Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O88454

- KCNK4_MOUSE

UniProt

O88454 - KCNK4_MOUSE

Protein

Potassium channel subfamily K member 4

Gene

Kcnk4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K+ concentrations.

    GO - Molecular functioni

    1. potassium channel activity Source: UniProtKB-KW
    2. voltage-gated ion channel activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199083. TWIK related potassium channel (TREK).

    Protein family/group databases

    TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel subfamily K member 4
    Alternative name(s):
    TWIK-related arachidonic acid-stimulated potassium channel protein
    Short name:
    TRAAK
    Gene namesi
    Name:Kcnk4
    Synonyms:Traak
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1298234. Kcnk4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Potassium channel subfamily K member 4PRO_0000101748Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 – 52InterchainBy similarity
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO88454.

    PTM databases

    PhosphoSiteiO88454.

    Expressioni

    Tissue specificityi

    Expressed in brain, spinal cord and eye. Not detected in heart, skeletal muscle, liver, lungs, kidney and testis.

    Gene expression databases

    ArrayExpressiO88454.
    BgeeiO88454.
    CleanExiMM_KCNK4.
    GenevestigatoriO88454.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025908.

    Structurei

    3D structure databases

    ProteinModelPortaliO88454.
    SMRiO88454. Positions 2-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 33CytoplasmicSequence Analysis
    Topological domaini140 – 17132CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini256 – 398143CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei89 – 11325Pore-forming; Name=Pore-forming 1Sequence AnalysisAdd
    BLAST
    Intramembranei198 – 22225Pore-forming; Name=Pore-forming 2Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei119 – 13921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei235 – 25521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG280717.
    HOGENOMiHOG000013106.
    HOVERGENiHBG052234.
    InParanoidiO88454.
    KOiK04915.
    OMAiAYQPLVW.
    OrthoDBiEOG77M8NK.
    PhylomeDBiO88454.
    TreeFamiTF313947.

    Family and domain databases

    InterProiIPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR008074. 2pore_dom_K_chnl_TRAAK.
    [Graphical view]
    PfamiPF07885. Ion_trans_2. 2 hits.
    [Graphical view]
    PRINTSiPR01333. 2POREKCHANEL.
    PR01691. TRAAKCHANNEL.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O88454-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQAQKKMD HGRDQFLRDH    50
    PCVSQKSLED FIKLLVEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT 100
    IITTIGYGNI VLHTDAGRLF CIFYALVGIP LFGMLLAGVG DRLGSSLRRG 150
    IGHIEAIFLK WHVPPGLVRS LSAVLFLLIG CLLFVLTPTF VFSYMESWSK 200
    LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI LFGLAYFASV 250
    LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK 300
    EQPLLPSSLP APPAVVEPAG RPGSPAPAEK VETPSPPTAS ALDYPSENLA 350
    FIDESSDTQS ERGCALPRAP RGRRRPNPSK KPSRPRGPGR LRDKAVPV 398
    Length:398
    Mass (Da):43,052
    Last modified:November 1, 1998 - v1
    Checksum:i478A834B7B7AEC92
    GO
    Isoform 2 (identifier: O88454-2) [UniParc]FASTAAdd to Basket

    Also known as: TRAAKT, Truncated

    The sequence of this isoform differs from the canonical sequence as follows:
         63-67: KLLVE → KAMAI
         68-398: Missing.

    Show »
    Length:67
    Mass (Da):7,594
    Checksum:i797A27D595231704
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei63 – 675KLLVE → KAMAI in isoform 2. CuratedVSP_006690
    Alternative sequencei68 – 398331Missing in isoform 2. CuratedVSP_006691Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056492 mRNA. Translation: AAC40181.1.
    CCDSiCCDS29511.1. [O88454-1]
    RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
    XP_006526783.1. XM_006526720.1. [O88454-1]
    UniGeneiMm.12894.

    Genome annotation databases

    EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
    GeneIDi16528.
    KEGGimmu:16528.
    UCSCiuc008gjj.1. mouse. [O88454-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056492 mRNA. Translation: AAC40181.1 .
    CCDSi CCDS29511.1. [O88454-1 ]
    RefSeqi NP_032457.1. NM_008431.2. [O88454-1 ]
    XP_006526783.1. XM_006526720.1. [O88454-1 ]
    UniGenei Mm.12894.

    3D structure databases

    ProteinModelPortali O88454.
    SMRi O88454. Positions 2-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025908.

    Protein family/group databases

    TCDBi 1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

    PTM databases

    PhosphoSitei O88454.

    Proteomic databases

    PRIDEi O88454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025908 ; ENSMUSP00000025908 ; ENSMUSG00000024957 . [O88454-1 ]
    GeneIDi 16528.
    KEGGi mmu:16528.
    UCSCi uc008gjj.1. mouse. [O88454-1 ]

    Organism-specific databases

    CTDi 50801.
    MGIi MGI:1298234. Kcnk4.

    Phylogenomic databases

    eggNOGi NOG280717.
    HOGENOMi HOG000013106.
    HOVERGENi HBG052234.
    InParanoidi O88454.
    KOi K04915.
    OMAi AYQPLVW.
    OrthoDBi EOG77M8NK.
    PhylomeDBi O88454.
    TreeFami TF313947.

    Enzyme and pathway databases

    Reactomei REACT_199083. TWIK related potassium channel (TREK).

    Miscellaneous databases

    NextBioi 289931.
    PROi O88454.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O88454.
    Bgeei O88454.
    CleanExi MM_KCNK4.
    Genevestigatori O88454.

    Family and domain databases

    InterProi IPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR008074. 2pore_dom_K_chnl_TRAAK.
    [Graphical view ]
    Pfami PF07885. Ion_trans_2. 2 hits.
    [Graphical view ]
    PRINTSi PR01333. 2POREKCHANEL.
    PR01691. TRAAKCHANNEL.
    ProtoNeti Search...

    Publicationsi

    1. "A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids."
      Fink M., Lesage F., Duprat F., Heurteaux C., Reyes R., Fosset M., Lazdunski M.
      EMBO J. 17:3297-3308(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Inhalational anesthetics activate two-pore-domain background K+ channels."
      Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M.
      Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVATION.

    Entry informationi

    Entry nameiKCNK4_MOUSE
    AccessioniPrimary (citable) accession number: O88454
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Activated by arachidonic acid and other unsaturated fatty acids. Not affected by volatile general anaesthetics such as chloroform, diethyl ether, halothane and isoflurane.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3