Skip Header

Contribute Send feedback
Read comments (?) or add your own

O88485 (DC1I1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytoplasmic dynein 1 intermediate chain 1
Alternative name(s):
Cytoplasmic dynein intermediate chain 1
Dynein intermediate chain 1, cytosolic
Short name=DH IC-1
Gene names
Name:Dync1i1
Synonyms:Dnci1, Dncic1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length628 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

Subunit structure

Homodimer By similarity. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1H1. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 By similarity. Interacts with DYNLL2. Ref.4

Subcellular location

Cytoplasm By similarity. Chromosome › centromere › kinetochore By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity.

Sequence similarities

Belongs to the dynein intermediate chain family.

Contains 7 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Nr3c1P065372EBI-492834,EBI-492753

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1A (identifier: O88485-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1B (identifier: O88485-2)

The sequence of this isoform differs from the canonical sequence as follows:
     106-125: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 628628Cytoplasmic dynein 1 intermediate chain 1
PRO_0000114653

Regions

Repeat268 – 31750WD 1
Repeat321 – 36141WD 2
Repeat370 – 41142WD 3
Repeat420 – 46041WD 4
Repeat465 – 51046WD 5
Repeat513 – 55341WD 6
Repeat559 – 59840WD 7

Amino acid modifications

Modified residue6181Phosphoserine Ref.5

Natural variations

Alternative sequence106 – 12520Missing in isoform 1B.
VSP_001334

Experimental info

Mutagenesis151 – 1555Missing: Abolishes interaction with DYNLL2. Ref.4
Mutagenesis1531T → A: Impairs interaction with DYNLL2.
Mutagenesis1531T → G: Abolishes interaction with DYNLL2.
Mutagenesis1541Q → A: Abolishes interaction with DYNLL2.
Mutagenesis1551T → G: Abolishes interaction with DYNLL2.
Mutagenesis1551T → S: Impairs interaction with DYNLL2.
Sequence conflict3841Q → H in AAC33444. Ref.1
Sequence conflict3871H → R in AAC33444. Ref.1
Sequence conflict5081L → V in AAC33444. Ref.1
Sequence conflict5111F → S in AAC33444. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1A [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1DFD9264D63B2CE8

FASTA62870,725
        10         20         30         40         50         60 
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS DLDRKRRETE 

        70         80         90        100        110        120 
ALLQSIGISP EPPLVPTPMS PSSKSVSTPS DAGSQDSGDL GPLTRTLQWD TDPSVLQLQS 

       130        140        150        160        170        180 
DSELGRRLHK LGVSKVTQVD FLPREVVSYS KETQTPLATH QSEEDEEDEE MVEPKIGHDS 

       190        200        210        220        230        240 
ELENQEKKQE TKEAPPRELT EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG 

       250        260        270        280        290        300 
RELEEKDGDV QAGANLSFNR QFYDEHWSKH RVVTCMDWSL QYPELMVASY SNNEDAPHEP 

       310        320        330        340        350        360 
DGVALVWNMK FKKTTPEYVF HCQSSVMSVC FARFHPNLVV GGTYSGQIVL WDNRSHRRTP 

       370        380        390        400        410        420 
VQRTPLSAAA HTHPVYCVNV VGTQNAHNLI TVSTDGKMCS WSLDMLSTPQ ESMELVYNKS 

       430        440        450        460        470        480 
KPVAVTGMAF PTGDVNNFVV GSEEGTVYTA CRHGSKAGIG EVFEGHQGPV TGINCHMAVG 

       490        500        510        520        530        540 
PIDFSHLFVT SSFDWTVKLW TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL 

       550        560        570        580        590        600 
DLWNLNSDTE VPTASVAIEG ASALNRVRWA QGGKEVAVGD SEGRIWIYDV GELAVPHNDE 

       610        620 
WTRFARTLVE IRANRADSEE EGAVELAA

« Hide

Isoform 1B [UniParc].

Checksum: 3310615A8823774E
Show »

FASTA60868,510

References

« Hide 'large scale' references
[1]"Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human."
Crackower M.A., Sinasac D.S., Xia J., Motoyama J., Prochazka M., Rommens J.M., Scherer S.W., Tsui L.-C.
Genomics 55:257-267(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B).
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif."
Lo K.W., Naisbitt S., Fan J.S., Sheng M., Zhang M.
J. Biol. Chem. 276:14059-14066(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYNLL2, MUTAGENESIS OF 151-LYS--THR-155.
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-618, MASS SPECTROMETRY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF063229 mRNA. Translation: AAC33444.1.
AF063230 mRNA. Translation: AAC33445.1.
CH466533 Genomic DNA. Translation: EDL13960.1.
BC051992 mRNA. Translation: AAH51992.1.
IPIIPI00131084.
IPI00226946.
RefSeqNP_001177952.1. NM_001191023.1.
NP_001177954.1. NM_001191025.1.
NP_034193.2. NM_010063.4.
UniGeneMm.20893.

3D structure databases

ProteinModelPortalO88485.
SMRO88485. Positions 130-157, 426-590.
ModBaseSearch...

Protein-protein interaction databases

IntActO88485. 2 interactions.
MINTMINT-3088793.

PTM databases

PhosphoSiteO88485.

Proteomic databases

PaxDbO88485.
PRIDEO88485.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000115554; ENSMUSP00000111216; ENSMUSG00000029757.
ENSMUST00000115559; ENSMUSP00000111221; ENSMUSG00000029757.
GeneID13426.
KEGGmmu:13426.

Organism-specific databases

CTD1780.
MGIMGI:107743. Dync1i1.

Phylogenomic databases

eggNOGNOG308180.
GeneTreeENSGT00690000101848.
HOGENOMHOG000116383.
HOVERGENHBG004083.
KOK10415.

Gene expression databases

ArrayExpressO88485.
BgeeO88485.
CleanExMM_DYNC1I1.
GenevestigatorO88485.
GermOnlineENSMUSG00000029757. Mus musculus.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR025956. Dynein_IC_1/2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283843.
SOURCESearch...

Entry information

Entry nameDC1I1_MOUSE
AccessionPrimary (citable) accession number: O88485
Secondary accession number(s): O88486, Q80W09
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents