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O88516

- DLL3_MOUSE

UniProt

O88516 - DLL3_MOUSE

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Protein

Delta-like protein 3

Gene

Dll3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

GO - Molecular functioni

  1. Notch binding Source: UniProtKB

GO - Biological processi

  1. cell fate determination Source: UniProtKB
  2. compartment pattern specification Source: MGI
  3. in utero embryonic development Source: UniProtKB
  4. negative regulation of neurogenesis Source: MGI
  5. nervous system development Source: UniProtKB
  6. Notch signaling pathway Source: UniProtKB
  7. paraxial mesoderm development Source: MGI
  8. skeletal system development Source: UniProtKB
  9. somitogenesis Source: MGI
  10. tissue development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Notch signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-like protein 3
Alternative name(s):
Drosophila Delta homolog 3
Short name:
Delta3
Short name:
M-Delta-3
Gene namesi
Name:Dll3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1096877. Dll3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 490458ExtracellularSequence AnalysisAdd
BLAST
Transmembranei491 – 51121HelicalSequence AnalysisAdd
BLAST
Topological domaini512 – 59281CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 592560Delta-like protein 3PRO_0000007510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi218 ↔ 229PROSITE-ProRule annotation
Disulfide bondi222 ↔ 235PROSITE-ProRule annotation
Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
Disulfide bondi276 ↔ 287PROSITE-ProRule annotation
Disulfide bondi281 ↔ 296PROSITE-ProRule annotation
Disulfide bondi298 ↔ 307PROSITE-ProRule annotation
Disulfide bondi314 ↔ 325PROSITE-ProRule annotation
Disulfide bondi319 ↔ 337PROSITE-ProRule annotation
Disulfide bondi339 ↔ 348PROSITE-ProRule annotation
Disulfide bondi355 ↔ 366PROSITE-ProRule annotation
Disulfide bondi360 ↔ 375PROSITE-ProRule annotation
Disulfide bondi377 ↔ 386PROSITE-ProRule annotation
Disulfide bondi393 ↔ 404PROSITE-ProRule annotation
Disulfide bondi398 ↔ 413PROSITE-ProRule annotation
Disulfide bondi415 ↔ 424PROSITE-ProRule annotation
Disulfide bondi431 ↔ 442PROSITE-ProRule annotation
Disulfide bondi436 ↔ 451PROSITE-ProRule annotation
Disulfide bondi453 ↔ 462PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.By similarity

Keywords - PTMi

Disulfide bond, Ubl conjugation

Proteomic databases

MaxQBiO88516.
PRIDEiO88516.

Expressioni

Tissue specificityi

Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis.

Gene expression databases

BgeeiO88516.
CleanExiMM_DLL3.
ExpressionAtlasiO88516. baseline and differential.
GenevestigatoriO88516.

Interactioni

Subunit structurei

Can bind and activate Notch-1 or another Notch receptor.Curated

Protein-protein interaction databases

BioGridi199233. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO88516.
SMRiO88516. Positions 25-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini174 – 21340DSLAdd
BLAST
Domaini214 – 24734EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 30837EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini310 – 34940EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini351 – 38737EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini389 – 42537EGF-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini427 – 46337EGF-like 6PROSITE-ProRule annotationAdd
BLAST

Domaini

The DSL domain is required for binding to the Notch receptor.

Sequence similaritiesi

Contains 1 DSL domain.Curated
Contains 6 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG244272.
HOGENOMiHOG000267024.
HOVERGENiHBG007139.
InParanoidiO88516.
KOiK06051.
OMAiDECRCLE.
OrthoDBiEOG7P8P7B.
PhylomeDBiO88516.
TreeFamiTF351835.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011651. Notch_ligand_N.
[Graphical view]
PfamiPF00008. EGF. 3 hits.
PF12661. hEGF. 2 hits.
PF07657. MNNL. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 6 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00022. EGF_1. 6 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: O88516-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSLQVSPLS QTLILAFLLP QALPAGVFEL QIHSFGPGPG LGTPRSPCNA
60 70 80 90 100
RGPCRLFFRV CLKPGVSQEA TESLCALGAA LSTSVPVYTE HPGESAAALP
110 120 130 140 150
LPDGLVRVPF RDAWPGTFSL VIETWREQLG EHAGGPAWNL LARVVGRRRL
160 170 180 190 200
AAGGPWARDV QRTGTWELHF SYRARCEPPA VGAACARLCR SRSAPSRCGP
210 220 230 240 250
GLRPCTPFPD ECEAPSVCRP GCSPEHGYCE EPDECRCLEG WTGPLCTVPV
260 270 280 290 300
STSSCLNSRV PGPASTGCLL PGPGPCDGNP CANGGSCSET SGSFECACPR
310 320 330 340 350
GFYGLRCEVS GVTCADGPCF NGGLCVGGED PDSAYVCHCP PGFQGSNCEK
360 370 380 390 400
RVDRCSLQPC QNGGLCLDLG HALRCRCRAG FAGPRCEHDL DDCAGRACAN
410 420 430 440 450
GGTCVEGGGS RRCSCALGFG GRDCRERADP CASRPCAHGG RCYAHFSGLV
460 470 480 490 500
CACAPGYMGV RCEFAVRPDG ADAVPAAPRG LRQADPQRFL LPPALGLLVA
510 520 530 540 550
AGLAGAALLV IHVRRRGPGQ DTGTRLLSGT REPSVHTLPD ALNNLRLQDG
560 570 580 590
AGDGPSSSAD WNHPEDGDSR SIYVIPAPSI YAREDWLIQV LF
Length:592
Mass (Da):62,069
Last modified:November 1, 1998 - v1
Checksum:i1A84F8022E7E7DCC
GO
Isoform 1 (identifier: O88516-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     585-592: DWLIQVLF → A

Show »
Length:585
Mass (Da):61,125
Checksum:iEB276F608998D4E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941E → K in BAA33716. 1 PublicationCurated
Sequence conflicti401 – 4011G → A in CAA72637. (PubMed:9272948)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei585 – 5928DWLIQVLF → A in isoform 1. 3 PublicationsVSP_001376

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068865 Genomic DNA. Translation: AAC40170.1.
AF068865 Genomic DNA. Translation: AAC40169.1.
Y11895 mRNA. Translation: CAA72637.1.
AB013440 mRNA. Translation: BAA33716.1.
BC052002 mRNA. Translation: AAH52002.1.
CCDSiCCDS39856.1. [O88516-2]
RefSeqiNP_031892.2. NM_007866.2. [O88516-2]
UniGeneiMm.12896.

Genome annotation databases

EnsembliENSMUST00000108315; ENSMUSP00000103951; ENSMUSG00000003436. [O88516-2]
GeneIDi13389.
KEGGimmu:13389.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068865 Genomic DNA. Translation: AAC40170.1 .
AF068865 Genomic DNA. Translation: AAC40169.1 .
Y11895 mRNA. Translation: CAA72637.1 .
AB013440 mRNA. Translation: BAA33716.1 .
BC052002 mRNA. Translation: AAH52002.1 .
CCDSi CCDS39856.1. [O88516-2 ]
RefSeqi NP_031892.2. NM_007866.2. [O88516-2 ]
UniGenei Mm.12896.

3D structure databases

ProteinModelPortali O88516.
SMRi O88516. Positions 25-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199233. 1 interaction.

Proteomic databases

MaxQBi O88516.
PRIDEi O88516.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000108315 ; ENSMUSP00000103951 ; ENSMUSG00000003436 . [O88516-2 ]
GeneIDi 13389.
KEGGi mmu:13389.

Organism-specific databases

CTDi 10683.
MGIi MGI:1096877. Dll3.

Phylogenomic databases

eggNOGi NOG244272.
HOGENOMi HOG000267024.
HOVERGENi HBG007139.
InParanoidi O88516.
KOi K06051.
OMAi DECRCLE.
OrthoDBi EOG7P8P7B.
PhylomeDBi O88516.
TreeFami TF351835.

Miscellaneous databases

NextBioi 283752.
PROi O88516.
SOURCEi Search...

Gene expression databases

Bgeei O88516.
CleanExi MM_DLL3.
ExpressionAtlasi O88516. baseline and differential.
Genevestigatori O88516.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011651. Notch_ligand_N.
[Graphical view ]
Pfami PF00008. EGF. 3 hits.
PF12661. hEGF. 2 hits.
PF07657. MNNL. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 6 hits.
[Graphical view ]
SUPFAMi SSF57184. SSF57184. 1 hit.
PROSITEi PS00022. EGF_1. 6 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Dll3: a novel divergent Delta gene which may complement the function of other Delta homologues during early pattern formation in the mouse embryo."
    Dunwoodie S.L., Henrique D.M.P., Harrison S.M., Beddington R.S.P.
    Development 124:3065-3076(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6 X DBA.
    Tissue: Embryo.
  2. "The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries."
    Kusumi K., Sun E.S., Kerrebrock A.W., Bronson R.T., Chi D.-C., Bulotsky M.S., Spencer J.B., Birren B.W., Frankel W.N., Lander E.S.
    Nat. Genet. 19:274-278(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: 129/SvJ.
  3. "Specific expression of a divergent type of Delta in a set of earliest generated neurons including the prospective subplate neurons."
    Nakayama K., Nakayama N., Tomooka Y., Hayashi Y., Takahashi M.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Neural tube.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiDLL3_MOUSE
AccessioniPrimary (citable) accession number: O88516
Secondary accession number(s): O35675
, Q80W06, Q9QWL9, Q9QWZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: November 26, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3