Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger RNA-binding protein

Gene

Zfr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in postimplantation and gastrulation stages of development. Binds to DNA and RNA. Involved in the nucleocytoplasmic shuttling of STAU2 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger RNA-binding protein
Gene namesi
Name:Zfr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1341890. Zfr.

Subcellular locationi

  • Nucleus
  • Cytoplasm
  • Cytoplasmic granule By similarity
  • Chromosome By similarity

  • Note: Associated with chromosome foci in meiotic cells. Localizes in somatodendritic compartment of primary hippocampal neurons (By similarity). Colocalizes with STAU2 in several cytosolic RNA granules (By similarity). Associated with chromosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003127201 – 1074Zinc finger RNA-binding proteinAdd BLAST1074

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei509N6-acetyllysineCombined sources1
Modified residuei516N6-acetyllysineCombined sources1
Modified residuei1054PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88532.
MaxQBiO88532.
PaxDbiO88532.
PeptideAtlasiO88532.
PRIDEiO88532.

PTM databases

iPTMnetiO88532.
PhosphoSitePlusiO88532.

Expressioni

Tissue specificityi

Expressed in Sertoli cells, spermatocytes, primary and growing oocytes and granulosa cells (at protein level). Expressed in testis, ovary and brain.2 Publications

Developmental stagei

Expressed in embryos at 5.5, 6.5 and 7 dpc (at protein level). Expressed in the trophoectoderm cells and inner cell mass of blastocysts (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022201.
CleanExiMM_ZFR.
ExpressionAtlasiO88532. baseline and differential.
GenevisibleiO88532. MM.

Interactioni

Subunit structurei

Found in a cytoplasmic mRNP complex with STAU2. Does not interact with STAU1 (By similarity). Interacts with STAU2.By similarity1 Publication

Protein-protein interaction databases

BioGridi204689. 3 interactors.
IntActiO88532. 4 interactors.
MINTiMINT-1753461.
STRINGi10090.ENSMUSP00000118911.

Structurei

3D structure databases

ProteinModelPortaliO88532.
SMRiO88532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini703 – 1073DZFPROSITE-ProRule annotationAdd BLAST371

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 302Ala-richAdd BLAST267

Sequence similaritiesi

Contains 1 DZF domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3792. Eukaryota.
ENOG410XPBY. LUCA.
GeneTreeiENSGT00550000074528.
HOGENOMiHOG000232147.
HOVERGENiHBG108765.
InParanoidiO88532.
KOiK13203.
OMAiPQMSQRF.
OrthoDBiEOG091G022L.
PhylomeDBiO88532.
TreeFamiTF320194.

Family and domain databases

InterProiIPR006561. DZF_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF07528. DZF. 1 hit.
[Graphical view]
SMARTiSM00572. DZF. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 3 hits.
[Graphical view]
PROSITEiPS51703. DZF. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPICPVVSF TYVPSRLGED AKMATGNYFG FTHSGAAAAA AAAQYSQQPA
60 70 80 90 100
SGVAYSHPTT VASYTVHQAP VAAHTVTAAY APAAATVAVA RPAPVAVAAA
110 120 130 140 150
ATAAAYGGYP TAHTATDYGY TQRQQEAPPP PPPATTQNYQ DSYSYVRSTA
160 170 180 190 200
PAVAYDSKQY YQQPTATAAA VAAAAQPQPS VAETYYQTAP KAGYSQGATQ
210 220 230 240 250
YTQAQQARQV TAIKPATPSP ATTTFSIYPV SSTVQPVAAA ATVVPSYTQS
260 270 280 290 300
ATYSTTAVTY SGTSYSGYEA AVYSAASSYY QQQQQQQKQA AAAAAAAAAT
310 320 330 340 350
AAWTGTTFTK KTPFQNKQLK PKQPPKPPQI HYCDVCKISC AGPQTYKEHL
360 370 380 390 400
EGQKHKKKEA ALKASQNTSS SNNSTRGTQN QLRCELCDVS CTGADAYAAH
410 420 430 440 450
IRGAKHQKVV KLHTKLGKPI PSTEPNVVSQ ATSSTAASAS KPTASPSSIG
460 470 480 490 500
ASNCTLNTSS IATSSVKGLS TTGNSSLNST SNTKVSAIPT NMAAKKTSTP
510 520 530 540 550
KINFVGGNKL QSTGNKTEDL KGIDCVKNTP AASAVQIPEV KQDAGSEPVT
560 570 580 590 600
PASLAALQSD VQPVGHDYVE EVRNDEGKVI RFHCKLCECS FNDPNAKEMH
610 620 630 640 650
LKGRRHRLQY KKKVNPDLQV EVKPSIRARK IQEEKMRKQM QKEEYWRRRE
660 670 680 690 700
EEERWRMEIR RYEEDMYWRR MEEEQHHWDD RRRMPDGGYP HGPPGPLGLL
710 720 730 740 750
GVRPGMPPQP QGPAPLRRPD SSDDRYVMTK HATIYPTEEE LQAVQKIVSI
760 770 780 790 800
TERALKLVSD SLSEHEKSKN KEGDDKKEGG KDRALKGVLR VGVLAKGLLL
810 820 830 840 850
RGDRNVNLVL LCSEKPSKSL LSRIAENLPK QLAVISPEKY DIKCAVSEAA
860 870 880 890 900
IILNSCVEPK MQVTITLTSP IIREENMREG DVTSGMVKDP PDVLDRQKCL
910 920 930 940 950
DALAALRHAK WFQARANGLQ SCVIIIRILR DLCQRVPTWS DFPSWAMELL
960 970 980 990 1000
VEKAISSASS PQSPGDALRR VFECISSGII LKGSPGLLDP CEKDPFDTLA
1010 1020 1030 1040 1050
TMTDQQREDI TSSAQFALRL LAFRQIHKVL GMDPLPQMNQ RFNIHNNRKR
1060 1070
RRDSDGVDGF EAEGKKDKKD YDNF
Length:1,074
Mass (Da):116,859
Last modified:December 4, 2007 - v2
Checksum:iF0CE7B9F84A79A07
GO

