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O88513 (GEMI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Geminin
Gene names
Name:Gmnn
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Ref.1 Ref.3

Subunit structure

Homotetramer By similarity. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization By similarity. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Ref.3

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Mainly cytoplasmic but can be relocalized to the nucleus By similarity.

Developmental stage

Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Ref.1

Sequence similarities

Belongs to the geminin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 206206Geminin
PRO_0000148730

Regions

Region79 – 15880Necessary and sufficient for interaction with IDAS By similarity
Coiled coil91 – 14151 By similarity

Amino acid modifications

Modified residue271N6-acetyllysine By similarity
Modified residue631Phosphoserine By similarity
Modified residue641Phosphoserine By similarity

Secondary structure

1 ....... 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O88513 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: B87153A5D5BCF5CF

FASTA20623,300
        10         20         30         40         50         60 
MNLSMKQKQE GAQENVKNSP VPRRTLKMIQ PSADGSLVGR ENELPKGLFK RKLWDDQLAS 

        70         80         90        100        110        120 
QTSSCGPEAN ENKDVGDLTQ EAFDLISKEN PSSQYWKEVA EQRRKALYEA LKENEKLHKE 

       130        140        150        160        170        180 
IEQKDSEIAR LRKENKDLAE VAEHVQYMAE VIERLSNEPL DNFESPDSQE FDSEEEAVEY 

       190        200 
SELEDSGAGT CAEETVSSST DARPCT

« Hide

References

« Hide 'large scale' references
[1]"Geminin, an inhibitor of DNA replication, is degraded during mitosis."
McGarry T.J., Kirschner M.W.
Cell 93:1043-1053(1998) [PubMed: 9635433] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity."
Yanagi K., Mizuno T., You Z., Hanaoka F.
J. Biol. Chem. 277:40871-40880(2002) [PubMed: 12192004] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDT1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF068780 mRNA. Translation: AAC24228.1.
BC020061 mRNA. Translation: AAH20061.1.
IPIIPI00131716.
RefSeqNP_065592.1. NM_020567.2.
UniGeneMm.12239.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZXXX-ray2.80A/B/D/E79-157[»]
ProteinModelPortalO88513.
SMRO88513. Positions 79-156.
ModBaseSearch...

Protein-protein interaction databases

IntActO88513. 9 interactions.
MINTMINT-1172279.
STRINGO88513.

PTM databases

PhosphoSiteO88513.

Proteomic databases

PRIDEO88513.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006898; ENSMUSP00000006898; ENSMUSG00000006715.
ENSMUST00000110382; ENSMUSP00000106011; ENSMUSG00000006715.
GeneID57441.
KEGGmmu:57441.

Organism-specific databases

CTD51053.
MGIMGI:1927344. Gmnn.

Phylogenomic databases

eggNOGroNOG09336.
HOGENOMHBG714865.
HOVERGENHBG002965.
InParanoidO88513.
OMAPEHSENK.
OrthoDBEOG4JDH85.
PhylomeDBO88513.

Gene expression databases

ArrayExpressO88513.
BgeeO88513.
CleanExMM_GMNN.
GenevestigatorO88513.
GermOnlineENSMUSG00000006715. Mus musculus.

Family and domain databases

InterProIPR022786. Geminin.
[Graphical view]
KOK10749.
PANTHERPTHR13372. Geminin. 1 hit.
PfamPF07412. Geminin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313837.
SOURCESearch...

Entry information

Entry nameGEMI_MOUSE
AccessionPrimary (citable) accession number: O88513
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1998
Last modified: November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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