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Protein

Cytoplasmic dynein 1 intermediate chain 2

Gene

Dync1i2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).By similarity

GO - Molecular functioni

  1. motor activity Source: UniProtKB-KW

GO - Biological processi

  1. microtubule-based movement Source: InterPro
  2. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_272448. Recruitment of mitotic centrosome proteins and complexes.
REACT_276302. Loss of Nlp from mitotic centrosomes.
REACT_286537. Loss of proteins required for interphase microtubule organization from the centrosome.
REACT_316383. MHC class II antigen presentation.
REACT_328862. Anchoring of the basal body to the plasma membrane.
REACT_345278. Regulation of PLK1 Activity at G2/M Transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 2
Alternative name(s):
Cytoplasmic dynein intermediate chain 2
Dynein intermediate chain 2, cytosolic
Short name:
DH IC-2
Gene namesi
Name:Dync1i2
Synonyms:Dnci2, Dncic2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107750. Dync1i2.

Subcellular locationi

  1. Cytoplasmcytoskeleton By similarity

GO - Cellular componenti

  1. cytoplasmic dynein complex Source: InterPro
  2. microtubule Source: UniProtKB-KW
  3. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 612611Cytoplasmic dynein 1 intermediate chain 2PRO_0000114656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei84 – 841PhosphoserineBy similarity
Modified residuei91 – 911PhosphoserineBy similarity
Modified residuei95 – 951PhosphoserineBy similarity
Modified residuei98 – 981PhosphoserineBy similarity

Post-translational modificationi

The phosphorylation status of Ser-84 appears to be involved in dynactin-dependent target binding.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO88487.
PaxDbiO88487.
PRIDEiO88487.

2D gel databases

REPRODUCTION-2DPAGEO88487.

PTM databases

PhosphoSiteiO88487.

Expressioni

Gene expression databases

BgeeiO88487.
CleanExiMM_DYNC1I2.
ExpressionAtlasiO88487. baseline and differential.
GenevestigatoriO88487.

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199256. 7 interactions.
IntActiO88487. 11 interactions.
MINTiMINT-3088834.

Structurei

3D structure databases

ProteinModelPortaliO88487.
SMRiO88487. Positions 110-138, 307-557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati251 – 30050WD 1Add
BLAST
Repeati304 – 34441WD 2Add
BLAST
Repeati353 – 39442WD 3Add
BLAST
Repeati403 – 44341WD 4Add
BLAST
Repeati448 – 49346WD 5Add
BLAST
Repeati496 – 53641WD 6Add
BLAST
Repeati542 – 58140WD 7Add
BLAST

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG308180.
GeneTreeiENSGT00730000110228.
HOVERGENiHBG004083.
InParanoidiO88487.
KOiK10415.
OrthoDBiEOG7FR7FX.
TreeFamiTF300553.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. Dynein_IC_1/2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAAVSVQEE
60 70 80 90 100
SDLEKKRREA EALLQSMGLT TDSPIVPPPM SPSSKSVSTP SEAGSQDSGD
110 120 130 140 150
GAVGSRRGPI KLGMAKITQV DFPPREIVTY TKETQTPVTA QPKEDEEEED
160 170 180 190 200
DVATPKPPVE PEEEKTLKKD EENDSKAPPH ELTEEEKQQI LHSEEFLSFF
210 220 230 240 250
DHSTRIVERA LSEQINIFFD YSGRDLEDKE GEIQAGAKLS LNRQFFDERW
260 270 280 290 300
SKHRVVSCLD WSSQYPELLV ASYNNNEEAP HEPDGVALVW NMKYKKTTPE
310 320 330 340 350
YVFHCQSAVM SATFAKFHPN LVVGGTYSGQ IVLWDNRSNK RTPVQRTPLS
360 370 380 390 400
AAAHTHPVYC VNVVGTQNAH NLISISTDGK ICSWSLDMLS HPQDSMELVH
410 420 430 440 450
KQSKAVAVTS MSFPVGDVNN FVVGSEEGSV YTACRHGSKA GISEMFEGHQ
460 470 480 490 500
GPITGIHCHA AVGAVDFSHL FVTSSFDWTV KLWTTKNNKP LYSFEDNSDY
510 520 530 540 550
VYDVMWSPTH PALFACVDGM GRLDLWNLNN DTEVPTASIS VEGNPALNRV
560 570 580 590 600
RWTHSGREIA VGDSEGQIVI YDVGEQIAVP RNDEWARFGR TLAEINANRA
610
DAEEEAATRI PA
Length:612
Mass (Da):68,394
Last modified:November 1, 1998 - v1
Checksum:i1D14CEECF62A5E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063231 mRNA. Translation: AAC33446.1.
AK088015 mRNA. Translation: BAC40097.1.
AK164437 mRNA. Translation: BAE37788.1.
AK168730 mRNA. Translation: BAE40571.1.
CCDSiCCDS16112.1.
RefSeqiNP_001185805.1. NM_001198876.1.
NP_001185806.1. NM_001198877.1.
NP_001185807.1. NM_001198878.1.
NP_034194.1. NM_010064.4.
UniGeneiMm.249479.

Genome annotation databases

EnsembliENSMUST00000081710; ENSMUSP00000080410; ENSMUSG00000027012.
ENSMUST00000112138; ENSMUSP00000107766; ENSMUSG00000027012.
GeneIDi13427.
KEGGimmu:13427.
UCSCiuc008kai.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063231 mRNA. Translation: AAC33446.1.
AK088015 mRNA. Translation: BAC40097.1.
AK164437 mRNA. Translation: BAE37788.1.
AK168730 mRNA. Translation: BAE40571.1.
CCDSiCCDS16112.1.
RefSeqiNP_001185805.1. NM_001198876.1.
NP_001185806.1. NM_001198877.1.
NP_001185807.1. NM_001198878.1.
NP_034194.1. NM_010064.4.
UniGeneiMm.249479.

3D structure databases

ProteinModelPortaliO88487.
SMRiO88487. Positions 110-138, 307-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199256. 7 interactions.
IntActiO88487. 11 interactions.
MINTiMINT-3088834.

PTM databases

PhosphoSiteiO88487.

2D gel databases

REPRODUCTION-2DPAGEO88487.

Proteomic databases

MaxQBiO88487.
PaxDbiO88487.
PRIDEiO88487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081710; ENSMUSP00000080410; ENSMUSG00000027012.
ENSMUST00000112138; ENSMUSP00000107766; ENSMUSG00000027012.
GeneIDi13427.
KEGGimmu:13427.
UCSCiuc008kai.2. mouse.

Organism-specific databases

CTDi1781.
MGIiMGI:107750. Dync1i2.

Phylogenomic databases

eggNOGiNOG308180.
GeneTreeiENSGT00730000110228.
HOVERGENiHBG004083.
InParanoidiO88487.
KOiK10415.
OrthoDBiEOG7FR7FX.
TreeFamiTF300553.

Enzyme and pathway databases

ReactomeiREACT_272448. Recruitment of mitotic centrosome proteins and complexes.
REACT_276302. Loss of Nlp from mitotic centrosomes.
REACT_286537. Loss of proteins required for interphase microtubule organization from the centrosome.
REACT_316383. MHC class II antigen presentation.
REACT_328862. Anchoring of the basal body to the plasma membrane.
REACT_345278. Regulation of PLK1 Activity at G2/M Transition.

Miscellaneous databases

NextBioi283847.
PROiO88487.
SOURCEiSearch...

Gene expression databases

BgeeiO88487.
CleanExiMM_DYNC1I2.
ExpressionAtlasiO88487. baseline and differential.
GenevestigatoriO88487.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. Dynein_IC_1/2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human."
    Crackower M.A., Sinasac D.S., Xia J., Motoyama J., Prochazka M., Rommens J.M., Scherer S.W., Tsui L.-C.
    Genomics 55:257-267(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Eye, Heart and Thymus.
  3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDC1I2_MOUSE
AccessioniPrimary (citable) accession number: O88487
Secondary accession number(s): Q3TGH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: April 1, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.