Sequence cautioni

The sequence AAC25762 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH06962 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH38599 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH38599 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH58570 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH58570 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC28897 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti355H → K in AAH58570 (PubMed:15489334).Curated1
Sequence conflicti355H → K in AAH38599 (PubMed:15489334).Curated1
Sequence conflicti458T → S in BAC28897 (PubMed:16141072).Curated1
Sequence conflicti877M → I in BAB27548 (PubMed:16141072).Curated1
Sequence conflicti1016F → K in AAC25762 (PubMed:10072773).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071059 mRNA. Translation: AAC25762.1. Different initiation.
BC006962 mRNA. Translation: AAH06962.1. Different initiation.
BC038599 mRNA. Translation: AAH38599.1. Sequence problems.
BC058570 mRNA. Translation: AAH58570.1. Sequence problems.
AK011329 mRNA. Translation: BAB27548.1.
AK034963 mRNA. Translation: BAC28897.1. Different initiation.
AK158938 mRNA. Translation: BAE34733.1.
CCDSiCCDS37044.2.
PIRiT14343.
RefSeqiNP_035897.2. NM_011767.2.
UniGeneiMm.273496.

Genome annotation databases

EnsembliENSMUST00000122941; ENSMUSP00000118911; ENSMUSG00000022201.
GeneIDi22763.
KEGGimmu:22763.
UCSCiuc007vhk.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071059 mRNA. Translation: AAC25762.1. Different initiation.
BC006962 mRNA. Translation: AAH06962.1. Different initiation.
BC038599 mRNA. Translation: AAH38599.1. Sequence problems.
BC058570 mRNA. Translation: AAH58570.1. Sequence problems.
AK011329 mRNA. Translation: BAB27548.1.
AK034963 mRNA. Translation: BAC28897.1. Different initiation.
AK158938 mRNA. Translation: BAE34733.1.
CCDSiCCDS37044.2.
PIRiT14343.
RefSeqiNP_035897.2. NM_011767.2.
UniGeneiMm.273496.

3D structure databases

ProteinModelPortaliO88532.
SMRiO88532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204689. 3 interactors.
IntActiO88532. 4 interactors.
MINTiMINT-1753461.
STRINGi10090.ENSMUSP00000118911.

PTM databases

iPTMnetiO88532.
PhosphoSitePlusiO88532.

Proteomic databases

EPDiO88532.
MaxQBiO88532.
PaxDbiO88532.
PeptideAtlasiO88532.
PRIDEiO88532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000122941; ENSMUSP00000118911; ENSMUSG00000022201.
GeneIDi22763.
KEGGimmu:22763.
UCSCiuc007vhk.3. mouse.

Organism-specific databases

CTDi51663.
MGIiMGI:1341890. Zfr.

Phylogenomic databases

eggNOGiKOG3792. Eukaryota.
ENOG410XPBY. LUCA.
GeneTreeiENSGT00550000074528.
HOGENOMiHOG000232147.
HOVERGENiHBG108765.
InParanoidiO88532.
KOiK13203.
OMAiPQMSQRF.
OrthoDBiEOG091G022L.
PhylomeDBiO88532.
TreeFamiTF320194.

Miscellaneous databases

ChiTaRSiZfr. mouse.
PROiO88532.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022201.
CleanExiMM_ZFR.
ExpressionAtlasiO88532. baseline and differential.
GenevisibleiO88532. MM.

Family and domain databases

InterProiIPR006561. DZF_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF07528. DZF. 1 hit.
[Graphical view]
SMARTiSM00572. DZF. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
SM00451. ZnF_U1. 3 hits.
[Graphical view]
PROSITEiPS51703. DZF. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFR_MOUSE
AccessioniPrimary (citable) accession number: O88532
Secondary accession number(s): Q3TY30
, Q8BS85, Q8CGG5, Q91VZ0, Q9CT34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockout mice form mesoderm but are delayed in their development and fail to form normal anterior embryonic structures. Show both an increase in programmed cell death and a decrease in mitotic index, especially in the region of the distal tip of the embryonic ectoderm. Show also a reduction in apical vacuoles in the columnar visceral endoderm cells in the extraembryonic region. Knockout mice die by 8 to 9 days of gestation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.