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Protein

Potassium channel subfamily K member 4

Gene

Kcnk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).By similarity2 Publications

Enzyme regulationi

Activated by arachidonic acid and other unsaturated fatty acids (PubMed:9628867). Not affected by volatile general anesthetics such as chloroform, diethyl ether, halothane and isoflurane (PubMed:10321245).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to alkaline pH Source: UniProtKB
  • cellular response to fatty acid Source: UniProtKB
  • cellular response to mechanical stimulus Source: MGI
  • cellular response to temperature stimulus Source: UniProtKB
  • detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • memory Source: Ensembl
  • potassium ion transmembrane transport Source: UniProtKB
  • sensory perception of pain Source: UniProtKB
  • sensory perception of temperature stimulus Source: UniProtKB
  • stabilization of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1299503. TWIK related potassium channel (TREK).

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name:
TRAAK
Gene namesi
Name:Kcnk4
Synonyms:Traak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1298234. Kcnk4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicBy similarity3
Transmembranei4 – 24HelicalBy similarityAdd BLAST21
Topological domaini25 – 88ExtracellularBy similarityAdd BLAST64
Intramembranei89 – 103Helical; Name=Pore helix 1By similarityAdd BLAST15
Intramembranei104 – 110By similarity7
Topological domaini111 – 118ExtracellularBy similarity8
Transmembranei119 – 151HelicalBy similarityAdd BLAST33
Topological domaini152 – 173CytoplasmicBy similarityAdd BLAST22
Transmembranei174 – 195HelicalBy similarityAdd BLAST22
Topological domaini196 – 200ExtracellularBy similarity5
Intramembranei201 – 214Helical; Name=Pore helix 2By similarityAdd BLAST14
Intramembranei215 – 220By similarity6
Topological domaini221 – 234ExtracellularBy similarityAdd BLAST14
Transmembranei235 – 261HelicalBy similarityAdd BLAST27
Topological domaini262 – 398CytoplasmicBy similarityAdd BLAST137

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice show increased sensitivity to pain caused by pressure, but also by heat.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017481 – 398Potassium channel subfamily K member 4Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52InterchainBy similarity
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO88454.
PRIDEiO88454.

PTM databases

iPTMnetiO88454.
PhosphoSitePlusiO88454.

Expressioni

Tissue specificityi

Expressed in brain, spinal cord and eye. Not detected in heart, skeletal muscle, liver, lungs, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000024957.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline and differential.
GenevisibleiO88454. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Structurei

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 109Selectivity filter 1By similarity6
Regioni213 – 218Selectivity filter 2By similarity6

Domaini

Channel opening is brought about by a conformation change that involves buckling of the second transmembrane helix and affects the position and orientation of the fourth transmembrane helix.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiLDYPSEN.
OrthoDBiEOG091G0E3R.
PhylomeDBiO88454.
TreeFamiTF313947.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR008074. 2pore_dom_K_chnl_TRAAK.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88454-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQAQKKMD HGRDQFLRDH
60 70 80 90 100
PCVSQKSLED FIKLLVEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT
110 120 130 140 150
IITTIGYGNI VLHTDAGRLF CIFYALVGIP LFGMLLAGVG DRLGSSLRRG
160 170 180 190 200
IGHIEAIFLK WHVPPGLVRS LSAVLFLLIG CLLFVLTPTF VFSYMESWSK
210 220 230 240 250
LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI LFGLAYFASV
260 270 280 290 300
LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK
310 320 330 340 350
EQPLLPSSLP APPAVVEPAG RPGSPAPAEK VETPSPPTAS ALDYPSENLA
360 370 380 390
FIDESSDTQS ERGCALPRAP RGRRRPNPSK KPSRPRGPGR LRDKAVPV
Length:398
Mass (Da):43,052
Last modified:November 1, 1998 - v1
Checksum:i478A834B7B7AEC92
GO
Isoform 2 (identifier: O88454-2) [UniParc]FASTAAdd to basket
Also known as: TRAAKT, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     63-67: KLLVE → KAMAI
     68-398: Missing.

Show »
Length:67
Mass (Da):7,594
Checksum:i797A27D595231704
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00669063 – 67KLLVE → KAMAI in isoform 2. Curated5
Alternative sequenceiVSP_00669168 – 398Missing in isoform 2. CuratedAdd BLAST331

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.2. [O88454-1]
UniGeneiMm.12894.

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056492 mRNA. Translation: AAC40181.1.
CCDSiCCDS29511.1. [O88454-1]
RefSeqiNP_032457.1. NM_008431.2. [O88454-1]
XP_006526783.1. XM_006526720.2. [O88454-1]
UniGeneiMm.12894.

3D structure databases

ProteinModelPortaliO88454.
SMRiO88454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025908.

Protein family/group databases

TCDBi1.A.1.9.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiO88454.
PhosphoSitePlusiO88454.

Proteomic databases

PaxDbiO88454.
PRIDEiO88454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025908; ENSMUSP00000025908; ENSMUSG00000024957. [O88454-1]
GeneIDi16528.
KEGGimmu:16528.
UCSCiuc008gjj.1. mouse. [O88454-1]

Organism-specific databases

CTDi50801.
MGIiMGI:1298234. Kcnk4.

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiO88454.
KOiK04915.
OMAiLDYPSEN.
OrthoDBiEOG091G0E3R.
PhylomeDBiO88454.
TreeFamiTF313947.

Enzyme and pathway databases

ReactomeiR-MMU-1299503. TWIK related potassium channel (TREK).

Miscellaneous databases

PROiO88454.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024957.
CleanExiMM_KCNK4.
ExpressionAtlasiO88454. baseline and differential.
GenevisibleiO88454. MM.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR008074. 2pore_dom_K_chnl_TRAAK.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNK4_MOUSE
AccessioniPrimary (citable) accession number: O88454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.