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O88453

- SAFB1_RAT

UniProt

O88453 - SAFB1_RAT

Protein

Scaffold attachment factor B1

Gene

Safb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (14 Nov 2003)
      Previous versions | rss
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    Functioni

    Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. protein kinase binding Source: RGD
    4. RNA binding Source: UniProtKB-KW
    5. sequence-specific DNA binding Source: RGD

    GO - Biological processi

    1. regulation of mRNA processing Source: RGD
    2. regulation of transcription from RNA polymerase II promoter Source: RGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Scaffold attachment factor B1
    Short name:
    SAF-B
    Short name:
    SAF-B1
    Gene namesi
    Name:Safb
    Synonyms:Safb1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620613. Safb.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 931930Scaffold attachment factor B1PRO_0000081906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei55 – 551PhosphoserineBy similarity
    Cross-linki230 – 230Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki316 – 316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei405 – 4051PhosphoserineBy similarity
    Modified residuei406 – 4061PhosphoserineBy similarity
    Modified residuei437 – 4371PhosphoserineBy similarity
    Modified residuei602 – 6021PhosphoserineBy similarity
    Modified residuei604 – 6041PhosphoserineBy similarity
    Modified residuei623 – 6231PhosphoserineBy similarity
    Modified residuei626 – 6261PhosphoserineBy similarity
    Modified residuei629 – 6291N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by CDC-like kinase 2 (CLK2).
    Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiO88453.

    PTM databases

    PhosphoSiteiO88453.

    Expressioni

    Gene expression databases

    GenevestigatoriO88453.

    Interactioni

    Subunit structurei

    Monomer. Can form homodimers By similarity. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10. Interacts with SRPK1 and inhibits its activity By similarity. Interacts with RBMX By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SRPK1Q96SB42EBI-539530,EBI-539478From a different organism.

    Protein-protein interaction databases

    BioGridi249003. 8 interactions.
    IntActiO88453. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO88453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 6535SAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini428 – 50679RRMPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni550 – 816267Interacts with POLR2A; SFRS1; SFRS9 and TRA2BAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi621 – 63818Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi66 – 285220Glu-richAdd
    BLAST
    Compositional biasi634 – 855222Arg-richAdd
    BLAST
    Compositional biasi640 – 72889Glu-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
    Contains 1 SAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG078408.
    PhylomeDBiO88453.

    Family and domain databases

    Gene3Di1.10.720.30. 1 hit.
    3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR003034. SAP_dom.
    [Graphical view]
    PfamiPF02037. SAP. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00513. SAP. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS50800. SAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O88453-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAETLSGLGD SGAASAAAVS SAASETGTRR LSDLRVIDLR AELRKRNLTS    50
    SGNKSVLMER LKKAIEEEGG NPDEIEVISE GNKKMPKRPS KGKKPEDEGV 100
    EDNGLEENSG DGQEDVETSL ENLQDMDMMD ISVLDEADID NGSVADCVEE 150
    EEEATLPEGL GLLRIGRLQS KGLPEQLQEL AIDDKEAINN VDTSSSDFTI 200
    LQEMEEASLE PENEKILDIL GETCKSEPVK EEGSELEQPF AQATSSVGPD 250
    RKLAEEEDLF ESCGHPEEEE EEEEEEEQEE EQEEEGDLAL ASSSKSESSS 300
    TRCQWSEADA LLAVVKREPA EAPGGGTGMD REPVGLEEPV EQSSTAAQLP 350
    ETTSQELVRA PTAAPSPEPR DSKDDVKKFA FDACNDVPAA PKESSASEGA 400
    DQKMSSVEDD SDTKRLSREE KGRSSCGRNF WVSGLSSTTR ATDLKNLFSR 450
    YGKVVGAKVV TNARSPGARC YGFVTMSTAE EATKCINHLH KTELHGKMIS 500
    VEKAKSEPAG KRVPDRRDGD SKKEKTSTSD RSANLKREEK GDRKDDAKKT 550
    DDGSTEKSKD ADDQKPGPSE RSRTTKSGSR GTERTVVMDK SKGVPVISVK 600
    TSGSKERASK SQDRKSVSRE KRSVVSFDKV KESRKSRDSE SRRERERERS 650
    EREQRLQAQW EREERERLEI ARERLAFHRH RLERERMERE RLERERMHVE 700
    QERRREQERI HREREELRRQ QELRYEQERR PAVRRPYEVD GRRDDAYWPE 750
    AKRAALDDRY HSDFSRQDRF HDFDHRDRGR YPNHSVDRRE GSRSMMGDRE 800
    GQHYPERHGG PERHGRDSRD GWGYGSNKRL SEGRGLPLLP RRDWGEHARR 850
    LEDDRAWQGT ADGGMMERDQ QRWQGGERSM SGHSGPGHMM NRGGMSGRGS 900
    FAPGGASRRH VIPRGGMQAG FGGTEPGQQT Q 931
    Length:931
    Mass (Da):104,567
    Last modified:November 14, 2003 - v2
    Checksum:iA5E3FD7AB33341F6
    GO

    Sequence cautioni

    The sequence AAC29479.1 differs from that shown. Reason: Frameshift at position 50.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056324 mRNA. Translation: AAC29479.1. Frameshift.
    RefSeqiNP_071789.1. NM_022394.1.
    UniGeneiRn.88640.

    Genome annotation databases

    GeneIDi64196.
    KEGGirno:64196.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056324 mRNA. Translation: AAC29479.1 . Frameshift.
    RefSeqi NP_071789.1. NM_022394.1.
    UniGenei Rn.88640.

    3D structure databases

    ProteinModelPortali O88453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249003. 8 interactions.
    IntActi O88453. 1 interaction.

    PTM databases

    PhosphoSitei O88453.

    Proteomic databases

    PRIDEi O88453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 64196.
    KEGGi rno:64196.

    Organism-specific databases

    CTDi 6294.
    RGDi 620613. Safb.

    Phylogenomic databases

    HOVERGENi HBG078408.
    PhylomeDBi O88453.

    Miscellaneous databases

    NextBioi 612872.
    PROi O88453.

    Gene expression databases

    Genevestigatori O88453.

    Family and domain databases

    Gene3Di 1.10.720.30. 1 hit.
    3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR003034. SAP_dom.
    [Graphical view ]
    Pfami PF02037. SAP. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00513. SAP. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS50800. SAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, INTERACTION WITH POLR2A; SFRS1; SFRS9 AND TRA2B.
    2. "The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites."
      Stoss O., Olbrich M., Hartmann A.M., Koenig H., Memmott J., Andreadis A., Stamm S.
      J. Biol. Chem. 276:8665-8673(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KHDRBS3.
      Tissue: Brain.

    Entry informationi

    Entry nameiSAFB1_RAT
    AccessioniPrimary (citable) accession number: O88453
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: November 14, 2003
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3