA Damage Recognition in GG-NER.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.

Miscellaneous databases

NextBioi304659.
PROiO88543.
SOURCEiSearch...

Gene expression databases

BgeeiO88543.
CleanExiMM_CSN3.
ExpressionAtlasiO88543. baseline and differential.
GenevisibleiO88543. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complex."
    Wei N., Tsuge T., Serino G., Dohmae N., Takio K., Matsui M., Deng X.-W.
    Curr. Biol. 8:919-922(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE CSN COMPLEX.
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "COP9 signalosome subunit 3 is essential for maintenance of cell proliferation in the mouse embryonic epiblast."
    Yan J., Walz K., Nakamura H., Carattini-Rivera S., Zhao Q., Vogel H., Wei N., Justice M.J., Bradley A., Lupski J.R.
    Mol. Cell. Biol. 23:6798-6808(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407 AND SER-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCSN3_MOUSE
AccessioniPrimary (citable) accession number: O88543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.