Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O88502

- PDE8A_MOUSE

UniProt

O88502 - PDE8A_MOUSE

Protein

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Gene

Pde8a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development By similarity.By similarity

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by dipyridimole. Insensitive to selective PDE inhibitor rolipram and to the non-selective inhibitor, IBMX.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei551 – 5511Proton donorBy similarity
    Metal bindingi555 – 5551Divalent metal cation 1By similarity
    Metal bindingi591 – 5911Divalent metal cation 1By similarity
    Metal bindingi592 – 5921Divalent metal cation 1By similarity
    Metal bindingi592 – 5921Divalent metal cation 2By similarity
    Metal bindingi720 – 7201Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. phosphorelay response regulator activity Source: InterPro

    GO - Biological processi

    1. cAMP catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00762; UER00747.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (EC:3.1.4.53)
    Short name:
    MmPDE8
    Gene namesi
    Name:Pde8a
    Synonyms:Pde8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1277116. Pde8a.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 823823High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8APRO_0000198839Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei355 – 3551Phosphoserine; by PKABy similarity
    Modified residuei452 – 4521PhosphoserineBy similarity
    Modified residuei456 – 4561Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylated at Ser-355 by PKA under elevated cAMP conditions, this enhances catalytic activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO88502.
    PRIDEiO88502.

    PTM databases

    PhosphoSiteiO88502.

    Expressioni

    Tissue specificityi

    Highest levels in testis > eye > liver > skeletal muscle > heart > 7-day embryo > kidney > ovary > brain. In the testis, expressed specifically in the seminiferous epithelium in a spatial and temporal manner.

    Developmental stagei

    Levels of expression decrease sometime between embryo day 7 and day 11. In the testis, expression restricted to middle and late pachytene spermatocytes.

    Gene expression databases

    BgeeiO88502.
    CleanExiMM_PDE8A.
    GenevestigatoriO88502.

    Interactioni

    Protein-protein interaction databases

    BioGridi202082. 1 interaction.
    MINTiMINT-4996272.

    Structurei

    3D structure databases

    ProteinModelPortaliO88502.
    SMRiO88502. Positions 478-812.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini209 – 28072PASPROSITE-ProRule annotationAdd
    BLAST
    Domaini283 – 32543PACAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni526 – 807282CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

    Sequence similaritiesi

    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG282089.
    GeneTreeiENSGT00730000110321.
    HOVERGENiHBG053544.
    InParanoidiQ059P6.
    KOiK01120.
    OMAiETTTECV.
    OrthoDBiEOG7X3QQM.
    PhylomeDBiO88502.
    TreeFamiTF314638.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR000014. PAS.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF13426. PAS_9. 1 hit.
    PF00233. PDEase_I. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50112. PAS. 1 hit.
    PS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O88502-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCAPSIHTS ENRTFSHSDG EDEDVDVDVP GPAPRSIQRW STAPGLVEPQ    50
    PRDNGASKVS VADVQFGPMR FHQDQLQVLL VFTKEDSQCN GFHRACEKAG 100
    FKCTVTKEVQ TVLTCFQDKL HDIIIIDHRY PRQMDAETLC RSIRSSKFSE 150
    NTVIVGVVRR VDKEESSLMP FLAAGFTRRF IENPNVMACY NELLQLACGE 200
    VRSQLKLRAC NSVFTALEKS QEAIEITSED HIIQYANPAF ESTMGYQSGE 250
    LIGKELAQVP INEKKGDLLD AINSCVTVDK EWQGVYHTQK KNGDNIQQNV 300
    KIIPVIGQGG KIRHYVSIIR VCNGNNKVET TTECVQTDSQ TDNQAGKHKD 350
    RRKHSMDAKA VSSRTSDVSS QRRHSSLARI HSMMIEAPIT KVINIINAAQ 400
    ENSPVPVTEA LNRVLDILRT TELYSPQFNA QDDPHATDLV GGLMSDGLRR 450
    FSGNEYILAT KNLPPLSNNL ATPVSLHDVP PRIALAIENE EQWDFDIFEL 500
    EVATQNRPLI YLGLKTFARF GMCEFLQCSE TTLRSWFQMI ESNYHSSNPY 550
    HNSTHAADVL HATAYFLSRD KIKETLDRID EVAALIAATV HDVDHPGRTN 600
    SFLCNAGNQL AVLYNDTAVL ESHHVALAFQ LTLENDQCNI FKQMERNDYR 650
    TLRQSIIDMV LATEMTKHFE HVNKFINSIN KPLTAQESEE PDRSLEDIKA 700
    MLKTPESRAL IKRMMIKCAD VSNPCRPLEH CIEWAARISE EYFSQTDEEK 750
    QLDLPVVMPV FDRNTCSIPK SQISFIDYFI TDMFDAWDAF VDLPNLMQHL 800
    DDNFRYWKGL DEKKLRSLRP PPE 823
    Length:823
    Mass (Da):93,171
    Last modified:November 1, 1998 - v1
    Checksum:i7FD9BE4BAEB9BCF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067806 mRNA. Translation: AAC40194.1.
    BC125578 mRNA. Translation: AAI25579.1.
    BC132145 mRNA. Translation: AAI32146.1.
    CCDSiCCDS40005.1.
    RefSeqiNP_032829.1. NM_008803.2.
    UniGeneiMm.371577.

    Genome annotation databases

    EnsembliENSMUST00000026672; ENSMUSP00000026672; ENSMUSG00000025584.
    GeneIDi18584.
    KEGGimmu:18584.
    UCSCiuc009ibt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067806 mRNA. Translation: AAC40194.1 .
    BC125578 mRNA. Translation: AAI25579.1 .
    BC132145 mRNA. Translation: AAI32146.1 .
    CCDSi CCDS40005.1.
    RefSeqi NP_032829.1. NM_008803.2.
    UniGenei Mm.371577.

    3D structure databases

    ProteinModelPortali O88502.
    SMRi O88502. Positions 478-812.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202082. 1 interaction.
    MINTi MINT-4996272.

    PTM databases

    PhosphoSitei O88502.

    Proteomic databases

    PaxDbi O88502.
    PRIDEi O88502.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026672 ; ENSMUSP00000026672 ; ENSMUSG00000025584 .
    GeneIDi 18584.
    KEGGi mmu:18584.
    UCSCi uc009ibt.1. mouse.

    Organism-specific databases

    CTDi 5151.
    MGIi MGI:1277116. Pde8a.

    Phylogenomic databases

    eggNOGi NOG282089.
    GeneTreei ENSGT00730000110321.
    HOVERGENi HBG053544.
    InParanoidi Q059P6.
    KOi K01120.
    OMAi ETTTECV.
    OrthoDBi EOG7X3QQM.
    PhylomeDBi O88502.
    TreeFami TF314638.

    Enzyme and pathway databases

    UniPathwayi UPA00762 ; UER00747 .

    Miscellaneous databases

    NextBioi 294456.
    PROi O88502.
    SOURCEi Search...

    Gene expression databases

    Bgeei O88502.
    CleanExi MM_PDE8A.
    Genevestigatori O88502.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR000014. PAS.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF13426. PAS_9. 1 hit.
    PF00233. PDEase_I. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50112. PAS. 1 hit.
    PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a cAMP-specific cyclic nucleotide phosphodiesterase."
      Soderling S.H., Bayuga S.J., Beavo J.A.
      Proc. Natl. Acad. Sci. U.S.A. 95:8991-8996(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-456, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPDE8A_MOUSE
    AccessioniPrimary (citable) accession number: O88502
    Secondary accession number(s): Q059P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3