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O88516 (DLL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-like protein 3
Alternative name(s):
Drosophila Delta homolog 3
Short name=Delta3
Short name=M-Delta-3
Gene names
Name:Dll3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

Subunit structure

Can bind and activate Notch-1 or another Notch receptor Probable.

Subcellular location

Membrane; Single-pass type I membrane protein Probable.

Tissue specificity

Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis.

Domain

The DSL domain is required for binding to the Notch receptor.

Post-translational modification

Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation By similarity.

Involvement in disease

A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities.

Sequence similarities

Contains 1 DSL domain.

Contains 6 EGF-like domains.

Ontologies

Keywords
   Biological processDifferentiation
Notch signaling pathway
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainEGF-like domain
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Non-traceable author statement Ref.1. Source: UniProtKB

cell fate determination

Non-traceable author statement Ref.1. Source: UniProtKB

compartment pattern specification

Inferred from mutant phenotype PubMed 12001066. Source: MGI

in utero embryonic development

Non-traceable author statement Ref.1. Source: UniProtKB

negative regulation of neurogenesis

Inferred from direct assay Ref.1. Source: MGI

nervous system development

Non-traceable author statement Ref.1. Source: UniProtKB

paraxial mesoderm development

Inferred from mutant phenotype PubMed 15170697. Source: MGI

skeletal system development

Inferred from mutant phenotype PubMed 11923214. Source: UniProtKB

somitogenesis

Inferred from direct assay Ref.1. Source: MGI

tissue development

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionNotch binding

Non-traceable author statement Ref.1. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 16000382. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: O88516-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: O88516-2)

The sequence of this isoform differs from the canonical sequence as follows:
     585-592: DWLIQVLF → A

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 592560Delta-like protein 3
PRO_0000007510

Regions

Topological domain33 – 490458Extracellular Potential
Transmembrane491 – 51121Helical; Potential
Topological domain512 – 59281Cytoplasmic Potential
Domain174 – 21340DSL
Domain214 – 24734EGF-like 1
Domain272 – 30837EGF-like 2
Domain310 – 34940EGF-like 3
Domain351 – 38737EGF-like 4
Domain389 – 42537EGF-like 5
Domain427 – 46337EGF-like 6

Amino acid modifications

Disulfide bond218 ↔ 229 By similarity
Disulfide bond222 ↔ 235 By similarity
Disulfide bond237 ↔ 246 By similarity
Disulfide bond276 ↔ 287 By similarity
Disulfide bond281 ↔ 296 By similarity
Disulfide bond298 ↔ 307 By similarity
Disulfide bond314 ↔ 325 By similarity
Disulfide bond319 ↔ 337 By similarity
Disulfide bond339 ↔ 348 By similarity
Disulfide bond355 ↔ 366 By similarity
Disulfide bond360 ↔ 375 By similarity
Disulfide bond377 ↔ 386 By similarity
Disulfide bond393 ↔ 404 By similarity
Disulfide bond398 ↔ 413 By similarity
Disulfide bond415 ↔ 424 By similarity
Disulfide bond431 ↔ 442 By similarity
Disulfide bond436 ↔ 451 By similarity
Disulfide bond453 ↔ 462 By similarity

Natural variations

Alternative sequence585 – 5928DWLIQVLF → A in isoform 1.
VSP_001376

Experimental info

Sequence conflict941E → K in BAA33716. Ref.3
Sequence conflict4011G → A in CAA72637. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 1A84F8022E7E7DCC

FASTA59262,069
        10         20         30         40         50         60 
MVSLQVSPLS QTLILAFLLP QALPAGVFEL QIHSFGPGPG LGTPRSPCNA RGPCRLFFRV 

        70         80         90        100        110        120 
CLKPGVSQEA TESLCALGAA LSTSVPVYTE HPGESAAALP LPDGLVRVPF RDAWPGTFSL 

       130        140        150        160        170        180 
VIETWREQLG EHAGGPAWNL LARVVGRRRL AAGGPWARDV QRTGTWELHF SYRARCEPPA 

