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Protein

STE20/SPS1-related proline-alanine-rich protein kinase

Gene

Stk39

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a mediator of stress-activated signals. Mediates the inhibiton of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATPPROSITE-ProRule annotation1
Active sitei201Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 86ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: ParkinsonsUK-UCL
  • protein kinase activity Source: RGD
  • protein kinase binding Source: RGD
  • protein serine/threonine kinase activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20/SPS1-related proline-alanine-rich protein kinase (EC:2.7.11.1)
Short name:
Ste-20-related kinase
Alternative name(s):
Pancreatic serine/threonine-protein kinase
Short name:
PS/TK
Short name:
PSTK1
Serine/threonine-protein kinase 39
Gene namesi
Name:Stk39
Synonyms:Pask, Spak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621643. Stk39.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867241 – 553STE20/SPS1-related proline-alanine-rich protein kinaseAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei318Phosphoserine; by PKC/PRKCQBy similarity1
Modified residuei358N6-acetyllysineBy similarity1
Modified residuei363PhosphothreonineCombined sources1
Modified residuei379PhosphoserineBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei394PhosphoserineCombined sources1
Modified residuei402PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-318 by PRKCQ.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO88506.
PRIDEiO88506.

PTM databases

iPTMnetiO88506.
PhosphoSitePlusiO88506.

Expressioni

Tissue specificityi

Highly expressed in testis followed by pancreas, kidney, heart and brain. Not expressed in skeletal muscle, liver, lung and spleen.

Developmental stagei

Expressed in the early gut and pancreatic epithelium, at E15 day localized to cells that will eventually become exocrine. Expressed in choroid plexus, developing myocardium, pancreatic epithelium and dorsal root ganglia.

Gene expression databases

BgeeiENSRNOG00000024808.
ExpressionAtlasiO88506. baseline and differential.
GenevisibleiO88506. RN.

Interactioni

Subunit structurei

The phosphorylated form forms a complex with WNK2.By similarity

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

ELMiO88506.
STRINGi10116.ENSRNOP00000034162.

Structurei

3D structure databases

ProteinModelPortaliO88506.
SMRiO88506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 346Protein kinasePROSITE-ProRule annotationAdd BLAST275

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi369 – 375Nuclear localization signalSequence analysis7
Motifi396 – 400Caspase cleavage related site5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 62Ala/Pro-richAdd BLAST49
Compositional biasi22 – 30Poly-Ala9

Domaini

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00900000140899.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiO88506.
KOiK08835.
OMAiLSVHDSQ.
OrthoDBiEOG091G0AKO.
PhylomeDBiO88506.
TreeFamiTF105339.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
PfamiView protein in Pfam
PF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

O88506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPSGSPVH VQLPQQAAPV TAAAAAPAAA TSAPAPAPAP AAPAAPAPAP
60 70 80 90 100
AAAPAPAPAA QAVGWPICRD AYELQEVIGS GATAVVQAAL CKPRQERVAI
110 120 130 140 150
KRINLEKCQT SMDELLKEIQ AMSQCSHPNV VTYYTSFVVK DELWLVMKLL
160 170 180 190 200
SGGSMLDIIK YIVNRGEHKN GVLEEAIIAT ILKEVLEGLD YLHRNGQIHR
210 220 230 240 250
DLKAGNILLG EDGSVQIADF GVSAFLATGG DVTRNKVRKT FVGTPCWMAP
260 270 280 290 300
EVMEQVRGYD FKADMWSFGI TAIELATGAA PYHKYPPMKV LMLTLQNDPP
310 320 330 340 350
TLETGVEDKE MMKKYGKSFR KLLSLCLQKD PSKRPTAAEL LKCKFFQKAK
360 370 380 390 400
NREYLIEKLL TRTPDIAQRA KKVRRVPGSS GHLHKTEDGD WEWSDDEMDE
410 420 430 440 450
KSEEGKAAAS QEKSRRVKEE NPEISVNAGG IPEQIQSLSV HDSQGQPNAN
460 470 480 490 500
EDYREGPCAV NLVLRLRNSR KELNDIRFEF TPGRDTADGV SQELFSAGLV
510 520 530 540 550
DGHDVVIVAA NLQKIVDDPK ALKTLTFKLA SGCDGAEIPD EVKLIGFAQL

SVS
Length:553
Mass (Da):60,051
Last modified:December 1, 2000 - v2
Checksum:i8BC1458AEA6F90C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11V → I in AAC23501 (PubMed:10990492).Curated1
Sequence conflicti253M → I in AAC23501 (PubMed:10990492).Curated1
Sequence conflicti403E → Q in AAC23501 (PubMed:10990492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88190 mRNA. Translation: BAA26000.1.
AF068261 mRNA. Translation: AAC23501.1.
AF099990 mRNA. Translation: AAC72239.1.
RefSeqiNP_062235.1. NM_019362.1.
UniGeneiRn.34414.

Genome annotation databases

EnsembliENSRNOT00000037133; ENSRNOP00000034162; ENSRNOG00000024808.
GeneIDi54348.
KEGGirno:54348.
UCSCiRGD:621643. rat.

Similar proteinsi

Entry informationi

Entry nameiSTK39_RAT
AccessioniPrimary (citable) accession number: O88506
Secondary accession number(s): O70541
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 22, 2017
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families