Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

STE20/SPS1-related proline-alanine-rich protein kinase

Gene

Stk39

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a mediator of stress-activated signals. Mediates the inhibiton of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATPPROSITE-ProRule annotation
Active sitei201 – 2011Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 869ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase binding Source: RGD
  • protein serine/threonine kinase activity Source: RGD
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20/SPS1-related proline-alanine-rich protein kinase (EC:2.7.11.1)
Short name:
Ste-20-related kinase
Alternative name(s):
Pancreatic serine/threonine-protein kinase
Short name:
PS/TK
Short name:
PSTK1
Serine/threonine-protein kinase 39
Gene namesi
Name:Stk39
Synonyms:Pask, Spak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621643. Stk39.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • basolateral plasma membrane Source: Ensembl
  • cytoplasm Source: RGD
  • cytoskeleton Source: RGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553STE20/SPS1-related proline-alanine-rich protein kinasePRO_0000086724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181Phosphoserine; by PKC/PRKCQBy similarity
Modified residuei358 – 3581N6-acetyllysineBy similarity
Modified residuei363 – 3631PhosphothreonineCombined sources
Modified residuei379 – 3791PhosphoserineBy similarity
Modified residuei380 – 3801PhosphoserineBy similarity
Modified residuei394 – 3941PhosphoserineCombined sources
Modified residuei402 – 4021PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-318 by PRKCQ.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO88506.
PRIDEiO88506.

PTM databases

iPTMnetiO88506.
PhosphoSiteiO88506.

Expressioni

Tissue specificityi

Highly expressed in testis followed by pancreas, kidney, heart and brain. Not expressed in skeletal muscle, liver, lung and spleen.

Developmental stagei

Expressed in the early gut and pancreatic epithelium, at E15 day localized to cells that will eventually become exocrine. Expressed in choroid plexus, developing myocardium, pancreatic epithelium and dorsal root ganglia.

Gene expression databases

ExpressionAtlasiO88506. baseline and differential.
GenevisibleiO88506. RN.

Interactioni

Subunit structurei

The phosphorylated form forms a complex with WNK2.By similarity

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034162.

Structurei

3D structure databases

ProteinModelPortaliO88506.
SMRiO88506. Positions 62-353, 456-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 346275Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi369 – 3757Nuclear localization signalSequence analysis
Motifi396 – 4005Caspase cleavage related site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 6249Ala/Pro-richAdd
BLAST
Compositional biasi22 – 309Poly-Ala

Domaini

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiO88506.
KOiK08835.
OMAiPPEKQPW.
OrthoDBiEOG715Q3W.
PhylomeDBiO88506.
TreeFamiTF105339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPSGSPVH VQLPQQAAPV TAAAAAPAAA TSAPAPAPAP AAPAAPAPAP
60 70 80 90 100
AAAPAPAPAA QAVGWPICRD AYELQEVIGS GATAVVQAAL CKPRQERVAI
110 120 130 140 150
KRINLEKCQT SMDELLKEIQ AMSQCSHPNV VTYYTSFVVK DELWLVMKLL
160 170 180 190 200
SGGSMLDIIK YIVNRGEHKN GVLEEAIIAT ILKEVLEGLD YLHRNGQIHR
210 220 230 240 250
DLKAGNILLG EDGSVQIADF GVSAFLATGG DVTRNKVRKT FVGTPCWMAP
260 270 280 290 300
EVMEQVRGYD FKADMWSFGI TAIELATGAA PYHKYPPMKV LMLTLQNDPP
310 320 330 340 350
TLETGVEDKE MMKKYGKSFR KLLSLCLQKD PSKRPTAAEL LKCKFFQKAK
360 370 380 390 400
NREYLIEKLL TRTPDIAQRA KKVRRVPGSS GHLHKTEDGD WEWSDDEMDE
410 420 430 440 450
KSEEGKAAAS QEKSRRVKEE NPEISVNAGG IPEQIQSLSV HDSQGQPNAN
460 470 480 490 500
EDYREGPCAV NLVLRLRNSR KELNDIRFEF TPGRDTADGV SQELFSAGLV
510 520 530 540 550
DGHDVVIVAA NLQKIVDDPK ALKTLTFKLA SGCDGAEIPD EVKLIGFAQL

SVS
Length:553
Mass (Da):60,051
Last modified:December 1, 2000 - v2
Checksum:i8BC1458AEA6F90C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111V → I in AAC23501 (PubMed:10990492).Curated
Sequence conflicti253 – 2531M → I in AAC23501 (PubMed:10990492).Curated
Sequence conflicti403 – 4031E → Q in AAC23501 (PubMed:10990492).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88190 mRNA. Translation: BAA26000.1.
AF068261 mRNA. Translation: AAC23501.1.
AF099990 mRNA. Translation: AAC72239.1.
RefSeqiNP_062235.1. NM_019362.1.
UniGeneiRn.34414.

Genome annotation databases

EnsembliENSRNOT00000037133; ENSRNOP00000034162; ENSRNOG00000024808.
GeneIDi54348.
KEGGirno:54348.
UCSCiRGD:621643. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88190 mRNA. Translation: BAA26000.1.
AF068261 mRNA. Translation: AAC23501.1.
AF099990 mRNA. Translation: AAC72239.1.
RefSeqiNP_062235.1. NM_019362.1.
UniGeneiRn.34414.

3D structure databases

ProteinModelPortaliO88506.
SMRiO88506. Positions 62-353, 456-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034162.

PTM databases

iPTMnetiO88506.
PhosphoSiteiO88506.

Proteomic databases

PaxDbiO88506.
PRIDEiO88506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000037133; ENSRNOP00000034162; ENSRNOG00000024808.
GeneIDi54348.
KEGGirno:54348.
UCSCiRGD:621643. rat.

Organism-specific databases

CTDi27347.
RGDi621643. Stk39.

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiO88506.
KOiK08835.
OMAiPPEKQPW.
OrthoDBiEOG715Q3W.
PhylomeDBiO88506.
TreeFamiTF105339.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Miscellaneous databases

PROiO88506.

Gene expression databases

ExpressionAtlasiO88506. baseline and differential.
GenevisibleiO88506. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel Ste20-related protein kinase enriched in neurons and transporting epithelia."
    Ushiro H., Tsutsumi T., Suzuki K., Kayahara T., Nakano K.
    Arch. Biochem. Biophys. 355:233-240(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Isolation and expression of PASK, a serine/threonine kinase, during rat embryonic development, with special emphasis on the pancreas."
    Miao N., Fung B., Sanchez R., Lydon J., Barker D., Pang K.
    J. Histochem. Cytochem. 48:1391-1400(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Pancreas.
  3. "SPAK, a STE20/SPS1-related kinase that activates the p38 pathway."
    Johnston A.M., Naselli G., Gonez L.J., Martin R.M., Harrison L.C., de Aizpurua H.J.
    Oncogene 19:4290-4297(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Insulinoma.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-363 AND SER-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTK39_RAT
AccessioniPrimary (citable) accession number: O88506
Secondary accession number(s): O70541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.