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Protein

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial

Gene

Pdp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.

Catalytic activityi

[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi142 – 1421Manganese 1By similarity
Metal bindingi142 – 1421Manganese 2By similarity
Metal bindingi143 – 1431Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi413 – 4131Manganese 2By similarity

GO - Molecular functioni

  • [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: RGD
  • magnesium-dependent protein serine/threonine phosphatase activity Source: RGD
  • magnesium ion binding Source: RGD
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  • carbohydrate utilization Source: RGD
  • peptidyl-threonine dephosphorylation Source: UniProtKB
  • protein dephosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.43. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial (EC:3.1.3.43)
Short name:
PDP 2
Alternative name(s):
Pyruvate dehydrogenase phosphatase catalytic subunit 2
Short name:
PDPC 2
Gene namesi
Name:Pdp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628812. Pdp2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial pyruvate dehydrogenase (lipoamide) phosphatase complex Source: RGD
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6767MitochondrionSequence analysisAdd
BLAST
Chaini68 – 530463[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrialPRO_0000025422Add
BLAST

Proteomic databases

PaxDbiO88484.
PRIDEiO88484.

Expressioni

Tissue specificityi

Highly expressed in liver.

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit and a FAD protein of unknown function.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016462.

Structurei

3D structure databases

ProteinModelPortaliO88484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 518412PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0700. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000220821.
HOVERGENiHBG008162.
InParanoidiO88484.
KOiK01102.
OrthoDBiEOG7KQ219.
PhylomeDBiO88484.
TreeFamiTF313505.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 5 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTASYRIF NFARNRIAVL RGGRRLYSRA ATSRNQVKWR LFSPASLAVN
60 70 80 90 100
DSSPHGGFAL RKAYRHTSTE EEDFHLQLSP EQVSDLLRAG ESSHKVLDFN
110 120 130 140 150
SGVPNSVLRF ESNQLAANSP VEDRQGVASC VQTRGTVFGI FDGHGGHACA
160 170 180 190 200
QAVSERLFYY MAVSLMSHKT LEQMEEAMEN MKPLLPILQW LKHPGDSIYK
210 220 230 240 250
DITSVHLDHL RVYWQELLDL HMETGLSTEE ALMYSFQRLD SDISLEIQAP
260 270 280 290 300
LEDEVTKNLS LQVAFSGATA CMAHVDGVHL HIANAGDCRA ILGVQGDNGA
310 320 330 340 350
WSCLPLTCDH NAWNEAELSR LKREHPESED RTLIIDDRLL GVLLPCRAFG
360 370 380 390 400
DVQLKWSKEL QRNVLERGFD TEALNIYQFT PPHYHTPPYL TAKPEVTYHR
410 420 430 440 450
LRPQDKFLVL ASDGLWDMLD NEDVVRLVVG HLSKVGHQKP ALDQRPANLG
460 470 480 490 500
HMQSLLLQRK ASGLHAADQN AATHLIRHAI GSNEYGEMEP ERLAAMLTLP
510 520 530
EDVARMYRDD ITVMVVFFNS ESIDTYFKEG
Length:530
Mass (Da):59,654
Last modified:November 1, 1998 - v1
Checksum:i5AB688FAC78AD9CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371V → M in AAH72485 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062741 mRNA. Translation: AAC40168.1.
BC072485 mRNA. Translation: AAH72485.1.
RefSeqiNP_659559.2. NM_145091.4.
XP_006255110.1. XM_006255048.2.
UniGeneiRn.30021.

Genome annotation databases

GeneIDi246311.
KEGGirno:246311.
UCSCiRGD:628812. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062741 mRNA. Translation: AAC40168.1.
BC072485 mRNA. Translation: AAH72485.1.
RefSeqiNP_659559.2. NM_145091.4.
XP_006255110.1. XM_006255048.2.
UniGeneiRn.30021.

3D structure databases

ProteinModelPortaliO88484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016462.

Proteomic databases

PaxDbiO88484.
PRIDEiO88484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246311.
KEGGirno:246311.
UCSCiRGD:628812. rat.

Organism-specific databases

CTDi57546.
RGDi628812. Pdp2.

Phylogenomic databases

eggNOGiKOG0700. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000220821.
HOVERGENiHBG008162.
InParanoidiO88484.
KOiK01102.
OrthoDBiEOG7KQ219.
PhylomeDBiO88484.
TreeFamiTF313505.

Enzyme and pathway databases

BRENDAi3.1.3.43. 5301.

Miscellaneous databases

PROiO88484.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 5 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino acid sequences, expression, and regulation."
    Huang B., Gudi R., Wu P., Harris R.A., Hamilton J., Popov K.M.
    J. Biol. Chem. 273:17680-17688(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiPDP2_RAT
AccessioniPrimary (citable) accession number: O88484
Secondary accession number(s): Q6IN27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.