       190        200        210        220        230        240 
VGAACARLCR SRSAPSRCGP GLRPCTPFPD ECEAPSVCRP GCSPEHGYCE EPDECRCLEG 

       250        260        270        280        290        300 
WTGPLCTVPV STSSCLNSRV PGPASTGCLL PGPGPCDGNP CANGGSCSET SGSFECACPR 

       310        320        330        340        350        360 
GFYGLRCEVS GVTCADGPCF NGGLCVGGED PDSAYVCHCP PGFQGSNCEK RVDRCSLQPC 

       370        380        390        400        410        420 
QNGGLCLDLG HALRCRCRAG FAGPRCEHDL DDCAGRACAN GGTCVEGGGS RRCSCALGFG 

       430        440        450        460        470        480 
GRDCRERADP CASRPCAHGG RCYAHFSGLV CACAPGYMGV RCEFAVRPDG ADAVPAAPRG 

       490        500        510        520        530        540 
LRQADPQRFL LPPALGLLVA AGLAGAALLV IHVRRRGPGQ DTGTRLLSGT REPSVHTLPD 

       550        560        570        580        590 
ALNNLRLQDG AGDGPSSSAD WNHPEDGDSR SIYVIPAPSI YAREDWLIQV LF 

« Hide

Isoform 1 [UniParc].

Checksum: EB276F608998D4E9
Show »

FASTA58561,125

References

« Hide 'large scale' references
[1]"Mouse Dll3: a novel divergent Delta gene which may complement the function of other Delta homologues during early pattern formation in the mouse embryo."
Dunwoodie S.L., Henrique D.M.P., Harrison S.M., Beddington R.S.P.
Development 124:3065-3076(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6 X DBA.
Tissue: Embryo.
[2]"The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries."
Kusumi K., Sun E.S., Kerrebrock A.W., Bronson R.T., Chi D.-C., Bulotsky M.S., Spencer J.B., Birren B.W., Frankel W.N., Lander E.S.
Nat. Genet. 19:274-278(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: 129/SvJ.
[3]"Specific expression of a divergent type of Delta in a set of earliest generated neurons including the prospective subplate neurons."
Nakayama K., Nakayama N., Tomooka Y., Hayashi Y., Takahashi M.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Neural tube.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF068865 Genomic DNA. Translation: AAC40170.1.
AF068865 Genomic DNA. Translation: AAC40169.1.
Y11895 mRNA. Translation: CAA72637.1.
AB013440 mRNA. Translation: BAA33716.1.
BC052002 mRNA. Translation: AAH52002.1.
CCDSCCDS39856.1. [O88516-2]
RefSeqNP_031892.2. NM_007866.2. [O88516-2]
UniGeneMm.12896.

3D structure databases

ProteinModelPortalO88516.
SMRO88516. Positions 25-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199233. 1 interaction.

Proteomic databases

PRIDEO88516.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000108315; ENSMUSP00000103951; ENSMUSG00000003436. [O88516-2]
GeneID13389.
KEGGmmu:13389.

Organism-specific databases

CTD10683.
MGIMGI:1096877. Dll3.

Phylogenomic databases

eggNOGNOG244272.
HOGENOMHOG000267024.
HOVERGENHBG007139.
InParanoidO88516.
KOK06051.
OMADECRCLE.
OrthoDBEOG7P8P7B.
PhylomeDBO88516.
TreeFamTF351835.

Gene expression databases

ArrayExpressO88516.
BgeeO88516.
CleanExMM_DLL3.
GenevestigatorO88516.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR011651. Notch_ligand_N.
[Graphical view]
PfamPF00008. EGF. 3 hits.
PF12661. hEGF. 2 hits.
PF07657. MNNL. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 6 hits.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
PROSITEPS00022. EGF_1. 6 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio283752.
PROO88516.
SOURCESearch...

Entry information

Entry nameDLL3_MOUSE
AccessionPrimary (citable) accession number: O88516
Secondary accession number(s): O35675 expand/collapse secondary AC list , Q80W06, Q9QWL9, Q9QWZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot