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Protein

Cytoplasmic dynein 1 intermediate chain 1

Gene

Dync1i1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 1
Alternative name(s):
Cytoplasmic dynein intermediate chain 1
Dynein intermediate chain 1, cytosolic
Short name:
DH IC-1
Gene namesi
Name:Dync1i1
Synonyms:Dnci1, Dncic1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:107743. Dync1i1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Dynein, Kinetochore, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi151 – 1555Missing : Abolishes interaction with DYNLL2. 1 Publication
Mutagenesisi153 – 1531T → A: Impairs interaction with DYNLL2.
Mutagenesisi153 – 1531T → G: Abolishes interaction with DYNLL2.
Mutagenesisi154 – 1541Q → A: Abolishes interaction with DYNLL2.
Mutagenesisi155 – 1551T → G: Abolishes interaction with DYNLL2.
Mutagenesisi155 – 1551T → S: Impairs interaction with DYNLL2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Cytoplasmic dynein 1 intermediate chain 1PRO_0000114653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineCombined sources
Modified residuei94 – 941PhosphoserineCombined sources
Modified residuei97 – 971PhosphoserineCombined sources
Modified residuei159 – 1591PhosphothreonineCombined sources
Modified residuei162 – 1621PhosphoserineCombined sources
Modified residuei180 – 1801PhosphoserineCombined sources
Modified residuei618 – 6181PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88485.
MaxQBiO88485.
PaxDbiO88485.
PRIDEiO88485.

PTM databases

iPTMnetiO88485.
PhosphoSiteiO88485.
SwissPalmiO88485.

Expressioni

Gene expression databases

BgeeiO88485.
CleanExiMM_DYNC1I1.
ExpressionAtlasiO88485. baseline and differential.
GenevisibleiO88485. MM.

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1H1. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with DYNLL2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DazlQ643684EBI-492834,EBI-2024439
Dynll1P631682EBI-492834,EBI-349121
Kif5bQ617683EBI-492834,EBI-776129
Nr3c1P065372EBI-492834,EBI-492753

GO - Molecular functioni

  • dynein light chain binding Source: MGI
  • microtubule binding Source: HGNC
  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi199255. 9 interactions.
DIPiDIP-32048N.
IntActiO88485. 9 interactions.
MINTiMINT-3088793.
STRINGi10090.ENSMUSP00000111221.

Structurei

3D structure databases

ProteinModelPortaliO88485.
SMRiO88485. Positions 130-157, 324-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati268 – 31750WD 1Add
BLAST
Repeati321 – 36141WD 2Add
BLAST
Repeati370 – 41142WD 3Add
BLAST
Repeati420 – 46041WD 4Add
BLAST
Repeati465 – 51046WD 5Add
BLAST
Repeati513 – 55341WD 6Add
BLAST
Repeati559 – 59840WD 7Add
BLAST

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
GeneTreeiENSGT00730000110228.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiO88485.
KOiK10415.
TreeFamiTF300553.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1A (identifier: O88485-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS
60 70 80 90 100
DLDRKRRETE ALLQSIGISP EPPLVPTPMS PSSKSVSTPS DAGSQDSGDL
110 120 130 140 150
GPLTRTLQWD TDPSVLQLQS DSELGRRLHK LGVSKVTQVD FLPREVVSYS
160 170 180 190 200
KETQTPLATH QSEEDEEDEE MVEPKIGHDS ELENQEKKQE TKEAPPRELT
210 220 230 240 250
EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG RELEEKDGDV
260 270 280 290 300
QAGANLSFNR QFYDEHWSKH RVVTCMDWSL QYPELMVASY SNNEDAPHEP
310 320 330 340 350
DGVALVWNMK FKKTTPEYVF HCQSSVMSVC FARFHPNLVV GGTYSGQIVL
360 370 380 390 400
WDNRSHRRTP VQRTPLSAAA HTHPVYCVNV VGTQNAHNLI TVSTDGKMCS
410 420 430 440 450
WSLDMLSTPQ ESMELVYNKS KPVAVTGMAF PTGDVNNFVV GSEEGTVYTA
460 470 480 490 500
CRHGSKAGIG EVFEGHQGPV TGINCHMAVG PIDFSHLFVT SSFDWTVKLW
510 520 530 540 550
TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL DLWNLNSDTE
560 570 580 590 600
VPTASVAIEG ASALNRVRWA QGGKEVAVGD SEGRIWIYDV GELAVPHNDE
610 620
WTRFARTLVE IRANRADSEE EGAVELAA
Length:628
Mass (Da):70,725
Last modified:July 27, 2011 - v2
Checksum:i1DFD9264D63B2CE8
GO
Isoform 1B (identifier: O88485-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     106-125: Missing.

Show »
Length:608
Mass (Da):68,510
Checksum:i3310615A8823774E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841Q → H in AAC33444 (PubMed:10049579).Curated
Sequence conflicti387 – 3871H → R in AAC33444 (PubMed:10049579).Curated
Sequence conflicti508 – 5081L → V in AAC33444 (PubMed:10049579).Curated
Sequence conflicti511 – 5111F → S in AAC33444 (PubMed:10049579).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei106 – 12520Missing in isoform 1B. 1 PublicationVSP_001334Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063229 mRNA. Translation: AAC33444.1.
AF063230 mRNA. Translation: AAC33445.1.
CH466533 Genomic DNA. Translation: EDL13960.1.
BC051992 mRNA. Translation: AAH51992.1.
CCDSiCCDS19903.1. [O88485-1]
CCDS57408.1. [O88485-2]
RefSeqiNP_001177952.1. NM_001191023.1.
NP_001177954.1. NM_001191025.1. [O88485-2]
NP_034193.2. NM_010063.4. [O88485-1]
UniGeneiMm.20893.

Genome annotation databases

EnsembliENSMUST00000115554; ENSMUSP00000111216; ENSMUSG00000029757. [O88485-2]
ENSMUST00000115559; ENSMUSP00000111221; ENSMUSG00000029757. [O88485-1]
GeneIDi13426.
KEGGimmu:13426.
UCSCiuc009awm.2. mouse. [O88485-2]
uc012ehw.1. mouse. [O88485-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063229 mRNA. Translation: AAC33444.1.
AF063230 mRNA. Translation: AAC33445.1.
CH466533 Genomic DNA. Translation: EDL13960.1.
BC051992 mRNA. Translation: AAH51992.1.
CCDSiCCDS19903.1. [O88485-1]
CCDS57408.1. [O88485-2]
RefSeqiNP_001177952.1. NM_001191023.1.
NP_001177954.1. NM_001191025.1. [O88485-2]
NP_034193.2. NM_010063.4. [O88485-1]
UniGeneiMm.20893.

3D structure databases

ProteinModelPortaliO88485.
SMRiO88485. Positions 130-157, 324-592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199255. 9 interactions.
DIPiDIP-32048N.
IntActiO88485. 9 interactions.
MINTiMINT-3088793.
STRINGi10090.ENSMUSP00000111221.

PTM databases

iPTMnetiO88485.
PhosphoSiteiO88485.
SwissPalmiO88485.

Proteomic databases

EPDiO88485.
MaxQBiO88485.
PaxDbiO88485.
PRIDEiO88485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115554; ENSMUSP00000111216; ENSMUSG00000029757. [O88485-2]
ENSMUST00000115559; ENSMUSP00000111221; ENSMUSG00000029757. [O88485-1]
GeneIDi13426.
KEGGimmu:13426.
UCSCiuc009awm.2. mouse. [O88485-2]
uc012ehw.1. mouse. [O88485-1]

Organism-specific databases

CTDi1780.
MGIiMGI:107743. Dync1i1.

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
GeneTreeiENSGT00730000110228.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiO88485.
KOiK10415.
TreeFamiTF300553.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiO88485.
SOURCEiSearch...

Gene expression databases

BgeeiO88485.
CleanExiMM_DYNC1I1.
ExpressionAtlasiO88485. baseline and differential.
GenevisibleiO88485. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human."
    Crackower M.A., Sinasac D.S., Xia J., Motoyama J., Prochazka M., Rommens J.M., Scherer S.W., Tsui L.-C.
    Genomics 55:257-267(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B).
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif."
    Lo K.W., Naisbitt S., Fan J.S., Sheng M., Zhang M.
    J. Biol. Chem. 276:14059-14066(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLL2, MUTAGENESIS OF 151-LYS--THR-155.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; SER-94; SER-97; THR-159; SER-162; SER-180 AND SER-618, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiDC1I1_MOUSE
AccessioniPrimary (citable) accession number: O88485
Secondary accession number(s): O88486, Q80W09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.