Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O88453

- SAFB1_RAT

UniProt

O88453 - SAFB1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Scaffold attachment factor B1

Gene

Safb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).By similarity

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. protein kinase binding Source: RGD
  3. RNA binding Source: UniProtKB-KW
  4. sequence-specific DNA binding Source: RGD

GO - Biological processi

  1. regulation of mRNA processing Source: RGD
  2. regulation of transcription from RNA polymerase II promoter Source: RGD
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B1
Short name:
SAF-B
Short name:
SAF-B1
Gene namesi
Name:Safb
Synonyms:Safb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620613. Safb.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 931930Scaffold attachment factor B1PRO_0000081906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei55 – 551PhosphoserineBy similarity
Cross-linki230 – 230Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki316 – 316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei405 – 4051PhosphoserineBy similarity
Modified residuei406 – 4061PhosphoserineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei602 – 6021PhosphoserineBy similarity
Modified residuei604 – 6041PhosphoserineBy similarity
Modified residuei623 – 6231PhosphoserineBy similarity
Modified residuei626 – 6261PhosphoserineBy similarity
Modified residuei629 – 6291N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated by CDC-like kinase 2 (CLK2).
Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO88453.

PTM databases

PhosphoSiteiO88453.

Expressioni

Gene expression databases

GenevestigatoriO88453.

Interactioni

Subunit structurei

Monomer. Can form homodimers (By similarity). Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10. Interacts with SRPK1 and inhibits its activity (By similarity). Interacts with RBMX (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SRPK1Q96SB42EBI-539530,EBI-539478From a different organism.

Protein-protein interaction databases

BioGridi249003. 8 interactions.
IntActiO88453. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO88453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 6535SAPPROSITE-ProRule annotationAdd
BLAST
Domaini428 – 50679RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni550 – 816267Interacts with POLR2A; SFRS1; SFRS9 and TRA2BAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi621 – 63818Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi66 – 285220Glu-richAdd
BLAST
Compositional biasi634 – 855222Arg-richAdd
BLAST
Compositional biasi640 – 72889Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG078408.
InParanoidiO88453.
PhylomeDBiO88453.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88453-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAETLSGLGD SGAASAAAVS SAASETGTRR LSDLRVIDLR AELRKRNLTS
60 70 80 90 100
SGNKSVLMER LKKAIEEEGG NPDEIEVISE GNKKMPKRPS KGKKPEDEGV
110 120 130 140 150
EDNGLEENSG DGQEDVETSL ENLQDMDMMD ISVLDEADID NGSVADCVEE
160 170 180 190 200
EEEATLPEGL GLLRIGRLQS KGLPEQLQEL AIDDKEAINN VDTSSSDFTI
210 220 230 240 250
LQEMEEASLE PENEKILDIL GETCKSEPVK EEGSELEQPF AQATSSVGPD
260 270 280 290 300
RKLAEEEDLF ESCGHPEEEE EEEEEEEQEE EQEEEGDLAL ASSSKSESSS
310 320 330 340 350
TRCQWSEADA LLAVVKREPA EAPGGGTGMD REPVGLEEPV EQSSTAAQLP
360 370 380 390 400
ETTSQELVRA PTAAPSPEPR DSKDDVKKFA FDACNDVPAA PKESSASEGA
410 420 430 440 450
DQKMSSVEDD SDTKRLSREE KGRSSCGRNF WVSGLSSTTR ATDLKNLFSR
460 470 480 490 500
YGKVVGAKVV TNARSPGARC YGFVTMSTAE EATKCINHLH KTELHGKMIS
510 520 530 540 550
VEKAKSEPAG KRVPDRRDGD SKKEKTSTSD RSANLKREEK GDRKDDAKKT
560 570 580 590 600
DDGSTEKSKD ADDQKPGPSE RSRTTKSGSR GTERTVVMDK SKGVPVISVK
610 620 630 640 650
TSGSKERASK SQDRKSVSRE KRSVVSFDKV KESRKSRDSE SRRERERERS
660 670 680 690 700
EREQRLQAQW EREERERLEI ARERLAFHRH RLERERMERE RLERERMHVE
710 720 730 740 750
QERRREQERI HREREELRRQ QELRYEQERR PAVRRPYEVD GRRDDAYWPE
760 770 780 790 800
AKRAALDDRY HSDFSRQDRF HDFDHRDRGR YPNHSVDRRE GSRSMMGDRE
810 820 830 840 850
GQHYPERHGG PERHGRDSRD GWGYGSNKRL SEGRGLPLLP RRDWGEHARR
860 870 880 890 900
LEDDRAWQGT ADGGMMERDQ QRWQGGERSM SGHSGPGHMM NRGGMSGRGS
910 920 930
FAPGGASRRH VIPRGGMQAG FGGTEPGQQT Q
Length:931
Mass (Da):104,567
Last modified:November 14, 2003 - v2
Checksum:iA5E3FD7AB33341F6
GO

Sequence cautioni

The sequence AAC29479.1 differs from that shown. Reason: Frameshift at position 50. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056324 mRNA. Translation: AAC29479.1. Frameshift.
RefSeqiNP_071789.1. NM_022394.1.
UniGeneiRn.88640.

Genome annotation databases

GeneIDi64196.
KEGGirno:64196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056324 mRNA. Translation: AAC29479.1 . Frameshift.
RefSeqi NP_071789.1. NM_022394.1.
UniGenei Rn.88640.

3D structure databases

ProteinModelPortali O88453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249003. 8 interactions.
IntActi O88453. 1 interaction.

PTM databases

PhosphoSitei O88453.

Proteomic databases

PRIDEi O88453.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 64196.
KEGGi rno:64196.

Organism-specific databases

CTDi 6294.
RGDi 620613. Safb.

Phylogenomic databases

HOVERGENi HBG078408.
InParanoidi O88453.
PhylomeDBi O88453.

Miscellaneous databases

NextBioi 612872.
PROi O88453.

Gene expression databases

Genevestigatori O88453.

Family and domain databases

Gene3Di 1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view ]
Pfami PF02037. SAP. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH POLR2A; SFRS1; SFRS9 AND TRA2B.
  2. "The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites."
    Stoss O., Olbrich M., Hartmann A.M., Koenig H., Memmott J., Andreadis A., Stamm S.
    J. Biol. Chem. 276:8665-8673(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KHDRBS3.
    Tissue: Brain.

Entry informationi

Entry nameiSAFB1_RAT
AccessioniPrimary (citable) accession number: O88453
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3