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Protein

Delta-like protein 3

Gene

Dll3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • Notch binding Source: UniProtKB

GO - Biological processi

  • cell fate determination Source: UniProtKB
  • compartment pattern specification Source: MGI
  • in utero embryonic development Source: UniProtKB
  • negative regulation of neurogenesis Source: MGI
  • nervous system development Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB
  • paraxial mesoderm development Source: MGI
  • skeletal system development Source: UniProtKB
  • somitogenesis Source: MGI
  • tissue development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Notch signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-like protein 3
Alternative name(s):
Drosophila Delta homolog 3
Short name:
Delta3
Short name:
M-Delta-3
Gene namesi
Name:Dll3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1096877. Dll3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 490ExtracellularSequence analysisAdd BLAST458
Transmembranei491 – 511HelicalSequence analysisAdd BLAST21
Topological domaini512 – 592CytoplasmicSequence analysisAdd BLAST81

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000000751033 – 592Delta-like protein 3Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi218 ↔ 229PROSITE-ProRule annotation
Disulfide bondi222 ↔ 235PROSITE-ProRule annotation
Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
Disulfide bondi276 ↔ 287PROSITE-ProRule annotation
Disulfide bondi281 ↔ 296PROSITE-ProRule annotation
Disulfide bondi298 ↔ 307PROSITE-ProRule annotation
Disulfide bondi314 ↔ 325PROSITE-ProRule annotation
Disulfide bondi319 ↔ 337PROSITE-ProRule annotation
Disulfide bondi339 ↔ 348PROSITE-ProRule annotation
Disulfide bondi355 ↔ 366PROSITE-ProRule annotation
Disulfide bondi360 ↔ 375PROSITE-ProRule annotation
Disulfide bondi377 ↔ 386PROSITE-ProRule annotation
Disulfide bondi393 ↔ 404PROSITE-ProRule annotation
Disulfide bondi398 ↔ 413PROSITE-ProRule annotation
Disulfide bondi415 ↔ 424PROSITE-ProRule annotation
Disulfide bondi431 ↔ 442PROSITE-ProRule annotation
Disulfide bondi436 ↔ 451PROSITE-ProRule annotation
Disulfide bondi453 ↔ 462PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.By similarity

Keywords - PTMi

Disulfide bond, Ubl conjugation

Proteomic databases

MaxQBiO88516.
PaxDbiO88516.
PRIDEiO88516.

PTM databases

PhosphoSitePlusiO88516.

Expressioni

Tissue specificityi

Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis.

Gene expression databases

BgeeiENSMUSG00000003436.
CleanExiMM_DLL3.
ExpressionAtlasiO88516. baseline and differential.
GenevisibleiO88516. MM.

Interactioni

Subunit structurei

Can bind and activate Notch-1 or another Notch receptor.Curated

GO - Molecular functioni

  • Notch binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199233. 1 interactor.
STRINGi10090.ENSMUSP00000103951.

Structurei

3D structure databases

ProteinModelPortaliO88516.
SMRiO88516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 213DSLAdd BLAST40
Domaini214 – 247EGF-like 1PROSITE-ProRule annotationAdd BLAST34
Domaini272 – 308EGF-like 2PROSITE-ProRule annotationAdd BLAST37
Domaini310 – 349EGF-like 3PROSITE-ProRule annotationAdd BLAST40
Domaini351 – 387EGF-like 4PROSITE-ProRule annotationAdd BLAST37
Domaini389 – 425EGF-like 5PROSITE-ProRule annotationAdd BLAST37
Domaini427 – 463EGF-like 6PROSITE-ProRule annotationAdd BLAST37

Domaini

The DSL domain is required for binding to the Notch receptor.

Sequence similaritiesi

Contains 1 DSL domain.Curated
Contains 6 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
ENOG410XP6K. LUCA.
GeneTreeiENSGT00840000129708.
HOGENOMiHOG000267024.
HOVERGENiHBG007139.
InParanoidiO88516.
KOiK06051.
OMAiRGPVYTE.
PhylomeDBiO88516.
TreeFamiTF351835.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00008. EGF. 3 hits.
PF12661. hEGF. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 6 hits.
SM00179. EGF_CA. 5 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00022. EGF_1. 6 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O88516-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSLQVSPLS QTLILAFLLP QALPAGVFEL QIHSFGPGPG LGTPRSPCNA
60 70 80 90 100
RGPCRLFFRV CLKPGVSQEA TESLCALGAA LSTSVPVYTE HPGESAAALP
110 120 130 140 150
LPDGLVRVPF RDAWPGTFSL VIETWREQLG EHAGGPAWNL LARVVGRRRL
160 170 180 190 200
AAGGPWARDV QRTGTWELHF SYRARCEPPA VGAACARLCR SRSAPSRCGP
210 220 230 240 250
GLRPCTPFPD ECEAPSVCRP GCSPEHGYCE EPDECRCLEG WTGPLCTVPV
260 270 280 290 300
STSSCLNSRV PGPASTGCLL PGPGPCDGNP CANGGSCSET SGSFECACPR
310 320 330 340 350
GFYGLRCEVS GVTCADGPCF NGGLCVGGED PDSAYVCHCP PGFQGSNCEK
360 370 380 390 400
RVDRCSLQPC QNGGLCLDLG HALRCRCRAG FAGPRCEHDL DDCAGRACAN
410 420 430 440 450
GGTCVEGGGS RRCSCALGFG GRDCRERADP CASRPCAHGG RCYAHFSGLV
460 470 480 490 500
CACAPGYMGV RCEFAVRPDG ADAVPAAPRG LRQADPQRFL LPPALGLLVA
510 520 530 540 550
AGLAGAALLV IHVRRRGPGQ DTGTRLLSGT REPSVHTLPD ALNNLRLQDG
560 570 580 590
AGDGPSSSAD WNHPEDGDSR SIYVIPAPSI YAREDWLIQV LF
Length:592
Mass (Da):62,069
Last modified:November 1, 1998 - v1
Checksum:i1A84F8022E7E7DCC
GO
Isoform 1 (identifier: O88516-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     585-592: DWLIQVLF → A

Show »
Length:585
Mass (Da):61,125
Checksum:iEB276F608998D4E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94E → K in BAA33716 (Ref. 3) Curated1
Sequence conflicti401G → A in CAA72637 (PubMed:9272948).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001376585 – 592DWLIQVLF → A in isoform 1. 3 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068865 Genomic DNA. Translation: AAC40170.1.
AF068865 Genomic DNA. Translation: AAC40169.1.
Y11895 mRNA. Translation: CAA72637.1.
AB013440 mRNA. Translation: BAA33716.1.
BC052002 mRNA. Translation: AAH52002.1.
CCDSiCCDS39856.1. [O88516-2]
RefSeqiNP_031892.2. NM_007866.2. [O88516-2]
UniGeneiMm.12896.

Genome annotation databases

EnsembliENSMUST00000108315; ENSMUSP00000103951; ENSMUSG00000003436. [O88516-2]
GeneIDi13389.
KEGGimmu:13389.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068865 Genomic DNA. Translation: AAC40170.1.
AF068865 Genomic DNA. Translation: AAC40169.1.
Y11895 mRNA. Translation: CAA72637.1.
AB013440 mRNA. Translation: BAA33716.1.
BC052002 mRNA. Translation: AAH52002.1.
CCDSiCCDS39856.1. [O88516-2]
RefSeqiNP_031892.2. NM_007866.2. [O88516-2]
UniGeneiMm.12896.

3D structure databases

ProteinModelPortaliO88516.
SMRiO88516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199233. 1 interactor.
STRINGi10090.ENSMUSP00000103951.

PTM databases

PhosphoSitePlusiO88516.

Proteomic databases

MaxQBiO88516.
PaxDbiO88516.
PRIDEiO88516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108315; ENSMUSP00000103951; ENSMUSG00000003436. [O88516-2]
GeneIDi13389.
KEGGimmu:13389.

Organism-specific databases

CTDi10683.
MGIiMGI:1096877. Dll3.

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
ENOG410XP6K. LUCA.
GeneTreeiENSGT00840000129708.
HOGENOMiHOG000267024.
HOVERGENiHBG007139.
InParanoidiO88516.
KOiK06051.
OMAiRGPVYTE.
PhylomeDBiO88516.
TreeFamiTF351835.

Miscellaneous databases

PROiO88516.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003436.
CleanExiMM_DLL3.
ExpressionAtlasiO88516. baseline and differential.
GenevisibleiO88516. MM.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00008. EGF. 3 hits.
PF12661. hEGF. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 6 hits.
SM00179. EGF_CA. 5 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00022. EGF_1. 6 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLL3_MOUSE
AccessioniPrimary (citable) accession number: O88516
Secondary accession number(s): O35675
, Q80W06, Q9QWL9, Q9QWZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.