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Protein

Calpain small subunit 1

Gene

Capns1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi113Calcium 1By similarity1
Metal bindingi115Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi120Calcium 1By similarity1
Metal bindingi138Calcium 4By similarity1
Metal bindingi153Calcium 2By similarity1
Metal bindingi155Calcium 2By similarity1
Metal bindingi157Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi159Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi164Calcium 2By similarity1
Metal bindingi183Calcium 3By similarity1
Metal bindingi185Calcium 3By similarity1
Metal bindingi187Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi189Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi194Calcium 3By similarity1
Metal bindingi226Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi109 – 1201PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi153 – 1642PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi183 – 1943PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: MGI
  • calcium ion binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B24. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain small subunit 1
Short name:
CSS1
Alternative name(s):
Calcium-activated neutral proteinase small subunit
Short name:
CANP small subunit
Calcium-dependent protease small subunit
Short name:
CDPS
Calcium-dependent protease small subunit 1
Calpain regulatory subunit
Gene namesi
Name:Capns1
Synonyms:Capn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88266. Capns1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon calcium binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737141 – 269Calpain small subunit 1Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei180N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88456.
MaxQBiO88456.
PaxDbiO88456.
PeptideAtlasiO88456.
PRIDEiO88456.

PTM databases

iPTMnetiO88456.
PhosphoSitePlusiO88456.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001794.
CleanExiMM_CAPNS1.

Interactioni

Subunit structurei

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).By similarity

Protein-protein interaction databases

BioGridi198473. 2 interactors.
IntActiO88456. 1 interactor.
MINTiMINT-4091916.
STRINGi10090.ENSMUSP00000001845.

Structurei

3D structure databases

ProteinModelPortaliO88456.
SMRiO88456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 131EF-hand 1; atypicalPROSITE-ProRule annotationAdd BLAST35
Domaini140 – 173EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini170 – 205EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini206 – 234EF-hand 4PROSITE-ProRule annotationAdd BLAST29
Domaini235 – 269EF-hand 5PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 65Gly-rich (hydrophobic)Add BLAST65
Compositional biasi10 – 25Poly-GlyAdd BLAST16
Compositional biasi37 – 57Poly-GlyAdd BLAST21
Compositional biasi79 – 84Poly-Pro6

Domaini

The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.By similarity
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiO88456.
KOiK08583.
PhylomeDBiO88456.
TreeFamiTF314682.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVNSFLKG GGGGGGGGGL GGGLGNVLGG LISGAAGGGG GGGGGGMGLG
60 70 80 90 100
GGGGGGGTAM RILGGVISAI SEAAAQYNPE PPPPRSHYSN IEANESEEVR
110 120 130 140 150
QFRKLFVQLA GDDMEVSATE LMNILNKVVT RHPDLKTDGF GIDTCRSMVA
160 170 180 190 200
VMDSDTTGKL GFEEFKYLWN NIKKWQAIYK RFDTDRSGTI GSHELPGAFE
210 220 230 240 250
AAGFHLNEHL YSMIIRRYAD ESGNMDFDNF ISCLVRLDAM FRAFKSLDKN
260
GTGQIQVNIQ EWLQLTMYS
Length:269
Mass (Da):28,463
Last modified:November 1, 1998 - v1
Checksum:iC578771942FB57E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46Missing in AAH18352 (PubMed:15489334).Curated1
Sequence conflicti46Missing in AAH90988 (PubMed:15489334).Curated1
Sequence conflicti69 – 70AI → PL in BAC25743 (PubMed:16141072).Curated2
Sequence conflicti70I → M in BAE21378 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139373 Genomic DNA. Translation: AAD38363.2.
AF058298 mRNA. Translation: AAC97194.1.
BC090988 mRNA. Translation: AAH90988.1.
BC018352 mRNA. Translation: AAH18352.1.
AK028092 mRNA. Translation: BAC25743.1.
AK132819 mRNA. Translation: BAE21378.1.
RefSeqiNP_033925.2. NM_009795.3.
XP_006539551.1. XM_006539488.2.
UniGeneiMm.439782.

Genome annotation databases

GeneIDi12336.
KEGGimmu:12336.
UCSCiuc009gdq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139373 Genomic DNA. Translation: AAD38363.2.
AF058298 mRNA. Translation: AAC97194.1.
BC090988 mRNA. Translation: AAH90988.1.
BC018352 mRNA. Translation: AAH18352.1.
AK028092 mRNA. Translation: BAC25743.1.
AK132819 mRNA. Translation: BAE21378.1.
RefSeqiNP_033925.2. NM_009795.3.
XP_006539551.1. XM_006539488.2.
UniGeneiMm.439782.

3D structure databases

ProteinModelPortaliO88456.
SMRiO88456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198473. 2 interactors.
IntActiO88456. 1 interactor.
MINTiMINT-4091916.
STRINGi10090.ENSMUSP00000001845.

PTM databases

iPTMnetiO88456.
PhosphoSitePlusiO88456.

Proteomic databases

EPDiO88456.
MaxQBiO88456.
PaxDbiO88456.
PeptideAtlasiO88456.
PRIDEiO88456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12336.
KEGGimmu:12336.
UCSCiuc009gdq.1. mouse.

Organism-specific databases

CTDi826.
MGIiMGI:88266. Capns1.

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiO88456.
KOiK08583.
PhylomeDBiO88456.
TreeFamiTF314682.

Enzyme and pathway databases

BRENDAi3.4.22.B24. 3474.

Miscellaneous databases

ChiTaRSiCapns1. mouse.
PROiO88456.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001794.
CleanExiMM_CAPNS1.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPNS1_MOUSE
AccessioniPrimary (citable) accession number: O88456
Secondary accession number(s): Q3V0X5
, Q5BKQ2, Q8CEI2, Q8VEK4, Q9R1C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.