High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

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O88502 (PDE8A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Short name=MmPDE8
EC=3.1.4.17

Gene names

Name:	Pde8a
Synonyms:	Pde8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	823 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development By similarity.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H₂O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Inhibited by dipyridimole. Insensitive to selective PDE inhibitor rolipram and to the non-selective inhibitor, IBMX.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Tissue specificity	Highest levels in testis > eye > liver > skeletal muscle > heart > 7-day embryo > kidney > ovary > brain. In the testis, expressed specifically in the seminiferous epithelium in a spatial and temporal manner.
Developmental stage	Levels of expression decrease sometime between embryo day 7 and day 11. In the testis, expression restricted to middle and late pachytene spermatocytes.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from direct assay Ref.1. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 823

823

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

PRO_0000198839

Regions

Domain

209 – 280

PAS

Domain

283 – 325

PAC

Region

526 – 807

282

Catalytic By similarity

Sites

Active site

551

Proton donor By similarity

Metal binding

555

Divalent metal cation 1 By similarity

Metal binding

591

Divalent metal cation 1 By similarity

Metal binding

592

Divalent metal cation 1 By similarity

Metal binding

592

Divalent metal cation 2 By similarity

Metal binding

720

Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue

452

Phosphoserine Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

O88502 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7FD9BE4BAEB9BCF2
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FASTA

823

93,171

        10         20         30         40         50         60 
MGCAPSIHTS ENRTFSHSDG EDEDVDVDVP GPAPRSIQRW STAPGLVEPQ PRDNGASKVS 

        70         80         90        100        110        120 
VADVQFGPMR FHQDQLQVLL VFTKEDSQCN GFHRACEKAG FKCTVTKEVQ TVLTCFQDKL 

       130        140        150        160        170        180 
HDIIIIDHRY PRQMDAETLC RSIRSSKFSE NTVIVGVVRR VDKEESSLMP FLAAGFTRRF 

       190        200        210        220        230        240 
IENPNVMACY NELLQLACGE VRSQLKLRAC NSVFTALEKS QEAIEITSED HIIQYANPAF 

       250        260        270        280        290        300 
ESTMGYQSGE LIGKELAQVP INEKKGDLLD AINSCVTVDK EWQGVYHTQK KNGDNIQQNV 

       310        320        330        340        350        360 
KIIPVIGQGG KIRHYVSIIR VCNGNNKVET TTECVQTDSQ TDNQAGKHKD RRKHSMDAKA 

       370        380        390        400        410        420 
VSSRTSDVSS QRRHSSLARI HSMMIEAPIT KVINIINAAQ ENSPVPVTEA LNRVLDILRT 

       430        440        450        460        470        480 
TELYSPQFNA QDDPHATDLV GGLMSDGLRR FSGNEYILAT KNLPPLSNNL ATPVSLHDVP 

       490        500        510        520        530        540 
PRIALAIENE EQWDFDIFEL EVATQNRPLI YLGLKTFARF GMCEFLQCSE TTLRSWFQMI 

       550        560        570        580        590        600 
ESNYHSSNPY HNSTHAADVL HATAYFLSRD KIKETLDRID EVAALIAATV HDVDHPGRTN 

       610        620        630        640        650        660 
SFLCNAGNQL AVLYNDTAVL ESHHVALAFQ LTLENDQCNI FKQMERNDYR TLRQSIIDMV 

       670        680        690        700        710        720 
LATEMTKHFE HVNKFINSIN KPLTAQESEE PDRSLEDIKA MLKTPESRAL IKRMMIKCAD 

       730        740        750        760        770        780 
VSNPCRPLEH CIEWAARISE EYFSQTDEEK QLDLPVVMPV FDRNTCSIPK SQISFIDYFI 

       790        800        810        820 
TDMFDAWDAF VDLPNLMQHL DDNFRYWKGL DEKKLRSLRP PPE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a cAMP-specific cyclic nucleotide phosphodiesterase." Soderling S.H., Bayuga S.J., Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 95:8991-8996(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF067806 mRNA. Translation: AAC40194.1. BC125578 mRNA. Translation: AAI25579.1. BC132145 mRNA. Translation: AAI32146.1.
IPI	IPI00986106.
RefSeq	NP_032829.1. NM_008803.2.
UniGene	Mm.371577.
3D structure databases
ProteinModelPortal	O88502.
SMR	O88502. Positions 211-324, 478-812.
ModBase	Search...
PTM databases
PhosphoSite	O88502.
Proteomic databases
PaxDb	O88502.
PRIDE	O88502.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026672; ENSMUSP00000026672; ENSMUSG00000025584.
GeneID	18584.
KEGG	mmu:18584.
UCSC	uc009ibt.1. mouse.
Organism-specific databases
CTD	5151.
MGI	MGI:1277116. Pde8a.
Phylogenomic databases
eggNOG	NOG282089.
GeneTree	ENSGT00690000101749.
HOVERGEN	HBG053544.
InParanoid	Q059P6.
KO	K01120.
OMA	ETTTECV.
OrthoDB	EOG4SBDX7.
Enzyme and pathway databases
UniPathway	UPA00762; UER00747.
Gene expression databases
ArrayExpress	O88502.
Bgee	O88502.
CleanEx	MM_PDE8A.
Genevestigator	O88502.
GermOnline	ENSMUSG00000025584. Mus musculus.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR000014. PAS. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50113. PAC. False negative. PS50112. PAS. 1 hit. PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294456.
SOURCE	Search...

Entry information

Entry name

PDE8A_MOUSE

Accession

Primary (citable) accession number: O88502
Secondary accession number(s): Q059P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents