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Protein

Potassium channel subfamily K member 4

Gene

Kcnk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).By similarity2 Publications

Enzyme regulationi

Activated by arachidonic acid and other unsaturated fatty acids (PubMed:9628867). Not affected by volatile general anesthetics such as chloroform, diethyl ether, halothane and isoflurane (PubMed:10321245).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to alkaline pH Source: UniProtKB
  • cellular response to fatty acid Source: UniProtKB
  • cellular response to mechanical stimulus Source: MGI
  • cellular response to temperature stimulus Source: UniProtKB
  • detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • potassium ion transmembrane transport Source: UniProtKB
  • sensory perception of pain Source: UniProtKB
  • sensory perception of temperature stimulus Source: UniProtKB
  • stabilization of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_308973. TWIK related potassium channel (TREK).

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name:
TRAAK
Gene namesi
Name:Kcnk4
Synonyms:Traak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1298234. Kcnk4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicBy similarity
Transmembranei4 – 2421HelicalBy similarityAdd
BLAST
Topological domaini25 – 8864ExtracellularBy similarityAdd
BLAST
Intramembranei89 – 10315Helical; Name=Pore helix 1By similarityAdd
BLAST
Intramembranei104 – 1107By similarity
Topological domaini111 – 1188ExtracellularBy similarity
Transmembranei119 – 15133HelicalBy similarityAdd
BLAST
Topological domaini152 – 17322CytoplasmicBy similarityAdd
BLAST
Transmembranei174 – 19522HelicalBy similarityAdd
BLAST
Topological domaini196 – 2005ExtracellularBy similarity
Intramembranei201 – 21414Helical; Name=Pore helix 2By similarityAdd
BLAST
Intramembranei215 – 2206By similarity
Topological domaini221 – 23414ExtracellularBy similarityAdd
BLAST
Transmembranei235 – 26127HelicalBy similarityAdd
BLAST
Topological domaini262 – 398137CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice show increased sensitivity to pain caused by pressure, but also by heat.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Potassium channel subfamily K member 4PRO_0000101748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 – 52InterchainBy similarity
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO88454.

PTM databases

PhosphoSiteiO88454.

Expressioni

Tissue specificityi

Expressed in brain, spinal cord and eye. Not detected in heart, skeletal muscle, liver, lungs, kidney and testis.1 Publication

Gene expression databases

BgeeiO88454.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline.
GenevisibleiO88454. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Structurei

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1096Selectivity filter 1By similarity
Regioni213 – 2186Selectivity filter 2By similarity

Domaini

Channel opening is brought about by a conformation change that involves buckling of the second transmembrane helix and affects the position and orientation of the fourth transmembrane helix.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG280717.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiAYQPLVW.
OrthoDBiEOG77M8NK.
PhylomeDBiO88454.
TreeFamiTF313947.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88454-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQAQKKMD HGRDQFLRDH
60 70 80 90 100
PCVSQKSLED FIKLLVEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT
110 120 130 140 150
IITTIGYGNI VLHTDAGRLF CIFYALVGIP LFGMLLAGVG DRLGSSLRRG
160 170 180 190 200
IGHIEAIFLK WHVPPGLVRS LSAVLFLLIG CLLFVLTPTF VFSYMESWSK
210 220 230 240 250
LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI LFGLAYFASV
260 270 280 290 300
LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK
310 320 330 340 350
EQPLLPSSLP APPAVVEPAG RPGSPAPAEK VETPSPPTAS ALDYPSENLA
360 370 380 390
FIDESSDTQS ERGCALPRAP RGRRRPNPSK KPSRPRGPGR LRDKAVPV
Length:398
Mass (Da):43,052
Last modified:November 1, 1998 - v1
Checksum:i478A834B7B7AEC92
GO
Isoform 2 (identifier: O88454-2) [UniParc]FASTAAdd to basket

Also known as: TRAAKT, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     63-67: KLLVE → KAMAI
     68-398: Missing.

Show »
Length:67
Mass (Da):7,594
Checksum:i797A27D595231704
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei63 – 675KLLVE → KAMAI in isoform 2. CuratedVSP_006690
Alternative sequencei68 – 398331Missing in isoform 2. CuratedVSP_006691Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.1. [O88454-1]
UniGeneiMm.12894.

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.1. [O88454-1]
UniGeneiMm.12894.

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiO88454.

Proteomic databases

PRIDEiO88454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Organism-specific databases

CTDi50801.
MGIiMGI:1298234. Kcnk4.

Phylogenomic databases

eggNOGiNOG280717.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiAYQPLVW.
OrthoDBiEOG77M8NK.
PhylomeDBiO88454.
TreeFamiTF313947.

Enzyme and pathway databases

ReactomeiREACT_308973. TWIK related potassium channel (TREK).

Miscellaneous databases

NextBioi289931.
PROiO88454.
SOURCEiSearch...

Gene expression databases

BgeeiO88454.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline.
GenevisibleiO88454. MM.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.
ProtoNetiSearch...

Publicationsi

  1. "A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids."
    Fink M., Lesage F., Duprat F., Heurteaux C., Reyes R., Fosset M., Lazdunski M.
    EMBO J. 17:3297-3308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Inhalational anesthetics activate two-pore-domain background K+ channels."
    Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M.
    Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  3. "The mechano-activated K+ channels TRAAK and TREK-1 control both warm and cold perception."
    Noel J., Zimmermann K., Busserolles J., Deval E., Alloui A., Diochot S., Guy N., Borsotto M., Reeh P., Eschalier A., Lazdunski M.
    EMBO J. 28:1308-1318(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiKCNK4_MOUSE
AccessioniPrimary (citable) accession number: O88454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.