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O88516

- DLL3_MOUSE

UniProt

O88516 - DLL3_MOUSE

Protein

Delta-like protein 3

Gene

Dll3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

    GO - Molecular functioni

    1. Notch binding Source: UniProtKB
    2. protein binding Source: MGI

    GO - Biological processi

    1. cell fate determination Source: UniProtKB
    2. compartment pattern specification Source: MGI
    3. in utero embryonic development Source: UniProtKB
    4. negative regulation of neurogenesis Source: MGI
    5. nervous system development Source: UniProtKB
    6. Notch signaling pathway Source: UniProtKB
    7. paraxial mesoderm development Source: MGI
    8. skeletal system development Source: UniProtKB
    9. somitogenesis Source: MGI
    10. tissue development Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Notch signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-like protein 3
    Alternative name(s):
    Drosophila Delta homolog 3
    Short name:
    Delta3
    Short name:
    M-Delta-3
    Gene namesi
    Name:Dll3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1096877. Dll3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 592560Delta-like protein 3PRO_0000007510Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi218 ↔ 229PROSITE-ProRule annotation
    Disulfide bondi222 ↔ 235PROSITE-ProRule annotation
    Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
    Disulfide bondi276 ↔ 287PROSITE-ProRule annotation
    Disulfide bondi281 ↔ 296PROSITE-ProRule annotation
    Disulfide bondi298 ↔ 307PROSITE-ProRule annotation
    Disulfide bondi314 ↔ 325PROSITE-ProRule annotation
    Disulfide bondi319 ↔ 337PROSITE-ProRule annotation
    Disulfide bondi339 ↔ 348PROSITE-ProRule annotation
    Disulfide bondi355 ↔ 366PROSITE-ProRule annotation
    Disulfide bondi360 ↔ 375PROSITE-ProRule annotation
    Disulfide bondi377 ↔ 386PROSITE-ProRule annotation
    Disulfide bondi393 ↔ 404PROSITE-ProRule annotation
    Disulfide bondi398 ↔ 413PROSITE-ProRule annotation
    Disulfide bondi415 ↔ 424PROSITE-ProRule annotation
    Disulfide bondi431 ↔ 442PROSITE-ProRule annotation
    Disulfide bondi436 ↔ 451PROSITE-ProRule annotation
    Disulfide bondi453 ↔ 462PROSITE-ProRule annotation

    Post-translational modificationi

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.By similarity

    Keywords - PTMi

    Disulfide bond, Ubl conjugation

    Proteomic databases

    PRIDEiO88516.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis.

    Gene expression databases

    ArrayExpressiO88516.
    BgeeiO88516.
    CleanExiMM_DLL3.
    GenevestigatoriO88516.

    Interactioni

    Subunit structurei

    Can bind and activate Notch-1 or another Notch receptor.Curated

    Protein-protein interaction databases

    BioGridi199233. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO88516.
    SMRiO88516. Positions 25-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 490458ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini512 – 59281CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei491 – 51121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini174 – 21340DSLAdd
    BLAST
    Domaini214 – 24734EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini272 – 30837EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini310 – 34940EGF-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini351 – 38737EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 42537EGF-like 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini427 – 46337EGF-like 6PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The DSL domain is required for binding to the Notch receptor.

    Sequence similaritiesi

    Contains 1 DSL domain.Curated
    Contains 6 EGF-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG244272.
    HOGENOMiHOG000267024.
    HOVERGENiHBG007139.
    InParanoidiO88516.
    KOiK06051.
    OMAiDECRCLE.
    OrthoDBiEOG7P8P7B.
    PhylomeDBiO88516.
    TreeFamiTF351835.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR011651. Notch_ligand_N.
    [Graphical view]
    PfamiPF00008. EGF. 3 hits.
    PF12661. hEGF. 2 hits.
    PF07657. MNNL. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 6 hits.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    PROSITEiPS00022. EGF_1. 6 hits.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 6 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: O88516-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSLQVSPLS QTLILAFLLP QALPAGVFEL QIHSFGPGPG LGTPRSPCNA    50
    RGPCRLFFRV CLKPGVSQEA TESLCALGAA LSTSVPVYTE HPGESAAALP 100
    LPDGLVRVPF RDAWPGTFSL VIETWREQLG EHAGGPAWNL LARVVGRRRL 150
    AAGGPWARDV QRTGTWELHF SYRARCEPPA VGAACARLCR SRSAPSRCGP 200
    GLRPCTPFPD ECEAPSVCRP GCSPEHGYCE EPDECRCLEG WTGPLCTVPV 250
    STSSCLNSRV PGPASTGCLL PGPGPCDGNP CANGGSCSET SGSFECACPR 300
    GFYGLRCEVS GVTCADGPCF NGGLCVGGED PDSAYVCHCP PGFQGSNCEK 350
    RVDRCSLQPC QNGGLCLDLG HALRCRCRAG FAGPRCEHDL DDCAGRACAN 400
    GGTCVEGGGS RRCSCALGFG GRDCRERADP CASRPCAHGG RCYAHFSGLV 450
    CACAPGYMGV RCEFAVRPDG ADAVPAAPRG LRQADPQRFL LPPALGLLVA 500
    AGLAGAALLV IHVRRRGPGQ DTGTRLLSGT REPSVHTLPD ALNNLRLQDG 550
    AGDGPSSSAD WNHPEDGDSR SIYVIPAPSI YAREDWLIQV LF 592
    Length:592
    Mass (Da):62,069
    Last modified:November 1, 1998 - v1
    Checksum:i1A84F8022E7E7DCC
    GO
    Isoform 1 (identifier: O88516-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         585-592: DWLIQVLF → A

    Show »
    Length:585
    Mass (Da):61,125
    Checksum:iEB276F608998D4E9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941E → K in BAA33716. 1 PublicationCurated
    Sequence conflicti401 – 4011G → A in CAA72637. (PubMed:9272948)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei585 – 5928DWLIQVLF → A in isoform 1. 3 PublicationsVSP_001376

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF068865 Genomic DNA. Translation: AAC40170.1.
    AF068865 Genomic DNA. Translation: AAC40169.1.
    Y11895 mRNA. Translation: CAA72637.1.
    AB013440 mRNA. Translation: BAA33716.1.
    BC052002 mRNA. Translation: AAH52002.1.
    CCDSiCCDS39856.1. [O88516-2]
    RefSeqiNP_031892.2. NM_007866.2. [O88516-2]
    UniGeneiMm.12896.

    Genome annotation databases

    EnsembliENSMUST00000108315; ENSMUSP00000103951; ENSMUSG00000003436. [O88516-2]
    GeneIDi13389.
    KEGGimmu:13389.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF068865 Genomic DNA. Translation: AAC40170.1 .
    AF068865 Genomic DNA. Translation: AAC40169.1 .
    Y11895 mRNA. Translation: CAA72637.1 .
    AB013440 mRNA. Translation: BAA33716.1 .
    BC052002 mRNA. Translation: AAH52002.1 .
    CCDSi CCDS39856.1. [O88516-2 ]
    RefSeqi NP_031892.2. NM_007866.2. [O88516-2 ]
    UniGenei Mm.12896.

    3D structure databases

    ProteinModelPortali O88516.
    SMRi O88516. Positions 25-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199233. 1 interaction.

    Proteomic databases

    PRIDEi O88516.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108315 ; ENSMUSP00000103951 ; ENSMUSG00000003436 . [O88516-2 ]
    GeneIDi 13389.
    KEGGi mmu:13389.

    Organism-specific databases

    CTDi 10683.
    MGIi MGI:1096877. Dll3.

    Phylogenomic databases

    eggNOGi NOG244272.
    HOGENOMi HOG000267024.
    HOVERGENi HBG007139.
    InParanoidi O88516.
    KOi K06051.
    OMAi DECRCLE.
    OrthoDBi EOG7P8P7B.
    PhylomeDBi O88516.
    TreeFami TF351835.

    Miscellaneous databases

    NextBioi 283752.
    PROi O88516.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88516.
    Bgeei O88516.
    CleanExi MM_DLL3.
    Genevestigatori O88516.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR011651. Notch_ligand_N.
    [Graphical view ]
    Pfami PF00008. EGF. 3 hits.
    PF12661. hEGF. 2 hits.
    PF07657. MNNL. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 6 hits.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    PROSITEi PS00022. EGF_1. 6 hits.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse Dll3: a novel divergent Delta gene which may complement the function of other Delta homologues during early pattern formation in the mouse embryo."
      Dunwoodie S.L., Henrique D.M.P., Harrison S.M., Beddington R.S.P.
      Development 124:3065-3076(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6 X DBA.
      Tissue: Embryo.
    2. "The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries."
      Kusumi K., Sun E.S., Kerrebrock A.W., Bronson R.T., Chi D.-C., Bulotsky M.S., Spencer J.B., Birren B.W., Frankel W.N., Lander E.S.
      Nat. Genet. 19:274-278(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: 129/SvJ.
    3. "Specific expression of a divergent type of Delta in a set of earliest generated neurons including the prospective subplate neurons."
      Nakayama K., Nakayama N., Tomooka Y., Hayashi Y., Takahashi M.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Neural tube.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiDLL3_MOUSE
    AccessioniPrimary (citable) accession number: O88516
    Secondary accession number(s): O35675
    , Q80W06, Q9QWL9, Q9QWZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3