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Protein

Bcl-2-like protein 11

Gene

Bcl2l11

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis and anoikis.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-RNO-111446. Activation of BIM and translocation to mitochondria.
R-RNO-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-RNO-193648. NRAGE signals death through JNK.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-like protein 11
Short name:
Bcl2-L-11
Alternative name(s):
Bcl-2-related ovarian death protein
Bcl2-interacting mediator of cell death
Gene namesi
Name:Bcl2l11
Synonyms:Bim, Bod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi628774. Bcl2l11.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Bcl-2-like protein 11PRO_0000002814Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine; by MAPKBy similarity
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei83 – 831PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation at Ser-65 by MAPK1/MAPK3 induces interaction with TRIM2, followed by ubiquitination.By similarity
Ubiquitinated by TRIM2 following phosphorylation by MAPK1/MAPK3, leading to degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88498.
PRIDEiO88498.

PTM databases

iPTMnetiO88498.
PhosphoSiteiO88498.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

GenevisibleiO88498. RN.

Interactioni

Subunit structurei

Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK. Interacts with DYNLL1 and YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation. Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity.2 Publications

Protein-protein interaction databases

BioGridi249128. 4 interactions.
IntActiO88498. 5 interactions.
STRINGi10116.ENSRNOP00000039006.

Structurei

3D structure databases

ProteinModelPortaliO88498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 16015BH3Add
BLAST

Domaini

The BH3 motif is required for Bcl-2 binding and cytotoxicity.

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
InParanoidiO88498.
KOiK16341.
OMAiIRLVWRM.
OrthoDBiEOG7X9G87.
PhylomeDBiO88498.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform BOD-L (identifier: O88498-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKQPSDVNS ECDREGGQLQ PAERPPQLRP GAPTSLQTES QGNPDGEGDR
60 70 80 90 100
CPHGSPQGPL APPASPGPFA TRSPLFIFVR RSSLLSRSSS GYFSFDTDRS
110 120 130 140 150
PAPMSCDKST QTPSPPCQAF NHYLSAMASI RQSQEEPEDL RPEIRIAQEL
160 170 180 190
RRIGDEFNET YTRRAFANDY REAEDHPQMV ILQLLRFIFR LVWRRH
Length:196
Mass (Da):22,056
Last modified:November 1, 1998 - v1
Checksum:iB4D2146F9C0B37A0
GO
Isoform BimL (identifier: O88498-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-97: Missing.

Show »
Length:140
Mass (Da):16,194
Checksum:iCEEF7D3EC2E38BC8
GO
Isoform BOD-M (identifier: O88498-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-127: Missing.

Show »
Length:110
Mass (Da):12,943
Checksum:i026E3C4478F42EEE
GO
Isoform BOD-S (identifier: O88498-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: Produced by alternative initiation at Met-104 of isoform BOD-L.
Show »
Length:93
Mass (Da):11,154
Checksum:i52C58F775B96F0BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361E → D in AAC23594 (PubMed:9731710).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform BOD-S. CuratedVSP_018668Add
BLAST
Alternative sequencei42 – 12786Missing in isoform BOD-M. CuratedVSP_000539Add
BLAST
Alternative sequencei42 – 9756Missing in isoform BimL. 1 PublicationVSP_000538Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065433 mRNA. Translation: AAC23595.1.
AF065431 mRNA. Translation: AAC23593.1.
AF065432 mRNA. Translation: AAC23594.1.
AF136927 mRNA. Translation: AAD26594.1.
RefSeqiNP_072134.1. NM_022612.1. [O88498-2]
NP_741985.1. NM_171988.2. [O88498-1]
NP_741986.1. NM_171989.1.
UniGeneiRn.82709.

Genome annotation databases

EnsembliENSRNOT00000022596; ENSRNOP00000022596; ENSRNOG00000016551. [O88498-2]
ENSRNOT00000051069; ENSRNOP00000039006; ENSRNOG00000016551. [O88498-1]
GeneIDi64547.
KEGGirno:64547.
UCSCiRGD:628774. rat. [O88498-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065433 mRNA. Translation: AAC23595.1.
AF065431 mRNA. Translation: AAC23593.1.
AF065432 mRNA. Translation: AAC23594.1.
AF136927 mRNA. Translation: AAD26594.1.
RefSeqiNP_072134.1. NM_022612.1. [O88498-2]
NP_741985.1. NM_171988.2. [O88498-1]
NP_741986.1. NM_171989.1.
UniGeneiRn.82709.

3D structure databases

ProteinModelPortaliO88498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249128. 4 interactions.
IntActiO88498. 5 interactions.
STRINGi10116.ENSRNOP00000039006.

PTM databases

iPTMnetiO88498.
PhosphoSiteiO88498.

Proteomic databases

PaxDbiO88498.
PRIDEiO88498.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022596; ENSRNOP00000022596; ENSRNOG00000016551. [O88498-2]
ENSRNOT00000051069; ENSRNOP00000039006; ENSRNOG00000016551. [O88498-1]
GeneIDi64547.
KEGGirno:64547.
UCSCiRGD:628774. rat. [O88498-1]

Organism-specific databases

CTDi10018.
RGDi628774. Bcl2l11.

Phylogenomic databases

eggNOGiENOG410IZKS. Eukaryota.
ENOG410Y8GB. LUCA.
GeneTreeiENSGT00390000003178.
InParanoidiO88498.
KOiK16341.
OMAiIRLVWRM.
OrthoDBiEOG7X9G87.
PhylomeDBiO88498.

Enzyme and pathway databases

ReactomeiR-RNO-111446. Activation of BIM and translocation to mitochondria.
R-RNO-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-RNO-193648. NRAGE signals death through JNK.

Miscellaneous databases

NextBioi613424.
PROiO88498.

Gene expression databases

GenevisibleiO88498. RN.

Family and domain databases

InterProiIPR014771. Apoptosis_Bim_N.
IPR017288. Bcl-2-like_11.
IPR015040. Bcl-x_interacting_BH3_dom.
[Graphical view]
PfamiPF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]
PIRSFiPIRSF037827. Bcl-2-like_p11. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members."
    Hsu S.Y., Lin P., Hsueh A.J.W.
    Mol. Endocrinol. 12:1432-1440(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY (ISOFORMS BOD-L; BOD-M AND BOD-S).
    Tissue: Ovary.
  2. "Cloning of rat bimEL and bimL, and their differential expression in ischemia and normal rat brain."
    Chen D., Simon R.P., Chen J.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BIML).
  3. "Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection."
    Thompson S., Pearson A.N., Ashley M.D., Jessick V., Murphy B.M., Gafken P., Henshall D.C., Morris K.T., Simon R.P., Meller R.
    J. Biol. Chem. 286:19331-19339(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLL1; TRIM2 AND YWHAZ, UBIQUITINATION.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiB2L11_RAT
AccessioniPrimary (citable) accession number: O88498
Secondary accession number(s): O88497, Q9WUI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.