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O88454 (KCNK4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name=TRAAK
Gene names
Name:Kcnk4
Synonyms:Traak
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K+ concentrations.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed in brain, spinal cord and eye. Not detected in heart, skeletal muscle, liver, lungs, kidney and testis.

Miscellaneous

Activated by arachidonic acid and other unsaturated fatty acids. Not affected by volatile general anaesthetics such as chloroform, diethyl ether, halothane and isoflurane.

Sequence similarities

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Potassium channel
Voltage-gated channel
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpotassium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

voltage-gated ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O88454-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O88454-2)

Also known as: TRAAKT; Truncated;

The sequence of this isoform differs from the canonical sequence as follows:
     63-67: KLLVE → KAMAI
     68-398: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Potassium channel subfamily K member 4
PRO_0000101748

Regions

Topological domain1 – 33Cytoplasmic Potential
Transmembrane4 – 2421Helical; Potential
Intramembrane89 – 11325Pore-forming; Name=Pore-forming 1; Potential
Transmembrane119 – 13921Helical; Potential
Topological domain140 – 17132Cytoplasmic Potential
Transmembrane172 – 19221Helical; Potential
Intramembrane198 – 22225Pore-forming; Name=Pore-forming 2; Potential
Transmembrane235 – 25521Helical; Potential
Topological domain256 – 398143Cytoplasmic Potential

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Disulfide bond52Interchain By similarity

Natural variations

Alternative sequence63 – 675KLLVE → KAMAI in isoform 2.
VSP_006690
Alternative sequence68 – 398331Missing in isoform 2.
VSP_006691

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 478A834B7B7AEC92

FASTA39843,052
        10         20         30         40         50         60 
MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQAQKKMD HGRDQFLRDH PCVSQKSLED 

        70         80         90        100        110        120 
FIKLLVEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT IITTIGYGNI VLHTDAGRLF 

       130        140        150        160        170        180 
CIFYALVGIP LFGMLLAGVG DRLGSSLRRG IGHIEAIFLK WHVPPGLVRS LSAVLFLLIG 

       190        200        210        220        230        240 
CLLFVLTPTF VFSYMESWSK LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI 

       250        260        270        280        290        300 
LFGLAYFASV LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK 

       310        320        330        340        350        360 
EQPLLPSSLP APPAVVEPAG RPGSPAPAEK VETPSPPTAS ALDYPSENLA FIDESSDTQS 

       370        380        390 
ERGCALPRAP RGRRRPNPSK KPSRPRGPGR LRDKAVPV 

« Hide

Isoform 2 (TRAAKT) (Truncated) [UniParc].

Checksum: 797A27D595231704
Show »

FASTA677,594

References

[1]"A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids."
Fink M., Lesage F., Duprat F., Heurteaux C., Reyes R., Fosset M., Lazdunski M.
EMBO J. 17:3297-3308(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Inhalational anesthetics activate two-pore-domain background K+ channels."
Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M.
Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF056492 mRNA. Translation: AAC40181.1.
RefSeqNP_032457.1. NM_008431.2.
XP_006526783.1. XM_006526720.1.
UniGeneMm.12894.

3D structure databases

ProteinModelPortalO88454.
SMRO88454. Positions 2-259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000025908.

Protein family/group databases

TCDB1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteO88454.

Proteomic databases

PRIDEO88454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneID16528.
KEGGmmu:16528.
UCSCuc008gjj.1. mouse. [O88454-1]

Organism-specific databases

CTD50801.
MGIMGI:1298234. Kcnk4.

Phylogenomic databases

eggNOGNOG280717.
HOGENOMHOG000013106.
HOVERGENHBG052234.
InParanoidO88454.
KOK04915.
OMAAYQPLVW.
OrthoDBEOG77M8NK.
PhylomeDBO88454.
TreeFamTF313947.

Gene expression databases

ArrayExpressO88454.
BgeeO88454.
CleanExMM_KCNK4.
GenevestigatorO88454.

Family and domain databases

InterProIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.
ProtoNetSearch...

Other

NextBio289931.
PROO88454.
SOURCESearch...

Entry information

Entry nameKCNK4_MOUSE
AccessionPrimary (citable) accession number: O88454
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot