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Protein

Deformed epidermal autoregulatory factor 1 homolog

Gene

Deaf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Transcriptional activator of EIF4G3. May also involved in behavior (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri504 – 540MYND-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deformed epidermal autoregulatory factor 1 homolog
Alternative name(s):
Nuclear DEAF-1-related transcriptional regulator
Short name:
NUDR
Suppressin
Gene namesi
Name:Deaf1
Synonyms:Spn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620671. Deaf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000740871 – 565Deformed epidermal autoregulatory factor 1 homologAdd BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphothreonineBy similarity1
Modified residuei176PhosphoserineCombined sources1
Modified residuei179PhosphothreonineBy similarity1
Modified residuei432PhosphothreonineBy similarity1
Modified residuei443PhosphoserineCombined sources1
Modified residuei448PhosphoserineCombined sources1

Post-translational modificationi

May be phosphorylated by DNA-PK complex in a DNA independent manner (in vitro).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88450.
PRIDEiO88450.

PTM databases

iPTMnetiO88450.
PhosphoSitePlusiO88450.

Expressioni

Tissue specificityi

Ubiquitous. Detected in brain, spleen, adrenal, lung, skeletal muscle, liver, kidney, and in developing germ cells in testis. In pituitary, restricted to hormone-secreting cell types.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with LMO4; LMO4 blocks export from nucleus. Interacts with LMO2 and CLIM2. May interact with the corepressors NCOR1 and NCRO2. Identified in a complex with XRCC5 and XRCC6. Interacts (via the SAND domain) with the DNA-PK complex subunit XRCC6; the interaction is direct and may be inhibited by DNA-binding (By similarity).By similarity

Protein-protein interaction databases

IntActiO88450. 1 interactor.
STRINGi10116.ENSRNOP00000046656.

Structurei

3D structure databases

ProteinModelPortaliO88450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 273SANDPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni403 – 478Interaction with LMO4By similarityAdd BLAST76

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi301 – 316Nuclear localization signalSequence analysisAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 122Ala-richAdd BLAST116
Compositional biasi383 – 439Pro-richAdd BLAST57

Sequence similaritiesi

Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAND domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri504 – 540MYND-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4333. Eukaryota.
ENOG410ZMTP. LUCA.
HOGENOMiHOG000063682.
HOVERGENiHBG051335.
InParanoidiO88450.
PhylomeDBiO88450.

Family and domain databases

Gene3Di3.10.390.10. 1 hit.
InterProiIPR000770. SAND_dom.
IPR010919. SAND_dom-like.
IPR024119. TF_DEAF-1.
IPR002893. Znf_MYND.
[Graphical view]
PANTHERiPTHR10237. PTHR10237. 1 hit.
PfamiPF01342. SAND. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
SMARTiSM00258. SAND. 1 hit.
[Graphical view]
SUPFAMiSSF63763. SSF63763. 1 hit.
PROSITEiPS50864. SAND. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88450-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSDSAAKQ LGLAEAAAVA AAAAVAAAAA AAAESEAEEP VLSRDEDSEE
60 70 80 90 100
DADSEAERET RRVTAVAVMA AESGHMDMGT EALPSPDEAA AAAAFAEVTT
110 120 130 140 150
VTVANVGSSA DNVFTTSVAN AASISGHVLS GRTALQIGDS LNTEKATLIV
160 170 180 190 200
VHTDGSIVET TGLKGPAAPL TPGPQSPPTP LAPGQEKGGT KYNWDPSVYD
210 220 230 240 250
SELPVRCRNI SGTLYKSRLG SGGRGRCIKQ GENWYSPTEF EAMAGRASSK
260 270 280 290 300
DWKRSIRYAG RPLQCLIQDG ILNPHAASCT CAACCDDMTL SGPVRLFVPY
310 320 330 340 350
KRRKKENELP TTPVKKDSPK NITLLPATAA TTFTVTPSGQ ITTSGALTFD
360 370 380 390 400
RASTVEATAV ISESPAQGDV FAGATVQEAG VQPPCRVGHP EPHYPGYQDS
410 420 430 440 450
CQIAPFPEAA LPTSHPKIVL TSLPALAVPP STPTKAVSPT VVSGLEMSEH
460 470 480 490 500
RSWLYLEEMV NSLLNTAQQL KTLFEQAKQA SSCREAAVTQ ARMQVDAERK
510 520 530 540 550
EQSCVNCGRE AMSECTGCHK VNYCSTFCQR KDWKDHQHVC GQSASVTVQA
560
DDVHVEESVI EKVAV
Length:565
Mass (Da):59,532
Last modified:November 1, 1998 - v1
Checksum:iD9C730E80A3F8492
GO
Isoform 2 (identifier: O88450-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):52,760
Checksum:iAC2A3C7EE1BFAF72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151V → D in AAC35994 (PubMed:9417089).Curated1
Sequence conflicti182A → T in AAC35994 (PubMed:9417089).Curated1
Sequence conflicti297 – 298FV → LL in AAC35994 (PubMed:9417089).Curated2
Sequence conflicti308 – 313ELPTTP → VSCPRL in AAC35994 (PubMed:9417089).Curated6
Sequence conflicti338 – 349SGQIT…GALTF → FRTDHYLWSTNL in AAC35994 (PubMed:9417089).CuratedAdd BLAST12
Sequence conflicti384 – 386PCR → LQ in AAC35994 (PubMed:9417089).Curated3
Sequence conflicti428V → A in AAC35994 (PubMed:9417089).Curated1
Sequence conflicti508G → R in AAC35994 (PubMed:9417089).Curated1
Sequence conflicti532D → V in AAC35994 (PubMed:9417089).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0059691 – 68Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055884 mRNA. Translation: AAC79679.1.
U59659 mRNA. Translation: AAC35994.1.
UniGeneiRn.11134.

Genome annotation databases

UCSCiRGD:620671. rat. [O88450-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055884 mRNA. Translation: AAC79679.1.
U59659 mRNA. Translation: AAC35994.1.
UniGeneiRn.11134.

3D structure databases

ProteinModelPortaliO88450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88450. 1 interactor.
STRINGi10116.ENSRNOP00000046656.

PTM databases

iPTMnetiO88450.
PhosphoSitePlusiO88450.

Proteomic databases

PaxDbiO88450.
PRIDEiO88450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620671. rat. [O88450-1]

Organism-specific databases

RGDi620671. Deaf1.

Phylogenomic databases

eggNOGiKOG4333. Eukaryota.
ENOG410ZMTP. LUCA.
HOGENOMiHOG000063682.
HOVERGENiHBG051335.
InParanoidiO88450.
PhylomeDBiO88450.

Miscellaneous databases

PROiO88450.

Family and domain databases

Gene3Di3.10.390.10. 1 hit.
InterProiIPR000770. SAND_dom.
IPR010919. SAND_dom-like.
IPR024119. TF_DEAF-1.
IPR002893. Znf_MYND.
[Graphical view]
PANTHERiPTHR10237. PTHR10237. 1 hit.
PfamiPF01342. SAND. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
SMARTiSM00258. SAND. 1 hit.
[Graphical view]
SUPFAMiSSF63763. SSF63763. 1 hit.
PROSITEiPS50864. SAND. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEAF1_RAT
AccessioniPrimary (citable) accession number: O88450
Secondary accession number(s): Q62998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.