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O88487

- DC1I2_MOUSE

UniProt

O88487 - DC1I2_MOUSE

Protein

Cytoplasmic dynein 1 intermediate chain 2

Gene

Dync1i2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes By similarity.By similarity

    GO - Molecular functioni

    1. motor activity Source: UniProtKB-KW

    GO - Biological processi

    1. microtubule-based movement Source: InterPro
    2. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic dynein 1 intermediate chain 2
    Alternative name(s):
    Cytoplasmic dynein intermediate chain 2
    Dynein intermediate chain 2, cytosolic
    Short name:
    DH IC-2
    Gene namesi
    Name:Dync1i2
    Synonyms:Dnci2, Dncic2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:107750. Dync1i2.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity

    GO - Cellular componenti

    1. cytoplasmic dynein complex Source: InterPro
    2. microtubule Source: UniProtKB-KW
    3. vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 612611Cytoplasmic dynein 1 intermediate chain 2PRO_0000114656Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei84 – 841PhosphoserineBy similarity
    Modified residuei91 – 911PhosphoserineBy similarity
    Modified residuei95 – 951PhosphoserineBy similarity
    Modified residuei98 – 981PhosphoserineBy similarity

    Post-translational modificationi

    The phosphorylation status of Ser-84 appears to be involved in dynactin-dependent target binding.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO88487.
    PaxDbiO88487.
    PRIDEiO88487.

    2D gel databases

    REPRODUCTION-2DPAGEO88487.

    PTM databases

    PhosphoSiteiO88487.

    Expressioni

    Gene expression databases

    ArrayExpressiO88487.
    BgeeiO88487.
    CleanExiMM_DYNC1I2.
    GenevestigatoriO88487.

    Interactioni

    Subunit structurei

    Homodimer By similarity. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199256. 7 interactions.
    IntActiO88487. 11 interactions.
    MINTiMINT-3088834.

    Structurei

    3D structure databases

    ProteinModelPortaliO88487.
    SMRiO88487. Positions 110-138, 307-557.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati251 – 30050WD 1Add
    BLAST
    Repeati304 – 34441WD 2Add
    BLAST
    Repeati353 – 39442WD 3Add
    BLAST
    Repeati403 – 44341WD 4Add
    BLAST
    Repeati448 – 49346WD 5Add
    BLAST
    Repeati496 – 53641WD 6Add
    BLAST
    Repeati542 – 58140WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the dynein intermediate chain family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG308180.
    GeneTreeiENSGT00730000110228.
    HOVERGENiHBG004083.
    KOiK10415.
    OrthoDBiEOG7FR7FX.
    TreeFamiTF300553.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR025956. Dynein_IC_1/2.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF11540. Dynein_IC2. 1 hit.
    PF00400. WD40. 1 hit.
    [Graphical view]
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88487-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAAVSVQEE    50
    SDLEKKRREA EALLQSMGLT TDSPIVPPPM SPSSKSVSTP SEAGSQDSGD 100
    GAVGSRRGPI KLGMAKITQV DFPPREIVTY TKETQTPVTA QPKEDEEEED 150
    DVATPKPPVE PEEEKTLKKD EENDSKAPPH ELTEEEKQQI LHSEEFLSFF 200
    DHSTRIVERA LSEQINIFFD YSGRDLEDKE GEIQAGAKLS LNRQFFDERW 250
    SKHRVVSCLD WSSQYPELLV ASYNNNEEAP HEPDGVALVW NMKYKKTTPE 300
    YVFHCQSAVM SATFAKFHPN LVVGGTYSGQ IVLWDNRSNK RTPVQRTPLS 350
    AAAHTHPVYC VNVVGTQNAH NLISISTDGK ICSWSLDMLS HPQDSMELVH 400
    KQSKAVAVTS MSFPVGDVNN FVVGSEEGSV YTACRHGSKA GISEMFEGHQ 450
    GPITGIHCHA AVGAVDFSHL FVTSSFDWTV KLWTTKNNKP LYSFEDNSDY 500
    VYDVMWSPTH PALFACVDGM GRLDLWNLNN DTEVPTASIS VEGNPALNRV 550
    RWTHSGREIA VGDSEGQIVI YDVGEQIAVP RNDEWARFGR TLAEINANRA 600
    DAEEEAATRI PA 612
    Length:612
    Mass (Da):68,394
    Last modified:November 1, 1998 - v1
    Checksum:i1D14CEECF62A5E33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063231 mRNA. Translation: AAC33446.1.
    AK088015 mRNA. Translation: BAC40097.1.
    AK164437 mRNA. Translation: BAE37788.1.
    AK168730 mRNA. Translation: BAE40571.1.
    CCDSiCCDS16112.1.
    RefSeqiNP_001185805.1. NM_001198876.1.
    NP_001185806.1. NM_001198877.1.
    NP_001185807.1. NM_001198878.1.
    NP_034194.1. NM_010064.4.
    UniGeneiMm.249479.

    Genome annotation databases

    EnsembliENSMUST00000081710; ENSMUSP00000080410; ENSMUSG00000027012.
    ENSMUST00000112138; ENSMUSP00000107766; ENSMUSG00000027012.
    GeneIDi13427.
    KEGGimmu:13427.
    UCSCiuc008kai.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063231 mRNA. Translation: AAC33446.1 .
    AK088015 mRNA. Translation: BAC40097.1 .
    AK164437 mRNA. Translation: BAE37788.1 .
    AK168730 mRNA. Translation: BAE40571.1 .
    CCDSi CCDS16112.1.
    RefSeqi NP_001185805.1. NM_001198876.1.
    NP_001185806.1. NM_001198877.1.
    NP_001185807.1. NM_001198878.1.
    NP_034194.1. NM_010064.4.
    UniGenei Mm.249479.

    3D structure databases

    ProteinModelPortali O88487.
    SMRi O88487. Positions 110-138, 307-557.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199256. 7 interactions.
    IntActi O88487. 11 interactions.
    MINTi MINT-3088834.

    PTM databases

    PhosphoSitei O88487.

    2D gel databases

    REPRODUCTION-2DPAGE O88487.

    Proteomic databases

    MaxQBi O88487.
    PaxDbi O88487.
    PRIDEi O88487.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081710 ; ENSMUSP00000080410 ; ENSMUSG00000027012 .
    ENSMUST00000112138 ; ENSMUSP00000107766 ; ENSMUSG00000027012 .
    GeneIDi 13427.
    KEGGi mmu:13427.
    UCSCi uc008kai.2. mouse.

    Organism-specific databases

    CTDi 1781.
    MGIi MGI:107750. Dync1i2.

    Phylogenomic databases

    eggNOGi NOG308180.
    GeneTreei ENSGT00730000110228.
    HOVERGENi HBG004083.
    KOi K10415.
    OrthoDBi EOG7FR7FX.
    TreeFami TF300553.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    NextBioi 283847.
    PROi O88487.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88487.
    Bgeei O88487.
    CleanExi MM_DYNC1I2.
    Genevestigatori O88487.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR025956. Dynein_IC_1/2.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF11540. Dynein_IC2. 1 hit.
    PF00400. WD40. 1 hit.
    [Graphical view ]
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human."
      Crackower M.A., Sinasac D.S., Xia J., Motoyama J., Prochazka M., Rommens J.M., Scherer S.W., Tsui L.-C.
      Genomics 55:257-267(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Eye, Heart and Thymus.
    3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiDC1I2_MOUSE
    AccessioniPrimary (citable) accession number: O88487
    Secondary accession number(s): Q3TGH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3