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O88502

- PDE8A_MOUSE

UniProt

O88502 - PDE8A_MOUSE

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Protein
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Gene
Pde8a, Pde8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development By similarity.

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Inhibited by dipyridimole. Insensitive to selective PDE inhibitor rolipram and to the non-selective inhibitor, IBMX.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei551 – 5511Proton donor By similarity
Metal bindingi555 – 5551Divalent metal cation 1 By similarity
Metal bindingi591 – 5911Divalent metal cation 1 By similarity
Metal bindingi592 – 5921Divalent metal cation 1 By similarity
Metal bindingi592 – 5921Divalent metal cation 2 By similarity
Metal bindingi720 – 7201Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. phosphorelay response regulator activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (EC:3.1.4.53)
Short name:
MmPDE8
Gene namesi
Name:Pde8a
Synonyms:Pde8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1277116. Pde8a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
PRO_0000198839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei355 – 3551Phosphoserine; by PKA By similarity
Modified residuei452 – 4521Phosphoserine By similarity
Modified residuei456 – 4561Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated at Ser-355 by PKA under elevated cAMP conditions, this enhances catalytic activity By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88502.
PRIDEiO88502.

PTM databases

PhosphoSiteiO88502.

Expressioni

Tissue specificityi

Highest levels in testis > eye > liver > skeletal muscle > heart > 7-day embryo > kidney > ovary > brain. In the testis, expressed specifically in the seminiferous epithelium in a spatial and temporal manner.

Developmental stagei

Levels of expression decrease sometime between embryo day 7 and day 11. In the testis, expression restricted to middle and late pachytene spermatocytes.

Gene expression databases

BgeeiO88502.
CleanExiMM_PDE8A.
GenevestigatoriO88502.

Interactioni

Protein-protein interaction databases

BioGridi202082. 1 interaction.
MINTiMINT-4996272.

Structurei

3D structure databases

ProteinModelPortaliO88502.
SMRiO88502. Positions 478-812.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 28072PAS
Add
BLAST
Domaini283 – 32543PAC
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni526 – 807282Catalytic By similarity
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG282089.
GeneTreeiENSGT00730000110321.
HOVERGENiHBG053544.
InParanoidiQ059P6.
KOiK01120.
OMAiETTTECV.
OrthoDBiEOG7X3QQM.
PhylomeDBiO88502.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88502-1 [UniParc]FASTAAdd to Basket

« Hide

MGCAPSIHTS ENRTFSHSDG EDEDVDVDVP GPAPRSIQRW STAPGLVEPQ    50
PRDNGASKVS VADVQFGPMR FHQDQLQVLL VFTKEDSQCN GFHRACEKAG 100
FKCTVTKEVQ TVLTCFQDKL HDIIIIDHRY PRQMDAETLC RSIRSSKFSE 150
NTVIVGVVRR VDKEESSLMP FLAAGFTRRF IENPNVMACY NELLQLACGE 200
VRSQLKLRAC NSVFTALEKS QEAIEITSED HIIQYANPAF ESTMGYQSGE 250
LIGKELAQVP INEKKGDLLD AINSCVTVDK EWQGVYHTQK KNGDNIQQNV 300
KIIPVIGQGG KIRHYVSIIR VCNGNNKVET TTECVQTDSQ TDNQAGKHKD 350
RRKHSMDAKA VSSRTSDVSS QRRHSSLARI HSMMIEAPIT KVINIINAAQ 400
ENSPVPVTEA LNRVLDILRT TELYSPQFNA QDDPHATDLV GGLMSDGLRR 450
FSGNEYILAT KNLPPLSNNL ATPVSLHDVP PRIALAIENE EQWDFDIFEL 500
EVATQNRPLI YLGLKTFARF GMCEFLQCSE TTLRSWFQMI ESNYHSSNPY 550
HNSTHAADVL HATAYFLSRD KIKETLDRID EVAALIAATV HDVDHPGRTN 600
SFLCNAGNQL AVLYNDTAVL ESHHVALAFQ LTLENDQCNI FKQMERNDYR 650
TLRQSIIDMV LATEMTKHFE HVNKFINSIN KPLTAQESEE PDRSLEDIKA 700
MLKTPESRAL IKRMMIKCAD VSNPCRPLEH CIEWAARISE EYFSQTDEEK 750
QLDLPVVMPV FDRNTCSIPK SQISFIDYFI TDMFDAWDAF VDLPNLMQHL 800
DDNFRYWKGL DEKKLRSLRP PPE 823
Length:823
Mass (Da):93,171
Last modified:November 1, 1998 - v1
Checksum:i7FD9BE4BAEB9BCF2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067806 mRNA. Translation: AAC40194.1.
BC125578 mRNA. Translation: AAI25579.1.
BC132145 mRNA. Translation: AAI32146.1.
CCDSiCCDS40005.1.
RefSeqiNP_032829.1. NM_008803.2.
UniGeneiMm.371577.

Genome annotation databases

EnsembliENSMUST00000026672; ENSMUSP00000026672; ENSMUSG00000025584.
GeneIDi18584.
KEGGimmu:18584.
UCSCiuc009ibt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067806 mRNA. Translation: AAC40194.1 .
BC125578 mRNA. Translation: AAI25579.1 .
BC132145 mRNA. Translation: AAI32146.1 .
CCDSi CCDS40005.1.
RefSeqi NP_032829.1. NM_008803.2.
UniGenei Mm.371577.

3D structure databases

ProteinModelPortali O88502.
SMRi O88502. Positions 478-812.
ModBasei Search...

Protein-protein interaction databases

BioGridi 202082. 1 interaction.
MINTi MINT-4996272.

PTM databases

PhosphoSitei O88502.

Proteomic databases

PaxDbi O88502.
PRIDEi O88502.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026672 ; ENSMUSP00000026672 ; ENSMUSG00000025584 .
GeneIDi 18584.
KEGGi mmu:18584.
UCSCi uc009ibt.1. mouse.

Organism-specific databases

CTDi 5151.
MGIi MGI:1277116. Pde8a.

Phylogenomic databases

eggNOGi NOG282089.
GeneTreei ENSGT00730000110321.
HOVERGENi HBG053544.
InParanoidi Q059P6.
KOi K01120.
OMAi ETTTECV.
OrthoDBi EOG7X3QQM.
PhylomeDBi O88502.
TreeFami TF314638.

Enzyme and pathway databases

UniPathwayi UPA00762 ; UER00747 .

Miscellaneous databases

NextBioi 294456.
PROi O88502.
SOURCEi Search...

Gene expression databases

Bgeei O88502.
CleanExi MM_PDE8A.
Genevestigatori O88502.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR000014. PAS.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF13426. PAS_9. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cAMP-specific cyclic nucleotide phosphodiesterase."
    Soderling S.H., Bayuga S.J., Beavo J.A.
    Proc. Natl. Acad. Sci. U.S.A. 95:8991-8996(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-456, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPDE8A_MOUSE
AccessioniPrimary (citable) accession number: O88502
Secondary accession number(s): Q059P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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