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Protein

Kinesin light chain 2

Gene

Klc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.

GO - Molecular functioni

  • kinesin binding Source: BHF-UCL
  • microtubule motor activity Source: InterPro

GO - Biological processi

  • axo-dendritic transport Source: MGI
  • microtubule-based movement Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin light chain 2
Short name:
KLC 2
Gene namesi
Name:Klc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107953. Klc2.

Subcellular locationi

GO - Cellular componenti

  • ciliary rootlet Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: HGNC
  • kinesin complex Source: HGNC
  • membrane Source: MGI
  • microtubule Source: UniProtKB-KW
  • neuron projection Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Kinesin light chain 2PRO_0000215096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741PhosphoserineCombined sources
Modified residuei178 – 1781PhosphoserineCombined sources
Modified residuei443 – 4431PhosphoserineCombined sources
Modified residuei505 – 5051PhosphoserineCombined sources
Modified residuei515 – 5151PhosphoserineBy similarity
Modified residuei574 – 5741PhosphoserineBy similarity
Modified residuei575 – 5751PhosphoserineCombined sources
Modified residuei582 – 5821PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88448.
MaxQBiO88448.
PaxDbiO88448.
PeptideAtlasiO88448.
PRIDEiO88448.

PTM databases

iPTMnetiO88448.
PhosphoSiteiO88448.
SwissPalmiO88448.

Expressioni

Gene expression databases

CleanExiMM_KLC2.

Interactioni

Subunit structurei

Oligomeric complex composed of two heavy chains and two light chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk8ip3Q9ESN96EBI-301558,EBI-301496
VACWR159P686193EBI-301558,EBI-7133540From a different organism.

GO - Molecular functioni

  • kinesin binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-31518N.
IntActiO88448. 14 interactions.
MINTiMINT-136135.
STRINGi10090.ENSMUSP00000112262.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFWX-ray2.90A/B217-479[»]
ProteinModelPortaliO88448.
SMRiO88448. Positions 178-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati197 – 23034TPR 1Add
BLAST
Repeati239 – 27234TPR 2Add
BLAST
Repeati281 – 31434TPR 3Add
BLAST
Repeati323 – 35634TPR 4Add
BLAST
Repeati365 – 39834TPR 5Add
BLAST
Repeati447 – 48034TPR 6Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili78 – 14366Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the kinesin light chain family.Curated
Contains 6 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
HOVERGENiHBG006217.
InParanoidiO88448.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR002151. Kinesin_light.
IPR015792. Kinesin_light_repeat.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13374. TPR_10. 1 hit.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS01160. KINESIN_LIGHT. 2 hits.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O88448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATMVLPREE KLSQDEIVLG TKAVIQGLET LRGEHRALLA PLASHEAGEA
60 70 80 90 100
EPGSQERCLL LRRSLEAIEL GLGEAQVILA LSSHLGAVES EKQKLRAQVR
110 120 130 140 150
RLVQENQWLR EELAGTQQKL QRSEQAVAQL EEEKQHLLFM SQIRKLDEML
160 170 180 190 200
PQEEKGDVPK DSLDDLFPNE DEQSPAPSPG GGDVAAQHGG YEIPARLRTL
210 220 230 240 250
HNLVIQYASQ GRYEVAVPLC KQALEDLEKT SGHDHPDVAT MLNILALVYR
260 270 280 290 300
DQNKYKDAAH LLNDALAIRE KTLGKDHPAV AATLNNLAVL YGKRGKYKEA
310 320 330 340 350
EPLCKRALEI REKVLGKFHP DVAKQLSNLA LLCQNQGKAE EVEYYYRRAL
360 370 380 390 400
EIYATRLGPD DPNVAKTKNN LASCYLKQGK YQDAETLYKE ILTRAHEKEF
410 420 430 440 450
GSVNGENKPI WMHAEEREES KDKRRDRRPM EYGSWYKACK VDSPTVNTTL
460 470 480 490 500
RTLGALYRPE GKLEAAHTLE DCASRSRKQG LDPASQTKVV ELLKDGSGRG
510 520 530 540 550
HRRGSRDVAG PQSESDLEES GPAAEWSGDG SGSLRRSGSF GKLRDALRRS
560 570 580 590
SEMLVRKLQG GGPQEPNSRM KRASSLNFLN KSVEEPVQPG GRVFLTAAL
Length:599
Mass (Da):66,662
Last modified:November 1, 1998 - v1
Checksum:iA5E6F9081609E1CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055666 mRNA. Translation: AAC27741.1.
UniGeneiMm.271648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055666 mRNA. Translation: AAC27741.1.
UniGeneiMm.271648.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFWX-ray2.90A/B217-479[»]
ProteinModelPortaliO88448.
SMRiO88448. Positions 178-478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31518N.
IntActiO88448. 14 interactions.
MINTiMINT-136135.
STRINGi10090.ENSMUSP00000112262.

PTM databases

iPTMnetiO88448.
PhosphoSiteiO88448.
SwissPalmiO88448.

Proteomic databases

EPDiO88448.
MaxQBiO88448.
PaxDbiO88448.
PeptideAtlasiO88448.
PRIDEiO88448.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107953. Klc2.

Phylogenomic databases

eggNOGiKOG1840. Eukaryota.
COG0457. LUCA.
HOVERGENiHBG006217.
InParanoidiO88448.

Miscellaneous databases

ChiTaRSiKlc2. mouse.
PROiO88448.
SOURCEiSearch...

Gene expression databases

CleanExiMM_KLC2.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR002151. Kinesin_light.
IPR015792. Kinesin_light_repeat.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13374. TPR_10. 1 hit.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS01160. KINESIN_LIGHT. 2 hits.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins."
    Rahman A., Friedman D.S., Goldstein L.S.
    J. Biol. Chem. 273:15395-15403(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-575, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174; SER-178; SER-443; SER-505 AND SER-575, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiKLC2_MOUSE
AccessioniPrimary (citable) accession number: O88448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.