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O88502

- PDE8A_MOUSE

UniProt

O88502 - PDE8A_MOUSE

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Protein

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Gene

Pde8a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Enzyme regulationi

Inhibited by dipyridimole. Insensitive to selective PDE inhibitor rolipram and to the non-selective inhibitor, IBMX.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei551 – 5511Proton donorBy similarity
Metal bindingi555 – 5551Divalent metal cation 1By similarity
Metal bindingi591 – 5911Divalent metal cation 1By similarity
Metal bindingi592 – 5921Divalent metal cation 1By similarity
Metal bindingi592 – 5921Divalent metal cation 2By similarity
Metal bindingi720 – 7201Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
  2. phosphorelay signal transduction system Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (EC:3.1.4.53)
Short name:
MmPDE8
Gene namesi
Name:Pde8a
Synonyms:Pde8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1277116. Pde8a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8APRO_0000198839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei355 – 3551Phosphoserine; by PKABy similarity
Modified residuei452 – 4521PhosphoserineBy similarity
Modified residuei456 – 4561Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated at Ser-355 by PKA under elevated cAMP conditions, this enhances catalytic activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88502.
PRIDEiO88502.

PTM databases

PhosphoSiteiO88502.

Expressioni

Tissue specificityi

Highest levels in testis > eye > liver > skeletal muscle > heart > 7-day embryo > kidney > ovary > brain. In the testis, expressed specifically in the seminiferous epithelium in a spatial and temporal manner.

Developmental stagei

Levels of expression decrease sometime between embryo day 7 and day 11. In the testis, expression restricted to middle and late pachytene spermatocytes.

Gene expression databases

BgeeiO88502.
CleanExiMM_PDE8A.
GenevestigatoriO88502.

Interactioni

Protein-protein interaction databases

BioGridi202082. 1 interaction.
MINTiMINT-4996272.

Structurei

3D structure databases

ProteinModelPortaliO88502.
SMRiO88502. Positions 211-324, 478-812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 28072PASPROSITE-ProRule annotationAdd
BLAST
Domaini283 – 32543PACAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni526 – 807282CatalyticBy similarityAdd
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG282089.
GeneTreeiENSGT00760000118889.
HOVERGENiHBG053544.
InParanoidiO88502.
KOiK18437.
OMAiETTTECV.
OrthoDBiEOG7X3QQM.
PhylomeDBiO88502.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88502-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCAPSIHTS ENRTFSHSDG EDEDVDVDVP GPAPRSIQRW STAPGLVEPQ
60 70 80 90 100
PRDNGASKVS VADVQFGPMR FHQDQLQVLL VFTKEDSQCN GFHRACEKAG
110 120 130 140 150
FKCTVTKEVQ TVLTCFQDKL HDIIIIDHRY PRQMDAETLC RSIRSSKFSE
160 170 180 190 200
NTVIVGVVRR VDKEESSLMP FLAAGFTRRF IENPNVMACY NELLQLACGE
210 220 230 240 250
VRSQLKLRAC NSVFTALEKS QEAIEITSED HIIQYANPAF ESTMGYQSGE
260 270 280 290 300
LIGKELAQVP INEKKGDLLD AINSCVTVDK EWQGVYHTQK KNGDNIQQNV
310 320 330 340 350
KIIPVIGQGG KIRHYVSIIR VCNGNNKVET TTECVQTDSQ TDNQAGKHKD
360 370 380 390 400
RRKHSMDAKA VSSRTSDVSS QRRHSSLARI HSMMIEAPIT KVINIINAAQ
410 420 430 440 450
ENSPVPVTEA LNRVLDILRT TELYSPQFNA QDDPHATDLV GGLMSDGLRR
460 470 480 490 500
FSGNEYILAT KNLPPLSNNL ATPVSLHDVP PRIALAIENE EQWDFDIFEL
510 520 530 540 550
EVATQNRPLI YLGLKTFARF GMCEFLQCSE TTLRSWFQMI ESNYHSSNPY
560 570 580 590 600
HNSTHAADVL HATAYFLSRD KIKETLDRID EVAALIAATV HDVDHPGRTN
610 620 630 640 650
SFLCNAGNQL AVLYNDTAVL ESHHVALAFQ LTLENDQCNI FKQMERNDYR
660 670 680 690 700
TLRQSIIDMV LATEMTKHFE HVNKFINSIN KPLTAQESEE PDRSLEDIKA
710 720 730 740 750
MLKTPESRAL IKRMMIKCAD VSNPCRPLEH CIEWAARISE EYFSQTDEEK
760 770 780 790 800
QLDLPVVMPV FDRNTCSIPK SQISFIDYFI TDMFDAWDAF VDLPNLMQHL
810 820
DDNFRYWKGL DEKKLRSLRP PPE
Length:823
Mass (Da):93,171
Last modified:November 1, 1998 - v1
Checksum:i7FD9BE4BAEB9BCF2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067806 mRNA. Translation: AAC40194.1.
BC125578 mRNA. Translation: AAI25579.1.
BC132145 mRNA. Translation: AAI32146.1.
CCDSiCCDS40005.1.
RefSeqiNP_032829.1. NM_008803.2.
UniGeneiMm.371577.

Genome annotation databases

EnsembliENSMUST00000026672; ENSMUSP00000026672; ENSMUSG00000025584.
GeneIDi18584.
KEGGimmu:18584.
UCSCiuc009ibt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067806 mRNA. Translation: AAC40194.1 .
BC125578 mRNA. Translation: AAI25579.1 .
BC132145 mRNA. Translation: AAI32146.1 .
CCDSi CCDS40005.1.
RefSeqi NP_032829.1. NM_008803.2.
UniGenei Mm.371577.

3D structure databases

ProteinModelPortali O88502.
SMRi O88502. Positions 211-324, 478-812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202082. 1 interaction.
MINTi MINT-4996272.

PTM databases

PhosphoSitei O88502.

Proteomic databases

PaxDbi O88502.
PRIDEi O88502.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026672 ; ENSMUSP00000026672 ; ENSMUSG00000025584 .
GeneIDi 18584.
KEGGi mmu:18584.
UCSCi uc009ibt.1. mouse.

Organism-specific databases

CTDi 5151.
MGIi MGI:1277116. Pde8a.

Phylogenomic databases

eggNOGi NOG282089.
GeneTreei ENSGT00760000118889.
HOVERGENi HBG053544.
InParanoidi O88502.
KOi K18437.
OMAi ETTTECV.
OrthoDBi EOG7X3QQM.
PhylomeDBi O88502.
TreeFami TF314638.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .

Miscellaneous databases

NextBioi 294456.
PROi O88502.
SOURCEi Search...

Gene expression databases

Bgeei O88502.
CleanExi MM_PDE8A.
Genevestigatori O88502.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF13426. PAS_9. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cAMP-specific cyclic nucleotide phosphodiesterase."
    Soderling S.H., Bayuga S.J., Beavo J.A.
    Proc. Natl. Acad. Sci. U.S.A. 95:8991-8996(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-456, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPDE8A_MOUSE
AccessioniPrimary (citable) accession number: O88502
Secondary accession number(s): Q059P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3