Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium channel subfamily K member 4

Gene

Kcnk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K+ concentrations.

GO - Molecular functioni

  1. potassium channel activity Source: UniProtKB-KW
  2. voltage-gated ion channel activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199083. TWIK related potassium channel (TREK).

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name:
TRAAK
Gene namesi
Name:Kcnk4
Synonyms:Traak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1298234. Kcnk4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence Analysis
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST
Intramembranei89 – 11325Pore-forming; Name=Pore-forming 1Sequence AnalysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence AnalysisAdd
BLAST
Topological domaini140 – 17132CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Intramembranei198 – 22225Pore-forming; Name=Pore-forming 2Sequence AnalysisAdd
BLAST
Transmembranei235 – 25521HelicalSequence AnalysisAdd
BLAST
Topological domaini256 – 398143CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Potassium channel subfamily K member 4PRO_0000101748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 – 52InterchainBy similarity
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO88454.

PTM databases

PhosphoSiteiO88454.

Expressioni

Tissue specificityi

Expressed in brain, spinal cord and eye. Not detected in heart, skeletal muscle, liver, lungs, kidney and testis.

Gene expression databases

BgeeiO88454.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline.
GenevestigatoriO88454.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Structurei

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454. Positions 2-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG280717.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiAYQPLVW.
OrthoDBiEOG77M8NK.
PhylomeDBiO88454.
TreeFamiTF313947.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O88454-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQAQKKMD HGRDQFLRDH
60 70 80 90 100
PCVSQKSLED FIKLLVEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT
110 120 130 140 150
IITTIGYGNI VLHTDAGRLF CIFYALVGIP LFGMLLAGVG DRLGSSLRRG
160 170 180 190 200
IGHIEAIFLK WHVPPGLVRS LSAVLFLLIG CLLFVLTPTF VFSYMESWSK
210 220 230 240 250
LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI LFGLAYFASV
260 270 280 290 300
LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK
310 320 330 340 350
EQPLLPSSLP APPAVVEPAG RPGSPAPAEK VETPSPPTAS ALDYPSENLA
360 370 380 390
FIDESSDTQS ERGCALPRAP RGRRRPNPSK KPSRPRGPGR LRDKAVPV
Length:398
Mass (Da):43,052
Last modified:November 1, 1998 - v1
Checksum:i478A834B7B7AEC92
GO
Isoform 2 (identifier: O88454-2) [UniParc]FASTAAdd to Basket

Also known as: TRAAKT, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     63-67: KLLVE → KAMAI
     68-398: Missing.

Show »
Length:67
Mass (Da):7,594
Checksum:i797A27D595231704
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei63 – 675KLLVE → KAMAI in isoform 2. CuratedVSP_006690
Alternative sequencei68 – 398331Missing in isoform 2. CuratedVSP_006691Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.1. [O88454-1]
UniGeneiMm.12894.

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.1. [O88454-1]
UniGeneiMm.12894.

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454. Positions 2-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiO88454.

Proteomic databases

PRIDEiO88454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Organism-specific databases

CTDi50801.
MGIiMGI:1298234. Kcnk4.

Phylogenomic databases

eggNOGiNOG280717.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiAYQPLVW.
OrthoDBiEOG77M8NK.
PhylomeDBiO88454.
TreeFamiTF313947.

Enzyme and pathway databases

ReactomeiREACT_199083. TWIK related potassium channel (TREK).

Miscellaneous databases

NextBioi289931.
PROiO88454.
SOURCEiSearch...

Gene expression databases

BgeeiO88454.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline.
GenevestigatoriO88454.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.
ProtoNetiSearch...

Publicationsi

  1. "A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids."
    Fink M., Lesage F., Duprat F., Heurteaux C., Reyes R., Fosset M., Lazdunski M.
    EMBO J. 17:3297-3308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Inhalational anesthetics activate two-pore-domain background K+ channels."
    Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M.
    Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVATION.

Entry informationi

Entry nameiKCNK4_MOUSE
AccessioniPrimary (citable) accession number: O88454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activated by arachidonic acid and other unsaturated fatty acids. Not affected by volatile general anaesthetics such as chloroform, diethyl ether, halothane and isoflurane.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.