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O88513 (GEMI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Geminin
Gene names
Name:Gmnn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Ref.1 Ref.3

Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control By similarity. Ref.1 Ref.3

Subunit structure

Homotetramer. nteracts (via coiled-coil domain) with IDAS (via coiled-coil domain); this targets GMNN to the nucleus. The heterodimer formed by GMNN and MCIDAS has much lower affinity for CDT1 than the GMNN homodimer. Interacts with a subset of Hox proteins, affinity increasing from anterior to posterior types, the strongest interaction being with HOXB1, HOXC9 and HOXD10. Interacts with LRWD1 from G1/S to mitosis By similarity. Interacts with CDT1; this inhibits binding of the MCM complex to origins of replication. The complex with CDT1 exists in two forms, a "permissive" heterotrimer and an "inhibitory" heterohexamer. Ref.3 Ref.4

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Mainly cytoplasmic but can be relocalized to the nucleus By similarity.

Developmental stage

Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Ref.1

Post-translational modification

Phosphorylated during mitosis By similarity. Phosphorylation at Ser-181 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity By similarity.

Sequence similarities

Belongs to the geminin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 206206Geminin
PRO_0000148730

Regions

Region79 – 15880Necessary and sufficient for interaction with IDAS and CDT1 By similarity
Region167 – 18721Homeodomain binding By similarity
Coiled coil91 – 14151 Ref.4

Amino acid modifications

Modified residue271N6-acetyllysine By similarity
Modified residue631Phosphoserine By similarity
Modified residue641Phosphoserine By similarity
Modified residue1811Phosphoserine; by CK2 By similarity

Secondary structure

..... 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O88513 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: B87153A5D5BCF5CF

FASTA20623,300
        10         20         30         40         50         60 
MNLSMKQKQE GAQENVKNSP VPRRTLKMIQ PSADGSLVGR ENELPKGLFK RKLWDDQLAS 

        70         80         90        100        110        120 
QTSSCGPEAN ENKDVGDLTQ EAFDLISKEN PSSQYWKEVA EQRRKALYEA LKENEKLHKE 

       130        140        150        160        170        180 
IEQKDSEIAR LRKENKDLAE VAEHVQYMAE VIERLSNEPL DNFESPDSQE FDSEEEAVEY 

       190        200 
SELEDSGAGT CAEETVSSST DARPCT 

« Hide

References

« Hide 'large scale' references
[1]"Geminin, an inhibitor of DNA replication, is degraded during mitosis."
McGarry T.J., Kirschner M.W.
Cell 93:1043-1053(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity."
Yanagi K., Mizuno T., You Z., Hanaoka F.
J. Biol. Chem. 277:40871-40880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDT1.
[4]"Structural basis for inhibition of the replication licensing factor Cdt1 by geminin."
Lee C., Hong B., Choi J.M., Kim Y., Watanabe S., Ishimi Y., Enomoto T., Tada S., Kim Y., Cho Y.
Nature 430:913-917(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 79-157 IN COMPLEX WITH CDT1, DOMAIN COILED COIL, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF068780 mRNA. Translation: AAC24228.1.
BC020061 mRNA. Translation: AAH20061.1.
CCDSCCDS26378.1.
RefSeqNP_065592.1. NM_020567.2.
XP_006516785.1. XM_006516722.1.
XP_006516786.1. XM_006516723.1.
UniGeneMm.12239.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZXXX-ray2.80A/B/D/E79-157[»]
ProteinModelPortalO88513.
SMRO88513. Positions 79-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208298. 14 interactions.
DIPDIP-32562N.
IntActO88513. 9 interactions.
MINTMINT-1172279.
STRING10090.ENSMUSP00000006898.

PTM databases

PhosphoSiteO88513.

Proteomic databases

PaxDbO88513.
PRIDEO88513.

Protocols and materials databases

DNASU57441.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006898; ENSMUSP00000006898; ENSMUSG00000006715.
ENSMUST00000110382; ENSMUSP00000106011; ENSMUSG00000006715.
GeneID57441.
KEGGmmu:57441.
UCSCuc007pwg.1. mouse.

Organism-specific databases

CTD51053.
MGIMGI:1927344. Gmnn.

Phylogenomic databases

eggNOGNOG39688.
HOGENOMHOG000112711.
HOVERGENHBG002965.
InParanoidO88513.
KOK10749.
OMAVPEHSEN.
OrthoDBEOG7RFTJX.
PhylomeDBO88513.
TreeFamTF101171.

Gene expression databases

ArrayExpressO88513.
BgeeO88513.
CleanExMM_GMNN.
GenevestigatorO88513.

Family and domain databases

InterProIPR022786. Geminin_fam.
[Graphical view]
PfamPF07412. Geminin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO88513.
NextBio313837.
PROO88513.
SOURCESearch...

Entry information

Entry nameGEMI_MOUSE
AccessionPrimary (citable) accession number: O88513
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot