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Protein

Bcl-2-like protein 11

Gene

Bcl2l11

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis and anoikis.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-RNO-111446. Activation of BIM and translocation to mitochondria.
R-RNO-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-RNO-193648. NRAGE signals death through JNK.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-like protein 11
Short name:
Bcl2-L-11
Alternative name(s):
Bcl-2-related ovarian death protein
Bcl2-interacting mediator of cell death
Gene namesi
Name:Bcl2l11
Synonyms:Bim, Bod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi628774. Bcl2l11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic dynein complex Source: Ensembl
  • extrinsic component of membrane Source: Ensembl
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000028141 – 196Bcl-2-like protein 11Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65Phosphoserine; by MAPKBy similarity1
Modified residuei73PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei90PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-65 by MAPK1/MAPK3 leads interaction with TRIM2 and ubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein.By similarity
Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation (PubMed:21478148). Conversely, deubiquitination catalyzed by USP27X stabilizes the protein (By similarity).By similarity1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88498.
PRIDEiO88498.

PTM databases

iPTMnetiO88498.
PhosphoSitePlusiO88498.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSRNOG00000016551.
GenevisibleiO88498. RN.

Interactioni

Subunit structurei

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins, including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BCL2L2/BCLW. Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK (PubMed:9731710). Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L); BH3 integrity is required for BCL2L1-binding. Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation (PubMed:21478148). Interacts (via BH3) with MCL1; this interaction may sequester BCL2L11 and prevent its pro-apoptotic activity (PubMed:9731710). When phosphorylated, isoform BimEL interacts with USP27X; this interaction leads to BCL2L11 deubiquitination and stabilization (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249128. 4 interactors.
IntActiO88498. 5 interactors.
STRINGi10116.ENSRNOP00000039006.

Structurei

3D structure databases

ProteinModelPortaliO88498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 160BH3Add BLAST15

Domaini

The BH3 motif is required for the interaction with Bcl-2 proteins and cytotoxicity.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
InParanoidiO88498.
KOiK16341.
OMAiAEDHPQM.
OrthoDBiEOG091G0XD3.
PhylomeDBiO88498.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform BOD-L (identifier: O88498-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKQPSDVNS ECDREGGQLQ PAERPPQLRP GAPTSLQTES QGNPDGEGDR
60 70 80 90 100
CPHGSPQGPL APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS
110 120 130 140 150
PAPMSCDKST QTPSPPCQAF NHYLSAMASI RQSQEEPEDL RPEIRIAQEL
160 170 180 190
RRIGDEFNET YTRRAFANDY REAEDHPQMV ILQLLRFIFR LVWRRH
Length:196
Mass (Da):22,056
Last modified:November 1, 1998 - v1
Checksum:iB4D2146F9C0B37A0
GO
Isoform BimL (identifier: O88498-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Show »
Length:140
Mass (Da):16,194
Checksum:iCEEF7D3EC2E38BC8
GO
Isoform BOD-M (identifier: O88498-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Show »
Length:110
Mass (Da):12,943
Checksum:i026E3C4478F42EEE
GO
Isoform BOD-S (identifier: O88498-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: Produced by alternative initiation at Met-104 of isoform BOD-L.
Show »
Length:93
Mass (Da):11,154
Checksum:i52C58F775B96F0BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136E → D in AAC23594 (PubMed:9731710).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186681 – 103Missing in isoform BOD-S. CuratedAdd BLAST103
Alternative sequenceiVSP_00053942 – 127Missing in isoform BOD-M. CuratedAdd BLAST86
Alternative sequenceiVSP_00053842 – 97Missing in isoform BimL. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065433 mRNA. Translation: AAC23595.1.
AF065431 mRNA. Translation: AAC23593.1.
AF065432 mRNA. Translation: AAC23594.1.
AF136927 mRNA. Translation: AAD26594.1.
RefSeqiNP_072134.1. NM_022612.1. [O88498-2]
NP_741985.1. NM_171988.2. [O88498-1]
NP_741986.1. NM_171989.1.
UniGeneiRn.82709.

Genome annotation databases

EnsembliENSRNOT00000022596; ENSRNOP00000022596; ENSRNOG00000016551. [O88498-2]
ENSRNOT00000051069; ENSRNOP00000039006; ENSRNOG00000016551. [O88498-1]
GeneIDi64547.
KEGGirno:64547.
UCSCiRGD:628774. rat. [O88498-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065433 mRNA. Translation: AAC23595.1.
AF065431 mRNA. Translation: AAC23593.1.
AF065432 mRNA. Translation: AAC23594.1.
AF136927 mRNA. Translation: AAD26594.1.
RefSeqiNP_072134.1. NM_022612.1. [O88498-2]
NP_741985.1. NM_171988.2. [O88498-1]
NP_741986.1. NM_171989.1.
UniGeneiRn.82709.

3D structure databases

ProteinModelPortaliO88498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249128. 4 interactors.
IntActiO88498. 5 interactors.
STRINGi10116.ENSRNOP00000039006.

PTM databases

iPTMnetiO88498.
PhosphoSitePlusiO88498.

Proteomic databases

PaxDbiO88498.
PRIDEiO88498.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022596; ENSRNOP00000022596; ENSRNOG00000016551. [O88498-2]
ENSRNOT00000051069; ENSRNOP00000039006; ENSRNOG00000016551. [O88498-1]
GeneIDi64547.
KEGGirno:64547.
UCSCiRGD:628774. rat. [O88498-1]

Organism-specific databases

CTDi10018.
RGDi628774. Bcl2l11.

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
InParanoidiO88498.
KOiK16341.
OMAiAEDHPQM.
OrthoDBiEOG091G0XD3.
PhylomeDBiO88498.

Enzyme and pathway databases

ReactomeiR-RNO-111446. Activation of BIM and translocation to mitochondria.
R-RNO-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-RNO-193648. NRAGE signals death through JNK.

Miscellaneous databases

PROiO88498.

Gene expression databases

BgeeiENSRNOG00000016551.
GenevisibleiO88498. RN.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB2L11_RAT
AccessioniPrimary (citable) accession number: O88498
Secondary accession number(s): O88497, Q9WUI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.