Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

7-dehydrocholesterol reductase

Gene

Dhcr7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

Catalytic activityi

Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.

Pathwayi: cholesterol biosynthesis

This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei354NADPBy similarity1
Binding sitei358NADPBy similarity1
Binding sitei391NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei396NADPBy similarity1
Binding sitei443NADPBy similarity1
Binding sitei458NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi403 – 404NADPBy similarity2
Nucleotide bindingi447 – 451NADPBy similarity5

GO - Molecular functioni

  • 7-dehydrocholesterol reductase activity Source: MGI
  • NADP binding Source: UniProtKB

GO - Biological processi

  • blood vessel development Source: MGI
  • cell differentiation Source: MGI
  • cholesterol biosynthetic process Source: MGI
  • lung development Source: MGI
  • multicellular organism growth Source: MGI
  • post-embryonic development Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of cholesterol biosynthetic process Source: Ensembl
  • sterol biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-2426168. Activation of gene expression by SREBF (SREBP).
R-MMU-6807047. Cholesterol biosynthesis via desmosterol.
R-MMU-6807062. Cholesterol biosynthesis via lathosterol.
UniPathwayiUPA00063.

Chemistry databases

SwissLipidsiSLP:000001320.

Names & Taxonomyi

Protein namesi
Recommended name:
7-dehydrocholesterol reductase (EC:1.3.1.21)
Short name:
7-DHC reductase
Alternative name(s):
Sterol Delta(7)-reductase
Gene namesi
Name:Dhcr7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1298378. Dhcr7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002075031 – 4717-dehydrocholesterol reductaseAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88455.
MaxQBiO88455.
PaxDbiO88455.
PeptideAtlasiO88455.
PRIDEiO88455.
TopDownProteomicsiO88455.

PTM databases

iPTMnetiO88455.
PhosphoSitePlusiO88455.

Expressioni

Gene expression databases

BgeeiENSMUSG00000058454.
CleanExiMM_DHCR7.
ExpressionAtlasiO88455. baseline and differential.
GenevisibleiO88455. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073541.

Structurei

3D structure databases

ProteinModelPortaliO88455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG4/ERG24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1435. Eukaryota.
ENOG410XP67. LUCA.
GeneTreeiENSGT00390000000417.
HOGENOMiHOG000193296.
HOVERGENiHBG007825.
InParanoidiO88455.
KOiK00213.
OrthoDBiEOG091G0F22.
PhylomeDBiO88455.
TreeFamiTF101180.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKSQHNAP KVKSPNGKAG SQGQWGRAWE VDWFSLASII FLLLFAPFIV
60 70 80 90 100
YYFIMACDQY SCSLTAPALD IATGHASLAD IWAKTPPVTA KAAQLYALWV
110 120 130 140 150
SFQVLLYSWL PDFCHRFLPG YVGGVQEGAI TPAGVVNKYE VNGLQAWLIT
160 170 180 190 200
HILWFVNAYL LSWFSPTIIF DNWIPLLWCA NILGYAVSTF AMIKGYLFPT
210 220 230 240 250
SAEDCKFTGN FFYNYMMGIE FNPRIGKWFD FKLFFNGRPG IVAWTLINLS
260 270 280 290 300
FAAKQQELYG HVTNSMILVN VLQAIYVLDF FWNETWYLKT IDICHDHFGW
310 320 330 340 350
YLGWGDCVWL PYLYTLQGLY LVYHPVQLST PNALGILLLG LVGYYIFRMT
360 370 380 390 400
NHQKDLFRRT DGRCLIWGKK PKAIECSYTS ADGLKHHSKL LVSGFWGVAR
410 420 430 440 450
HFNYTGDLMG SLAYCLACGG GHLLPYFYII YMTILLTHRC LRDEHRCANK
460 470
YGRDWERYTA AVPYRLLPGI F
Length:471
Mass (Da):53,919
Last modified:November 1, 1998 - v1
Checksum:i6B1BC356CC539290
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057368 mRNA. Translation: AAC40164.1.
BC006854 mRNA. Translation: AAH06854.1.
CCDSiCCDS22048.1.
RefSeqiNP_031882.1. NM_007856.2.
XP_006508541.1. XM_006508478.1.
XP_006508542.1. XM_006508479.3.
XP_006508543.1. XM_006508480.2.
XP_006508544.1. XM_006508481.2.
UniGeneiMm.249342.

Genome annotation databases

EnsembliENSMUST00000073878; ENSMUSP00000073541; ENSMUSG00000058454.
ENSMUST00000124340; ENSMUSP00000117659; ENSMUSG00000058454.
ENSMUST00000141916; ENSMUSP00000121782; ENSMUSG00000058454.
GeneIDi13360.
KEGGimmu:13360.
UCSCiuc009kqc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057368 mRNA. Translation: AAC40164.1.
BC006854 mRNA. Translation: AAH06854.1.
CCDSiCCDS22048.1.
RefSeqiNP_031882.1. NM_007856.2.
XP_006508541.1. XM_006508478.1.
XP_006508542.1. XM_006508479.3.
XP_006508543.1. XM_006508480.2.
XP_006508544.1. XM_006508481.2.
UniGeneiMm.249342.

3D structure databases

ProteinModelPortaliO88455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073541.

Chemistry databases

SwissLipidsiSLP:000001320.

PTM databases

iPTMnetiO88455.
PhosphoSitePlusiO88455.

Proteomic databases

EPDiO88455.
MaxQBiO88455.
PaxDbiO88455.
PeptideAtlasiO88455.
PRIDEiO88455.
TopDownProteomicsiO88455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073878; ENSMUSP00000073541; ENSMUSG00000058454.
ENSMUST00000124340; ENSMUSP00000117659; ENSMUSG00000058454.
ENSMUST00000141916; ENSMUSP00000121782; ENSMUSG00000058454.
GeneIDi13360.
KEGGimmu:13360.
UCSCiuc009kqc.1. mouse.

Organism-specific databases

CTDi1717.
MGIiMGI:1298378. Dhcr7.

Phylogenomic databases

eggNOGiKOG1435. Eukaryota.
ENOG410XP67. LUCA.
GeneTreeiENSGT00390000000417.
HOGENOMiHOG000193296.
HOVERGENiHBG007825.
InParanoidiO88455.
KOiK00213.
OrthoDBiEOG091G0F22.
PhylomeDBiO88455.
TreeFamiTF101180.

Enzyme and pathway databases

UniPathwayiUPA00063.
ReactomeiR-MMU-2426168. Activation of gene expression by SREBF (SREBP).
R-MMU-6807047. Cholesterol biosynthesis via desmosterol.
R-MMU-6807062. Cholesterol biosynthesis via lathosterol.

Miscellaneous databases

PROiO88455.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058454.
CleanExiMM_DHCR7.
ExpressionAtlasiO88455. baseline and differential.
GenevisibleiO88455. MM.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHCR7_MOUSE
AccessioniPrimary (citable) accession number: O88455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.