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Protein

Forkhead box protein L2

Gene

Foxl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Is a regulator of CYP19 expression. Is a transcriptional repressor of STAR. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi50 – 144Fork-headPROSITE-ProRule annotationAdd BLAST95

GO - Molecular functioni

GO - Biological processi

  • apoptotic DNA fragmentation Source: UniProtKB
  • embryonic eye morphogenesis Source: MGI
  • extraocular skeletal muscle development Source: UniProtKB
  • female gonad development Source: MGI
  • female somatic sex determination Source: MGI
  • granulosa cell differentiation Source: MGI
  • menstruation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • oocyte growth Source: Ensembl
  • ovarian follicle development Source: MGI
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of follicle-stimulating hormone secretion Source: MGI
  • positive regulation of luteinizing hormone secretion Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • single fertilization Source: MGI
  • uterus development Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processDifferentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3232118. SUMOylation of transcription factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein L2
Alternative name(s):
Pituitary forkhead factor
Short name:
P-Frk
Gene namesi
Name:Foxl2
Synonyms:Pfrk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1349428. Foxl2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • intercellular bridge Source: MGI
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918621 – 375Forkhead box protein L2Add BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei33PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated with SUMO1; sumoylation is required for transcriptional repression activity.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88470.
PRIDEiO88470.

PTM databases

PhosphoSitePlusiO88470.

Expressioni

Tissue specificityi

Expressed in the mesenchyme of developing eyelids. Expressed in ovaries throughout development and adulthood, localized to the undifferentiated granulosa cells in small and medium follicles as well as cumulus cells of preovulatory follicles. Expressed in the pituitary.2 Publications

Gene expression databases

BgeeiENSMUSG00000050397.
CleanExiMM_FOXL2.
ExpressionAtlasiO88470. baseline and differential.
GenevisibleiO88470. MM.

Interactioni

Subunit structurei

Interacts with ESR1. Interacts with UBE2I/UBC9 (By similarity). Interacts with SMAD3. Interacts with DDX20.By similarity4 Publications

GO - Molecular functioni

  • estrogen receptor binding Source: UniProtKB
  • ubiquitin conjugating enzyme binding Source: MGI

Protein-protein interaction databases

BioGridi205068. 25 interactors.
IntActiO88470. 2 interactors.
STRINGi10090.ENSMUSP00000053297.

Structurei

3D structure databases

ProteinModelPortaliO88470.
SMRiO88470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi217 – 230Poly-AlaAdd BLAST14
Compositional biasi280 – 287Poly-Pro8
Compositional biasi300 – 303Poly-Ala4

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00760000118904.
HOGENOMiHOG000218632.
HOVERGENiHBG051651.
InParanoidiO88470.
KOiK09405.
OMAiSMMHCSY.
OrthoDBiEOG091G0ESH.
PhylomeDBiO88470.
TreeFamiTF316127.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001766. Fork_head_dom.
IPR033063. FoxL2.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR11829:SF240. PTHR11829:SF240. 1 hit.
PfamiView protein in Pfam
PF00250. Forkhead. 1 hit.
PRINTSiPR00053. FORKHEAD.
SMARTiView protein in SMART
SM00339. FH. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.

Sequencei

Sequence statusi: Complete.

O88470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASYPEPED TAGTLLAPES GRAVKEAEAS PPSPGKGGGT TPEKPDPAQK
60 70 80 90 100
PPYSYVALIA MAIRESAEKR LTLSGIYQYI IAKFPFYEKN KKGWQNSIRH
110 120 130 140 150
NLSLNECFIK VPREGGGERK GNYWTLDPAC EDMFEKGNYR RRRRMKRPFR
160 170 180 190 200
PPPAHFQPGK GLFGSGGAAG GCGVPGAGAD GYGYLAPPKY LQSGFLNNSW
210 220 230 240 250
PLPQPPSPMP YASCQMAAAA AAAAAAAAAA GPGSPGAAAV VKGLAGPAAS
260 270 280 290 300
YGPYSRVQSM ALPPGVVNSY NGLGGPPAAP PPPPPPPHPH PHPHAHHLHA
310 320 330 340 350
AAAPPPAPPH HGAAAPPPGQ LSPASPATAA PPAPAPTSAP GLQFACARQP
360 370
ELAMMHCSYW DHDSKTGALH SRLDL
Length:375
Mass (Da):38,887
Last modified:March 15, 2005 - v2
Checksum:iF920C8407686C4B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF522275 Genomic DNA. Translation: AAN04088.1.
AF060873 mRNA. Translation: AAC27508.1.
CCDSiCCDS23430.1.
RefSeqiNP_036150.1. NM_012020.2.
UniGeneiMm.151239.

Genome annotation databases

EnsembliENSMUST00000051312; ENSMUSP00000053297; ENSMUSG00000050397.
GeneIDi26927.
KEGGimmu:26927.
UCSCiuc009rdr.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFOXL2_MOUSE
AccessioniPrimary (citable) accession number: O88470
Secondary accession number(s): Q8K3X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 15, 2005
Last modified: June 7, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot